JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Jia Wang,Chen Zhao,Chengyi Hong,Zhengzhong Lin,Zhiyong Huang
This study attempted to fabricate a novel colorimetric method for the quantitative detection of malachite green (MG) residues in fish and water samples. Based on the recognition of the detection target and the enhancement of the peroxidase-like activities of Fe 3 O 4 NPs by MG-aptamer, a colorimetric method was established for MG detection. The detection limit (3 α / κ , n = 9) was 16.7 μg kg –1 , and the detection linear range was 0.06–2.38 μmol L –1 . The recovery rates by standard addition in fish and water ranged from 90.1% to 119% with standard deviations (n = 3) of less than 6.6 %, and was well validated by a HPLC method. The detection procedure could be completed within 1 h. Therefore, the detection method was simple, stable, and reliable, and could be used to detect MG residues rapidly and accurately in fish and aquaculture water.
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高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·229
·
IF 4.034
Qianqian Wang,Xiaofei Qin,Liping Geng,Yan Wang
A label-free electrochemical aptasensor was fabricated to sensitively determine malachite green (MG) based on Au nanoparticles/graphene quantum dots-tungsten disulfide nanosheet composite film modified glassy carbon electrode (AuNPs/GQDs-WS2/GCE). A facial strategy for the self-assembly of graphene quantum dots (GQDs) on tungsten disulfide nanosheets (WS2) was developed to fabricate 0D/2D nanocomposites. As-prepared GQDs-WS2hybrids exhibited significantly enhanced electrocatalytic properties, and were first used as electroactive materials to construct electrochemical aptasensor. The AuNPs/GQDs-WS2/GCE was prepared through depositing Au nanoparticles on the surface of the GQDs-WS2film, which acted as the electrochemical sensing matrix to covalently immobilize the aptamers of MG via the Au–S bond. In this label-free proposal, the aptasensor was applied to detect MG by monitoring voltammetric signal resulted from electrochemical oxidation of the MG captured by the aptamer. Under the optimized conditions, the aptasensor showed a wide linear range from 0.01 to 10 μM for MG detection with a low detection limit of 3.38 nM (S/N = 3). The method was applied to determination of MG in spiked fish samples and gave satisfactory results.Keywords:electrochemical aptasensor;tungsten disulfide nanosheet;graphene quantum dots;Au nanoparticles;malachite green
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Shi Han,Zhang Liang,Yu Guoliang,Liu Yuting,Chen Ligang
A new dual-emission ratiometric fluorescent probe was synthesized and successfully used for the determination of Malachite Green (MG) in fish farming water. The ratiometric fluorescent probe was successfully composited by sol-gel method using C 3 N 4 and CdTe quantum dots as fluorescent materials combined with mesoporous molecularly imprinted polymers. MG quenches the red fluorescence of the CdTe QDs (with excitation/emission wavelengths at 350/680 nm) while the blue fluorescence of C 3 N 4 (with excitation/emission wavelengths at 350/458 nm) remains unchanged. The change of fluorescence color and fluorescence intensity ratio can be successfully used for quantification of malachite green. In addition, the mesoporous structure has a large surface and good adsorption capacity for malachite green. The normalized intensity of fluorescence increases linearly in the 50–1000 ng·mL −1 MG concentration range, and the detection limit is 10 ng·mL −1 . The imprinting factor is 3.2. The nanoprobe was applied to the determination of MG in fish farming water samples. Recoveries and relative standard deviations were 92.5–97.8% and 2.5–6.2%, respectively.
Schematic representation of synthesis of molecularly imprinted mesoporous silica ratiometric fluorescent probes incorporating C 3 N 4 dots and CdTe quantum dots for determination of malachite green.
