Chemosensors
·
2023
·Vol. 11
·期 7
·386
·
IF 4.2
Meiling Ping,Wenchao Lv,Chen Yang,Qian Chen,Zongwen Wang,Fengfu Fu
It is crucial that simple and high-throughput methods for determining multiple, or groups of, sulfonamides (SAs) be developed since they are widely used in animal husbandry and aquaculture. We developed a paper-based multicolor colorimetric aptasensor to detect 3 SAs: sulfaquinoxaline (SQ), sulfamethoxypyridazine (SMP) and sulfamethoxydiazine (SMD). Using a broad-specificity aptamer as a bioreceptor, we reduced the growth of nicotinamide adenine dinucleotide I (NADH)–ascorbic acid (AA)-mediated gold nanobipyramids (AuNBPs) to generate a multicolor signal. We also used a paper-based analytical device (PAD) system to deposit AuNBPs for a sensitive color signal read out. The aptasensor can detect more color changes corresponding to the concentrations of SQ, SMP and SMD and has higher sensitivity, better specificity and stability. It can also be used to determine SQ, SMP and SDM individually, or collectively, or any two together with a visual detection limit of 0.3–1.0 µM, a spectrometry quantification limit (LOQ) of 0.3–0.5 µM and a spectrometry detection limits (LOD) of 0.09–0.15 µM. The aptasensor was successfully used to determine SQ, SMP and SDM in fish muscle with a recovery of 89–94% and a RSDn= 5) < 8%, making it a promising method for the rapid screening of total SQ, SMP and SDM residue in seafood.Keywords:aptasensor;antibiotics residue;seafood;sulfonamides;visual detection
研究领域:
分析化学
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环境科学
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食品科学
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生物技术
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产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2016
·Vol. 82
·期 2
·
IF 3.823
Hong-Jun Chao,Yan-Fei Chen,Ti Fang,Ying Xu,Wei E. Huang,Ning-Yi Zhou
In addition to growing on p-cresol, Pseudomonas putida NCIMB 9866 is the only reported strain capable of aerobically growing on 2,4-xylenol, which is listed as a priority pollutant by the U.S. Environmental Protection Agency. Several enzymes involved in the oxidation of the para-methyl group, as well as the corresponding genes, have previously been reported. The enzyme catalyzing oxidation of the catabolic intermediate 4-hydroxyisophthalate to the ring cleavage substrate protocatechuate was also purified from strain NCIMB 9866, but its genetic determinant is still unavailable. In this study, the gene hipH, encoding 4-hydroxyisophthalate hydroxylase, from strain NCIMB 9866 was cloned by transposon mutagenesis. Purified recombinant HipH-His6 was found to be a dimer protein with a molecular mass of approximately 110 kDa. HipH-His6 catalyzed the hydroxylation of 4-hydroxyisophthalate to protocatechuate with a specific activity of 1.54 U mg−1 and showed apparent Km values of 11.40 ± 3.05 μM for 4-hydroxyisophthalate with NADPH and 11.23 ± 2.43 μM with NADH and similar Km values for NADPH and NADH (64.31 ± 13.16 and 72.76 ± 12.06 μM, respectively). The identity of protocatechuate generated from 4-hydroxyisophthalate hydroxylation by HipH-His6 has also been confirmed by high-performance liquid chromatography and mass spectrometry. Gene transcriptional analysis, gene knockout, and complementation indicated that hipH is essential for 2,4-xylenol catabolism but not for p-cresol catabolism in this strain. This fills a gap in our understanding of the gene that encodes a critical step in 2,4-xylenol catabolism and also provides another example of biochemical and genetic diversity of microbial catabolism of structurally similar compounds.
研究领域:
微生物学
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环境科学
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生物化学
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遗传学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Yaju Xue,Boxia Guo,Xiuling Ji,Bowen Li,Yuhong Huang
Glycine serves as a crucial element in basic metabolic processes and acts as a precursor in the production of numerous industrial chemicals. Here we developed a streamlined artificial cascade enzyme catalysis pathway for converting CO2 into glycine through improving the CO2 activation and employing direct C–C condensation. After the optimization of enzyme combinations and dose-dependent regulation, the integration of reduced nicotinamide adenine dinucleotide regeneration and ionic liquids were introduced and facilitated the construction of an artificial cascade ionozyme catalysis system. Consequently, the glycine yield reached a remarkable 17.87-fold increase compared to that of the initial reaction system. Additional molecular simulations further demonstrated that the ionozyme possessed enhanced structural stability and substrate affinity, as indicated by an increase in hydrogen bonds and a diminished interference from water molecules in protein–ligand binding. This innovative approach holds great promise for sustainable CO2 bioconversion to glycine and other valuable chemicals.
