查找使用阿拉丁产品的科研文献

Inhibitory effect of Vibrio parahaemolyticus phage-functionalized PCL films on the host bacterium

Han Deng,Tian Shi,Liang Ke,Wei Xu,Mengni Zhou,Huajuan Wang,Min Zhou

An environmentally stable Vibrio parahaemolyticus phage 475s1 was isolated. • Phage 475s1 was stably immobilized onto biodegradable PCL films. • The films displayed strong antibacterial efficacy in vitro and in salmon. Vibrio parahaemolyticus ( V. parahaemolyticus ) is recognized as a major foodborne pathogen with global prevalence. The overuse of traditional antibiotics has not only induced the emergence of drug-resistant strains, but also posed a potential threat to human health through the food chain. Therefore, the development of green and precisely targeted antibacterial agents is urgent. In this study, a V. parahaemolyticus phage 475s1 was isolated and exhibited remarkable stability over a broad temperature range (0-70 °C), pH range (4–11) and ultraviolet (UV) irradiation durations (0-60 min). It was capable of lysing 12 out of 23 tested V. parahaemolyticus strains, with a burst size of 110 plaque-forming units (PFU) per infected cell. The phage 475s1 was immobilized onto the surface of biodegradable polycaprolactone (PCL) films. The results showed that the binding density of phage on the PCL films reached 0.04 phages/μm 2 , while the host-capturing density was approximately 0.05 cells/μm 2 . After ultrasonic treatment for 20 min, 35% of the phages remained stably adsorbed on the PCL films. The phage-functionalized PCL films were further applied to artificially contaminated salmon samples. At an incubation temperature of 37 °C, the phage-functionalized PCL films exhibited significant antibacterial efficacy. Compared with the control group, the bacterial concentration decreased by 1.72 log CFU/mL at 2 h and 1.60 log CFU/mL at 3 h, respectively. Therefore, these findings indicate that the V. parahaemolyticus phage-functionalized PCL films hold great promise as a novel and effective antibacterial packaging material. This work provides a feasible green antibacterial strategy against seafood-borne V. parahaemolyticus , offering valuable theoretical support for the development of biodegradable active packaging for industrial aquatic preservation.

Construction and Properties of SPI/PLA-PCL Composite Coating on Pure Titanium Surface

Chunmei Wang,Congyi Zhu,Qi Zhang,Shuangsheng Zhang,Ling Zhang,Jiang Wu,Guoliang Zhang

Titanium and titanium alloys have been widely used in clinical implants such as dental implants due to their high strength and corrosion resistance. However, their inherent biological inertness and mismatch with the elastic modulus of human bone tissue restrict bone healing and reconstruction. In this study, pure titanium was first modified by micro-arc oxidation (MAO), and then a biodegradable soybean protein isolate (SPI)/polylactic acid (PLA)–polycaprolactone (PCL) composite coating was prepared by using the spin-coating method. The coating was systematically characterized by scanning electron microscopy (SEM), an energy spectrometer (EDS), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and atomic force microscopy (AFM), confirming that the composite coating was successfully prepared. An evaluation of the physical and chemical properties showed that the introduction of SPI significantly improved the hydrophilicity, surface roughness and adhesion to the substrate of the coating, regulated the degradation rate, and reduced the elastic modulus to the range of 10–30 GPa, which matches human bone tissue. At the same time, it enhanced corrosion resistance. In vitro MC3T3-E1 osteoblast experiments showed that the coating containing 50% SPI showed better cell compatibility, adhesion ability and osteogenic inducibility, and all groups had good blood compatibility. The SPI/PLA–PCL composite coating effectively improves the biological activity of the pure titanium surface and provides a feasible strategy for the surface modification of titanium implants and bone defect repair.

Enhancing wound healing through sonodynamic silver/barium titanate heterostructures-loading gelatin/PCL nanodressings

Yusen Zhang,Shuai Ke,Xiao Hu,Shuangying Wang,Wanqi Peng,Xinhang Qian,Linghui Tian,Huijun Wu,Binghui Li,Xiantao Zeng,Lingling Zhang

