Materials Today Bio
·
2026
·
IF 11
Yuhan Hu,Qifan Zhou,Haitong Wen,Tianyi Huang,Yongqing Liu,Ye Liu,Xin Jin,Xiaoyu Lu,Hangyi Wu
Recently, copper (Cu)-based cancer therapy, an intracellular Cu-dependent process, has received tremendous attention because of the emergence of cuproptosis. However, the inherent regulatory mechanism limits intracellular Cu accumulation. Herein, a carrier-free GSH-responsive in situ nanoreactor (TECJ) is fabricated, of which Cu ionophore elesclomol (ES), GSH-responsive nitric oxide (NO) donor O 2 -(2,4-dinitrophenyl) 1-[(4-ethoxycarbonyl) piperazin-1-yl] diazen-1-ium-1,2-diolate (JSK) and Cu 2+ are integrated via self-assembly and coated with D-α-tocopherol polyethylene glycol 1000 succinate (TPGS). TECJ enhances the systemic stability and tumor accumulation of ES and JSK through both the coating function and the additional inner force provided by TPGS. After internalization, TECJ specifically releases the cargoes via GSH-responsive dissociation. Then, ES efficiently transports extracellular Cu 2+ into the cytoplasm for Cu influx, while NO released from JSK aggravates mitochondrial dysfunction and blocks adenosine triphosphate supply to inhibit ATP7A expression and reduce Cu efflux, therefore dually resulting in Cu-overload and amplifying cuproptosis. Furthermore, Cu-induced Fenton-like reaction together with TPGS-mediated ROS generation triggers chemodynamic therapy (CDT), and NO transfers ROS into more toxic ONOO − for accelerating tumor death while reducing tumor self-alleviation by inhibiting DNA repair. Afterwards, the process above synergistically activates immunogenic cell death and cascades immunotherapy. Finally, TECJ successfully suppresses tumor growth and prevents tumor metastasis.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhexiang Wang,Xinyi Li,Rui Lv,Ruochen Guo,Qinglei Shu,Tianjin Ge,Jun Yan,Zhihao Zhao,Guanghui Wang,Jian Liu
The click chemistry catalyzed by transition metals is the research front line for bioorthogonal therapeutics. However, it demands the use of external ligands to form transition metal–ligand complexes for the catalytic enhancement, thus leading to biosafety concerns on the undesired side effects of the complexes or the additional ligands themselves during therapies. Here we report a pH/GSH-driven inside-out nanoreactor initiating Cu(I)-precursor chelation-promoted bioorthogonal catalysis against malignant tumors. It comparts the precursors (Azide and Alkyne) and Cu(II) safely with disulfide-functionalized hyaluronic acid-polycarprolactone (abbr. PCHP1) polymersome structure. Once sensing the pH/GSH changes typically in tumor microenvironment, our nanoreactor starts with a unique programed releasing, allowing for the inside-out translocation of Azide with the highest priority. Subsequently the Cu(I)-Azide chelation can promote the catalytic reaction to generate drug molecules in a highly efficient, ligand-free manner. The PCHP1 nanoreactor can suppress malignant tumors significantly better than the other control groups in the in vitro and in vivo experiments, while inducing minimal side effects. Our strategy of ligand-free Cu(I)-precursor chelation promoted click chemistry will facilitate the development of bioorthogonal catalytic therapies for clinical translation.
