ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yaping Li,Yongqi Yang,Chunmeng Zhong,Dan Xiao,Cuisong Zhou
Sensitive detection of resistance mutation T790 M is of great significance for early diagnosis and prognostic monitoring of non-small-cell lung cancer (NSCLC). In this paper, we showed a highly sensitive detection strategy for T790 M using a three-level characteristic current signal pattern in an α-hemolysin nanopore. A probe was designed that formed a C–T mismatched base pair with wild-type/P and a T–T mismatched with the T790M/P. The T790M/P produced a unique three-level characteristic current signal in the presence of mercury ions(II): first, T790M–Hg2+–P entering the vestibule of α-HL under the transmembrane potential and overhang of probe occupying the β-barrel, then probe unzipping from the T790M/P, T790 M temporally residing inside the nanocavity due to the interaction with Hg(II), and finally T790 M passing through the β-barrel. The blocking current distribution was concentrated with a small relative standard deviation of about 3%, and the signal peaks of T790 M and wild-type can be completely separated with a high separation resolution of more than 2.5, which achieved the highly sensitive detection of T790 M down to 0.001 pM (confidence level P 95%) with a linear range from 0.001 pM to 1 nM in human serum samples. This highly sensitive recognition strategy enables the detection of low abundance T790 M and provides a method for prognostic monitoring in NSCLC patients.
研究领域:
生物物理学
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医学诊断
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分子生物学
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DOI
PubMed
高影响因子
Nanotechnology Reviews
·
2022
·Vol. 11
·期 1
·2038-2049
·
IF 6.739
Haiyun Fan,Pengying Jia,Hailei Zhang,Zhiyi Su,Xinwu Ba,Yonggang Wu
The development of a facile approach to simultaneously detect and separate Hg( ii ) ions in an aqueous solution is a challenging topic in the chemosensing field. Herein, we focus on constructing the Hg( ii )-sensitive fluorescence “turn-on”-type composite materials by using halloysite nanotube (HNT) as substrate. Two types of HNTs-based chemosensors, HNTs-PHT and HNTs-BP, were developed in this study, which exhibit Hg( ii )-sensitive fluorescence “turn on” behavior by forming interparticle and intraparticle excimers, respectively. Fortunately, HNTs-PHT is able to effectively restrict the solvent relaxation of π–π* transition and make it a better detection tool in aqueous solution than HNTs-BP. The addition of Hg( ii ) can trigger a dramatical increase at 469 nm in emission curves of HNTs-PHT, which cannot exhibit emission behaviors without the addition of Hg( ii ). Furthermore, the formation of interparticle excimers makes Hg( ii ) serve as a crosslinker to aggregate HNTs-PHT into precipitations. Following this way, Hg( ii ) ions can be facilely removed from the water via a simple filtration or centrifugation approach. The as-prepared HNTs-PHT shows high specificity and precision in simultaneously detecting and separating Hg( ii ) without the recourse to energy consumption, which will give a novel insight to deal with heavy metal pollution.
研究领域:
化学传感
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环境科学
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材料科学
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DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2026
·
IF 4.5
Bing Liu,Yanshu Xie,Hongli Wang,Xiaohan Tang,Yuan Gao,Fanqing Meng,Shuo Wang
Thiourea-modified magnetic litchi-shell biochar was successfully synthesized. • MTBC-700 had good adsorption performance for As(V) and Hg(II). • The removal rate of As(V) in scallop or rice was 90.64% or 76.59%. • The removal rate of Hg(II) in scallop or rice was 57.51% or 95.51%. A novel litchi-shell magnetic biochar modified by thiourea was successfully synthesized and been applied for the adsorption of the hazardous heavy metals in rice and scallop. It exhibited a high specific surface area and excellent pore volume by nitrogen adsorption-desorption experiment. Under optimal conditions, the adsorption performance and mechanism of heavy metals were separately investigated. The adsorption isotherms fitted the Langmuir model well, and the adsorption process was indicated as a spontaneous endothermic reaction by Gibbs free energy change (ΔG). The removal efficiencies of As(V), Hg(II), Pb(II), Cd(II), and Cr(VI) in scallop were 90.64%, 57.51%, 25.75%, 64.11%, and 39.60%, respectively. While the removal efficiencies in rice were 76.59%, 95.51%, 31.25%, 1.04%, and 25.18% in turn. The adsorbent possesses the advantages of simple preparation, easy separation, and excellent adsorption performance. It is an effective and applicable adsorbent for removing hazardous heavy metals in food. Download: Download high-res image (412KB) Download: Download full-size image
