International Journal of Nanomedicine
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2025
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IF 6.5
Yong Lian,Ning Ma,Qianying Cheng,Mingquan Luo,Zhen Xu,Fei He,Xiaomei Zhou,Ying Zhang,Dejun Jin,Yidan Kong,Yong Wang,Na Wei
Purpose Study aims to optimize the synthesis conditions for silver nanoparticle composites [ALP(D)-AgNPs] using a rapid and environmentally friendly method and investigate the antioxidant, antibacterial, and anticancer activities of the fabricated composite.Methods The polysaccharide component ALP-D was extracted and purified from the fruits of Amomum longiligulare and subsequently used for further experiments. The structure of ALP-D was characterized by FT-IR, monosaccharide composition and molecular weight.The optimal conditions for the green synthesis of silver nanoparticles using ALP-D were determined through single-factor experiments. The synthesized silver nanoparticles were characterized by UV-Vis spectroscopy, FT-IR, DLS, HRTEM and XRD. Finally, the in vitro antioxidant activity, antibacterial activity and anticancer activity of the nano-silver composite were evaluated.Results Single-factor experiments identified the optimal synthesis conditions for ALP(D)-AgNPs as a reaction time of 180 min, a temperature of 100 °C, and a 10:1 volume ratio of silver nitrate to ALP-D. The free radical scavenging activity of ALP(D)-AgNPs against DPPH and ABTS was significantly enhanced compared with that of ALP-D. The minimum inhibitory concentration (MIC) values of ALP(D)-AgNPs against E coli and B subtilis were 31.25 μg/mL, while the MIC value against S aureus was 62.5 μg/mL. The minimum bactericidal concentration (MBC) values of ALP(D)-AgNPs were 125 μg/mL for E coli, B subtilis, and S aureus. The IC50 values of ALP(D)-AgNPs on the MDA-MB-231, HepG2, Caco-2, and C6 cancer cell lines were 14.72 ± 0.23, 8.19 ± 0.65, 22.73 ± 3.01, and 15.77 ± 2.91 μg/mL, respectively.Conclusion In summary, we have identified a novel material for the green synthesis of silver nanoparticles. The results show the ALP(D)-AgNPs synthesized using the new material ALP-D exhibit excellent stability and dispersibility. Furthermore, the biological activity reveals that ALP(D)-AgNPs possess notable antioxidant, antibacterial, and cancer-suppressing activities.
研究领域:
材料科学
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生物化学
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药理学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Tongxin Zhou,Huili Wang,Qian Han,Zhaoping Song,Dehai Yu,Guodong Li,Wenxia Liu,Cuihua Dong,Shaohua Ge,Xiao Chen
Sodium alginate (SA) is widely used in the food, biomedical, and chemical industries due to its biocompatibility, biodegradability, and excellent film-forming properties. This article introduces a simple method for preparing uniform alginate-based packaging materials with exceptional properties for fruit preservation. The alginate was uniformly crosslinked by gradually releasing calcium ions triggered by the sustained hydrolysis of gluconolactone (GDL). A cinnamaldehyde (CA) emulsion, stabilized by xanthan without the use of traditional surfactants, was tightly incorporated into the alginate film to enhance its antimicrobial, antioxidant, and UV shielding properties. The alginate-based film effectively blocked ultraviolet rays in the range of 400–200 nm, while allowing for a visible light transmittance of up to 70 %. Additionally, it showed an increased water contact angle and decreased water vapor permeability. The alginate-based film was also employed in the preparation of coated paper through the commonly used coating process in the papermaking industry. The alginate-based material displayed excellent antioxidant properties and antimicrobial activity against Escherichia coli , Staphylococcus aureus and Botrytis cinerea , successfully extending the shelf life of strawberries to 7 days at room temperature. This low-cost and facile method has the potential to drive advancements in the food and biomedical fields by tightly incorporating active oil onto a wide range of biomacromolecule substrates.
