SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Runzi Zhang,Xiaoyu Yang,Shunbi Xie,Bo Wu,Yao Liu,Yi He
Herein, target-induced catalytic hairpin assembly (CHA) integrate with a pH-responsive DNA triple helix structure is first used to construct a recyclable layered covalent organic framework ( L -COF) @Au as a SERS substrate for the ultrasensitive detection of ofloxacin (OFL). Firstly, a large specific surface area and abundant -NH 2 groups of the L -COF serves as an ideal carrier for Au NPs, enabling the generation of a strong and uniform electromagnetic field. Meanwhile, target-triggered catalytic hairpin assembly (CHA) is utilized to activate an efficient DNA walker system, thereby achieving significant amplification of the SERS signal. Notably, pH-responsive folding and unfolding of the DNA triplex can enables capture and release of the SERS tag (Au@4-NTP@Ag) to achieve regeneration of the SERS substrate, thus allowing multiple reuse cycles. Furthermore, a synergistic plasmonic enhancement effect was realized between the COF@Au substrate and the SERS tag, resulting in a broad dynamic detection range from 1.0 × 10 −13 mol/L to 1.0 × 10 −6 mol/L and a low limit of detection (LOD) of 5.79 × 10 −14 mol/L. The biosensor demonstrates accurate quantification in real food samples, confirming its practical applicability. In addition, the methodology simplifies experimental procedures and reduces operational costs. Overall, this study presents a highly selective and responsive sensing approach for constructing renewable SERS-based biosensors, providing a robust and prospective platform for the monitoring of antibiotics in food safety applications.
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Toxics
·
2026
·Vol. 14
·期 6
·457
·
IF 4.9
Xu Ding,Xin Li,Haojie Liu,Zhong Li,Yangchun Xia,Yanpeng Liang,Honghu Zeng,Xiaohong Song
Ofloxacin (OFL) and sulfamethoxazole (SMX) are common co-occurring antibiotic contaminants in aquatic environments, yet their long-term combined toxicity to freshwater fish remains poorly elucidated. In this study, adult mosquitofish (Gambusia affinis) were used as a model to investigate histopathological alterations, oxidative stress responses, gene expression, and gut microbiota changes after 30 days of exposure to environmentally relevant concentrations of OFL and SMX (0 ng/L, 50 ng/L, 1 μg/L, and 20 μg/L), either individually or in combination. The results showed that both single and combined exposures induced liver and intestinal damage. Oxidative stress responses exhibited clear tissue specificity, with activation of antioxidant defenses in the liver, whereas the intestine was mainly characterized by decreased SOD and GST activities, as well as reduced MDA content. Changes in gene expression were relatively limited, with significant alterations observed only in hepaticsod2andhsp90and intestinalhsp70in certain treatment groups. Gut microbiota analysis showed that OFL exerted a stronger disruptive effect than SMX, as reflected by increased alpha diversity, reduced abundance of core genera, and functional remodeling, whereas combined exposure triggered weaker microbial community restructuring relative to single exposures. Overall, OFL and SMX induced tissue-specific toxicity in mosquitofish by causing tissue injury, oxidative stress imbalance, and gut microbiota dysbiosis, with OFL showing the stronger overall effect.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jinhu Yun,Yan Liang,Juntao Guo,Lu Jiang,Hongxiang Zhu,Hainong Song,Shuangfei Wang