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高影响因子
Analytical Methods
·
2019
·Vol. 12
·期 4
·491-498
·
IF 2.378
Zhang-Jin He,Tian-Fang Kang,Li-Ping Lu,Shui-Yuan Cheng
In this study, a novel electrochemiluminescence (ECL) biosensor based on a CdSe@CdS quantum dot (QD)-functionalized MoS2-modified electrode was developed for the sensitive detection of mercury ions. Polycationic poly(diallyldimethylammonium chloride) (PDDA) and negatively charged QDs were adsorbed on the surface of MoS2, in sequence, to form the QD–PDDA–MoS2 composites, which were employed as matrices for immobilizing thymine-rich DNA sequences (DNA1). The gold nanoparticles (AuNPs) were combined with another type of thymine-rich DNA sequences (DNA2) and glucose oxidase (GOD) to prepare the GOD–AuNP–DNA2 conjugates. The composites were characterized via UV-visible absorption spectroscopy, fluorescence and transmission electron microscopy. A thymine–Hg2+–thymine complex was formed through Hg2+ mismatching with DNA1 and DNA2. GOD was, therefore, modified onto the electrode surface. GOD catalyzed the reduction of dissolved oxygen by glucose to produce hydrogen peroxide, which was a co-reactant of the QDs. The electrochemiluminescence signal of the biosensor increased linearly with the increase in the Hg2+ concentration over the range from 1.0 × 10−12 to 1.0 × 10−6 M with a detection limit of 1 × 10−13 M. The reproducibility, stability and specificity of the biosensor was also studied. The biosensor was used to determine Hg2+ in a real water sample and satisfactory results were obtained.
研究领域:
分析化学
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环境科学
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生物传感技术
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DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2017
·Vol. 29
·期 3
·1030-1038
·
IF 1.907
Gonglei Shao,Junfeng Xiao,Zhihong Tian,Jiajia Huang,Siguo Yuan
A novel chelating resin (PPS-ATD) containing N and S elements was prepared through the chloromethylation of polyphenylene sulfide resin and subsequent functionalization with 2-amino-1,3,4-thiadiazole (ATD). The structure of PPS-ATD was systematically characterized and analyzed by the Fourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, scanning electron microscope, and energy dispersive spectrometer. The adsorption performance of PPS-ATD was evaluated by batch methods at different pH, temperature, adsorption time, and initial concentration, and the results showed that the PPS-ATD exhibited high adsorption capacity (197.79 mg·g −1 at 298 K) and selectivity for Hg(II). The adsorption of PPS-ATD for Hg(II) was well fitted by the Langmuir isotherm model, and the adsorption process was endothermic. After 5 consecutive regeneration cycles, no obvious loss in the adsorption capacity of the PPS-ATD was found, which implied that the PPS-ATD had great application prospects in the treatment of mercury-containing wastewater.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Junjun Fu,Jiayi Zhu,Yan Tian,Kui He,Huang Yu,Linlin Chen,Dongjun Fang,Dongmei Jia,Jinyi Xie,Hao Liu,Jiasheng Wang,Fangcheng Tang,Jinsong Tao,Jinbin Liu
Mercury(II) pollution is a growing threat to the environment and humanity. But most of the solution-based Au nanomaterial sensors suffer from low stability seriously. Here, inspired by high-affinity metallophilic Hg 2+ –Au(I) interaction mechanism, we introduced a transparent cellulose nanofiber matrix-supported luminescent gold nanoparticle (AuNPs) stable sensing membrane. In this solid-state membrane, the AuNPs with strong red fluorescence emission were synthesized as the sensor unit. Cellulose nanofibrilatted (CNF) matrix was fabricated as the supporting substrate. With a large specific surface area, the nanostructured CNF matrix offers abundant immobilizing spots to the sensor unit of AuNPs. More importantly, the excited fluorescence can escape directly without apparent scattering loss due to the high transparency of the CNF substrate. With strong emission of the AuNPs, coupled with abundant AuNP immobilizing spots and high transparency of the CNF substrate, the fluorescence signaling path was manipulated sequentially from signal generation, transmission until to quenching, resulting in the membrane demonstrates high sensitivity, selectivity, and stability. The detection limit of 1.0 nM is markedly lower than the threshold level of 10.0 nM permitted by US EPA. Aided with the high-performance membrane, the detection of Hg(II) becomes simple, fast, easy handing, and green. We envision the membrane will be an attractive material for Hg(II) monitor.