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Journal of Water Process Engineering
·
2025
·
IF 6.7
Linna Du,Xinjiao Dong,Huan Wu,Gang Li,Jiacheng Zhou,Yiqing Wu,Yanping Xiao,Jing Qin,Xiu Wang
The activated sludge process exhibits limited efficacy in decolorizing triphenylmethane (TPM) dyes like malachite green (MG) in textile wastewater. This study demonstrates that bioaugmentation with Serratia sp. strain WKD significantly enhances MG removal in activated sludge systems. Within 2 h, the combined system (activated sludge + strain WKD) achieved 98.5 ± 0.1 % decolorization efficiency—a 5.3-fold increase over sludge-only controls (18.5 ± 1.7 %). High-throughput sequencing revealed strain WKD's ecological dominance, reducing microbial alpha diversity (8 h) through competitive exclusion. When coupled with carboxymethyl cellulose-modified nano-zero-valent iron (CMC-nZVI), the hybrid system synergistically enriched stress-tolerant degraders ( Serratia sp. and Pseudomonas sp.), thereby accelerating decolorization. Prolonged incubation (24 h) enabled microbial diversity recovery (Shannon and Simpson indices) as detoxification alleviated environmental stress. Functional profiling identified pathway-specific modulation: critical metabolic functions (e.g., carbohydrate/amino acid metabolism) were transiently suppressed, while membrane transport activity remained intact, preserving system functionality. These findings establish strain WKD and CMC-nZVI as a synergistic bioaugmentation strategy, enabling rapid dye removal while maintaining microbial community resilience through dynamic stress-response mechanisms.
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ANALYST
·
2013
·Vol. 138
·期 9
·2592-2597
·
IF 3.969
Shao-Kai Sun,Bei-Bei Wang,Xiu-Ping Yan
Owing to the biological and clinical significance of deoxyribonuclease I (DNase I), it is highly desirable to develop near-infrared (NIR) fluorescent assays for the determination of DNase I activity. Here we report a label-free NIR fluorescent assay for selective determination of DNase I activity based on malachite green (MG)/G-quadruplexes. In the presence of Na+ or K+, single stranded DNA (ssDNA) is able to form a G-quadruplex structure, thus to increase the rigidity of MG structure and result in a remarkable NIR fluorescence. As DNase I is capable of cleaving all types of DNA indiscriminately to release nucleotide products, the G-quadruplexes are cleaved into oligonucleotides in the presence of DNase I. As a result, the rigidity of MG structure is reduced, and the NIR fluorescence of the solution decreases with increase of DNase I activity, providing a useful platform for low-cost, label-free and convenient detection of DNase I activity. Under the optimum conditions, the proposed label-free NIR fluorescent assay gave a detection limit of 1 u mL−1, and a relative standard deviation of 3.2% for eleven replicate detections of 50 u mL−1 DNase I. The proposed assay was applied to the determination of DNase I activity in spiked human urine samples with recoveries from 99.1 to 109.0%.
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MARINE POLLUTION BULLETIN
·
2012
·
IF 2.503
Ziru Lian,Jiangtao Wang
In this paper, a highly selective sample cleanup procedure combining molecular imprinting technique (MIT) and solid-phase extraction (SPE) was developed for the isolation of malachite green in seawater and seafood samples. The molecularly imprinted polymer (MIP) was prepared using malachite green as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The imprinted polymer and non-imprinted polymer were characterized by scanning electron microscope and static adsorption experiments. The MIP showed a high adsorption capacity and was used as selective sorbent for the SPE of malachite green. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of malachite green in seawater and seafood samples was also established. Finally, five samples were determined. The results showed that malachite green concentration in one seawater sample was at 1.30 μg L −1 and the RSD ( n = 3) was 4.15%.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 17
·26019-26035
·
IF 0
Faheem Muhammad,Hassan Muhammad Azher,Mehmood Tariq,Al-Misned Fahad,Niazi Nabeel Khan,Bao Jianguo,Du Jiangkun