Bacterial infections present a formidable challenge in surgical procedures, and are a major threat to wound healing. Sonodynamic therapy (SDT) is a non-invasive approach for fighting pathogens; however, it is hindered by the efficiency of sonosensitizers and effective antibacterial time. In this study, we developed a biocompatible nanodressing to improve the antibacterial efficacy and accelerate wound healing via SDT. Silver nanoparticles (NPs) were synthesized on tetragonal barium titanate (BTO) NPs to create an Ag@BTO heterostructure of sonosensitizers to improve their piezocatalytic activities, which were then incorporated into gelatin/polycaprolactone (PCL) to form Ag@BTO-gelatin/PCL nanofiber (ABT-gP NFs) dressings. The loading of Ag@BTO NPs resulted in ABT-gP NFs with better mechanical properties and excellent piezocatalysis, which produced reactive oxygen species and Ag + to kill bacteria during ultrasound (US) irradiation. Additionally, nanodressing released moderate amounts of silver ions without US, prolonging the antibacterial time, while promoting fibroblast migration. This approach was effective in killing Gram-positive and Gram-negative bacteria (100 % and 90.8 %, respectively), promoting cell migration in vitro and accelerating wound healing without adverse effects in vivo . This study extends the potential applications of ultrasound-triggered nanodressing to the field of wound healing.

Humidity-responsive PCL/ICs/PVA pad with directional water transport for fruit preservation

Xuling Song,Zhengfeng Wang,Minxin Zhou,Yuwei Li,Yujie Wei,Yi Luo,Jianfeng Li,Bei Xu,Baijun Shang,Qingshan Duan

A smart humidity-responsive antimicrobial pad with directional water transport (DWT) was developed to address the rapid spoilage of fruits after harvest due to high humidity and water accumulation. This pad was fabricated from polycaprolactone/cinnamaldehyde β-cyclodextrin inclusion complexes/polyvinyl alcohol (PCL/ICs/PVA) using electrospinning and co-precipitation methods. The inclusion complexes formed by β-cyclodextrin and the guest molecule cinnamaldehyde (CIN) undergo crystalline and conformational changes upon moisture absorption, generating gaps or channels enabling humidity-responsive release of CIN. Meanwhile, capillary action, water uptake by the hydrophilic layer, and reverse water penetration resistance from the hydrophobic layer jointly promote DWT. In the PCL/ICs/PVA pad, the hydrophobic PCL coalesces free water molecules in the air to form tiny droplets to promote DWT, ICs release CIN in response to humidity, and the hydrophilic PVA absorbs droplets, together enhancing the antibacterial performance of the pad under high humidity conditions. The 14 PCL layer (electrospun from 14 wt% PCL solution) with honeycomb-like morphology was beneficial for loading ICs and humidity response. At 98 % RH, the pad with 33.3 % ICs sustained CIN release for 168 h and extended strawberry shelf life. This study holds significant implications for fruit preservation in high-humidity environments.

Functionalized 3D-Printed PCL/β-TCP Scaffolds with ZnO for Enhanced Bone Healing

Ayijiamila Mohetaer,Shan Rong,Zhang Jing,Wang Yuxin,Wan Xiaofei,He Qian,Ma Chunquan,Liu Bin,Song Fuxiang

Alveolar ridge deficiency remains a significant clinical challenge in dental implantology, often necessitating bone augmentation procedures. While autogenous bone grafting is considered the gold standard, its limitations, including donor site complications and limited availability, have prompted the development of alternative bone graft materials. In this study, we fabricated a novel polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP)@zinc oxide (ZnO) composite scaffold via direct ink writing (DIW) three-dimensional (3D) printing. The scaffold integrates a biomimetic porous architecture with a controlled ion-release strategy to enhance osteogenic performance. The characterization of the material confirmed excellent chemical stability, hydrophilicity, and tunable degradation behavior. The incorporation of ZnO facilitated the sustained release of bioactive ions (Ca 2+ , PO 4 3− , and Zn 2+ ), significantly improving the scaffold's bioactivity. In vitro assessments revealed that the scaffold with 2% ZnO (PTZ-2) exhibited optimal cytocompatibility and osteogenic differentiation capacity, promoting alkaline phosphatase activity and mineralized nodule formation in MC3T3-E1 cells. In a rat cranial defect model, the PTZ-2 scaffold achieved approximately 95% new bone area after 12 weeks. Histological analysis further confirmed the presence of continuous, highly mineralized bone matrix with excellent osseointegration capability. These findings collectively indicate that the 3D-printed PCL/β-TCP@ZnO composite scaffold has great potential for clinical translation in bone defect regeneration.