研究领域:
环境科学
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纳米材料科学
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分析化学
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DOI
高影响因子
LUMINESCENCE
·
2020
·Vol. 35
·期 8
·1296-1303
·
IF 1.855
Hai-Bo Wang,An-Li Mao,Yong-Hong Li,Tian Gan,Yan-Ming Liu
Fluorescent adenine (A)-rich DNA-templated gold nanoclusters were demonstrated to be a novel probe for determination of biothiols (including cysteine, glutathione, and homocysteine). Fluorescence intensity of adenine-rich DNA-templated gold nanoclusters could be greatly quenched by Hg(II) ions through the formation of a gold nanoclusters–Hg(II) system. When biothiols (cysteine as the model) were introduced into the system, the fluorescence intensity recovered due to the formation of a more stable Hg(II)-thiol coordination complex using Hg-S metal–ligand bonds, which inhibited the Hg(II)-mediated fluorescence quenching of adenine-rich DNA-templated gold nanoclusters. Based on this fluorescence phenomenon, an on–off–on fluorescence strategy was designed for the sensitive determination of biothiols. The method allowed sensitive detection of cysteine with a linear detection range from 100 nM to 5 μM and a limit of detection of 30 nM. Additionally, the assay can be applied for detection of biothiol levels in human plasma samples. Therefore, it can provide a simple and rapid fluorescent platform for biothiol detection.
研究领域:
分析化学
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生物化学
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纳米技术
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生物传感
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 2
·649-659
·
IF 3.637
Halawa Mohamed Ibrahim,Wu GuoXing,Li Bing Shi
In this work, we fabricated gold nanoparticles (AuNPs) capped with both polyvinyl pyrrolidone (PVP) and iodine (I 2 ) to act as efficient chemiluminescent coreactants for luminol. AuNPs synthesis was based on the direct chemical reduction of Au 3+ with NaBH 4 in the presence of PVP-I 2 complex. The successful synthesis of PVP-I 2 @AuNPs was confirmed with scanning electron microscopy (SEM) and UV-vis spectrophotometry. Chemiluminescence (CL) intensity of luminol was greatly enhanced, upon its chemical reaction with chemisorbed I 2 on AuNPs surfaces owing to the excellent catalytic activity of AuNPs. The PVP-I 2 @AuNPs/luminol CL sensing system was successfully applied for determination of Hg 2+ ions and the results displayed linearity in a wide range from 0.5 to 2000 nM and an ultrasensitive response to 1.0 nM Hg 2+ . The detection limit of Hg 2+ ions was 0.1 nM, which was 100 times lower than the limit value (10 nM) defined by the U.S. Environmental Protection Agency in drinkable water. This ultrasensitive luminogenic system for Hg 2+ detection also exhibited excellent selectivity among 13 types of metals, suggesting that the luminol/PVP-I 2 @AuNPs system is a promising sensor for real-time detection of Hg 2+ .
Graphical abstract
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Zhiying Shao,Jilai Lu,Jian Ding,Fengjiao Fan,Xinyang Sun,Peng Li,Yong Fang,Qiuhui Hu
Chitosan-pectin gel beads (CPBs) were synthesized via a facile and green method and applied to remove heavy metals from aqueous solution. The structural characteristics of CPBs were investigated by SEM and FTIR, the mechanical strength of CPBs was measured by Texture Analyzer and the stability of CPBs was evaluated in acidic solution. To study the adsorption characteristics, the effect of pH, contact time, initial heavy metals concentration, temperature, adsorption mechanism and regeneration were systematically investigated. The adsorption kinetics fitted well pseudo-second-order model, and the adsorption isotherms were well described by Langmuir model. The maximum adsorption capacities of Cu(II), Cd(II), Hg(II) and Pb(II) were 169.4, 177.6, 208.5 and 266.5 mg/g, respectively. The adsorption-desorption experiments revealed that the CPBs exhibited a great reusability. Thus, the synthesized CPBs in this study had the potential to be utilized as an environment-friendly and green adsorbent for the removal of heavy metals.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3513-3519
·
IF 4.58
Huang Yicun,Yan Fanyong,Xu Jinxia,Bian Yuyao,Zhang Ruiqi,Wang Jie,Zhou Xuguang
The authors describe the synthesis of a two-dimensional organic-inorganic perovskite of the type NH 3 (CH 2 ) 10 NH 3 )PbBr 4 by a fast precipitation method. The resulting powder under photoecitation at 270 nm exhibits yellow luminescence (peaking at 550 nm) in DMSO/water mixtures if the ratio is bigger than 4/6. On addition of Hg(II) and a rhodamine spirolactam, ring opening occurs and colorimetric and fluorescent responses can be seen. Specifically, the emission at 550 nm gradually decreases and a new emission peak appears at 585 nm (corresponding to the emission peak of RBEA-Hg(II) complex) while the color of the solution gradually turns to pink under visible light. The emission peak of the modified perovskite overlaps the 585 nm absorption of rhodamine B, and this results in a FRET effect whose Förster radius (donor-acceptor distance) is calculated from decay time data to be 10.4 Å. Based on these findings, a FRET-based assay for Hg(II) was worked that has a 2.4 μM detection limit.
