Food Bioscience
·
2025
·
IF 5.9
Xiaowei Chen,Yuhao Huang,Junping Liu,Kaibo Yu,Yangyang Li,Wenjing Tan,Linfeng Yuan,Shengfeng Peng,Lei Zhou,Wei Liu
The preservation of lily bulbs has attracted attention due to the reduction of shelf life caused by high humidity. In this study, a moisture-responsive carboxymethyl absorbent paper embedded with HKUST-1 (HKUST-1@CMAP) was successfully prepared by in-situ growth method. Characteristic peaks and crystallization peaks of HKUST-1 were observed in the FTIR spectra and XRD spectra of HKUST-1@CMAP, respectively. The loading capacity of clove essential oil (CEO) in composite paper was 4.62%. Essential oil composite paper (CEO-HKUST-1@CMAP) exhibited moisture-responsive release properties, with the release of clove essential oil increasing from 34.99% to 84.38% within 18 days as the moisture increased from 20% to 60%. Meanwhile, CEO-HKUST-1@CMAP showed excellent antimicrobial capacity, antioxidant property and biocompatibility. During storage, essential oil composite paper effectively delayed the browning of lily bulbs, maintained their firmness, and inhibited the growth of microorganisms, thereby maintaining the overall storage quality of lily bulbs stored at 25 °C for 16 days. In addition, CEO-HKUST-1@CMAP successfully improved cell membrane integrity by enhancing the activities of SOD and APX, inhibiting the activity of POD, and reducing the accumulation of MDA. In conclusion, CEO-HKUST-1@CMAP effectively prolonged the shelf life of lily bulbs, indicating that it is a promising preservative for the preservation of lily bulbs.
研究领域:
材料科学
·
食品科学
·
植物学
·
生物技术
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Guoqiang Guan,Houkang Cao,Zixuan Tang,Kefeng Zhang,Mingli Zhong,Rui Lv,Weimin Wan,Fengyue Guo,Yongwang Wang,Ya Gao
Ethnopharmacological relevance Polygala fallax Hemsl. is a traditional folk medicine commonly used by ethnic minorities in the Guangxi Zhuang Autonomous Region, and has a traditional application in the treatment of liver disease. Polygala fallax Hemsl . polysaccharides (PFPs) are of interest for their potential health benefits. Aim of this study This study explored the impact of PFPs on a mouse model of cholestatic liver injury (CLI) induced by alpha-naphthyl isothiocyanate (ANIT), as well as the potential mechanisms. Materials and methods A mouse CLI model was constructed using ANIT (80 mg/kg) and intervened with different doses of PFPs or ursodeoxycholic acid. Their serum biochemical indices, hepatic oxidative stress indices, and hepatic pathological characteristics were investigated. Then RNA sequencing was performed on liver tissues to identify differentially expressed genes and signaling pathways and to elucidate the mechanism of liver protection by PFPs. Finally, Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were used to verify the differentially expressed genes. Results Data analyses showed that PFPs reduced the levels of liver function-related biochemical indices, such as ALT, AST, AKP, TBA, DBIL, and TBIL. PFPs up-regulated the activities of SOD and GSH, down-regulated the contents of MDA, inhibited the release of IL-1β, IL-6, and TNF-α, or promoted IL-10. Pathologic characterization of the liver revealed that PFPs reduced hepatocyte apoptosis or necrosis. The RNA sequencing indicated that the genes with differential expression were primarily enriched for the biosynthesis of primary bile acids, secretion or transportation of bile, the reactive oxygen species in chemical carcinogenesis, and the NF-kappa B signaling pathway. In addition, the results of qRT-PCR and Western blotting analysis were consistent with those of RNA sequencing analysis. Conclusions In summary, this study showed that PFPs improved intrahepatic cholestasis and alleviated liver damage through the modulation of primary bile acid production, Control of protein expression related to bile secretion or transportation, decrease in inflammatory reactions, and inhibition of oxidative pressure. As a result, PFPs might offer a hopeful ethnic dietary approach for managing intrahepatic cholestasis.