研究领域:
食品科学
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材料科学
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生物技术
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包装工程
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DOI
PubMed
高影响因子
Foods
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2024
·Vol. 13
·期 5
·793
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IF 4.7
Mingming Zhao,Zhiheng You,Huayun Chen,Xiao Wang,Yibin Ying,Yixian Wang
Artificial scent screening systems, inspired by the mammalian olfactory system, hold promise for fruit ripeness detection, but their commercialization is limited by low sensitivity or pattern recognition inaccuracy. This study presents a portable fruit ripeness prediction system based on colorimetric sensing combinatorics and deep convolutional neural networks (DCNN) to accurately identify fruit ripeness. Using the gas chromatography–mass spectrometry (GC-MS) method, the study discerned the distinctive gases emitted by mango, peach, and banana across various ripening stages. The colorimetric sensing combinatorics utilized 25 dyes sensitive to fruit volatile gases, generating a distinct scent fingerprint through cross-reactivity to diverse concentrations and varieties of gases. The unique scent fingerprints can be identified using DCNN. After capturing colorimetric sensor image data, the densely connected convolutional network (DenseNet) was employed, achieving an impressive accuracy rate of 97.39% on the validation set and 82.20% on the test set in assessing fruit ripeness. This fruit ripeness prediction system, coupled with a DCNN, successfully addresses the issues of complex pattern recognition and low identification accuracy. Overall, this innovative tool exhibits high accuracy, non-destructiveness, practical applicability, convenience, and low cost, making it worth considering and developing for fruit ripeness detection.
研究领域:
食品科学
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人工智能
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植物生理学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Mingming Zhao,Huizi Lu,Zhiheng You,Huayun Chen,Xiao Wang,Yaqing Zhang,Yixian Wang
Developing a colorimetry-based artificial scent screening system (i.e., an olfactory visual sensing system) with high sensitivity and accurate pattern recognition for detecting fruit ripeness remains challenging. In this work, we construct a flexible dye/CelluMOFs-based sensor array with improved sensitivity for on-site detection of characteristic gases of fruits and integrate a densely connected convolutional network (DenseNet) into the sensor array, enabling it to recognize unique scent fingerprints and categorize the ripeness of fruits. In the system, CelluMOFs are synthesized through in situ growth of γ-cyclodextrin metal–organic frameworks (γ-CD-MOFs) on flexible fiber filter paper to fabricate a uniform, flexible and porous dye/CelluMOFs sensitive membrane. Compared to the pristine filter paper, the CelluMOFs exhibit increased porosity with a 62 times higher specific surface area and a 3-fold increase in dye loading capacity after 12 h of adsorption. The prepared dye/CelluMOFs sensing film shows outstanding mechanical and detection stability with negligible deviation after 100 cycles of rubbing. The colorimetric visualization arrays with multiple colorimetric dye/CelluMOFs chips, enable the sensitive recognition and detection of nine kinds of characteristic fruit odors and achieve a high response at 8–1500 ppm of trans-2-hexenal, showcasing remarkably low gas detection thresholds. On the basis of the ppm-level limit of detection with high sensitivity, the fabricated colorimetric sensor arrays are typically used for in situ assessment of fruit ripeness by integrating DenseNet. This approach achieves a satisfactory classification accuracy of 99.09% on the validation set, enabling high-precision prediction of fruit ripeness levels.
研究领域:
食品科学
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材料科学
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人工智能
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Ping Shao,Haoya Zhang,Ben Niu,Ligang Jiang
It was previously found that R-(+)-Limonene emulsion could be stabilized by Ulva fasciata polysaccharide . In the present study, emulsions of R-(+)-Limonene were developed for coating of strawberries to improve the shelf life and microbiological safety. Edible coatings with at least 0.15% w/w of R-(+)-Limonene improved the microbial stability of the strawberries, resulted effective in the decontamination of external pathogens such as Escherichia coli , Bacillus subtilis and Staphylococcus aureus . Changes in weight loss, total soluble solid (TSS), pH, total phenolic, ascorbic acid , textural properties and surface color of strawberries during storage were evaluated. R-(+)-Limonene emulsions were effective for decreasing water loss of all the fruit samples tested. The decrease in TSS values was higher in the control strawberries. Initial decreases followed by increases of pH during storage of strawberries. The amount of total phenols detected in control fruit sharply increased during the first 6 days then remained steady, whereas the total soluble phenols content of treated strawberries gradually increased during storage. Strawberries coated with emulsion had higher L* and a* values as compared to control fruits. The R-(+)-Limonene coatings have demonstrated the potential to inhibit foodborne pathogen contamination of strawberries, and prolong their shelf life.
