TALANTA
·
2018
·
IF 4.244
Lu Tan,Junjun Ge,Meng Jiao,Guifen Jie,Shuyan Niu
In the present work, we designed a unique enzyme-aided multiple amplification strategy for sensitive electrochemiluminescence (ECL) detection of DNA by using the amplified gold nanoparticles (GNPS)-polyamidoamine (PAMAM)-CdSe quantum dots (QDs) signal probe. Firstly, the novel GNPS-PAMAM dendrimers nanostructure with good biocompatibility and electroconductibility contains many amino groups, which can load a large number of CdSe QDs to develop amplified ECL signal probe. Then, the presence of target DNA activated the enzyme-assisted polymerization strand-displacement cycling reaction, and a large number of the hairpin template was opened. Subsequently, the opened stem further interacted with the capture hairpin (HP) DNA on the electrode, and the GNPS-PAMAM-CdSe signal probe hybridized with the exposed stem of the HP to trigger the second new polymerization reaction . Meanwhile, the first cycle was generating abundant DNA triggers which could directly open the template. As a result of the cascade amplification technique, a large number of CdSe QDs signal probe could be assembled on the electrode, generating much amplified ECL signal for sensitive detection of target DNA. Thus, this novel QDs-based amplified ECL strategy holds great promise for DNA detection and can be further exploited for sensing applications in clinical diagnostics.
研究领域:
分析化学
·
生物化学
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纳米技术
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Shuting Cheng,Huimin Liu,Hui Zhang,Guanglei Chu,Yemin Guo,Xia Sun
An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of kanamycin (KAN) was constructed by using the chemiluminescence properties of luminol-hydrogen peroxide (H 2 O 2 ) in alkaline solution. Silver nanoparticles (AgNPs) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL intensity of luminol. When the target KAN was in the presence, ECL signal decreased obviously owing to the specific interaction between KAN and aptamer. Thus, the concentration of KAN could be determined according to the intensity of ECL. To further demonstrate the effect of aptasensor, we had conducted cyclic voltammetry on an electrochemical workstation and electrogenerated chemiluminescence analyzer, respectively. Upon the optimization of assay conditions, the proposed aptasensor displayed a wide linear range from 0.5 to 100 ng mL −1 with limit of detection of 0.06 ng mL −1 . The as-proposed aptasensor was not only capable of achieving a satisfactorily low detection limit but also possessed advantages of excellent accuracy, specificity and stability. Furthermore, this constructed ECL sensing platform was successfully applied to detect KAN in real samples and it would be of excellent potential to detect other antibiotics using the proposed method.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yuanhong Xu,Tao Chen,Jing Sun,Jingquan Liu
Based on the flexible molecular engineering technique of reversible addition-fragmentation chain transfer (RAFT) polymerization, various polymers carrying positive or negative charges and different terminal groups such as pyrene or tertiary amine were synthesized for fabricating multifunctional solid-state electrochemiluminescent (ECL) sensors. Accordingly, the chemosensors immobilizing the ECL probe or co-immobilizing the ECL probe and the coreactant were realized for the quantification of small molecules (e.g., tripropylamine , tetracycline), and an aptasensor was developed for the specific and sensitive lysozyme assay (limit of detection: 0.1 ng/mL). All of the sensors were realized via a simple design exploiting the π−π stacking and electrostatic interactions . It was confirmed that the proposed strategy is simple but universal for the fabrication of versatile ECL sensors that showed simplicity, cost-effectiveness, high sensitivity, long-term stability, and excellent reproducibility.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
聚合物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yan Xu,Zhenbo Wang,Caifeng Ding,Xiliang Luo
It is a great challenge to detect the target selectively and accurately in complex biological samples. A ratiometric signal amplification strategy based on antifouling peptide is proposed to detect thrombin (TB) in this work. The electrode is modified with conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and [email protected] Then the bi-functional peptide anchors to the modified electrode and captures the low toxic electrochemiluminescence (ECL) probe. The bi-functional peptide includes a new antifouling sequence (DRDRDRDR) and a TB recognizable sequence (SGRPVLG), while the former can greatly reduce the non-specific adsorption in complex samples and the latter can be cleaved by TB. The new self-enhanced luminescence probe labeled as PAMAM-QDs is composed of low toxic and water-soluble CuInZnS/ZnS quantum dots (CIZS/ZnS QDs) and polyamidoamine (PAMAM) via amide bonds. Concentration of TB can be obtained via the ratio of the signals of ECL QDs /ECL [email protected] accurately and sensitively. The ECL biosensor with the detection line 1.82 fM can work from 10 fM to 1 nM. Thanks to the antifouling property of the designed peptide and the amplification of the new ECL probe, the biosensor can be used for quantifying TB in practical complex samples with high selectivity and sensitivity.
