RSC Advances
·
2019
·Vol. 9
·期 58
·33589-33595
·
IF 3.049
Yun Zhang,Shuyou Shi,Jiajia Xing,Wenqing Tan,Chenguang Zhang,Lin Zhang,Huan Yuan,Miaomiao Zhang,Jinjuan Qiao
In this study, a novel colorimetric sensing platform was developed for the detection of S. aureus using dog immunoglobulin G (IgG) as the capture antibody and chicken anti-protein A immunoglobulin Y labeled with horseradish peroxidase (HRP-IgY) as the detection antibody. Dog IgG labeled with magnetic beads was used to capture S. aureus through the interaction between the Fc region of dog IgG and Staphylococcal protein A (SPA). HRP-IgY was introduced to recognize the residual SPA on the surface of S. aureus and to create a sandwich format, after which a soluble 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added. A stop solution was utilized to cease the enzymatic chromogenic reaction, and then optical density was read at 450 nm. Under optimal conditions, the proposed method displayed a low detection limit of 1.0 × 103 CFU mL−1 and a wide linear range of 3.1 × 103 to 2.0 × 105 CFU mL−1. This detection method exhibited high specificity against other foodborne bacteria. The recovery rates ranged from 95.2% to 129.2%. To our knowledge, this is the first report to employ dog IgG and chicken IgY as an antibody pair to detect S. aureus. This technique exhibits high application potential for S. aureus monitoring in various kinds of samples.
研究领域:
生物传感器
·
微生物学
·
食品安全
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 3
·855-862
·
IF 3.719
Yan Fufeng,Zhang Yuanchang,Zhang Shuai,Zhao Jihong,Liu Shunli,He Linghao,Feng Xiaozhong,Zhang Hongzhong,Zhang Zhihong
We report on an efficient method for the production carboxy-modified graphene (carboxy-GR), and its application to a highly sensitive immunoassay for the livestock feed additive clenbuterol. Octadecanoic acid is shown to self-assemble on carboxy-GR via intermolecular interaction. Goat anti-mouse IgG was conjugated to 1-octadecanethiol-modified carboxy-GR and then deposited by self-assembly on a C18-modified gold surface. The resulting sensor surface was used to quantify clenbuterol by both surface plasmon resonance and electrochemical impedance spectroscopy. The assay works in the 0.01 ng∙mL −1 to 10 ng∙mL −1 concentration range and the lower detection limit is 6.57 pg∙mL −1 . It is concluded that this fairly simple method can be extended to immunoassays for other food additives.
Principle of sensor system and immunoassay. (i) Binding of ACG–IgG with the alkyl chain of 1-octadecanethiol through intermolecular force onto the gold substrate; (ii) absorbance of Ab CLB onto the surface of ACG-IgG; and (iii) detection of CLB using an immunoassay based on Ab CLB –ACG–IgG.
研究领域:
材料科学
·
分析化学
·
食品安全
·
生物化学
·
纳米技术
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DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Wenliang Ge,Steven Suryoprabowo,Xiaoling Wu,Qiankun Zheng,Hua Kuang
The misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-reactivity in pig urine. For the immunochromatographic test, the coating antigen which was obtained by conjugating the salbutamol to bovine serum albumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the control line and test line. The test was developed with a sensitive monoclonal antibody specific for MAB, which was generated by immunizing BALB/c mice with a well-characterized MAB- keyhole limpet haemocyanin conjugate, produced in our laboratory. Under optimized conditions, the cut-off limits of the semi-quantitative test strip for MBA and MPA were as low as 10 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. Our data indicate that the immunochromatographic assay allows provide sensitive, rapid, and specific on-site screening for MAB and MAP.
研究领域:
兽医学
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分析化学
·
公共卫生
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DOI
高影响因子
Nano Today
·
2024
·
IF 13.2
Keying Chen,Chunxiong Zheng,Yunjuan Lv,Pengkai Zhao,Tong Lin,Yanteng Xu,Huimin Kong,Ke Yi,Qingguo Zhong,Mingqiang Li,Yu Tao,Haixia Wang
In acute inflammation, the heightened activation and recruitment of immune cells present an opportunity to leverage them as natural carriers for efficient transport of diagnostic probes and nanotherapeutics. Although complement opsonization facilitates the internalization of nanomedicines by activated neutrophils, the development of strategies to specifically augment complement deposition remains a challenge. Herein, we engineer silymarin-loaded liposomes (Lips) coated with immunoglobulin G (IgG) to enhance complement 3 (C3) deposition in the protein corona, thereby enabling neutrophil-mediated, precise targeting to the site of inflammation. Through the examination of various serum proteins, we discover that IgG adsorption, particularly with its Fc portion exposed, prominently promotes C3 enrichment in the protein corona, resulting in C3 cleavage into iC3b. This facilitates the uptake of C3-enriched Lips by activated neutrophils with elevated C3 receptor expression, thus improving the efficiency and specificity of nanomedicine delivery to inflammatory site. Following the formation of neutrophil extracellular traps, the released nanomedicine effectively mitigates hepatocyte damage by eliminating accumulated reactive oxygen species and inducing a shift in macrophage polarization towards the anti-inflammatory M2 phenotype. Our IgG-modified nanomedicine demonstrates significant therapeutic efficacy against acute liver failure by regulating the protein corona and hitchhiking neutrophils, offering a promising strategy for efficient and precise treatment of inflammation.