研究领域:
食品科学
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植物化学
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微生物学
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DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
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2023
·Vol. 32
·期 14
·2093-2104
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IF 2.9
Sun Yuhao,Hu Yaru,Hu Danqi,Xiao Zhuqian,Wang Hongpeng,Huang Jun,Mao Jianwei
Three different Akebia trifoliata fruit juices were prepared, and their effects on modulation of human fecal microbiota were elucidated through an anaerobic fermentation in vitro. Results indicated that the introduction of inoculatedly-fermented Akebia trifoliata fruit juice promoted short-chain fatty acids productivity. Fecal microbiota analysis demonstrated up-regulations for abundances of Limosilactobacillus , Megamonas , Bifidobacterium , and Escherichia_Shigella , and down-regulations for numbers of Bacteroides , Prevotella_9 , Parasutterella , and Sutterella . Correlation analysis confirmed relationships among sample components, short-chain fatty acids productivity, and microbial abundances, suggesting that sugars and organic acids stimulated growth of Actinobacteriota and suppressed proliferation of Proteobacteria , thus uncovering the underlying mechanism for the better ability of inoculatedly-fermented Akebia trifoliata fruit juice to regulate microbiota structure. Besides, clusters of orthologous groups of proteins analysis indicated that metabolite biosynthesis, energy metabolism, homeostasis maintenance and other physiological functions were ameliorated.
研究领域:
微生物学
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食品科学
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肠道健康
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
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IF 4.1
Qiong Wu,Yanan He,Chunxiao Cui,Xiaoya Tao,Dongdong Zhang,Yurong Zhang,Tiejin Ying,Li Li
BACKGROUND To determine how abscisic acid (ABA) affects tomato fruit ripening at the protein level, mature green cherry tomato fruit were treated with ABA, nordihydroguaiaretic acid (NDGA) or sterile water (control, CK). The proteomes of treated fruit were analyzed and quantified using tandem mass tags (TMTs) at 7 days after treatment, and the gene transcription abundances of differently expressed proteins (DEPs) were validated with quantitative real-time polymerase chain reaction. RESULTS Postharvest tomato fruit underwent faster color transformation and ripening than the CK when treated with ABA. In total, 6310 proteins were identified among the CK and treatment groups, of which 5359 were quantified. Using a change threshold of 1.2 or 0.83 times, 1081 DEPs were identified. Among them, 127 were upregulated and 127 were downregulated in the ABA versus CK comparison group. According to KEGG and protein–protein interaction network analyses, the ABA-regulated DEPs were primarily concentrated in the photosynthesis system and sugar metabolism pathways, and 102 DEPs associated with phytohormones biosynthesis and signal transduction, pigment synthesis and metabolism, cell wall metabolism, photosynthesis, redox reactions, allergens and defense responses were identified in the ABA versus CK and NDGA versus CK comparison groups. CONCLUSION ABA affects tomato fruit ripening at the protein level to some extent. The results of this study provided comprehensive insights and data for further research on the regulatory mechanism of ABA in tomato fruit ripening. © 2023 Society of Chemical Industry.
研究领域:
植物生理学
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蛋白质组学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
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IF 5.399
Xiaoman Yang,Shuang Lin,Yongxia Jia,Fazal Rehman,Shaohua Zeng,Ying Wang
The Lycium ruthenicum (Lr) fruit is a widely used nutritional food that contains various bioactive components such as anthocyanin and spermidine derivatives. In the present study, ultra-high-performance liquid chromatography with tandem mass spectrometry was utilized to profile the metabolic dynamics of four developmental stages of Lr fruit. A total of 49 compounds, including anthocyanin, alkaloids, hydroxycinnamic acid derivatives, flavonoids, and amino acids, were tentatively identified. Principal component analysis distinguished the fruit at four developmental stages using 15 (9 were tentatively identified) potential marker compounds. Pearson correlation analysis suggested that anthocyanin and spermidine derivative hexoses had a strong positive correlation coefficient. A glucosyltransferase (HG27071) was confirmed to glucosylate both anthocyanidin and spermidine derivative in vitro . Our results provide insight into the metabolic linkages among bioactive components in Lr fruits. The glucosyltransferase identified in this study will promote its potential use in functional foods and natural pigment resources.