Oral delivery of WR-1065 by ROS-responsive PEG-PCL nanoparticles for radioprotection

Xiaona Lin,Xinxin Wang,Hongqi Tian

When a burst of reactive oxygen species (ROS) is induced after radiation exposure, a sufficient radioprotector drug must be quickly released to neutralize the ROS . An oral polymeric carrier of poly(ε-caprolactone)-b-poly(ethylene glycol)-b-poly(ε-caprolactone) was synthesized via the ROS-response linker thioketal (TK-linked PCEC) and used for delivery of the drug WR-1065. The drug-loaded nanoparticles (PCEC/WR-1065 NPs) had high WR-1065 content (19.7 % w/w) in the core and a particle size of 183 nm. The PCEC/WR-1065 NPs could protect the drug from degradation at gastric pH. More than 70 % of the TK-linked PCEC copolymer could be cleaved in 1 h. Therefore, a sufficient amount of WR-1065 could be quickly released from the PCEC/WR-1065 NPs to neutralize the burst of free radicals generated under irradiation. The in vitro cell uptake results of TK-linked PCEC NPs indicated that the NPs could be efficiently endocytosized by Caco-2 cells. Oral administration of the PCEC/WR-1065 led to survival benefit as well as protective effects on hematopoiesis and main organs with respect to the irradiation and oral free WR-1065 groups after 7.2 and 8.0 Gy total body irradiation, respectively. In vivo biodistribution studies indicated that the PCEC/WR-1065 NPs accumulated mainly in intestinal tissue. These results suggest that the design presented here provides a promising oral ROS-sensitive platform for acute radiation syndrome.

An investigation on sunlight-induced shape memory behaviors of PCL/TiN composites film

Guangming Tian,Guangming Zhu,Shuogui Xu,Tianning Ren

Plasmonic materials possess good optical properties in effectively converting solar energy into heat, here a series of sunlight responsive shape memory composites films based on acrylate-terminated Poly (ε-caprolactone) (PCL) and titanium nitride nanoparticles (TiN NPs) are prepared via base-catalyzed Michael addition. Compared with conventional free radical polymerization or physical blending, this paper constructs a network architecture from view of structural controllability, favoring a subsequent flexible and elastic materials as well as fine dispersion of TiN NPs in the cross-linked networks, the resultant materials show an outstanding mechanical and the incorporated TiN NPs enable rapid temperature increase over the melting temperature of PCL and trigger the shape change of the composite with good shape recovery rate. The effect of light density and TiN NPs concentration on composite film are elucidated and investigated systematically, it is found that the amount of TiN NPs content virtually has a negligible effect on the surface temperature of the films when subjected to a specific illumination, however, light intensity has a remarkable influence on it. Furthermore, an electronics device switch actuated by 160 mw cm−2 simulated sunlight irradiation is demonstrated and envisioned for flexible electronic applications.

Extrusion deposition 3D printed PCL/gel/n-HA composite scaffold for bone regeneration

Chenxin Wang,Jie Liu,Mingyue Lin,Rui Zhang,Yufan Li,Yubao Li,Qin Zou

Zinc oxide/silver bimetallic nanoencapsulated in PVP/PCL nanofibres for improved antibacterial activity

Min Hu,Chenwen Li,Xin Li,Min Zhou,Jianbin Sun,Fangfang Sheng,Sanjun Shi,Laichun Lu

The anti-infection ability and skin regeneration are important aspects on the progress of wound healing, which needs an ideal wound dressing that not only resists bacteria but also promotes skin regeneration. In this study, zinc oxide/silver/polyvinylpyrrolidone/polycaprolactone (ZnO/Ag/PVP/PCL) nanofibres were prepared through electrospinning. Firstly, zinc oxide nanoparticles (ZnONPs) and silver nanoparticle (AgNPs) were synthesized respectively. Secondly, the two nanoparticles were mixed with polyvinylpyrrolidone (PVP) and polycaprolactone (PCL) to obtain the nanofibres. The results of scanning electron microscopy (SEM) showed that ZnONPs and AgNPs were 40.07 ± 9.70 nm and 37.46 ± 12.02 nm, respectively. After electrospinning, the nanofibres were 368.22 ± 123.96 nm in diameter. Infrared spectroscopy revealed that ZnONPs/AgNPs bimetallic nanomaterials were physically embedded in the nanofibres. The antibacterial effects against Staphylococcus aureus and Escherichia coli of ZnO/Ag/PVP/PCL nanofibres were significantly better than these of the single metal material-loaded nanofibres. More importantly, the combination of ZnO and Ag reduced the cytotoxicity of ZnO/Ag/PVP/PCL bimetallic nanofibres toward fibroblasts. These findings demonstrated that ZnO/Ag/PVP/PCL bimetallic nanofibres should be of greater interest than the single metal nanomaterial-loaded nanofibres in inhibiting growth of bacteria.