研究领域:
药学
·
肿瘤学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Xinggong Hu,Jiong Li,Guoyong Du,Chunping Deng,Sike Chen
With the development of oil enterprise and petrochemical industry, more and more oily wastewater is generated, which highlights the urgency of oil/water separation. In order to solve the troubles of cumbersome membrane training and effortless clogging when the use of the membrane separation method, this find out about proposes a new and simple technique to prepare modified oil/water separation membranes based on commonly used PVDF membranes with the assist of covalent cross-linking of pyrogallic gallic acid (PG) and glutathione (GSH), and chelating and liganding of copper nanoparticles (Cu NPs). PG can be rapidly oxidized by Michael addition/Schiff response to generate a mussel-like adhesion layer, and the hydrophilic coating formed by way of covalent cross-linking with glutathione (GSH) incorporates a giant variety of catechol functional groups, which can be chelated and ligated with Cu NPs to structure a steady superhydrophilic phenolic community coating. The Cu@MF-PG/GSH membranes were capable to separate more than a few kinds of stable oil-in-water emulsions with surfactants, and the separation fluxes were giant (all fluxes have been >779 L/m2.h). Meanwhile, the separation efficiencies were all greater than 99% with excellent biking stability (flux recovery >97%). In addition, the self-cleaning property of Cu@MF-PG/GSH membranes was tested. The outcomes showed that the Cu@MF-PG/GSH membrane could be restored to a easy kingdom after simple water rinsing even when it was once contaminated by soybean oil with high viscosity. It suggests terrific self-cleaning performance
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Hanyu Yao,Ruixuan Ji,Xiaoyi Song,Bin Yang,Yin Lv,Zhong Wei
Ferroptosis, a recently recognized form of non-classical programmed cell death (PCD), leads to impaired cellular structure and integrity due to the accumulation of lipid peroxidation products (LPO). However, single treatment ferroptosis often fails to achieve optimal tumor treatment outcome. Therefore, we constructed a multi-synergistic treatment strategy based on ferroptosis, that is more conducive to tumor therapy. In this study, we constructed a metal organic framework (MOF) loaded with glucose oxidase (GOx) and doxorubicin (DOX), denoted as Fe(Ⅲ)-RSSR@DOX@PEG-GOx. Fe(Ⅲ)-RSSR@DOX@PEG-GOx was synthesized through coordination between Fe3+ and disulfide-containing ligands scavenged GSH and downregulate glutathione peroxidase 4 (GPX4), thereby triggering ferroptosis. The GOx catalyzes glucose into abundant H2O2, promoting the Fenton reaction which results in excessive ROS formation within tumors. The accumulation of ROS can further enhance the process of ferroptosis, while the massive consumption of glucose constitutes a synergistic situation of “starvation treatment”. The smart biomimetic nanoplatform integrates ferroptosis, chemotherapy, and starvation therapy, providing a potential anti-tumor strategy to achieving multi-effect synergistic tumor treatment.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Fanpeng Kong,Ziye Liang,Dongrui Luan,Xiaojun Liu,Kehua Xu,Bo Tang
To reduce the side effects of chemotherapy, nontoxic prodrugs activated by the tumor microenvironment are urgently required for use in cancer treatment. In this work, we developed prodrug 4 for tumor-targeting treatment and imaging of the anticancer drug release in vivo. Taking advantage of the high glutathione (GSH) concentration in cancer cells, the disulfide bond in prodrug 4 was cleaved, resulting in the release of an active anticancer drug and a near-infrared (NIR) fluorescence dye turn-on. Furthermore, contrast to the free anticancer drug, the prodrug exhibited higher cytotoxicity to hepatoma cells than that to normal HL-7702 cells. Thus, prodrug 4 is a promising platform for specific tumor-activatable drug delivery system, because of its favorable features of in situ and in vivo monitoring of the drug release and therapeutic efficacy.
研究领域:
肿瘤学
·
药物化学
·
分子影像学
✓ 已验证
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2022
·
IF 6.819
Yang Liping,He Jian,Tao Zhenchao,Zhou Yan,Yang Jing,Zhang Yangyang,Gao Jing,Qian Liting
Cancer poses a serious threat to human health and is the most common cause of human death. Polymer-based nanomedicines are presently used to enhance the treatment effectiveness and decrease the systemic toxicity of chemotherapeutic agents. However, the disadvantage of currently polymeric carriers is without therapy procedure. Herein, for the first time, glutathione (GSH)-responsive polymer (PRES) with anti-cancer effect was synthesized following the condensation–polymerization method using resveratrol (RES) and 3,3′-dithiodipropionic acid. PRES can not only suppress the tumor cells growth but can also self-assemble into nanoparticles (∼93 nm) for delivering antitumor drugs, such as paclitaxel (PTX@PRES NPs). The system could achieve high drug loading (∼7%) and overcome multidrug resistance (MDR). The results from the in vitro studies revealed that the NPs formed of PRES were stable in the systemic circulation, while could be efficiently degraded in tumor cells high GSH environment. Results from cytotoxicity tests confirmed that PTX@PRES NPs could effectively suppress the growth of cancer cells (A549) and drug-resistant cells (A549/PTX). The NPs could also be used to significantly increase the therapeutic efficacy of the drugs in A549/PTX tumor-bearing mice. In vivo investigations also demonstrated that the PRES-based NPs exhibited tumor inhibition effects. In summary, we report that the GSH-responsive polymer synthesized by us exhibited multiple interesting functions and could be used for effective drug delivery. The polymer exhibited good therapeutic effects and could be used to overcome MDR. Thus, the synthesized system can be used to develop a new strategy for treating cancer.