研究领域:
合成生物学
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生物催化
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二氧化碳利用
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绿色化学
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代谢工程
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产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2024
·Vol. 7
·期 1
·1-12
·
IF 23.2
Zhang Ying,Liang Hao
The utilization of photocatalytic enzyme-coupled systems is considered a promising solution to the solar energy conversion challenge, but it is hampered by its low cofactor regeneration efficiency. Herein, a biomimetic catalytic system was designed inspired by photosynthesis for cofactor regeneration and CO 2 reduction. The Rh complexes were anchored in the core of metal-organic framework UiO67 and chitosan (CS) on the UiO67@Rh surface skillfully helped the in situ growth of CdS. In this core-shell nanoreactor, UiO67@Rh accepted the electrons produced by CdS for cofactor regeneration. CS acted as a bifunctional bio-scaffold to attach CdS and prevent CdS photocorrosion. Besides, CS spatially separated Rh complexes and CdS, preventing deactivation of Rh complexes by CdS and leakage of Rh complexes. Surprisingly, CS improved the photocatalytic activity and photostability of CdS, while other biomacromolecules did not have this function. The photocatalyst regeneration NADH cooperated with formate dehydrogenase (FDH) for CO 2 reduction. The results showed that 0.72 mM formic acid was produced in 5 h (TOF = 0.14 mmol/mg/h), which was triple as much as in the system of UiO67@Rh@CdS and FDH. This cyclic process could be repeated up to 10 time cycles, where long-term stability of the system was observed and the active state of the enzymes was preserved. This study demonstrated a new and promising organometallic photocatalyst-based artificial cofactor regeneration system, paving the path for further research into the bespoke synthesis of fine chemicals and solar fuels from CO 2 utilizing solar energy. Graphical abstract CdS nanoparticles compositing with chitosan and metal-organic frameworks for cofactor regeneration and CO 2 reduction
研究领域:
光催化
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纳米技术
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生物无机化学
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环境化学
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人工光合作用
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二氧化碳转化
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太阳能转化
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DOI
高影响因子
Nature Protocols
·
2018
·Vol. 13
·期 10
·2362-2386
·
IF 12.423
Zou Yejun,Wang Aoxue,Shi Mei,Chen Xianjun,Liu Renmei,Li Ting,Zhang Chenxia,Zhang Zhuo,Zhu Linyong,Ju Zhenyu,Loscalzo Joseph,Yang Yi,Zhao Yuzheng
Cellular oxidation–reduction reactions are mainly regulated by pyridine nucleotides (NADPH/NADP + and NADH/NAD + ), thiols, and reactive oxygen species (ROS) and play central roles in cell metabolism, cellular signaling, and cell-fate decisions. A comprehensive evaluation or multiplex analysis of redox landscapes and dynamics in intact living cells is important for interrogating cell functions in both healthy and disease states; however, until recently, this goal has been limited by the lack of a complete set of redox sensors. We recently reported the development of a series of highly responsive, genetically encoded fluorescent sensors for NADPH that substantially strengthen the existing toolset of genetically encoded sensors for thiols, H 2 O 2 , and NADH redox states. By combining sensors with unique spectral properties and specific subcellular targeting domains, our approach allows simultaneous imaging of up to four different sensors. In this protocol, we first describe strategies for multiplex fluorescence imaging of these sensors in single cells; then we demonstrate how to apply these sensors to study changes in redox landscapes during the cell cycle, after macrophage activation, and in living zebrafish. This approach can be adapted to different genetically encoded fluorescent sensors and various analytical platforms such as fluorescence microscopy, high-content imaging systems, flow cytometry, and microplate readers. A typical preparation of cells or zebrafish expressing different sensors takes 2–3 d; microscopy imaging or flow-cytometry analysis can be performed within 5–60 min.