This study synthesized a new thiomalic acid–modified rice husk biochar (TMA-BC) as a versatile and eco-friendly sorbent. After undergoing chemical treatments, the mercerized rice husk biochar (NaOH-BC) and TMA-BC samples showed higher BET surface area values of 277.1 m 2 /g and 305.8 m 2 /g, respectively, compared to the pristine biochar (BC) sample, which had a surface area of 234.2 m 2 /g. In batch adsorption experiments, it was found that the highest removal efficiency for malachite green (MG) was achieved with TMA-BC, reaching 96.4%, while NaOH-BC and BC exhibited removal efficiencies of 38.6% and 27.9%, respectively, at pH 8. The engineered TMA-BC exhibited a super adsorption capacity of 104.17 mg/g for MG dye at pH 8.0 and 25 °C with a dosage of 2 g/L. The SEM, TEM, XPS, and FTIR spectroscopy analyses were performed to examine mesoporous features and successful TMA-BC carboxylic and thiol functional groups grafting on biochar. Electrostatic forces, such as π − π interactions, hydrogen bonding, and pore intrusion, were identified as key factors in the sorption of MG dye. As compared to single-solution adsorption experiments, the binary solution experiments performed at optimized dosages of undesired ions, such as humic acid, sodium dodecyl sulfate surfactant, NaCl, and NaSCN, reflected an increase in MG dye removal of 2.8%, 8.7%, 5.4%, and 12.7%, respectively, which was attributed to unique mesoporous features and grafted functional groups of TMA-BC. Furthermore, the TMA-BC showed promising reusability up to three cycles. Our study indicates that mediocre biochar modified with TMA can provide an eco-friendly and cost-effective alternative to commercially accessible adsorbents. Graphical
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Environmental Technology & Innovation
·
2023
·
IF 7.1
Miao Wang,Jie Zhou,Meng Zhang,Sen Li,Lingyun Li,Ge Chen,Xiaodong Huang,Guangyang Liu,Jing Wang,Donghui Xu
The dye pollution in environmental water and food caused by industrial production and illegal addition is a serious problem worldwide, which endangers ecosystem, food safety and human health. To effectively overcome these challenges, a magnetic bioinspired hierarchical porous MOFs (BHP-MOFs) based on ZIF-8, Fe 3 O 4 and three-dimensional graphene was successfully prepared for selective adsorption of Congo red (CR) and Malachite green (MG) from aqueous solutions. The surface morphology, physicochemical structure and composition of BHP-MOFs were characterized by SEM, TEM, FT-IR, XRD, Zeta potential, TGA and VSM analysis. The prepared BHP-MOFs exhibited a large specific surface area (225.522 m 2 /g), high pore volume (0.248 cm 3 /g) and superparamagnetism, which made it an efficient adsorbent for fast adsorption of CR and MG. The adsorption kinetics studies showed that the adsorption mechanism of CR and MG followed the pseudo-second model and the adsorption process fitted well to Langmuir isotherm equation. Thermodynamic experiments have shown that the adsorption process is spontaneous and endothermic. The maximum adsorption capacities of CR and MG were 176.06 and 448.43 mg/g, respectively. Under the optimum conditions, BHP-MOFs were successfully used as magnetic adsorbents for the removal of CR and MG in environmental water and fruit juices, with removal efficiency ranged from 97.09% to 76.00%. The reusability of the BHP-MOFs was repeated with seven cycles and removal efficiency remained at 63.01% and 82.20% for CR and MG, respectively. It is proved that BHP-MOFs composite has excellent adsorption capacity for dyes and has a wide application prospect in practical applications.
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MOLECULES
·
2022
·Vol. 28
·期 1
·310
·
IF 4.927
Yi Zhang,Shaofeng Li,Yurong Gu,Jianying Zhang,Zhenfeng Yue,Liao Ouyang,Fengjuan Zhao
A novel malachite green molecularly imprinted membrane (MG-MIM) with specific selectivity for malachite green (MG) and leucomalachite green (LMG) was prepared using a hydrophobic glass fiber membrane as the polymer substrate, methyl violet as a template analog, 4-vinyl benzoic acid as the functional monomer, and ethyleneglycol dimethacrylate as the crosslinking agent. MG-MIM and non-imprinted membrane (NIM) were structurally characterized using scanning electron microscopy, surface area analyzer, Fourier-transform infrared spectrometer and synchronous thermal analyzer. The results showed that MG-MIM possessed a fluffier surface, porous and looser structure, and had good thermal stability. Adsorption properties of MG-MIM were investigated under optimal conditions, and adsorption equilibrium was reached in 20 min. The saturated adsorption capacities for MG and LMG were 24.25 ng·cm−2and 13.40 ng·cm−2, and the maximum imprinting factors were 2.41 and 3.20, respectively. Issues such as “template leakage” and “embedding” were resolved. The specific recognition ability for the targets was good and the adsorption capacity was stable even after five cycles. The proposed method was successfully applied for the detection of MG and LMG in real samples, and it showed good linear correlation in the range of 0 to 10.0 μg·L−1(R2= 0.9991 and 0.9982), and high detection sensitivity (detection limits of MG and LMG of 0.005 μg/kg and 0.02 μg·kg−1in shrimp, and 0.005 μg/kg and 0.02 μg/kg in fish sample). The recoveries and relative standard deviations were in the range of 76.31–93.26% and 0.73–3.72%, respectively. The proposed method provides a simple, efficient and promising alternative for monitoring MG and LMG in aquatic products.Keywords:malachite green molecularly imprinted membranes;template analogs malachite green;colloidal gold strip;residuals analysis