Ofloxacin (OFL) may cause potential ecological risks since it cannot be effectively removed by conventional biological degradation. The advanced oxidation process is an efficient approach for antibiotic removal. Herein, a heterogeneous system for hydrogen peroxide (H 2 O 2 ) activation by nanoscale zero-valent iron (nZVI) supported on powdered activated carbon (PAC) composite (nZVI@PAC) was proposed to proceed with the removal of OFL. Typically, OFL (25 mg/L) can be removed up to 94.7 % within 60 min ([H 2 O 2 ] = 10 mM, [nZVI@PAC] = 125 mg/L, pH = 6, T = 318 K). The effects of initial OFL concentration, pH, nZVI@PAC dosage, H 2 O 2 concentration, and different actual water matrices were examined to explore the application conditions and resistance of the nZVI@PAC/H 2 O 2 system. Meanwhile, the quenching experiment, PMSO probe experiment, and EPR results showed •OH and Fe IV O were the dominant reactive species in the system. Moreover, a sensible mechanism for OFL removal was proposed that nZVI@PAC aggregates OFL on its surface by adsorption, and then persistent free radicals (PFRs) and nZVI on the PAC degrade OFL by activating H 2 O 2 to produce •OH and Fe IV O. Furthermore, the effect of micro-electronic iron-carbon composite was proved by electrochemical experiments and density functional theory (DFT) calculations. Moreover, the degradation pathway of OFL in the nZVI@PAC/H 2 O 2 system and the toxicity assessment of intermediates were also given. Meanwhile, sequential runs and continuous flow column experiments showed nZVI@PAC/H 2 O 2 system had high stability. This work provides an innovative strategy of an advanced oxidation process based on heterogeneous reactions for organic contaminants removal using metal–carbon catalysts.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Qianqian Wang,Baoshan He,Yao Liu,Ligen Wu,Wenhong Zhao,Dongdong Xie,Wenjie Ren,Yiwei Xu
Antibiotic residues in food and the environment pose significant risks to public health and safety, necessitating the development of rapid, portable, and efficient detection methods. Ofloxacin (OFL), a widely used antibiotic, is of particular concern due to its potential for contamination in milk and surface water. Current detection methods often require expensive instrumentation and complex procedures, limiting their applicability for on-site testing. This work addresses the critical need for a cost-effective, portable approach to reliably detect OFL residues. We developed a novel personal glucose meter (PGM)-based aptasensor utilizing a porous spherical cerium-based metal-organic framework (Ce-MOF) as a loading platform for glucose oxidase (GOx) and oligonucleotide sequences (Ce-MOF-GOx-cDNA). The hybrid probe, formed by conjugating Ce-MOF-GOx-cDNA with aptamer-modified magnetic beads, enabled specific recognition of OFL through nucleobase pairing. The sensor exhibited a detection range of 50 pg/mL to 500 ng/mL with a detection limit of 40 pg/mL under optimal conditions. The process showed excellent selectivity, stability, and reproducibility. Real-sample testing in spiked milk and surface water demonstrated recovery rates of 99.5% – 108%, with relative standard deviations of less than 4.7%. This study presents a portable and efficient strategy for detecting OFL residues using a PGM-based aptasensor. The method combines simplicity, rapid detection, and high sensitivity, offering significant potential for on-site applications in food and environmental safety monitoring.
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ELECTROANALYSIS
·
2016
·Vol. 29
·期 2
·602-608
·
IF 2.471
Zhiming Jiang,Qin Liu,Yuran Tang,Mingxiao Zhang
Pt−Au nanoclusters decorated on the surface of reduced graphene oxide (rGO/Pt−Au) was facilely prepared by one-pot electrochemical reduction. The morphology and composition of rGO/Pt−Au composites had been characterized by scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectrometry (EDX), fourier transform-infrared spectroscopy (FT-IR) and electrochemical methods. Ofloxacin is a member of synthetic quinolones which has been widely used for the treatment of common diseases in humans and animals. The performance of the rGO/Pt−Au nanocomposite toward the oxidation of ofloxacin was compared with the other similar nanostructures like rGO/Pt and rGO/Au. In the optimized conditions, two linear calibration curves were obtained, from 0.08 to 10 μM and 10 to 100 μM ofloxacin. A detection limit of 0.05 μM ofloxacin was observed at pH 5.7 for the GCE/rGO/Pt−Au. The proposed sensor was successfully applied to determine ofloxacin in tablets and human urine samples and the results were satisfactory.