研究领域:
分析化学
·
生物传感器
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纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zirui Wang,Jilong Zheng,Min Li,Shujia Wang,Zhida Gao,Yan-Yan Song,Chenxi Zhao
Rapid and accurate detection of trace microbial pathogens is essential for human health, environmental monitoring, and food safety. Here, we report the development of a solid-state electrochemiluminescence (ECL) bacterial sensor based on a β-glycosidase (β-Gal)-triggered coreactant generation strategy. Leveraging a three-dimensional TiO2 nanotube array integrated with peroxidase-like MIL-53(Fe), glucose oxidase-like Au nanoparticles, and luminol, this sensor efficiently generates coreactants on-site, enabling sensitive glucose detection. As glucose is the primary product of β-Gal-catalyzed lactose hydrolysis, its concentration directly correlates with β-Gal enzymatic activity, thus allowing for the quantification of β-Gal via ECL signal intensity. Using enterohemorrhagic Escherichia coli (EHEC) as a model of food-borne pathogens, the sensor demonstrates rapid detection (30 min) with an ultralow LOD (0.8 CFU mL–1), representing the most sensitive label-free bacterial ECL sensor reported to date. Furthermore, the ECL electrode was applied for the detection of EHEC on raw salmon slices, underscoring its potential for food safety monitoring.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
微生物学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Ruoqi Wang,Qiao Zhang,Lei Yu,Yue Sun,Yunwei Gao,Dazhong Shen
Pre-oxidation is a simple and effective strategy to enhance the electrochemiluminescence (ECL) of Au nanoclusters. But the pre-oxidation ECL enhancement mechanism is still not clear. Herein, an in-situ formation immobilized coreactant promoters mechanism was proposed and verified by using Eu-doping methionine-stabilized Au nanoclusters (Met-AuEuNCs) as the model, with the ECL intensity 3.72-fold of that of Met-AuNCs. The ECL of Met-AuEuNCs was further enhanced 24.3-fold via pre-oxidation in 100 mM triethanolamine (TEOA) at the potential step of 1.0 V for 60 s, obtained a high ECL quantum yield of 143 %. The pre-oxidized Met-AuEuNCs reserved 20.1-fold ECL enhancement ratio after storing for 12 h and generated intense self-ECL in the absence of coreactant. Immobilized coreactant and coreactant promoters were formed in situ during pre-oxidation stage and enhanced the ECL of Au nanoclusters. A low potential step (e.g., 0 V, 5 s) was useful to activate the immobilized coreactant promoters for higher ECL enhancement ratio. The pre-oxidation enhancement strategy is valid only in the presence of anodic coreactants and Au nanoclusters. The pre-oxidation mechanism was applied in molecularly imprinted polymer (MIP) based ECL sensor, using neuron specific enolase (NSE) as the model analyte, with the detection limit of 6.3 fg mL −1 . The re-binding of the MIP film to NSE blocked the accesses between Met-AuEuNCs and TEOA in the pre-oxidation, activation and ECL measurement, decreased the ECL of Met-AuEuNCs. The pre-oxidized MIP-ECL sensor offered higher sensitivity than that in normal MIP-ECL mode without pre-oxidation and activation treatments.
