ELECTROANALYSIS
·
2021
·Vol. 33
·期 11
·2278-2285
·
IF 3.223
Yuan Xiang,Hao Huang,Dan Wang,Juan Du,Dongping Wu,Wanming Xiong,Yanping Hong,Jinyin Chen,Xiaoning Liao
Herein, an organometallic Au(III) catalyst was easily prepared via self-assembly method using chloroauric acid (HAuCl 4 ) and cetyltrimethylammonium bromide (CTAB). The electrode fabricated by the catalyst not only shows extended anodic potential window compared to that of Au nanoparticle-based electrode, but also possess the high electrocatalytic activity in the selective oxidation of Ochratoxin A. Under optimized conditions, the modified electrode exhibits a wide linearity range from 1.0×10 −7 to 1.0×10 −5 mol/L and a low detection limit of 2.9×10 −8 mol/L (S/N=3). Furthermore, the electrochemical sensor possesses good recoveries between 93.5 % and 98.2 % in real sample analysis, indicating high accuracy in real sample analysis.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Tingting Zhao,Min Zhang,Qi Peng,Xucong Lin,Zenghong Xie
Herein, a facile protocol of simple DNA adsorption on UV-initiated polymerization supports was proposed for effectively fabricating aptamer-based affinity monolithic column. Hydrophilic cationic monolith with an excellent mechanical stability was achieved within 7 min and then massive aptamers were directly bound by DNA charge-dependent adsorption. Strong cationic quaternary ammonium-based monomer was employed to provide effective and stable positive charge surface for aptamer immobilization in a wide range of pH. An ultra-high aptamer coverage density of 6813 pmol/μL was achieved to gain a highly specific online recognition performance. Limitations such as low aptamer capacity, tedious modification and time-consuming reactions in the traditional biological or covalent modification strategies were avoided. By using ochratoxin A (OTA) as the given analyte, the selective recognition and high recoveries were successfully achieved, and little cross-reactivity towards OTB analogue was only 0.5% even if the content of OTB got up to 125 folds of OTA. Applied to sample analysis, the satisfactory discriminations of trace OTA were obtained at 93.9 ± 1.9% – 96.5 ± 1.7%（n = 3）in beer, wheat and chicken liver samples. It might light a cost-effective access to efficiently preparing high-performance affinity monoliths towards the selective in-tube microextraction of OTA.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Li Liu,Qingwen Huang,Zafar Iqbal Tanveer,Keqiu Jiang,Jinghui Zhang,Hongye Pan,Lianjun Luan,Xuesong Liu,Zheng Han,Yongjiang Wu
We report herein a “turn off-on” fluorescent sensor based on ZnCdSe quantum dots (ZnCdSe QDs) and self-assembled zinc porphyrin for sensitive and rapid detection of ochratoxin A (OTA). Using environmentally friendly dodecyl dimethyl betaine as “soft template”, zinc 5, 10, 15, 20-tetra(4-pyridyl)-21H-23H-porphine (ZnTPyP) was self-assembled into nanorods (SA-ZnTPyP) through a green process. The OTA detection process involves two steps: (1) the fluorescence of ZnCdSe QDs is significantly quenched by SA-ZnTPyP, and (2) the combination between SA-ZnTPyP and OTA releases ZnCdSe QDs from their quenchers such that the fluorescence is recovered. Under the optimized experimental conditions, the newly developed fluorescent sensor showed a linear response in concentration range 0.5–80 ng mL −1 , with a detection limit of 0.33 ng mL −1 . The excellent applicability of the system has been verified in milk and coffee samples, with relative recoveries between 98.33% and 103.70%. The proposed fluorescent sensor exhibits excellent selectivity and high sensitivity, and thus could be a promising tool for on-site determination of OTA in food safety control and environmental monitoring.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 3
·363-368
·
IF 1.248
Ma Lian,Xu Bin,Liu Leijing,Tian Wenjing
