TALANTA
·
2019
·
IF 4.916
Wei He,Xiangying Sun,Bin Liu,Jiangshan Shen
In this paper, deoxyribonucleic acid (DNA) with different lengths were used to control the distance between carbon dots (CDs) and silver nanoparticles (AgNPs) in self-assembled multilayers (SAMs). Surface-enhanced fluorescence and fluorescence resonance energy transfer (SEF-FRET) could be achieved based on changing DNA strands. The fluorescence intensity of CDs SAMs with 6-base DNA strands could be enhanced up to ca. 5.6 times by AgNPs. As-fabricated CDs SAMs with excellent luminescent properties, superior stability have been employed for the development of a label-free fluorescence sensing platform for DNA detection. Since DNA would hybridize with the complemented one which was attached on the surface of SAMs, resulting in a close distance between CDs and AgNPs, FRET could thus occur between AgNPs and CDs, resulting in quenching the fluorescence of CDs SAMs. This sensitive sensing platform could show excellent analytical performance for detecting DNA with a linear response ranging from 93.07 pM to 5.433 nM and a detection limit of 16.36 pM, which could be further employed to probe human blood samples. This could prove a promising method for the detection of DNA.
研究领域:
生物化学
·
纳米技术
·
分子生物学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Cheng Xia,Cen Yao,Xu Guanhong,Wei Fangdi,Shi Menglan,Xu Xiaoman,Sohail Muhammad,Hu Qin
The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via π-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.
Schematic of a novel fluorometric ATP assay based on the fluorescence resonance energy transfer (FRET) between aptamer modified carbon dots (CD-aptamer) and graphene oxide (GO). CD-aptamer was used as the energy donor and molecular recognition probe, and GO acted as energy acceptor. This assay exhibits high sensitivity and selectivity with a detection limit as low as 80 pM.
研究领域:
分析化学
·
生物传感器
·
纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2013
·
IF 2.144
Lijuan Feng,Cuiyan Tong,Yao He,Bingxin Liu,Chunyu Wang,Jie Sha,Changli Lü
A new fluorescent sensor based on single water-soluble β-cyclodextrin (β-CD) molecule as the support carrier of donor and acceptor complex was designed, and used for TNT detection by fluorescence resonance energy transfer (FRET). In this sensing platform, per-6-amino-β-CD (per-6-NH 2 -β-CD) was used as the detection vehicle. The probe dye of fluorescein 5(6)-isothiocyanate (FITC) was covalently linked onto the per-6-NH 2 -β-CD rim with the grafting molar ratio of 1:1, the residual amino groups of per-6-NH 2 -β-CD can adsorb TNT molecules by forming Meisenheimer complex (TNT–amine complex) with TNT. The absorbtion spectrum of this complex has a spectral overlapping with the emission of FITC in aqueous solution, so it can strongly suppress the fluorescence emission of the adjacent FITC through FRET on β-CD vehicle. This FRET-based fluorescent sensing technique provides a facile, ultrasensitive and selective detection method for TNT molecule. The observed linear fluorescence intensity change could allow the quantitative detection TNT with the detection limit of 20 nM in water.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Nuo Duan,Kexin Ren,Mingqian Song,Xinyue Zhang,Zhouping Wang,Fei Jia,Shijia Wu
The presence of biogenic amines (BAs) in food is not only important from a toxicological view, but can also be used as an indicator of food spoilage. However, the design of biosensors for the detection of BAs is very limited. Hence, in this work, a dual-donor fluorescence resonance energy transfer (FRET) aptasensor was established for simultaneous determination of histamine (HIS) and tyramine (TYR) using dual-color quantum dots (QDs) as energy donors and two-dimensional molybdenum sulfide nanosheets (MoS 2 NSs) as energy acceptor. Specific aptamer for HIS (Apt 1) and TYR (Apt 2) were labeled with two fluorescence emission QDs (QD 530 and QD 625 ), respectively. On the basis of the excellent adsorption ability of MoS 2 NSs toward aptamer, the fluorescence quenching can be observed between MoS 2 NSs and QD 530 -Apt1 and QD 625 -Apt2. Upon the addition of HIS and TYR, the aptamer conformation switches induced by the target binding mitigate the interaction force of MoS 2 NSs and QD 530 -Apt1 and QD 625 -Apt2, resulting in the fluorescence recovery. The method displays good sensitivity with limit of detection (LOD) of 0.1 ng/mL for HIS and 0.25 ng/mL for TYR, respectively. It can also be applied to the HIS and TYR detection in spiked fish samples with recovery of 95.2%∼106.6%, demonstrating its prospect in the sensitive detection of BAs in animal-derived foodstuff.