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Desalination and Water Treatment
·
2024
·
IF 1
Zicai Zhao,Yuanyuan Zhang,Shuangfei Wang,Hongxiang Zhu,Jianhua Xiong
ABSTRACT In this study, zinc oxide (ZnO) supported copper oxide (CuO) catalyst was prepared and characterized. Ofloxacin (OFL) was selected as the target pollutant to study the catalytic activity of CuO/ ZnO. The characterizations of CuO/ZnO were studied using X-ray diffraction, field emission scanning electron microscopy and X-ray photoelectron spectroscopy etc. The catalytic activity of CuO/ZnO on OFL degradation efficiency was systematically examined under different experimental conditions: including different catalyst doses, O 3 flow rates, initial pH values and OFL concentrations. The catalytic activity achieved maximal when Cu:Zn was 1:5. The results showed that the degradation of OFL increased with the increase of CuO/ZnO dosage, O 3 flow rate and pH value, but decreased with the increase of OFL initial concentration. Under selected initial conditions (80 mL/min ozone flow, 20 mg/L OFL solution, and 0.63 g/L catalyst dosage), the O 3 +CuO/ZnO process significantly improved OFL removal efficiency due to the production of hydroxyl radical ( • OH), which was approved by tert-butanol (TBA). It was confirmed that CuO and ZnO showed synergistic effect on the catalytic ozonation of OFL. The degradation efficiency of OFL was only slightly decreased after five repeated times, indicating that CuO/ZnO had well catalytic stability.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhengxu Liu,Yihui Hu,Yushi Huang,Zhengrui Zhang,Yuancai Lv,Minghua Liu
In this study, sludge-derived biochar loaded Co 3 O 4 composite (Co 3 O 4 @SDBC) were successfully synthesized through cobalt impregnation and secondary calcination of sludge-derived biochar precursors. The experimental results demonstrated that the Co 3 O 4 @SDBC catalyst performed exceptionally well for peroxymonosulfate (PMS) activation, with a high degradation efficiency (>99 %) of ofloxacin (OFL) within 10 min under the optimal conditions (a catalyst dose of 0.10 g•L −1 , a PMS dose of 0.975 mM and an initial pH = 6.4), and showed good adaptability across a broad pH spectrum, under the interference of the anions and humic acid (HA), as well as in various water matrices. The results of quenching experiments revealed that the non-radical pathway (especially 1 O 2 and electron transfer) was the dominated reactive ingredients in the PMS activation process induced by Co 3 O 4 @SDBC. Notably, the online electrochemical tests and density functional theory (DFT) results indicated the presence of biochar carrier not only effectively lowered the escape of Co ions, while enhanced the steadiness of Co 3 O 4 @SDBC, but also accelerated the electron transfer, promoted the adsorption and cleavage of PMS, facilitated the redox cycle between Co(II) and Co(III), reduced the reaction energy barriers simultaneously, thereby making it more favourable for the generation of 1 O 2 . Furthermore, the toxicity assessment suggested that the cumulative toxicity of OFL decreased throughout degradation. Overall, this work mechanistically revealed the enhancement of biochar on the Co 3 O 4 @SDBC catalyst, offering a viable method to activate PMS for antibiotic removal and achieving a mutually beneficial strategy for sludge waste resource utilization and environmental remediation.