研究领域:
药学
·
纳米技术
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 84
·80896-80904
·
IF 3.289
Hongliang Cao,Huajie Song,Debiao Xie,Chao Chen,Xin Chen,Ping Wang,Wenxin Wang
We report a glutathione (GSH) responsive drug carrier that is based on the Michael addition reaction between an olefinic bond and GSH. Amphiphilic copolymers with multi-linear-cyclized and branched structures that contain vinyl groups were successfully synthesized by deactivation enhanced atom transfer radical polymerization (DE-ATRP). The polymers self-assembled into GSH responsive micelles with sizes of 68 to 97 nm and spherical morphology. The core cross-linked micelles were confirmed to greatly decrease doxorubicin (DOX) leakage due to the concentration gradient. Drug release experiments indicated that the drug carrier containing no disulfide has the same responsive ability as a conventional GSH reduction responsive carrier with a similar polymer structure. MTT assays showed that the blank micelles are totally non-cytotoxic, while the DOX-loaded micelles exhibit GSH-dependent behaviour.
研究领域:
药剂学
·
生物材料
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 49
·27740-27745
·
IF 2.936
Yibo Wang,Bin Sun,Bin Han,Min Hu
Malignant melanoma is a highly lethal disease, and advanced stages of melanoma have proven to be resistant to many chemotherapeutic drugs. Cancer stem cells (CSCs) and high levels of intracellular glutathione (GSH) have been proven to play important roles in drug resistance. Retinoic acid (RA) is a promising anticancer agent, which can inhibit proliferation and induce differentiation of CSCs, but its clinical use has been limited by its water insolubility and weak cancer cell killing effect when used alone. Herein, by combining RA and ferrocene, a new type of derivative of retinoic acid (FCRA) was synthesized and then oxidized by FeCl3. The oxidized FCRA (FCRA+) was exploited as a novel anticancer agent. Compared with RA, FCRA+ not only has improved water solubility and stronger anti-cancer effect to melanoma cells through depleting intracellular GSH of the cancer cells, but also can inhibit proliferation and induce differentiation of melanoma CSCs, such as free RA. Therefore, FCRA+ has better application prospects than RA and may replace RA for clinical applications.
研究领域:
肿瘤学
·
药物化学
·
癌症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Colloid and Interface Science Communications
·
2023
·
IF 4.5
Chengyang Ban,Hanzhu Shi,Qiong Ding,Bin Wu,Ru Xia,Jiasheng Qian,Ming Cao
In this study, Fe/ABTS/PVP nanocomplexes (named FAPs) as photothermal-reinforced catalytic antibacterial agents were prepared using the coordination-induced assembly of Fe (III), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and polyvinylpyrrolidone (PVP). The redox pairs of Fe(II)/Fe(III) in FAPs can catalyze the H 2 O 2 to hydroxyl radicals (•OH) through Fenton reactions, thereby killing effect on bacteria. The photothermal effect of ABTS •+ in FAPs stimulated by near infrared (NIR) irradiation can further improve the catalytic effect, thereby generating more •OH and achieving better antibacterial activity. What's more, the reduction reaction between glutathione (GSH) and ABTS •+ results in the depletion of the GSH level, the redox pair of Fe(II)/Fe(III) is also beneficial in the consumption of GSH. This dual depletion of GSH further expands the intracellular oxidative stress. Thus, the integrated function of photothermal effect, catalytic property, and dual depletion of GSH by FAPs provides a new strategy for fighting against bacterial infections.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
抗菌研究
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Yanning Zheng,Dapeng Xu,Lixia Sun,Junrong Ji,Jianhua Sun,Zhangfa Tong,Lingyi Qin,Youquan Zhang,Jiangxue Luo,Dankui Liao