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2023
·
IF 7.8
Hong Guo,Yaohua Liu,Yuancai Lv,Yifan Liu,Yunzhi Lin,Minghua Liu
A new nitrogen doped sinocalamus oldhami lignin-based activated biochar (denoted as N-BGAC) with a notably high adsorption efficiency was prepared for the removal of malachite green (MG). The properties and structure of N-BGAC were thoroughly characterized, alongside an investigation into the MG adsorption process . The findings revealed that N-BGAC possessed a substantial specific surface area of 2238 m 2 g −1 , nearly double that of the activated biochar lacking nitrogen doping (BGAC). Additionally, N-BGAC exhibited exceptional adsorption efficiency for MG, boasting a maximum adsorption capacity of 1372.5 mg g −1 , marking an enhancement of nearly 2.5 times compared to BGAC. By exploring the adsorption mechanism, it could be found that the adsorption process was a spontaneous exothermic reaction , and mainly controlled by pore-filling effect, electrostatic interactions, hydrogen bonding, hydrophobic interactions and π-π bonding interactions. Crucially, in the regeneration experiments, the removal percentage of MG could still reach 81.5% after four cycles of regeneration, making it promising in the contaminant removal .
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Kuiyu Yi,Lei Zhang
An ingenious nanoscale fluorescent sensor derived from Eu 3+ -postfunctionalized MIL-53 (Al) (Eu 3+ @MIL-53 [Al]) was fabricated though a simple and effective approach. Malachite green (MG) effectively turned off the luminescence of Eu 3+ @MIL-53 (Al) via fluorescence resonance energy transfer (FRET), thus enabling MG sensing. The developed probe exhibited instantaneous reusability after being cleaned with deionized water. The fluorescence intensity, quenching efficiency, and crystal structure of the recoverable sensor after five recycling processes were unchanged compared with those of the original sample. Moreover, the potential mechanism of MG detection was revealed in detail. This work represents the first attempt to determine MG in aquaculture water and products by using metal–organic frameworks (MOFs). The Eu 3+ @MIL-53 (Al) probe proved to be a remarkable fluorescence probe for MG with high selectivity, sensitivity, and excellent regeneration capability. It provides a promising functional platform for the recognition of illegal MG addition to aquaculture water and products.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Juanjuan Luo,Zekai Wang,Yun Li,Changding Wang,Jiefang Sun,Weichun Ye,Xiaolong Wang,Bing Shao
Swab sampling for the trace surface-enhanced Raman scattering (SERS) analysis of an aromatic dye (e.g., malachite green , MG) residue in fruits as well as fingerprints is promising due to its practical utility and efficiency in food security and forensic science. However, the durability and recyclability of the SERS substrates pose an enormous challenge. In this study, a novel flexible film was constructed by embedding a commercial Ag nanowire suspension into a polydimethylsiloxane film (Ag [email protected] ), which simultaneously exhibits SERS activity, durability and recyclability. High SERS performance was still maintained, even against 20 times stretchability, corrosion by HNO 3 , destruction by ultrasonication and a broad working temperature of −20 ℃ to 80 ℃. Excellent recyclability for MG sensing was achieved in ten cycles. Ag [email protected] was successfully used for the quantitative detection of MG in juices of fruits (such as apple and pear) by drop dripping, with a limit of detection of 10 nM and good anti-interference. Meanwhile, the sensitive detection of the MG residue (only 3 ng/cm 2 ) on fruits was enabled by the on-site surface swabbing by recycling for five cycles. Portable detection of the MG residue was further achieved from the 10th fingerprint after successively touching a dye drop onto the functional film combined with a portable Raman spectrometer.
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危险品化学品经营许可证（带存储）