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Russian Journal of Physical Chemistry A
·
2026
·Vol. 100
·期 10
·2407-2418
·
IF 0.7
Xuan Liu,Huang Jinliang,Guo Feifei,Chang Liangliang,Zhou Chunsheng,Xi Zengzhe
This study presents a novel strategy to significantly enhance the piezocatalytic performance of BiFeO 3 for antibiotic degradation by integrating cation co-doping and noble metal decoration. To address the inherent limitations of rapid charge recombination and insufficient active sites in pristine BiFeO 3 , Gd, and Zr co-doped BiFeO 3 (BGFZ9) NPs were first synthesized via a sol-gel method. Subsequently, Ag NPs were uniformly deposited on the BGFZ9 surface through a facile photochemical reduction process to construct Ag/BGFZ9 nanocomposites. Comprehensive characterizations including XRD, XPS, TEM, and VSM confirmed the successful formation of the perovskite structure, uniform dispersion of Ag NPs, and tailored magnetic properties. The optimized Ag2/BGFZ9 catalyst demonstrated remarkable piezocatalytic and piezo-photocatalytic activity towards ofloxacin, achieving degradation efficiencies of 82.54 and 86.98% within 120 min under ultrasonic and combined ultrasonic/visible-light irradiation, respectively. The corresponding first-order rate constants were 0.0147 and 0.0164 min –1 , representing a significant enhancement over the undoped and unmodified counterparts. The superior performance is attributed to the synergistic interplay of multiple factors: (1) the Gd/Zr co-doping induced lattice distortion and optimized band structure, enhancing the intrinsic piezoelectric response; (2) the formation of a Schottky junction at the Ag/BGFZ9 interface, which acted as an efficient electron sink to suppress charge carrier recombination; and (3) the LSPR effect of Ag NPs under visible light, which broadened light absorption and generated hot electrons. Radical trapping experiments and EPR spectroscopy identified \( \bullet {\kern 1pt} {\text{O}}_{2}^{ - }\) and \( \bullet {\kern 1pt} {\text{OH}}\) as the primary reactive oxygen species. The catalyst maintained 82.13% of its initial activity after five consecutive cycles, demonstrating excellent stability and magnetic recoverability. This work elucidates a synergistic piezoelectric-plasmonic coupling mechanism and provides a viable design principle for developing efficient, stable, and magnetically separable multiferroic catalysts for advanced oxidation processes in wastewater remediation.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Huatao Sun,Huiyan Chen,Sha Chen,Zhoujie Pi,Xin Wu,Chun Yang,Haiyan Ai,Ding Li
Microbial synthesis enabled the incorporation of Fe into α-Mn 2 O 3 . • Biogenic Fe-doped α-Mn 2 O 3 surpasses its chemogenic counterpart in PMS activation. • The degradation mechanism of ofloxacin was elucidated. • Degradation intermediates of ofloxacin were evaluated for biotoxicity. For the same material, adopting a microbially guided fabrication method rather than conventional chemical synthesis can intriguingly enhance its catalytic performance. In this study, cost-effective Fe-doped α-Mn 2 O 3 was microbially synthesized to achieve higher degradation efficiency of ofloxacin (OFL) in a peroxymonosulfate (PMS)-based advanced oxidation process. Structural characterization reveals that microbially mediated Fe doping into α-Mn 2 O 3 induced lattice contraction, increased the Mn(III) fraction, and retained the MnO 6 octahedral framework. Biogenic Fe-doped α-Mn 2 O 3 achieved nearly complete degradation (99.98%) of OFL within 20 min (0.46 min −1 ), with the reaction rate constant elevated 8.51-fold compared to its chemically synthesized counterpart. Meanwhile, electrochemical analyses demonstrate that it exhibited higher anodic current density, faster electron transfer kinetics, and smaller charge transfer resistance–features more favorable for PMS activation to generate reactive oxygen species, thereby enabling efficient OFL degradation, with the oxidative contribution following the order of 1 O 2 > SO 4 •− > • OH. Density functional theory calculations reveal that Fe incorporation into α-Mn 2 O 3 strengthened molecular adsorption and reduced the surface electron escape barrier. This work provides a biologically inspired paradigm to boost the catalytic performance of abundant, cost-effective transition metal oxides without reliance on complex fabrication approaches. Download: Download high-res image (206KB) Download: Download full-size image
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JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Yuxuan Yang,Zhaoping Zhong,Jiefei Li,Haoran Du,Zhaoying Li