研究领域:
食品科学
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分析化学
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纳米技术
·
生物技术
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DOI
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 35
·6752-6757
·
IF 7.571
Chengyi Hong,Jingjing Wang,Yuying Wang,Zhiyong Huang,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan
β-Lactoglobulin (β-Lg), a food allergen, can easily cause allergic reactions in infants and young children. Therefore, it is necessary to develop a rapid, sensitive, and selective detection method to protect individuals prone to allergies. In this paper, a fluorescence assay based on WS2 nanosheets and a fluorescent dye (FAM)-labeled β-Lg aptamer was designed to detect β-Lg rapidly with high sensitivity. In the sensing platform, the β-Lg aptamer is adsorbed on the WS2 nanosheet surface by van der Waals forces, which trigger the phenomenon of fluorescence resonance energy transfer (FRET) and suppress the fluorescence signal in the system. When β-Lg is present, the conformation of the aptamer specifically bound to β-Lg changes. Therefore, the aptamer is separated from the WS2 nanosheet surface, and the fluorescence signal is recovered. This method combines the high quenching efficiency of WS2 nanosheets and good specificity of the β-Lg aptamer. The detection range of this method for β-Lg is 0.1–100 μg mL−1. The detection limit is 20.4 ng mL−1. This method exhibits high sensitivity, selectivity and good reproducibility, and it can be used for β-Lg detection in actual samples.
研究领域:
食品科学
·
分析化学
·
纳米技术
·
生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Yanfei He,Dongdong Zhang,Qiong Wu,Gengan Du,Ruoting Liu,Xianqing Zhou,Yurong Zhang
Aflatoxin B1 (AFB1) has strong carcinogenicity, mutagenicity, and teratogenicity even at low concentrations, presenting a major risk to food safety and human health, hence, it is crucial to develop a sensitive detection technique for AFB1. Consequently, cadmium telluride (CdTe) quantum dots conjugated with AFB1 aptamers serve as fluorescent signal probes, whereas Fe 3 O 4 @UiO-66-NH 2 nanocomplexes are employed as magnetic carriers and fluorescence quenchers. Fe 3 O 4 @UiO-66-NH 2 reduces background signal interference, thereby enhancing detection sensitivity and Förster Resonance Energy Transfer (FRET) efficiency. We have developed a highly sensitive and selective magnetron fluorescence aptasensor based on FRET for the specific detection of AFB1. Under the ideal experimental conditions, the linear range of AFB1 is 0.001–200 ng/mL, with a linear equation of y = 0.16 x +0.65 (R 2 = 0.996), and the detection limit is 0.012 ng/mL. The aptasensor demonstrated superior selectivity, reproducibility, and stability for AFB1, the recoveries for real samples of corn and wheat ranged from 95 % to 103 %, demonstrating the feasibility and practicality of the sensor. Furthermore, the accuracy of the aptasensor was verified by comparison with the results of high performance liquid chromatography, indicating that the aptasensor holds significant potential for practical detection applications.