A label-free fluorescent aptasensor for specific and ultrasensitive monitoring ochratoxin A(OTA) was developed using the specific aptamer of OTA(OSA) as recognition element, an aggregation-induced emission(AIE) molecule(a 9,10-distyrylanthracene with two ammonium groups, DSAI) as a fluorescent probe, and graphene oxide(GO) as a quencher. In the absence of OTA, the AIE probe DSAI and OSA complex(DSAI/OSA) is adsorbed on the GO surface, and the fluorescence of DSAI will be quenched efficiently via the fluorescence resonance energy transfer(FRET) from DSAI to GO. Upon the OTA addition, a more stable complex(OSA-OTA) is formed and released from GO. Meanwhile, DSAI and OSA-OTA can form a new complex(DSAI/OSA-OTA), then the fluorescent signal of DSAI recovers gradually. Therefore, by introducing GO and DSAI, the fluorescence signal of DSAI can be easily turned from “off” to “on” after the addition of OTA, and the ultrasensitive detection of OTA by monitoring the change of the fluorescence signal of DSAI can be readily realized. The detection limit of the assay can reach 0.324 nmol/L with a linear detection range of 10—200 nmol/L. And the aptasensor exhibits high selectivity for OTA against other analogues. Moreover, it has been successfully applied for the detection of OTA in red wine samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Ke-Jing Huang,Hong-Lei Shuai,Ying-Xu Chen
Sensitive detection of ochratoxin A (OTA) plays an important role in the field of food safety control. In this study, we are reporting for the first time an electrochemical biosensor which employs the integration of MoSe 2 nanoflowers , Au nanoparticles (AuNPs), aptamer and the guanine-rich complementary DNA sequence for the sensitive detection of OTA. MoSe 2 nanoflowers are prepared by a simple hydrothermal method and used as a promising sensing platform based on its large surface area. The methylene blue is used as a tracer and a guanine-rich complementary DNA sequence is designed to bind with the unbound OTA aptamer for signal amplification . The aptamer is immobilized on the AuNPs/MoSe 2 modified electrode surface through Au–S interaction. Since OTA can competitively bind with the aptamer due to their high affinity, the presence of OTA will induce the decrease of guanine-rich DNA on the electrode surface , in turn reduces the signal of methylene blue which can specifically adsorb to the guanine units of DNA. The proposed assay shows a good linear relationship within the range of 0.0001–1 nM with a detection limit of 0.08 pM for OTA. The developed biosensor has been applied to determine OTA concentration in wine samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Yingshuai Liu,Jie Yu,Ying Wang,Zhenya Liu,Zhisong Lu
A novel and ultrasensitive aptasensor based on shielding effect-induced inhibition of fluorescence resonance energy transfer (FRET) was developed for detection of Ochratoxin A (OTA). The aptasensor is composed of a free OTA aptamer, a gold nanoparticle coated with biotinylated partially complementary DNA (biotin-cDNA-AuNP), and a Cy3-conjugated streptavidin, each of which acts as a recognition molecule, energy acceptor, and energy donor, respectively. In the absence of target, a half of OTA aptamer hybridizes with biotin-cDNA-AuNP, while another half of the aptamer plays a shielding cover to block the specific interaction between biotin and streptavidin, resulting in inhibition of FRET from Cy3-conjugated streptavidin to AuNP. In the presence of OTA, however, strong and specific interaction between aptamer and OTA reduces the shielding effect-based FRET inhibition, thus leading to dose-dependent fluorescence decrease. Under optimal condition, a limit of detection (LOD) of 1.4 pg mL −1 and a wide assay range from 2.5 pg mL −1 to 1 μg mL −1 was achieved for OTA detection in PBS. A negligible interference from other mycotoxins demonstrates a good selectivity of the developed aptasensor. The proposed method was also successfully applied to measure OTA in spiked wheat and green coffee bean extractives, demonstrating a great feasibility for analysis of various real samples. In addition, the unique shielding effect-based aptasensor could be adapted to the detection of other small molecules with poor immunogenicity by simple change of the aptamer.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiayu Chen,Liyuan Zhang,Runzhong Yu