The catalytic synergy of different nanomaterials is a potential technique used to develop a highly active and stable nanozyme . Here, magnetite on nitrogen-doped hollow carbon sphere (Fe 3 O 4 /N-HCS) composites were prepared through solvent thermal method and exhibited excellent oxidase and peroxidase-like catalytic activity under different pH levels. The dual enzyme-like catalyzed reaction of Fe 3 O 4 /N-HCS conformed to the typical Michaelis–Menten kinetics, and Fe 3 O 4 /N-HCS had a strong affinity for substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with H 2 O 2 . The remarkable performance of the composites was achieved by the synergistic effect of Fe 3 O 4 and N-doped activated graphite carbon, which accelerated the formation of O 2 •- and •OH during the dual enzyme-like catalytic reaction . Furthermore, an effective colorimetric glutathione (GSH) sensor with a wide linear range from 0.02 µM to 250 µM and a detection limit as low as 9.3 nM was constructed based on the superior peroxidase-like activity of Fe 3 O 4 /N-HCS. The convenient colorimetric sensor monitored the content of GSH in the serum of mice, and the detection results were satisfactory, with a recovery of 96–103% and an RSD between 0.80% and 3.30%. This study discussed the internal mechanism of Fe 3 O 4 /N-HCS nanozyme and developed an efficient method for GSH detection to expand nanozyme application in bioanalysis .
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Yongjuan Li,Ningjing Lei,Xiaohan Yao,Yu Zhang,Ya Dong,Jinmeng Yi,Xinyan Li,Wenjing Deng,Guangjun Nie,Zhihai Qin
The clinical application of ferroptosis, characterized by iron-dependent lipid peroxidation, is limited because of the serious side effects of using toxic-dose iron. Herein, a polyvalent ferroptosis agonist-a hypoxia responsive polymer bearing 18-crown-6 ring (hPPAA18C6) is developed. In contrast to the natural ferroptosis agonists (erastin, RSL3, and sorafenib), hPPAA18C6 stimulates the ferroptosis by releasing endogenous iron stored in the natural “iron pools” of cellular organelles and depleting glutathione (GSH) via the benzoquinone generated from the cascade decaging reactions in hypoxia. hPPAA18C6 nanoparticle is loaded with photosensitizer-chlorine e6 (Ce6) (hPPAA18C6@Ce6) due to the inhomogeneous hypoxia microenvironment. Moreover, the exposed positively charged primary amine (NH 2 ) from hPPAA18C6 acts as an immune adjuvant, facilitating dendritic cells maturation, antigen presentation, and cytotoxic T lymphocyte activation. hPPAA18C6@Ce6 induces anti-tumor responses that are dependent on ferroptosis, photodynamics therapy (PDT), and CD8 + T-cell activity. In addition, the combination of hPPAA18C6 and Ce6 leads to combined therapeutic outcomes in primary, distant, and metastatic tumors. The activation of the ferroptosis pathway through functional polymer-hijacking endogenous iron and GSH may offer new therapeutic opportunities.
研究领域:
肿瘤学
·
免疫治疗
·
分子生物学
·
聚合物化学
✓ 已验证
DOI
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2023
·Vol. 40
·期 9
·2300041
·
IF 2.7
Wenhui Bao,Jun Ai
In this work, silver nanoparticles are synthesized using a simple and sensitive method by using double-stranded DNA (dsDNA-Ag NPs) as a template. The prepared dsDNA-Ag NPs are characterized by fluorescence spectroscopy analysis, X-ray photoelectron spectroscopy analysis, and transmission electron microscopy analysis. The excitation wavelength of the prepared silver nanoparticles is 295 nm, the emission wavelength is 377 nm, the average particle size is 11.2 nm, and the dispersion is uniform with pleasurable stability. The nanomaterials are used as fluorescent probes to detect glutathione (GSH). After adding glutathione to the dsDNA-Ag NPs fluorescent probes, the fluorescence of dsDNA-Ag NPs is burst due to electron transfer and S Ag bond generation, and the linear range of detection concentration is 0–90 m m with a detection limit of 0.37 m m .
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）