研究领域:
药理学
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免疫学
·
纳米技术
·
肝病学
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiangfei Hu,Hui Wang,Yueming Hu,Pingwei Wen,Xiongchen Wu,Zongcai Tu
The effect of electric field intensities (EFIs, 5–20 kV/cm) and treatment times (0.5–2 h) on allergenicity and spatial conformation of prawn tropomyosin was evaluated. The results demonstrated that the IgG and IgE binding capacity of tropomyosin maximally increased by 24.34 % and 29.16 % respectively, followed by a subsequent decrease after 20 kV/cm treatment for 1 h. Interestingly, 5–10 kV/cm treatments significantly decreased the α-helix content ( P < 0.05) and fluorescence intensity, while 20 kV/cm treatment promoted extensive spiralization, resulting in a tightly packed structure. The increased flexibility further exposed the hydrolysis sites and strengthened the gastrointestinal digestibility of tropomyosin. Additionally, molecular dynamic simulation indicated that extended EFIs increased structural flexibility and depolymerized the tropomyosin dimers through destroying intermolecular hydrogen bonds (formed within arginine and glutamate), which allowed tropomyosin to be easily recognized by IgG/IgE. Whereas, decrease of solvent-accessibility surface area (SASA), hydrophobic surface area induced conformation folded and caused epitopes masked.
研究领域:
食品科学
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过敏研究
·
生物物理
·
分子生物学
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 12
·2901-2907
·
IF 3.864
Wenyue Xie,Lingli Lei,Meiling Tian,Zeying Zhang,Yingshuai Liu
A high-resolution colorimetric immunoassay platform has been developed based on enzyme-catalyzed multicolor generation and smartphone-assisted signal readout. The multi-color generation is accomplished in this system through the urease-catalyzed urea hydrolysis-induced color change of the pH indicator phenol red, from yellow to orange to red over pH 6.6 to 8.0. The color change is easily tailored by controlling the urease activity via the inhibitor silver ion (Ag+), the amount of which is in turn adjusted by alkaline phosphatase (ALP)-catalyzed ascorbic acid (AA) production. An ALP-linked colorimetric immunoassay is readily realized based on the above urease catalyzed multicolor generation system. Under optimal conditions, a limit of detection (LOD) of 1.73 ng mL−1 and a dynamic range from 0 to 18 ng mL−1 are achieved with rabbit IgG as a model analyte. A colored picture for each test is directly taken using a smartphone and then quantitatively analyzed with the free software ImageJ, eliminating the use of expensive and desktop equipment. The dose-dependent multicolor display is easier to distinguish with the naked eye for qualitative or semiquantitative detection over the traditional one-color system. The developed immunoassay scheme provides a promising platform for on-site testing or applications in resource-poor areas.
研究领域:
分析化学
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生物传感
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即时检测
·
移动健康
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-6
·
IF 5.228
Tang Zhongxue,Ma Zhanfang
In this work, we presented a ratiometric electrochemical immunosensor based on redox substrate and immunoprobe. Carboxymethyl cellulose-Au-Pb 2+ (CMC-Au-Pb 2+ ) and carbon-Au-Cu 2+ (C-Au-Cu 2+ ) nanocomposites were firstly synthesized and implemented as redox substrate and immunoprobe with strong current signals at −0.45 V and 0.15 V, respectively. Human immunoglobulin G (IgG) was used as a model analyte to examine the analytical performance of the proposed method. The current signals of CMC-Au-Pb 2+ (I substrate ) and C-Au-Cu 2+ (I probe ) were monitored. The effect of redox substrate and immunoprobe behaved as a better linear relationship between I probe /I substrate and Lg C IgG (ng mL −1 ). By measuring the signal ratio I probe /I substrate , the sandwich immunosensor for IgG exhibited a wide linear range from 1 fg mL −1 to 100 ng mL −1 , which was two orders of magnitude higher than other previous works. The limit of detection reached 0.26 fg mL −1 . Furthermore, for human serum samples, the results from this method were consistent with those of the enzyme linked immunosorbent assay (ELISA), demonstrating that the proposed immunoassay was of great potential in clinical diagnosis.