A nucleic acid aptamer based thermally oxidized porous silicon/zinc oxide microarray chip was constructed for the detection of ochratoxin A. The hybrid chains formed by aptamer and complementary chains labeled with fluorescent groups and fluorescent burst groups were used as recognition molecules, and the detection of toxins was accomplished on the chip by the principle of fluorescence signal burst and recovery. The modified QuEChERS method was used for sample pretreatment and the performance of the method was evaluated. The results showed that the linear range was 0.02 ∼ 200 ng/kg with the detection limit of 0.0196 ng/kg under the optimal detection conditions. The method was applied to different cereals with the recoveries of 90.30 ∼ 111.69 %. The developed microarray chip has the advantages of being cost-effective, easy to prepare, sensitive and specific, and can provide a new method for the detection of other toxins.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinqi Long,Qi Wu,Lu Yang,Longyingzi Xie,Lanrui Ma,Qiyang Zhao,Yongliang Cui,Yue He,Yaohai Zhang
Aptasensors for detection of ochratoxin A (OTA) have been extensively studied, but the majority of them require costly and large-scale equipment as signal readers. Herein, a photothermal aptasensor capable of portable detection of OTA through a thermometer was developed on basis of aptamer structural switching and rolling circle amplification (RCA)-enriched DNAzyme. Oligonucleotides and alkaline phosphatase (ALP) modified magnetic beads were prepared. The binding of aptamers to OTA led to the release of ALP labeled complementary DNA. After magnetic separation, ALP catalyzed the padlock dephosphorylation, inhibiting the subsequent RCA reaction. This process converted the OTA concentration into the amount of the photothermal reagent oxTMB produced from the catalytic reaction induced by RCA-enriched DNAzyme. Under the optimal conditions, the detection limit (LOD) of this aptasensor was 2.28 nM in a clean buffer, while the LOD reached 2.43 nM in 2 % grape juice. The good performance of the photothermal aptasensor makes it possible to measure OTA pollution in low resource environments.
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Food Bioscience
·
2024
·
IF 4.8
Ailing Lei,Mingda Li,Siqi Zhuo,Qianjin Li,Jianlin Li
Ochratoxin A (OTA) with high toxicity is widely present in agricultural products, posing an enormous threat to human and animal health. Thus, simple, rapid, sensitive and selective detection of OTA is highly necessary. Herein, we have developed a new label-free fluorescence and naked-eye detection platform for ochratoxin A based on the interaction between OTA-antiparallel G-quadruplex(G4)/crystal violet (CV). CV and OTA aptamer could form a G4 structure with high fluorescence intensity. When OTA was present in the G4/CV mixture, it replaced CV and bound to the OTA aptamer, and then the fluorescence of the mixture remarkedly quenched. The interaction among OTA and G4/CV had been revealed by fluorescence, ultraviolet and circular dichroism (CD) spectra. The detection process could be performed by naked-eye and could be completed within 10 min. The established method showed 0.1–1000 ng/mL linear detection range with 0.07 ng/mL limit of detection (LOD). The recovery rates in cereal samples were in the range of 76–105%. Compared with the classical ELISA, the recovery rates and the detected concentration of OTA in the naturally contaminated cereal samples were consistent. The new method can provide a simple, rapid and cost-effective, label-free and naked-eye detection of OTA with high specificity, good repeatability and high precision.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yingli Guo,Zhixiong Zheng,Bingyu Fu,Shengxin Wen,Yu Zhou,Xingping Zhang,Hualin Yang
Ochratoxin A (OTA) as a mycotoxin has strong carcinogenicity and can affect human health. Thus, it is necessary to establish an accurate method to detect OTA. Herein, a ratiometric absorbance and fluorescence dual model immunoassay based on ZIF-8@uranine (ZIF-8@UR) and p -nitrophenol (PNP) was developed. In this new immunoassay, alkaline phosphatase-labeled secondary antibodies (IgG-ALP) were used to associate OTA with signal output. The IgG-ALP can hydrolyze 4-nitrophenyl phosphate salt (PNPP) to generate yellow PNP and phosphate (Pi). Then, the Pi triggered the decomposition of ZIF-8@UR to release UR with a fluorescence signal. The absorbance ratio of PNP and UR was taken as a ratiometric absorbance signal. The ratiometric absorbance and fluorescence modes exhibited linear ranges of 0.781–50.0 ng/mL and 1.563–100.0 ng/mL, with limits of detection (LODs) of 0.112 ng/mL and 0.147 ng/mL, respectively. Meanwhile, the new immunoassay showed high specificity for OTA and satisfactory recovery rate in actual samples.