研究领域:
药理学
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分子生物学
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肝病学
·
民族药理学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Yidan Zhang,Hui Lian,Linhui Yang,Lingling Tong,Yang Wu,Suxing Jin,Dongsheng Guo
Ophiocordyceps gracilis is a type of fungus commonly utilized in both health food and Chinese medicine. Herein, we prepared antioxidant hydrolysate from O. gracilis , and isolated and purified the prepared hydrolysate to obtain O. gracilis peptides-4-3-1 (OGP-4-3-1) grade with the highest scavenging activity on DPPH, ABTS and hydroxyl radicals. Three new peptides with high antioxidant capacity were identified as LFNHF, PLDRHPF and YPFLRPL, respectively. The three peptides can reduce the proportion of senescent cells conveying positive expression of senescence-associated-β-galactosidase (SA-β-Gal) and increase survival pace of H 2 O 2 -induced human hepatocellular carcinomas (HepG2 cells), and the protective effects on cellular against senescence might be related to their increase in the activities of antioxidant enzymes, as well as the decrease in the concentration of malondialdehyde (MDA) and reactive oxygen species (ROS). The molecular docking results indicated that they could form durable hydrophobic and hydrogen bonds with critical amino acid residues of the Kelch-like ECH-associated protein 1 (Keap1), which could potentially modulate pathway of nuclear factor erythroid 2-related factor 2 (Nrf2) in conjunction with Keap1. In conclusion, the current research offers a theoretical foundation for the further development of the anti-aging effect of OGP, which has a broad application prospect in the fields of functional food and cosmetics.
研究领域:
生物化学
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药理学
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细胞生物学
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食品科学
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化妆品学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Yuexing Zhao,Weitao Liu,Jinzheng Liu,Ruiying Shi,Aurang Zeb,Xiang Li,Yichen Ge
N -(1,3-dimethylbutyl)- N′ -phenyl- p -phenylenediamine (6PPD), a widely utilized antioxidant in automobile tires and rubber goods, is frequently detected in aquatic ecosystems and poses a potential threat to aquatic organisms. However, research on the impact of 6PPD on aquatic plants is still scarce. Here, we investigated the bioaccumulation of 6PPD in Myriophyllum verticillatum ( M. verticillatum ) (watermilfoil), and its impacts on biochemical characteristics and metabolomics. 6PPD (10,100 mg/L) significantly inhibited the growth and photosynthetic pigment content of M. verticillatum . After 14 days of exposure to 100 μg/L 6PPD, accumulation levels of 6PPD and its metabolite 6PPDQ in M. verticillatum reached 0.52 mg/kg and 0.09 mg/kg, respectively. Moreover, 6PPD significantly induced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzymes and glutathione (GSH), reducing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA), thereby mitigating oxidative damage in M. verticillatum . Furthermore, metabolic pathway analysis revealed that 6PPD has remarkable effects on amino acid and sugar metabolism. This study provides data support for understanding the toxic effects of 6PPD on aquatic plants and evaluating its potential risks.
研究领域:
环境科学
·
水生植物学
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毒理学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Wei Xia,Haohong Lin,Xinyu Zhou,Yihu Wang,Shixiang Cao,Jiawei Liu,Anming Xu,Weiliang Dong,Min Jiang
Excessive use of polyurethane (PU) polymers has led contributed to serious environmental pollution. The plastic recycling technology using microorganisms and enzymes as catalysts offers a promising green and low-carbon approach for managing plastic waste. However, current methods for screening PU-degrading strains suffer from drawbacks such as being time-consuming and inefficient. Herein, we present a novel approach for screening PU-degrading microorganisms using a quenching fluorescent probe along with the fluorescence-activated droplet sorting (FADS). The FPAP could specifically recognize the 4,4′-methylenedianiline (MDA) derivates released from PU degradation, with fluorescence quenching as a response. Based on the approach, we successfully screen two PU-degrading strains ( Burkholderia sp. W38 and Bacillus sp. C1). After 20 d of cultivation, strain W38 and C1 could degrade 41.58% and 31.45% of polyester-PU film, respectively. Additionally, three metabolites were identified during the degradation of PU monomer (2,4-toluene diamine, 2,4-TDA) and a proposed degradation pathway was established. Consequently, the fluorescence probe integrated with microfluidic droplet systems, demonstrates potential for the development of innovative PU-biocatalysts. Furthermore, the identification of the 2,4-TDA degradation pathway provides valuable insights that can propel advancements in the field of PU biodegradation.
研究领域:
环境科学
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微生物学
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生物技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Xue-Wu Chen,Liu-Qing Gu,Xin-Yu Zeng,Xin-Yuan Sun,Jian-Ming Ouyang
Hyperoxaluria can easily induce calcium oxalate (CaOx) crystals and cause cell damage, thereby increasing the risk of kidney stone formation. In this study, three sulfated Pelvetia siliquosa polysaccharides (PSPs) were obtained by the sulfur trioxide-pyridine method. The antioxidant activity of PSPs and the inhibitory effects of PSPs on CaOx crystallization, cellular oxidative damage, and cellular inflammation were explored in vitro, and PSPs were used to treat hyperoxaluria-induced crystallization model mice in order to validate the stone-preventive effect of PSPs in vivo. PSPs can inhibit CaOx crystal formation, as well as reduce reactive oxygen species (ROS) levels through their own antioxidant properties and up-regulation of antioxidant enzyme (SOD and CAT) expression, which in turn reduces the release of lactate dehydrogenase (LDH) and malondialdehyde (MDA), improves lysosomal integrity, cellular morphology, and cytoskeleton, inhibits the decrease of mitochondrial membrane potential, reduces adhesion protein (CD44 and OPN) expression, alleviates cellular inflammatory factor (IL-6, TNF-α, and IL-1β) levels, and inhibits apoptosis. PSP3, which has the highest degree of sulfation, had the best protection capacity. PSP3 also showed good antistone ability in mice, and it may be a potential drug for kidney stone prevention.