In DMSO/H 2 O = 1/9 mixed solution, energy transfer from NH 3 (CH 2 ) 10 NH 3 )PbBr 4 (C 10 PbBrPE) to rhodamine B occurred. Fluorescence resonance energy transfer (FRET) process is available by rhodamine B ethylenediamine and C 10 PbBrPE for detection of Hg(II).
研究领域:
材料科学
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分析化学
·
环境科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Jian Zhu,Tian-Tian Jia,Jian-Jun Li,Xin Li,Jun-Wu Zhao
In this work, we develop a simple and selective sensing method for the detection of mercury ions based on surface plasmon resonance (SPR) spectrum change of Au-Ag core-shell triangular nanoplates . When the concentration of mercury is increased, the etching-induced change of particle size and shape also leads to the decrease of the absorption peak at the fixed wavelength, until a spectrum dip takes place. This spectral change of “peak-to-dip” greatly enlarges the detection range of mercury ions, which could be fine tuned by changing the initial thickness of the Ag coating. Under optimal conditions, the decrease of the logarithmic absorption intensity has a good linear response with the concentration of mercury ions increasing from 10 to 1000 μM, and the limit of detection (LOD) is 0.88 μM. Interference studies and real samples test indicate that, this new sensing method has a good selection for mercury ions and can be practically used in lake water. This work shows the surface etching-induced SPR shift can also leads to the intensity change with “peak-to-dip” fashion, which greatly enlarge the concentration range of the detection and could be widely applied in the spectroscopy sensing based on SPR.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2016
·Vol. 32
·期 2
·231-239
·
IF 1.452
Lu Lai,Si-Yu Sheng,Ping Mei,Yi Liu,Qing-Lian Guo
High-quality Cd x Zn 1 – x Se and Cd x Zn 1 – x Se/ZnS core/shell quantum dots (QDs) emitting in the violet–green spectral range have been successfully prepared using hydrothermal methods. The obtained aqueous Cd x Zn 1 – x Se and Cd x Zn 1 – x Se/ZnS QDs exhibit a tunable photoluminescence (PL) emission (from 433.5 nm to 501.2 nm) and a favorable narrow photoluminescence bandwidth [full width at half maximum (FWHM): 30–42 nm]. After coating with a ZnS shell, the quantum yield increases from 40.2% to 48.1%. These Cd x Zn 1 – x Se and Cd x Zn 1 – x Se/ZnS QDs were characterized by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared (FTIR) spectroscopy. To further understand the alloying mechanism, the growth kinetics of Cd x Zn 1 – x Se were investigated through measuring the fluorescence spectra and X-ray diffraction spectra at different growth intervals. The results demonstrate that the inverted ZnSe/CdSe core/shell structure is formed initially after the injection of Cd 2 + . With further heating, the core/shell structured ZnSe/CdSe is transformed into alloyed Cd x Zn 1 – x Se QDs with the diffusion of Cd 2 + into ZnSe matrices. With increasing the reaction temperature from 100 °C to 180 °C, the duration time of the alloying process decreases from 210 min to 20 min. In addition, the cytotoxicity of Cd x Zn 1 – x Se and Cd x Zn 1 – x Se/ZnS QDs were investigated. The results indicate that the as-prepared Cd x Zn 1 – x Se/ZnS QDs have low cytotoxicity, which makes them a promising probe for cell imaging. Finally, the as-prepared Cd x Zn 1 – x Se/ZnS QDs were utilized to ultrasensitively and selectively detect Hg 2 + ions with a low detection limit (1.8 nM).
研究领域:
材料科学
·
分析化学
·
生物医学工程
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PubMed
高影响因子
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