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TALANTA
·
2019
·
IF 4.916
Yiqiong Chen,Xinyue Ding,Dandan Zhu,Xucong Lin,Zenghong Xie
Nonspecific adsorption is a challenge of specific recognition on aptamer-based affinity monoliths. Here, a novel highly hydrophilic polyhedral oligomeric silsesquioxane (POSS)-containing aptamer-based hybrid-silica affinity monolith with a good recognition nature was prepared and used for specific discrimination of ochratoxin A (OTA). A homogeneous polymerization mixture consisted of POSS chemicals, hydrophilic monomers and aptamer solution was directly polymerized via the “one-pot” method. Preparation and characterization of the resultant affinity monolith were studied in detail. A highly hydrophilic nature was obtained and the typical hydrophilic interaction liquid chromatography (HILIC) was observed when acetonitrile (ACN) content in mobile phase was 25%, which reached the highest hydrophilicity of POSS-based hybrid monoliths. By using OTA as model analyte, the nonspecific adsorption was effectively suppressed. The recovery of the analogue ochratoxin B (OTB) was only about 0.1% even if the content of OTB was 50 times more than OTA, which was much better than other POSS-containing monoliths and polar siloxane-based hybrid monoliths. Applied to beer samples, the adsorption of background materials was drastically resisted, and efficient recognition of OTA was obtained with the recoveries of 94.9–99.8%. Much less disturbance was observed than that occurred in hydrophobic POSS-based affinity monolith. It lights an attractive implement with high hydrophilicity and specificity for online selective recognition of OTA.
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TOXICON
·
2025
·
IF 2.4
Jingyi Yang,Hao Xu,Ruiqi Ye,Yu Zhang,Jiale Wu,Erhui Jin,Xiaojin Li,Mixia Cao,Shenghe Li,Chang Liu,Lei Li
Ochratoxin A (OTA) is a prevalent contaminant in feed and poses a serious threat to the poultry industry and public health. The liver is the primary target of OTA, and oxidative stress alongside consequent inflammation, is considered the main contributor to OTA-induced liver damage. Astragaloside IV (AS-IV), a key constituent of the traditional Chinese medicinal herb Astragalus membranaceus, exhibits diverse pharmacological properties, including anti-inflammatory, antioxidant, immunoregulatory, and organ-protective effects. However, whether AS-IV can ameliorate OTA-induced liver damage remains uncertain. In the current investigation, we investigated the effect of AS-IV on OTA-induced liver damage in chicks and elucidated the underlying mechanisms. The results revealed that AS-IV inhibited OTA-induced increases in alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) activities. Additionally, AS-IV reversed the OTA-induced decrease in glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities, as well as the increase in malondialdehyde (MDA) content (P < 0.05). The results demonstrated that AS-IV is capable of mitigating mitochondrial damage and reducing the elevation of reactive oxygen species (ROS) in the chicken liver cell line LMH induced by OTA. Moreover, AS-IV was discovered to reverse the OTA-induced decrease in nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), NADPH quinone oxidoreductase 1 (NQO1) expression, and the increase in Kelch-like ECH-associated protein 1(Keap1) expression. Meanwhile, AS-IV also counteracted OTA-induced NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome expression. Taken together, our findings suggested that AS-IV ameliorated OTA-induced hepatotoxicity in chicks by regulating the Nrf2 and NLRP3 signaling pathways.
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