研究领域:
分析化学
·
纳米技术
·
电化学
·
生物传感器
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Meng Li,Hongmei Yang,Chao Ma,Yan Zhang,Shenguang Ge,Jinghua Yu,Mei Yan
In this paper, a novel and sensitive signal-off electrochemiluminescence (ECL) aptasensor was proposed for the detection of adenosine triphosphate (ATP) on a nanoporous gold (NPG) modified glassy carbon electrode. The NPG was easily synthesized by a selective dissolution of silver from silver/gold alloy in nitric acid . The ECL aptasensor consisted of two main elements: an ECL substrate, made by modifying the amino group functionalized Ru(bpy) 3 2+ -doped silica (Ru-silica), and an ECL intensity switch, which contains two probes, split from one aptamer. The first probe (ssDNA1) was functionalized with a thiol group at one end and attached to Ru-silica through the linking agent of N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. The other one (ssDNA2) is ferrocene-labeled probe, which was functionalized with ferrocene tag at the amino group functionalized end. We demonstrated that the ssDNA1/ATP/ssDNA2 sandwich composites layer could be formed in the presence of ATP, and lead to a quenching of Ru(bpy) 3 2+ . However, only a slight decrease could be observed in the absence of ATP. Under optimal conditions, the proposed biosensor provided a wide linear response range over 0.1 pmol L −1 –10.0 nmol L −1 with a detection limit of 0.03 pmol L −1 .
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Qingqing Zhang,Dandan Hu,Tianyu Zhan,Yufang Hu,Zhiyong Guo,Sui Wang
A novel Medusa electrochemiluminescence (ECL) immunosensor for quantitative detection of histone acetyltransferase (HAT p300) is designed and built well based on magnetic gold nanoparticles (Au-Fe 3 O 4 NPs), DNA nanonets and terminal deoxynucleotidyl transferase (TdT)-triggered renascent DNAs containing abundant A and T (named as AT-dsDNAs). Firstly, Au-Fe 3 O 4 NPs and substrate peptides are coated with the bare electrode surface one after the other. As the acetylation reaction happens, DNA nanonets, which are synthesized via Y-shape DNA (as building block) and acetyl antibody (Ab Ac )-grafted dsDNA (as crosslinker), are attached on the electrode surface. Next, AT-dsDNAs are formed under NaCl condition by TdT catalysis, in where deoxyadenosine triphosphate (dATP) and deoxythymidine triphosphate (dTTP) (half and half) can be added ceaselessly to countless 3′−OH of DNA nanonets. Abundant Ru(phen) 3 2+ molecules, which can be integrated well into high-level DNA nanomaterials above, serve as ECL luminophore in our biosensing. Only being HAT p300, an outstanding ECL response occurs favorably, and a detection limit of 0.0029 nM is acquired with a linear response in the range of 0.006–60 nM. The Medusa immunosensor with improved sensitivity presents a promising and possible widely use for the HATs-relevant clinical study and drug development.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物传感器
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xinxia Wang,Wei Yuan,Zhilan Sun,Fang Liu,Daoying Wang
Pseudomonas aeruginosa ( P. aeruginosa ) is an opportunistic infection-causing pathogen that threatens human health. Accordingly, a rapid and ultrasensitive analytical method is required urgently. Herein, an ultrasensitive multicolor electrochromic sensing platform was established on the basis of a closed bipolar electrode (BPE). Prussian blue (PB), the blue spot that can be easily electrodeposited, was selected as an electrochromic indicator at the closed BPE cathode. Integrating with the anodic emitter, Ru(bpy) 3 2+ , which emitted optical red light, visualized multicolor electrochromism was achieved on closed BPE. Particularly, physical separation between the positive and negative poles of the closed BPE greatly prevented mutual interference between Ru(bpy) 3 2+ and PB. Consequently, the sensitivity and accuracy of the proposed biosensor considerably improved. Notably, owing to magnetic-separation technology, the closed BPE surface required no modification. Without any complex pretreatment, the entire experiment time could be greatly shortened because the [email protected] completely captured P. aeruginosa in food matrix within only 20 min. By comparing the visual electrochromic colors, ultrasensitive screening of P. aeruginosa was accomplished within 1–10 8 CFU mL −1 . Combining the merits of closed BPE, electrochemiluminescence (ECL), and electrochromic, this strategy provided an accurate and intrinsic way for visual detection of P. aeruginosa , as well as great potential in measuring other pathogens.