研究领域:
植物科学
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生物化学
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食品科学
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DOI
PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 8
·1397
·
IF 6
Jinchen Han,Yanlin Song,Xiaping Fu
Conventional spatial frequency domain imaging (SFDI) based optical property inversion is inefficient, while deep learning methods suffer from heavy reliance on large-scale real datasets. To address this contradiction, a simulation-driven approach for subsurface fruit bruise discrimination was proposed. An SFDI simulation environment was built with Blender to generate 800 paired datasets of diffuse reflectance images and optical transport coefficients, overcoming the high cost and long cycle of real dataset acquisition. We designed the CBAM-GAN-U-Net model and adopted surface profile correction in the prediction method to eliminate curved surface-induced non-planar distortion, with the whole method validated on liquid phantoms, green apples and crown pears. This prediction method achieved high accuracy in predicting the reduced scattering coefficientμs′, with NMAE of 0.021 ± 0.007 (phantoms), 0.039 ± 0.012 (severely bruised green apples) and 0.044 ± 0.015 (severely bruised crown pears), outperforming U-Net and GANPOP. Based on the predictedμs′, a discrimination strategy combining coefficient of variation, mean ratio and receiver operating characteristic (ROC) curve analysis was adopted, attaining 100% accuracy for non-bruised/bruised fruit discrimination, with misclassification rates of 6% (green apples) and 8% (crown pears) for mild/severe bruise differentiation. This method enables accurate subsurface fruit bruise detection, providing a reliable technical solution for the fruit and vegetable industry and helping reduce postharvest supply chain losses.
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Jialiang Liu,Tongtong Liang,Yue Xiang,Benfeng Zhang,Lisha Zhu,Xiangbin Xu,Xuequn Shi,Zhengke Zhang
Starch degradation is a fundamental process underlying the development of key postharvest quality attributes in ripening mango fruit; however, its regulatory mechanisms remain incompletely understood. In this study, ethephon (ETH) and 1-methylcyclopropene (1-MCP) were used as ripening modulators to investigate the patterns of starch degradation and its transcriptional regulation in postharvest mango fruit. The results demonstrated that ETH fumigation accelerated the ripening process, while 1-MCP treatment effectively inhibited this process. Transcriptome and RT-qPCR analyses revealed and validated that six starch-degrading genes (MiAMY1.1, MiAMY3, MiBAM3, MiBAM9, MiGWD and MiPWD) were regulated in response to ETH. Furthermore, the transcription factor MiRAP2.11 was identified as a nucleus-localized transcriptional activator of MiBAM3 and MiPWD. Transient transformation assays demonstrated that MiRAP2.11 positively regulated starch degradation, thereby promoting development of ripening and quality attributes. The results indicate that MiRAP2.11 functions as a crucial regulator mediating ethylene-induced starch degradation during mango ripening.
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PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 12
·5451-5464
·
IF 5.396
Zu-Man Dou,Chun Chen,Qiang Huang,Xiong Fu
A novel heteropolysaccharide fraction (BBP-24-3) with a relative molecular weight of 145.1 kDa was isolated from blackberry fruits. The BBP-24-3 was mainly composed of arabinose, glucose, and galacturonic acid with a ratio of 5.30 : 3.60 : 91.10 mol%. Structural analysis showed that BBP-24-3 possessed a 1,6-linked β-D-Glcp, 1, 2, 3, 5 linked α-L-Araf, and 1, 4 linked α-D-GalpA backbone with branches substituted at the C-2 and C-5 positions of arabinose units, which included 1, 2, 3, 4 linked β-D-Glcp and T-linked β-D-GalpA. The conformation analysis indicated that BBP-24-3 exhibited a solid spherical structure with a uniform distribution in 0.1 M NaCl solution. The BBP-24-3 exhibited excellent α-glucosidase inhibitory activity with an IC50 value of 3.70 mg mL−1, which was due to the structural change, including α-helix and random coil of α-glucosidase caused by BBP-24-3. The current work suggests the potential utilization of BBP-24-3 as an α-glucosidase inhibitor in healthy food for reducing the postprandial blood glucose level.
研究领域:
食品科学
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药理学
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生物化学
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DOI
PubMed
高影响因子
Frontiers in Chemistry
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2021
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IF 5.221
Chen Kaiwei,Du Linlin,Gao Peng,Zheng Junli,Liu Yuanli,Lin Hua
The carboxylate-functionalized passion fruit peel (PFPCS) was an efficient and rapid biosorbent for wastewater treatment. The PFPCS exhibited excellent selectivity to the cationic dyes, where the maximum adsorption capacities for methylene blue (MB) and methyl violet (MV) were 1,775.76 mg g−1 and 3,756.33 mg g−1, respectively. And the adsorption process of MB and MV on PFPCS reached equilibrium within 20 min. Moreover, the adsorption conditions and mechanisms were investigated. The adsorption process was in good agreement with the pseudo-second-order and Langmuir isotherm models. The adsorption mechanism was also proposed to be electrostatic interaction and hydrogen bond. After six cycles of desorption-adsorption, the removal efficient of MB and MV could be kept above 95%. Thus, PFPCS was considered as a highly efficient absorbent for removing cationic dyes from polluted water due to excellent adsorption characteristics, low cost and environmental friendliness.
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）