研究领域:
食品安全
·
分析化学
·
生物传感
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DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-7
·
IF 3.125
Wang Shan,Liu Ruiqing,Li Chenchen
In the presence of Hg 2+ , a fluorescence resonance energy transfer (FRET) system was constructed between CdSe quantum dots (QDs) (donor) and g-C 3 N 4 (receptors). Nanocomposites of g-C 3 N 4 supported by CdSe QDs (CdSe QDs/g-C 3 N 4 nanosheets) were fabricated through an electrostatic interaction route in an aqueous solution. The nanocomposites were characterized by X-ray photoelectron spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Results showed that the g-C 3 N 4 nanosheets were decorated randomly by CdSe QDs, with average diameter of approximately 7 nm. The feasibility of the FRET system as a sensor was demonstrated by Hg (II) detection in water. At pH 7, a linear relationship was observed between the fluorescence intensity and the concentration of Hg (II) (0–32 nmol/L), with a detection limit of 5.3 nmol/L. The new detection method was proven to be sensitive for detecting Hg 2+ in water solutions. Moreover, the method showed high selectivity for Hg 2+ over several metal ions, including Na + , Mg 2+ , Ca 2+ , Pb 2+ , Cr 3+ , Cd 2+ , Zn 2+ , and Cu 2+ . The CdSe QDs/g-C 3 N 4 nanosheet conjugate exhibited desirable long-term stability and reversibility as a novel FRET sensor. The novel FRET-based fluorescence detection provided an attractive assay platform for quantifying Hg 2+ in complex water solutions.
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 37
·11483-11492
·
IF 6.641
Yongxin Fu,Hui Jin,Xiangning Bu,Rijun Gui
In this work we prepared novel magnetic and fluorescent dual-functional nanohybrids that consisted of Fe3O4 nanoparticles (NPs) as the substrate, blue-emitting carbon dots (B-CDs) combined on the surface of Fe3O4 NPs, and phycoerythrin (PE) imprinted silica grown on the surface of an Fe3O4 NP/B-CD complex. Under optimal conditions, Fe3O4/B-CD/PE-imprinted SiO2 nanohybrids (MIP-based nanohybrids) were prepared and their fluorescence (FL) properties were researched. The addition of PE induced an FL decrease of B-CDs, together with an FL increase of PE. The changes were due to FRET from B-CDs to PE, because there is a large overlap between the UV-vis absorption spectrum of PE and the FL emission spectrum of B-CDs. Meanwhile, the PE-induced inner filter effect of B-CDs also led to FL quenching of B-CDs. PE was captured into specific cavity sites of PE-imprinted SiO2 shell layers on MIP-based nanohybrids to generate MIP-PE nanohybrids. Therefore, PE contacted B-CDs or was spatially close to B-CDs. There is a well-plotted linear relationship between FL peak intensity ratios (IPE/IB-CDs and I605/I450) and PE concentrations in the range of 5–200 ng mL−1, with a low limit of detection of 1.5 ng mL−1. This developed sensor showed selective and sensitive responses towards PE, over other interferents. In real samples, this sensor had excellent capacity for PE determination and superior detection performances.