研究领域:
分析化学
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生物传感器
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电化学
·
免疫学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Ziyu Han,Yanyan Wang,Xuexin Duan
This paper reports a surface functionalization strategy for protein detections based on biotin-derivatized poly( l -lysine)-grafted oligo-ethylene glycol (PLL- g -OEG x -Biotin) copolymers . Such strategy can be used to attach the biomolecule receptors in a reproducible way simply by incubation of the transducer element in a solution containing such copolymers which largely facilitated the sensor functionalization at an industrial scale. As the synthesized copolymers are cationic in physiology pH, surface biotinylation can be easily achieved via electrostatic adsorption on negatively charged sensor surface. Biotinylated receptors can be subsequently attached through well-defined biotin-streptavidin interaction. In this work, the bioactive sensor surfaces were applied for mouse IgG and prostate specific antigen (PSA) detections using quartz crystal microbalance (QCM), optical sensor (BioLayer Interferometry) and conventional ELISA test (colorimetry). A limit of detection (LOD) of 0.5 nM was achieved for PSA detections both in HEPES buffer and serum dilutions in ELISA tests. The synthesized PLL- g -OEG x -Biotin copolymers with different OEG chain length were also compared for their biosensing performance. Moreover, the surface regeneration was achieved by pH stimulation to remove the copolymers and the bonded analytes , while maintaining the sensor reusability as well. Thus, the developed PLL- g -OEG x -Biotin surface assembling strategy is believed to be a versatile surface coating method for protein detections with multi-sensor compatibility.
研究领域:
生物技术
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生物传感器
·
分析化学
·
材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Zhen Song,Rong Li,Xiqin Yang,Zhanhua Zhang,Xiliang Luo
Electrochemical biosensors with high sensitivity and cost-effectiveness, are considered as a promising alternative to existing clinical diagnostic techniques, but their practical assaying application in complex biological samples was significantly limited by the severe biofouling. Herein, an electrochemical biosensing platform with enhanced antifouling abilities was constructed based on a designed DNA-peptide conjugate, which was synthesized with an anchoring DNA and a difunctional (antifouling and recognizing) peptide. The DNA-peptide conjugates were easily immobilized onto an electrode, which was modified with poly(3,4-ethylenedioxythiophene) (PEDOT) and gold nanoparticles (AuNPs), through the interaction between the anchoring DNA and AuNPs, and the recognizing peptide was specific for the binding of target human immunoglobulin G (IgG). As both the anchoring DNA and the difunctional peptide possessed antifouling abilities, the IgG biosensor based on the DNA-peptide conjugates exhibited enhanced antifouling properties, and it was capable of detecting IgG directly in real human serum, with a wide linear range from 0.1 ng·mL −1 -10 μg·mL −1 and a low limit of detection (0.037 ng·mL −1 ). The strategy of developing biosensors with functional DNA-peptide conjugates as antifouling materials offered an effective and simple way to achieve low fouling protein detection in complex human serum.