Quinolone antibiotics (QNs) can be potential hazard to environment and human. Combination of experimental and theoretical studies was used to analyze the adsorption properties of norfloxacin, ciprofloxacin and ofloxacin on modified thermally activated kaolin (KL). Main factors (calcination temperature, dose, pH, cations and regeneration) affecting the adsorption were discussed. Adsorption processes fit the pseudo-second order kinetic and Langmuir model well. The adsorption removal of norfloxacin, ciprofloxacin and ofloxacin can reach 88.53%, 89.43% and 91.46%, respectively. Cations inhibited adsorption, and AlS-KLB can maintain 80% efficiency in five cycles under optimal conditions. Simulations showed that the materials had good adsorption capacity for QNs, and the "①" of KL had the best capacity. Simulations explain the adsorption mechanism: F, H, O atoms of QNs are covalently bonded to O atoms from KL, Al 2 O 3 and Al (OH) 3 , C atoms from amorphous carbon and H atoms from C-H and Al (OH) 3 . The Al atoms of Al 2 O 3 and Al, Si atoms of KL are ionically bonded to F, H, O atoms of QNs. This study shed new light on the removal of QNs by providing low-cost and efficient modified KL and elucidating the adsorption mechanism in conjunction with DFT simulations.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Chu Dai,Xike Tian,Yulun Nie,Wei Fu,Jiatang Wang
Most researches focus on design and optimization of heterogeneous Fenton catalyst itself to drive H 2 O 2 decomposition into • OH radicals. In this study, we reported the significant impact of the interaction mode of H 2 O 2 and CuMnO 2 (namely H 2 O 2 occurrence form) on the Fenton-like performance. The interaction mode of H 2 O 2 largely exposed to Cu atom active site (CMH-L) led to a superior ofloxacin (OFX) degradation efficiency of 93% compared with H 2 O 2 middle exposed around the Cu atoms (CMH-M, 84%) and small part exposed around the Cu atoms (CMH-S, 50%). The effect of H 2 O 2 occurrence forms on catalytic activity were confirmed by analyzing their fraction and the relevant rate constant with the correlation was 98%. And the relevant interfacial reaction mechanism was detailed explored based on density functional theory calculation, a series of probe experiments and quantitative analysis of H 2 O 2 utilization efficiency and • OH generation. It revealed that Cu atom arrangement on CuMnO 2 can induce the different H 2 O 2 occurrence form, and compared with CMH-M especially CMH-S (1.486 and 1.591 Å), CMH-L has the shortest elongation of O-O bond (1.477 vs. 1.468 Å in free H 2 O 2 ) together with the smallest O-H 1 and O-H 2 (0.988, 1.022 Å), which were more likely to decomposition H 2 O 2 into • OH, while it was more likely to inefficient transformation H 2 O 2 into O 2 in CMH-S system. Thus, this work provided a new insight for improving the heterogeneous Fenton catalytic performance via altering the interaction mode of H 2 O 2 and catalyst, which should be the fundamental reason for efficient H 2 O 2 activation into O 2 or • OH.
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TALANTA
·
2021
·
IF 6.057
Yukun Huang,Chong Wang,Qiming Wei,Yaning Song,Pengfei Chen,Lijun Wang,Xiao Yang,Xianggui Chen
As the light-harvesting “antenna”, G-rich oligonucleotides (such as the G-quadruplex) can interact with lanthanide (III) to bring a luminescent enhancement response. In this study, phenomenon of luminescent enhancement of G-triplex/terbium (III) (G3/Tb 3+ ) and interaction between G3 and Tb 3+ were first reported and characterized. Based on G3/Tb 3+ luminescence, a label-free aptasensor for the detection of ofloxacin (OFL) residues in the food was developed. The OFL triggered the action of rolling circle amplification (RCA) allowed for the amplification product of G3-forming sequences in the single-stranded DNA, which promoted the conformational transition of the G3/Tb 3+ complexes once the addition of Tb 3+ . Under the optimal conditions, the logarithmic correlation between the G3/Tb 3+ luminescence intensity and the concentration of OFL was found to be linear in the range of 5–1000 pmol L −1 (R 2 = 0.9949). The limit of detection was 0.18 pmol L −1 (3σ/slope). Additionally, the good recoveries of 90.19–108.89 % and the relative standard deviations values of 0.59–5.87 % were obtained in the application of the aptasensor detecting OFL in the practical samples. These results confirmed that the present aptasensor has a good analytical performance and bright prospect for detecting ofloxacin residues in food.
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