研究领域:
药理学
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生物化学
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肾病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yuehua Shi,Yu Shi,Zhaoyang Wang,Jiahe Zhang,Ran Hao,Gangwan Zhang,Xiaolong Zhao,Leyong Zeng
To achieve safe and efficient tumor therapy, it was a powerful strategy of constructing an intelligent tumor microenvironment (TME)-responsive nanoprobe. In this study, by coating ultrasmall gold nanoparticles (AuNPs) and manganese dioxide (MnO 2 ) on mesoporous prussian blue (MPB), [email protected] @MnO 2 nanoprobe with H 2 O 2 self-generation was developed for magnetic resonance (MR) imaging, photothermal therapy (PTT) and enhanced chemodynamic therapy (CDT). The results indicated that the prepared [email protected] @MnO 2 possessed uniform size and good biocompatibility. Using glucose-responsive self-supplying H 2 O 2 of ultrasmall AuNPs, and glutathione (GSH)-responsive Mn 2+ release from MnO 2 degradation, the accelerated hydroxyl free radicals (·OH) generation efficiency (80.0 % to 90.84 %) and enhanced MR relaxation (1.93 to 9.96 mM −1 ·s −1 ) were achieved. Importantly, the prepared [email protected] @MnO 2 exhibited excellent photothermal performance with a photothermal conversion efficiency of 42.62 %. Moreover, the intracellular H 2 O 2 /·OH generation was demonstrated, and the in vitro CDT/PTT was enhanced, by which the cell viability was reduced to 6.4 %, stronger than 18.8 % of [email protected] 2 . By modeling MDA-MB-435 tumor-bearing nude mice, the combined CDT/PTT of [email protected] @MnO 2 nanoprobe greatly inhibited the tumor growth, and after 15 days, the tumor completely disappeared. This work provided a promising strategy to develop TME-responsive self-supplying H 2 O 2 nanoprobe, achieving MR imaging and enhanced antitumor efficacy.
研究领域:
纳米技术
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生物医学工程
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癌症治疗
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材料科学
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医学成像
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产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Chen Hui,Zheng Huizhen,Li Tiejun,Jiang Qihong,Liu Shulai,Zhou Xuxia,Ding Yuting,Xiang Xingwei
Oyster peptide (OP) has exhibited useful biological activities and can be used in multi-functional foods. OP has been reported to play a significant role in intestinal protection, but its specific mechanism is still not completely understood. The aim of this study was to analyze the potential effect of OP on oxidative damage of mice intestine induced by cyclophosphamide (Cy). The experimental results revealed that intragastric administration of OP significantly increased average bodyweight, improved ileum tissue morphology and villus structure, as well as increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in oxidized mice serum and liver. The content of malondialdehyde (MDA) in the mice serum and liver homogenate was found to be markedly decreased. Moreover, OP significantly increased the relative mRNA expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone oxidoreductase (NQO1) and heme oxidase-1 (HO-1) in ileum. Western-blot results indicated that prior administration of OP significantly up-regulated the Nrf2 production in ileum, and substantially decreased then Keap1 gene expression. In conclusion, intake of OP was found to markedly improve intestinal oxidative stress in vivo, and this effect was primarily mediated through the simulation of antioxidant Nrf2-Keap1 signaling pathway. This study is beneficial to the application of peptide nutrients in the prevention or mitigation of intestinal oxidative damage.