研究领域:
细胞生物学
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2022
·
IF 7.336
Minling Ding,Huizhe Niu,Nan Zhang,Tongtong Hou,Ping Guan,Xiaoling Hu
Here we report on a facile, rapid, selective, and stable electrochemical sensing platform for nicotinamide adenine dinucleotide (NADH) based on polythionine-methylene blue/electrochemically reduced graphene oxide modified glassy carbon electrode (PTH-MB DES -ERG/GCE). The ERG was directly formed on a glassy carbon electrode (ERG/GCE) through one-step electrochemical reduction of GO in PBS solution (10 mM, pH 7.4). In a PBS electrolyte, respectively, containing 50% (v/v) three different deep eutectic solvents (DES), thionine and methylene blue were polymerized on the surface of ERG/GCE. The electrochemical properties of the polymers polymerized in different electrolytes were investigated. The PTH-MB DES -ERG composite has a higher performance in the electrocatalytic oxidation of NADH than that of PTH-MB DES . The as-prepared PTH-MB DES -ERG/GCE demonstrated excellent direct electrocatalytic oxidation toward NADH, providing a favorable platform for electron transfer from NADH to the electrode. The sensor displayed a wide linear range from 1.52 μM to 3333.33 μM with a limit of detection of 0.51 nM. The interference from the ascorbic acid and uric acid was negligible. The prepared sensor was further tested for the determination of NADH in artificial urine samples, showing the PTH-MB DES -ERG/GCE formed by electropolymerization have promising biomedical applications.
研究领域:
电化学
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生物传感器
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分析化学
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材料科学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2022
·
IF 24.319
Shihao Li,Yuqing Cheng,Yu Chen,Jing Li,Yiying Sun,Jiafu Shi,Zhongyi Jiang
Electron generation, transfer and utilization in photocatalytic reduction reactions jointly govern the solar-to-chemical conversion efficiency. In natural photosynthesis, the ultrathin thylakoid membrane loads numerous pigments and proteins for light harvesting and electron transfer, which also integrates ferredoxin-NADP + reductase bearing molecule hydrides for electron utilization in NADPH regeneration. Inspired by this, herein, conjugated polymer nanolayer coordinated with (Cp*RhCl 2 ) 2 (CPNL-Rh) is developed through a topological and chemical engineering strategy to synergistically intensify electron generation, transfer and utilization for NADH regeneration. Specifically, the triazine moieties well distributed in CPNL-Rh largely harvest visible light to generate electrons. The electrons are rapidly transferred to Rh cocatalyst through CPNL. Rh cocatalyst finally accepts electrons and protons in solution to form molecule hydrides for implementing NADH regeneration. The turnover frequency of CPNL-Rh for NADH regeneration reaches 44.8 h −1 , among the highest value ever reported and ~346% higher than that of bulk CP with free Rh cocatalyst.
研究领域:
材料科学
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化学工程
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光化学
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催化
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DOI
高影响因子
Bioresources and Bioprocessing
·
2022
·Vol. 9
·期 1
·1-9
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IF 4.983
Ma Yue,Li Tianzhen,Tan Zijian,Ma Long,Liu Haifeng,Zhu Leilei
Catalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert glycerol to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed to convert glycerol into 1,3-dihydroxyacetone (DHA) with a yield of 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM glycerol, 2 mM NAD + , 242 U/mL glycerol dehydrogenase-GldA and NADH oxidase-SpNox K184R at 30 °C. Subsequently, NaOH or NaClO 2 catalyzes the formation of lactic acid and glycolic acid from DHA. The high yield of lactic acid (72.3%) and glycolic acid (78.2%) verify the benefit of the chemoenzymatic approaches. Graphical Abstract
研究领域:
化学工程
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生物技术
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催化
·
生化
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DOI
高影响因子
Catalysis Science & Technology
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2024
·
IF 4.4
Jianhua Liu,Jiashu Li,Fangshu Xing,Jian Liu
Developing biological cofactor regeneration strategies is crucial for photo-enzyme coupling systems. Here, crystalline poly(triazine imide) (PTI) containing the cyano group (–CN) is designed for photocatalytic nicotinamide cofactor oxidation regeneration via π–π interaction. The π-conjugation degree and electron delocalization of PTI can be regulated through –CN modification. Such a unique extended π-conjugated system promotes the adsorption of the reduced-state nicotinamide cofactor (NADH) on the aromatic skeleton. Moreover, the enhanced charge transfer dynamics expedite the formation of intermediate active species. Accordingly, the optimized conversion rate of NADH to NAD+ reached 98.2% within 60 min. In contrast, pristine PTI and amorphous polymeric carbon nitride (PCN) gave oxidation yields of 88.1% and 56.8%, respectively. The exclusive selectivity of the oxidized product was further verified by the enzymatic method and nuclear magnetic resonance characterization. This study introduces an innovative method for modulating non-covalent interactions for photo-enzymatic catalysis via the extended π-conjugated system.