Tuning the crystallinity and degradability of PCL by organocatalytic copolymerization with δ-hexalactone

Qilei Song,Yening Xia,Shuangyan Hu,Junpeng Zhao,Guangzhao Zhang

The copolymerization of ε -caprolactone (CL) with its structural isomer , δ -hexalactone (HL), or trimethylene carbonate (TMC) catalysed by diphenyl phosphate was investigated. Copolymers with molar fraction of CL units ranging from 54% to 95% were obtained. Molecular characterization exhibited random distribution of the two monomeric units (CL/HL or CL/TMC), owing to the extensive occurrence of transesterification scrambling reactions at high temperature. Compared with poly( ε -caprolactone) (PCL), the copolymers showed reduced crystallinity and enhanced enzymatic degradability as revealed by the analysis with differential scanning calorimetry and quartz crystal microbalance with dissipation, respectively. Interestingly, the incorporation of HL units showed a more profound impact than TMC. It is therefore considered that organocatalytic copolymerization with HL, a bio-renewable lactone, can serve as an effective method to regulate the properties of PCL.

Microscale electrohydrodynamic printing of biomimetic PCL/nHA composite scaffolds for bone tissue engineering

Xiaoli Qu,Peng Xia,Jiankang He,Dichen Li

Electrohydrodynamic printing provides an innovative strategy to fabricate high-resolution tissue-engineering scaffolds with fiber orientation and scale similar to native extracellular matrix. However, few studies have been conducted to incorporate functional nanobiomaterials into microscale fibrous structures. In this study, we presented to fabricate microscale poly (ε-caprolactone) (PCL) and hydroxyapatite nanoparticles (nHA) composite scaffolds with the average fiber diameter of 8.85±1.12   µm based on electrohydrodynamic 3D printing to better mimic collagen fibers and HA nanocrystals in natural bones. The composite scaffolds exhibited good biocompatibility and facilitated cell alignment and proliferation in vitro . This strategy might be useful to regulate cellular microenvironment in multiscale and multimaterial levels for improved tissue regeneration.

Attapulgite-doped electrospun PCL scaffolds for enhanced bone regeneration in rat cranium defects

Ting Dai,Jiayi Ma,Su Ni,Chun Liu,Yan Wang,Siyu Wu,Jun Liu,Yiping Weng,Dong Zhou,Ana Jimenez-Franco,Hongbin Zhao,Xiubo Zhao

Electrospun PCL scaffolds have been widely used for tissue engineering as they have shown great potential to mimic the structure of the natural extracellular matrix (ECM). However, the small pore size and low bioactivity of the scaffolds limit cell migration and tissue formation. In this study, PCL (polycaprolactone), PCL/PEG (polyethylene glycol), and PCL/PEG/ATP (nano-attapulgite) scaffolds were fabricated via electrospinning. To increase the porosity of the scaffolds, they were washed to remove water-soluble PEG fibers. Then the porous structure was measured using scanning electron microscopy (SEM) and atomic force microscopy (AFM), which showed an increased porosity when PEG fibers were removed in PCL/PEG and PCL/PEG/ATP scaffolds. Moreover, the mechanical properties were also analyzed in dry and wet conditions. In vitro mouse multipotent mesenchymal precursor cells were used to assess the biocompatibility of the scaffolds, and osteogenesis was analyzed using CCK-8 and real-time PCR (RT-PCR) methods. Moreover, in vivo μCT, histological and immunohistochemical analyses were conducted to evaluate new bone formation in rat cranium defect models. Washed PCL/PEG/ATP scaffolds were implanted into the cranium defects in rats for 4 or 8 weeks, better cell infiltration was observed in these scaffolds than in unwashed ones. The result demonstrated that washed PCL/PEG/ATP scaffold facilitated the differentiation of MSCs into osteoblasts compared with PCL scaffold, as proved by the increased expression of osteogenic key genes as well as Smad1, Smad4, and Smad5. Furthermore, in vivo studies demonstrated that using the ATP-doped electrospun PCL scaffold can improve the bone regeneration of rat cranium defects. Particularly, the PCL/ATP-30% scaffold has the best effect compared to the other scaffolds. The enhanced osteogenesis and bone repair were related to the PCL/ATP activated BMP/Smad signaling pathway.