研究领域:
营养学
·
药理学
·
分子生物学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 3
·1-11
·
IF 3.246
Miao Zhiwei,Zhang Lei,Gu Mingjia,Huang Jianyi,Wang Xiaoyu,Yan Jing,Xu Yan,Wang Libing
This study sought to improve the oral bioavailability and enhance the anti-enteritis effect of fraxetin by incorporating it into long circulating liposomes (F-LC-Lipo). The optimal formulation of F-LC-Lipo was obtained via orthogonal design. The particle size, morphology, encapsulation efficiency, stability, and anti-enteritis effect of F-LC-Lipo were evaluated. The particle size of F-LC-Lipo was 166.65 ± 8.75 nm with entrapment efficiency (EE) of 92.18 ± 0.17%. The release rate in different dissolution media (pH 1.2 HCl, DDW, and pH 7.4 PBS) was significantly higher than that of fraxetin solution. Compared with the free fraxetin solution, F-LC-Lipo increased oral bioavailability of fraxetin by 4.43 times (443%). More importantly, F-LC-Lipo could improve the levels of interleukin-1 beta (IL-1β), IL-6, malondialdehyde (MDA), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), prostaglandin E2 (PEG2), and IL-10 in rats with enteritis. Overall, these results suggested that LC-Lipo may serve as a potential carrier for improving the solubility and oral bioavailability of fraxetin as well as improving its enteritis effect.
研究领域:
药剂学
·
药物递送系统
·
炎症性肠病研究
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
Ling Jiang,Cai-Bo Tian,Rui-Han Ye,Nian Shi,Xing-Chao He,Yun-Li Zhao,Xiao-Dong Luo
Drug-induced liver injury caused acute hepatic failure and hepatitis frequently. In this investigation, kakuol and asarinin reduced the levels of serum alanine transaminase (ALT), aspartate transaminase (AST) and malondialdehyde (MDA) dramatically, and ameliorated the pathological damage of liver tissues in APAP-induced mice. Furthermore, both compounds increased the viabilities of APAP-induced L-O2 cells and extracellular glutathione (GSH) levels accompanied significantly by reducing the level of intracellular ROS in vitro . In addition, HSP90AA1/CDK2/mTOR signaling pathway and five target proteins (CDK2, HSP90AA1, HRAS, MMP1, mTOR) were proposed from network pharmacology and molecular docking prediction, and then the up-regulation of protein expression of CDK2, mTOR and down-regulation of HSP90AA1, HRAS, MMP1 by kakuol and asarinin in western blotting supported their mechanism.
研究领域:
药理学
·
分子生物学
·
毒理学
·
肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xuejing Zhang,Shiwei Niu,Gareth R. Williams,Jianrong Wu,Xia Chen,Hong Zheng,Li-Min Zhu
In this study, we report a novel pH and temperature responsive paclitaxel-loaded drug delivery system based on chitosan and di(ethylene glycol) methyl ether methacrylate. This was functionalized with hyaluronic acid to permit active targeting of CD44-overexpressing human breast cancer cells. The resultant HA-CS-g-PDEGMA-PTX nanoparticles (NPs) have small and uniform sizes (˜170 nm), a high drug loading (13.6 ± 1.3%) and high encapsulation efficiency (76.2 ± 8.5%). Cell viability and confocal microscopy experiments demonstrated that the NPs could effectively target and kill MDA-MB-231 human breast cancer cells, but were much less toxic to healthy human umbilical vein endothelial cells. In vivo biodistribution studies in mice showed that the NPs accumulated in the tumor site, while free drug was distributed more widely and rapidly cleared from the body. Histopathological studies revealed that the NPs led to enhanced apoptosis in the tumor site, which resulted in reduced tumor growth. The NPs prepared in this work have great potential for the treatment of breast cancers, and further offer a platform with which to target other cancers.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2025
·
IF 3.1
Wu Xinhua,Wu Lifang,Li Zhaoquan,Tian Wei,Li Tao
Three series of (dimethyl-, dibutyl-, and diphenyl-) new organotin(IV) complexes based on chlorine substituted aryl ligands were synthesized and characterized by UV, 1 H NMR, 13 C NMR, 119 Sn NMR, HRMS, and X-ray crystallography analysis. MTT results showed that chlorine substitution at different positions on the aryl group exhibited different anticancer activities. Among them, 5-chloro substituents > 3,5-dichloro substituents > 3-chloro substituents on the aryl ring of salicylaldehyde on Schiff base ligand. In addition, different substituents on the tin atom also demonstrated extreme differences in anticancer activities, where dibutyltin > diphenyltin > dimethyltin. Interestingly, the complex LTDB2 exhibited excellent anti-proliferative activity against breast cancer MDA-MB-231 cells (superior to cisplatin) and lower toxicity against human embryonic kidney HEK-293 cells in vitro. Flow cytometry showed that LTDB2 not only induced apoptosis, but also induced cell cycle arrest in G2 phase. These findings provide new insights for further research and development of novel organotin(IV) complexes as anticancer drugs. Complex LTDB2 exhibited excellent anti-proliferative activity against breast cancer MDA-MB-231 cells in vitro and was superior to cisplatin. Flow cytometry showed that LTDB2 not only induced apoptosis, but also induced cell cycle arrest in G2 phase.
研究领域:
化学
·
药理学
·
肿瘤学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）