研究领域:
生物化学
·
分析化学
·
生物医学工程
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电化学
·
生物传感器
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DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-8
·
IF 5.7
Yu Yao,Li Yansong,Xu Ning,Zha Yonghong,Bai Xue,Liu Mingyuan,Zhou Yu,Liu Xiaolei
Fumonisin B1 (FB1) is one of the important mycotoxins posing health risks in the area of food safety. A sensitive fluorescence ratio immunoassay has been established for FB1 based on the growth of monodispersed 2-D MnO 2 nanosheet on an individual gold nanoparticle (AuNP@MnO 2 ). FB1 competed with the coated FB1-BSA to bind the FB1 monoclonal antibody. After a washing step, alkaline phosphatase-labeled goat anti-mouse IgG (ALP-IgG) with high catalytic activity was combined with FB1 monoclonal antibody. ALP reacts with ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA), which decomposes AuNP@MnO 2 to dehydroascorbic acid (DHAA). O-Phenylenediamine dihydrochloride (OPD) is oxidized to yellow-fluorescent substrate of 2,3-diaminophenazine (DAP) (excitation, 423 nm; emission, 570 nm) by AuNP@MnO 2 . Meanwhile, OPD can also be reduced to blue fluorescent substrate of OPDred (excitation, 350 nm; emission, 430 nm) by DHAA. The content of FB1 can be determined by fluorescence ratio of blue/yellow. The limit of detection (LOD) of the fluorescence ratio immunoassay for FB1 was 0.06 ng mL −1 , and the linear range was from 0.25 to 60.00 ng mL −1 . The effectiveness of the assay was verified in real maize samples, and satisfactory recoveries were attained. The correlation coefficient of these results between the fluorescence ratio immunoassay and commercial ELISA kit was 0.9999. This method provides a sensitive and selective tool for the detection of FB1 in maize samples. Graphical Abstract
研究领域:
食品安全
·
分析化学
·
纳米技术
·
毒理学
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DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 5
·1024-1031
·
IF 4.2
Xueyi Zhu,Ning Ma,Yizhen Wan,Lu Wang,Yu Zhang,Chengyu Yan,Weiping Qian
Immobilizing ligands is a crucial part of preparing optical sensors and directly connected to the sensitivity, stability, and other characteristics of sensors. In this work, an ordered porous layer interferometry (OPLI) system that can monitor the covalent coupling process of ligands in real time was developed. Films of silica colloidal crystal (SCC), as optical interference substrates, were surface modified by three different reagents: chloroacetic acid, glutaric anhydride, and carboxymethyl dextran. Staphylococcus aureus protein A (SPA), the ligand, was immobilized on SCC films. The covalent coupling process of SPA and SCC films can be dynamically monitored by the OPLI system. In addition, the three different strategies were evaluated by comparing the efficiency of the sensors prepared by different methods for binding Immunoglobulin G (IgG). The glutaric anhydride-modified sensor offers apparent advantages in terms of bound IgG quantity and affinity. This system provides a simple and intuitive way to determine the efficiency of different covalent coupling strategies. Furthermore, the sensor covalently coupled with SPA also excels in the determination of IgG content in complex systems such as milk. At the same time, the covalent coupling gives the sensor the ability to be stored stably over time.
研究领域:
材料科学
·
分析化学
·
光学传感
·
表面化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2024
·
IF 2.6
Zhou Miao,Wu Zichen,Deng Donghua,Wang Bin,Zhou Xiaoling,Zhou Bingyu,Wang Chunping,Zeng Yan
Oxidative damage resulting from weaning stress significantly impacts the growth performance and health status of piglets. Taurine, a dietary antioxidant with diverse functions, was investigated in this study for its protective role against weaning stress-induced oxidative damage and its underlying mechanism. Forty 28-day-old male castrated weaned piglets were randomly assigned to four groups. The control group received the basal diet, while the experimental groups were fed the basal diet supplemented with 0.1, 0.2%, or 0.3% taurine over a 28-day period. In vitro, H2O2 was utilized to induce oxidative damage to the jejunal mucosa of piglets via IPEC-J2 cells. The results demonstrated that taurine supplementation reduced the incidence of diarrhea in piglets compared to that in the control group (p < 0.05); the addition of 0.2 and 0.3% taurine led to increased average daily gain and improved feed conversion efficiency in weaned piglets, showing a linear dose-response correlation (p < 0.05). Taurine supplementation at 0.2 and 0.3% enhanced the activities of serum CAT and GSH-Px while decreasing the levels of serum NO, XOD, GSSG, and MDA (p < 0.05). Moreover, it significantly elevated the levels of GSS, Trx, POD, complex I, mt-nd5, and mt-nd6, enhancing superoxide anion scavenging capacity and the hydroxyl-free scavenging rate in the livers of weaned piglets while reducing NO levels in the liver (p < 0.05). Additionally, 0.2 and 0.3% taurine supplementation decreased serum IL-6 levels and elevated the concentrations of IgA, IgG, and IL-10 in weaned piglets (p < 0.05). The levels of occludin, claudin, and ZO-1 in the jejunum mucosa of weaned piglets increased with 0.2 and 0.3% taurine supplementation (p < 0.05). In IPEC-J2 cells, pretreatment with 25 mM taurine for 24 h enhanced the activities of SOD and CAT; reduced the MDA content; upregulated the mRNA expression of various genes, including ZO-1, occludin, claudin-1, Nrf2, and HO-1; and reversed the oxidative damage induced by H2O2 exposure (p < 0.05). Overall, the findings suggest that the inclusion of 2 and 3% taurine in the diet can enhance growth performance, reduce diarrhea rates, ameliorate oxidative stress and inflammation, and promote intestinal barrier function in weaned piglets.
研究领域:
动物科学
·
营养学
·
生理学
·
兽医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）