研究领域:
分析化学
·
生物传感
·
纳米技术
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Hongliang Tan,Xiayi Wu,Yuhao Weng,Yajie Lu,Zhen-Zhong Huang
Metal–organic framework (MOF) has been extensively explored in a number of fields due to its diverse properties. In this work, we demonstrated the potential of MOF in the establishment of a self-assembled fluorescence resonance energy transfer (FRET) system for developing ratiometric fluorescent nanoprobe. For this purpose, zeolitic imidazolate framework-8 (ZIF-8) was selected as a MOF model to entrap carbon dot (CD) and curcumin (CCM) during its self-assembly, which produces CD/CCM@ZIF-8. Benefiting from the confinement effect of ZIF-8, the loaded CD and CCM can be brought in close proximity for energy transfer to occur. Under optimal conditions, a high FRET efficiency of 68.7% can be obtained. Importantly, compared with traditional FRET systems, the fabrication process of CD/CCM@ZIF-8 is much more simple and straightforward, which does not involve the elaborate design and complicated synthesis of molecular linkers. However, in the presence of hypochlorous acid (HClO), the FRET process from CD to CCM will be disrupted, rendering CD/CCM@ZIF-8 to display a ratiometric response to HClO. This finding led to a method for ratiometric fluorescent detection of HClO with a detection limit of 67 nM and excellent selectivity over other reactive oxygen species. We believe that this study can give a new insight into the rational design and application of FRET-based nanoprobes.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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DOI
高影响因子
Acta Pharmaceutica Sinica B
·
2018
·
IF 6.014
Xia Lv,Shuguang Zhang,Huipeng Ma,Peipei Dong,Xiaodong Ma,Ming Xu,Yan Tian,Zeyao Tang,Jinyong Peng,Haibo Chen,Jianbin Zhang
Microemulsions are promising drug delivery systems for the oral administration of poorly water-soluble drugs. However, the evolution of microemulsions in the gastrointestinal tract is still poorly characterized, especially the structural change of microemulsions under the effect of lipase and mucus. To better understand the fate of microemulsions in the gastrointestinal tract, we applied small-angle X-ray scattering (SAXS) and fluorescence resonance energy transfer (FRET) to monitor the structural change of microemulsions under the effect of lipolysis and mucus. First, the effect of lipolysis on microemulsions was studied by SAXS, which found the generation of liquid crystalline phases. Meanwhile, FRET spectra indicated micelles with smaller particle sizes were generated during lipolysis, which could be affected by CaCl 2 , bile salts and lecithin. Then, the effect of mucus on the structural change of lipolysed microemulsions was studied. The results of SAXS and FRET indicated that the liquid crystalline phases disappeared, and more micelles were generated. In summary, we studied the structural change of microemulsions in simulated gastrointestinal conditions by SAXS and FRET, and successfully monitored the appearance and disappearance of the liquid crystalline phases and micelles.
研究领域:
药剂学
·
生物物理学
·
药物递送系统
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DOI
PubMed
高影响因子
Analytica
·
2022
·Vol. 3
·期 4
·385-393
·
IF 0
Zhenlong Han,Pengfei Du,Ronghui Wen,Baoshan Chen,Xipu He
A fluorescence off-on method for the detection of Sorghum mosaic virus (SrMV) based on fluorescence resonance energy transfer (FRET) was developed in this study. The biosensor was realized by the adsorption of SrMV coat protein (CP) on gold nanoparticle (AuNP) and conjugation of anti-SrMV antibody (Ab) on cadmium telluride quantum dot (CdTe QD). The optimum quenching efficiency was about 50% obtained at a CdTe QD-Ab/AuNP-CP ratio of 1:7. Moreover, the feasibility of the developed biosensor was verified by the detection of purified CP and the limit of detection was estimated as 0.02 μg/mL. This strategy was also successfully applied to monitor SrMV CP in plant sap with a recovery rate between 97.7 and 107.4%. The developed biosensor is a simple, rapid, and efficient technique which does not need excessive washing and separation steps.Keywords:Sorghum mosaic virus;quantum dot;AuNP;FRET;immune complex
研究领域:
分析化学
·
植物病理学
·
生物传感器技术
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DOI
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zhongfei Shang,Meimei Wang,Jiguang Li,Zhenzhen Cai,Luyan Yang,Xincun Dou,Baiyi Zu
The design and development of ultra-accurate probe is of great significance, but there is still a big challenge for chemical sensing in complex practical detection scenes. Here, a unique optical nanoprobe with high sensitivity and anti-interference capability toward perchlorate is designed by exactly matching the FRET energy of the functionalized ligand with the green emission of the UCNPs. Benefiting from the design of specific recognition sites and the intrinsic superiority of the FRET mechanism, the nanoprobe demonstrates an ultralow detection limit of 1.6 nM, rapid response within 4 s, and excellent anti-interference ability even in the presence of 22 potential interferents. The reliable detection performance in complicated real cases is further verified in recognizing trace perchlorate from interferents with various fluorescent emissions and colors existing simultaneously. The proposed design strategy of nanoprobe is expected to open up a brand new methodology for ultra-accurate sensing.
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高影响因子
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