研究领域:
生物技术
·
分析化学
·
微生物学
·
传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xiaolin Yang,Yuxi Wei,Zimei Wang,Junxia Wang,Honglan Qi,Qiang Gao,Chengxiao Zhang
This work reports a highly efficient electrogenerated chemiluminescence (ECL) quenching on lipid-coated multifunctional magnetic nanoparticles (MMNP) for the determination of proteases incorporating membrane-confined quenching with a specific cleavage reaction for the first time. A new ruthenium complex [Ru(bpy)2(ddcbpy)](PF6)2 (bpy = 2,2′-bipyridine, ddcbpy = 4,4′-didodecyl-carbonyl-2,2′-bipyridine with two hydrophobic long alkyl chains) was synthesized as a signal probe, while [cholesterol-(CH2)6-HSSKLQK(peptide)-ferrocene (quencher)] was designed as a specific peptide-quencher probe. The MMNP were prepared by inserting both the signal probe and the peptide-quencher probe into the cholesterol–phospholipid-coated Fe3O4 magnetic nanoparticles (Fe3O4 NP, ∼200 nm). When prostate specific antigen (PSA) taken as a model analyte was introduced into the suspension of MMNP, PSA cleaved the amide bond of SK in cholesterol-(CH2)6-HSSKLQK-Fc, and then the cleaved peptide-motif-Fc-quencher was deviated from the MMNP, resulting in the increase in the ECL intensity. It was found that the ECL quenching constant of [Ru(bpy)2(ddcbpy)]2+ on MMNP (KSV, NP/lipECL =2.68 × 107 M–1) is 137-folds higher than that on the lipid-coated electrode (KSV, lipECL=1.95 × 105 M–1) and 391-folds higher than that in the solution (KSV, aqECL =6.86 × 104 M–1). The ECL emission of Ru(bpy)32+ derivative-attached Fe3O4 NP was observed at ∼1.2 V, involving the tunnel-electron transfer pathway (TPA+ Ru(bpy)33+ = Ru(bpy)32+*). Based on the highly efficient ECL quenching of the ruthenium complex by ferrocene on the MMNP, a new ECL method was developed for PSA with a linear range from 0.01 to 1.0 ng/mL and a limit of detection of 3.0 pg/mL. This work demonstrates that the approach of ECL quenching by ferrocene on lipid-coated Fe3O4 NP is promising and could be easily extended to determine other proteases.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Shuting Cheng,Rui Xu,Fengzhen Yang,Jingcheng Huang,Xia Sun,Xue Huang,He Li,Falan Li,Yemin Guo,Mohammad Hasanzadeh,Yelong Zhu
A novel sandwich electrochemiluminescence (ECL) aptasensor was developed for highly sensitive detection of kanamycin using luminol-functionalized aptamer as a signal probe. The aptasensor used polyethyleneimine (PAMAM), molybdenum disulfide, and multi-walled carbon nanotubes as the substrate, which provided enough binding sites for aptamer1 (the aptamer which modified NH 2 ) coupling. We found that kanamycin could be detected using the aptamer1 containing the same base sequence as aptamer2 (the aptamer which modified SH) on the electrode self-assembly. In addition, PAMAM nanocomposites can be used to effectively improve the ECL intensity by loading a high volume of luminol molecules and silver nanoparticles. In the presence of kanamycin, the sandwiched aptasensor was formed between aptamer1 and the probe of aptamer2 connecting silver nanoparticles, luminol, and PAMAM, resulting in a proportional increase of ECL intensity. Since the significantly enhanced loading of luminol by PAMAM accelerated the electron transfer, the sensitive aptasensor exhibited a wide linear range of detection from 1 × 10 -3 to 1 × 10 3 ng/mL and a low detection limit of 0.21 pg/mL (S/N) for kanamycin. The fabricated aptasensor was successfully applied in quantitative analysis of kanamycin in milk samples.
研究领域:
分析化学
·
电化学
·
生物传感器
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2019
·Vol. 23
·期 8
·2579-2588
·
IF 2.531
Du Fangkai,Zhang Hui,Tan Xuecai,Ai Chenhao,Li Mengru,Yan Jun,Liu Min,Wu Yeyu,Feng Defen,Liu Shaogang,Han Heyou
A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO 2 ) nanocomposite labeling as an enhancer. Ru(bpy) 3 2+ -Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO 2 , coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO 2 nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO 2 on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）