研究领域:
材料科学
·
光化学
·
生物催化
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产品关联
DOI
高影响因子
Cell Reports Physical Science
·
2025
·
IF 7.3
Weiqiang Wang,Weifeng Zhang,Fengjiao Chu,Tingting Xiao,Dongdong Hu,Pengyi Tang,Jiajia Gao,Honghua Ge
The release of CO 2 has challenged the worldwide environment and led to climate change. An emerging solution is to construct bio-abiotic semiartificial photocatalysis systems for the highly efficient conversion of CO 2 . However, biocompatibility and the narrow product spectrum are challenging these bio-abiotic photocatalysis systems integrating living cells and semiconductors. Living bacterial biofilms can provide a large specific surface area for photosensitizer mineralization, allowing CO 2 fixation, one-carbon product uptake, and further assimilation. Here, we report a living photosensitizer-mineralized biofilm platform with a biocompatible and stable bio-abiotic interface conducting single-enzyme to whole-cell photocatalytic applications. Such engineered photosensitizer-mineralized biofilms not only efficiently implement the regeneration of NADH but also enable the conversion of CO 2 into formate and finally into polyhydroxyalkanoates under light illumination. Thus, this work demonstrates an alternative approach to solar-to-chemical conversion. The proof of concept established in this work might be used for other sustainable photocatalytic applications.
研究领域:
合成生物学
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环境生物技术
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生物工程
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材料科学
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光催化
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可持续化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 44
·21848-21855
·
IF 2.562
Wan-Qian Guo,Shan-Shan Yang,Ji-Wei Pang,Jie Ding,Xian-Jiao Zhou,Xiao-Chi Feng,He-Shan Zheng,Nan-Qi Ren
In order to increase hydrogen yield and shorten the lag time, low frequency ultrasonic (LFU) pretreatment was applied to stimulate the bio-activity of the seed bacteria, using waste activated sludge as the inoculum. A pretreated, sterilized sludge sample was seeded as the substrate for bio-hydrogen production. A Unisense microsensor multimeter with a hydrogen microelectrode was employed to examine the hydrogenase activities at different ultrasonic densities and exposure times. The optimum conditions were achieved at an ultrasonic density of 125 W L−1 and a reaction time of 10 s. A maximum hydrogen yield of 13.03 mL H2/g TS was obtained by LFU pretreatment under these optimal conditions, which was 18% higher than that obtained when seeding the raw sludge sample. To gain a better understanding of the end product distribution, three-dimensional excitation–emission matrix (3D-EEM) fluorescence spectroscopy was performed to characterize the dissolved organic matter (DOM) and extracellular polymeric substances (EPS) in the sludge samples during the anaerobic fermentation process. Hydrogen production from NADH/NAD+ conversion was also proved by EEM.
研究领域:
环境工程
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生物技术
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微生物学
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DOI
高影响因子
CATALYSIS LETTERS
·
2025
·Vol. 155
·期 11
·1-12
·
IF 2.4
Lu Wei,Zhou Liang
Photocatalytic regeneration of nicotinamide adenine dinucleotide (NADH) is indispensable for the sustainability of enzyme-driven industrial processes. In this study, we developed a Z-scheme heterogeneous photocatalyst consisting of CdS and g-C 3 N 4 , which markedly enhanced visible-light absorption and photogenerated charge separation efficiency, and applied it to an in situ visible-light-driven NADH regeneration system. The synthesized catalyst afforded an exceptional NADH regeneration yield of 97.88% within just 15 min. Additionally, enzyme activity assays utilizing alcohol dehydrogenase (ADH) demonstrated that 67.60% of NAD + was successfully converted into the enzymatically fraction, 1,4-NADH. Furthermore, formate dehydrogenase (FDH) immobilized on ZIF-8 was integrated into this NADH regeneration system, enabling highly sustainable biosynthesis of formic acid. During a 2 h continuous reaction, the integrated system successfully accumulated a total formic acid concentration of 0.88 mM. This novel Z-scheme photocatalytic system thus presented a highly effective solution for regenerating active NADH, providing valuable insights and promoting the development of photocatalysis-driven enzymatic systems for sustainable solar-to-chemical energy conversion. Graphical
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DOI
高影响因子
危险品化学品经营许可证（带存储）