INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Tian-hua Li,De-jin Ma,Su-yu Yang,Shu-tong Li,Jun-jie Yu,Yang Liu,Yang Yu,Liang Kong,Xue-tao Li,Juan Zang
Bacterial pneumonia remains a critical global health challenge due to antibiotic resistance, biofilm-mediated drug tolerance, and unregulated inflammatory damage. Conventional therapies fail to address these interconnected issues, necessitating innovative delivery systems that combine antibacterial and anti-inflammatory actions. Given the microacidic environment of infected tissues, we developed an environmentally responsive micelle (FA-Cip/Ag-Ms) capable of targeting pseudomonas aeruginosa infected lungs, reducing bacterial load and suppressing inflammation. The micelle is self-assembled from the biblock copolymer PEG-Hyd-PCL, which was acid-sensitive due to the presence of hydrazone bond. The antibacterial agent ciprofloxacin (Cip) and the anti-inflammatory andrographolide(Ag) were encapsulated, while folate acid (FA) served as a targeting ligand on the surface. We characterized FA-Cip/Ag-Ms by (Alhajj et al., 2022 [ 1 ] ) HNM, FT-IR, and TEM, indicating that the micelle was successful preparation. The drug release of Cip was 50.6 % at pH 7.4, whereas 77.6 % accumulative release was achieved at pH 5.4. The targeting ability of FA was demonstrated by fluorescence microscopy detection of bacteria and cells and in vivo tissue imaging detection. The antibacterial and biofilm-clearing abilities of FA-Cip/Ag-Ms were confirmed by Live/Dead staining, and quantitative biofilm staining. Flow cytometry and cytokine analysis revealed reduced pro-inflammatory M1 macrophages and increased anti-inflammatory M2 polarization, accompanied by decreased IL-6 and elevated IL-10 levels. In a murine pneumonia model, FA-Cip/Ag-Ms significantly reduced lung tissue damage, inflammatory cell infiltration, and bacterial colony formation, while exhibiting good biocompatibility. This functional system integrates active targeting and microenvironment responsiveness, offering a promising strategy to improve therapeutic effect for bacterial pneumonia.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yunbo Wu,Ye Ying,Yuhong Liu,Haijiang Zhang,Jianying Huang
Poly (vinyl alcohol) (PVA) and chitosan (CH)-based blend films were prepared via a simple mixing and casting method. The films were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and ultraviolet–visible spectroscopy (UV–vis). Mechanical properties, thermogravimetric (TG), derivative thermogravimetric (DTG), and the inhibition of biofilm formation against Pseudomonas aeruginosa PAO1 ( P. aeruginosa PAO1) of CH/PVA films were investigated. The results indicated that CH and PVA in the blend films interacted by physical crosslinks consisting of intermolecular hydrogen bonds and intermolecular hydrogen bonds with compatibility, and were more thermally-stable than that of PVA. CH addition decreased the tensile strength and the light transmittance. CH60:PVA40 film showed notable activity against adhesion and inhibited biofilm formation of P. aeruginosa PAO1, indicating that CH60:PVA40 film could be applied as an antimicrobial and biofilm inhibiting food packaging material .
研究领域:
生物材料学
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微生物学
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食品科学
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高分子科学
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抗菌材料
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包装工程
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Shuqi Guo,Qiang Zhao,Hongbo Hu,Wei Wang,Muhammad Bilal,Qiang Fei,Xuehong Zhang
Phenazine-1-carboxylic acid (PCA) secreted by Pseudomonas chlororaphis has been commercialized and widely employed as an antifungal pesticide. However, it displays potential hazards to nontarget microorganisms and the environment. Although the PCA degradation characteristics have received extensive attention, the biodegradation efficiency is still insufficient to address the environmental risks. In this study, an engineered Pseudomonas capable of degrading PCA was constructed by introducing heterologous PCA 1,2-dioxygenase (PcaA1A2A3A4). By integrating the PCA degradation module in the chemical mutagenesis mutant P3, 7.94 g/L PCA can be degraded in 60 h, which exhibited the highest PCA degradation efficiency to date and was 35.4-fold higher than that of the PCA natural degraders. Additionally, PCA was converted to 1-methoxyphenazine through structure modification by introducing the functional enzymes PhzSPa and PhzMLa, which has good antifungal activity and environmental compatibility. This work demonstrates new possibilities for developing PCA-derived biopesticides and enables targeted control of the impact of PCA in diverse environments.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xinxia Wang,Wei Yuan,Zhilan Sun,Fang Liu,Daoying Wang
Pseudomonas aeruginosa ( P. aeruginosa ) is an opportunistic infection-causing pathogen that threatens human health. Accordingly, a rapid and ultrasensitive analytical method is required urgently. Herein, an ultrasensitive multicolor electrochromic sensing platform was established on the basis of a closed bipolar electrode (BPE). Prussian blue (PB), the blue spot that can be easily electrodeposited, was selected as an electrochromic indicator at the closed BPE cathode. Integrating with the anodic emitter, Ru(bpy) 3 2+ , which emitted optical red light, visualized multicolor electrochromism was achieved on closed BPE. Particularly, physical separation between the positive and negative poles of the closed BPE greatly prevented mutual interference between Ru(bpy) 3 2+ and PB. Consequently, the sensitivity and accuracy of the proposed biosensor considerably improved. Notably, owing to magnetic-separation technology, the closed BPE surface required no modification. Without any complex pretreatment, the entire experiment time could be greatly shortened because the [email protected] completely captured P. aeruginosa in food matrix within only 20 min. By comparing the visual electrochromic colors, ultrasensitive screening of P. aeruginosa was accomplished within 1–10 8 CFU mL −1 . Combining the merits of closed BPE, electrochemiluminescence (ECL), and electrochromic, this strategy provided an accurate and intrinsic way for visual detection of P. aeruginosa , as well as great potential in measuring other pathogens.
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Frontiers in Chemistry
·
2022
·
IF 5.545
Zheng Hui,Huang Zhida,Chen Tongxin,Sun Yafeng,Chen Shouqing,Bu Guangming,Guan Hongcai
The continual resistance to antibiotics and the generation of a series of bacterial infections has emerged as a global concern, which requires appropriate measures and therapeutics to address such a menace. Herein, we report on Silk fibroin (SF) hydrogel with good biocompatibility and biodegradability fabricated through the crosslinking of the SF of different concentrations with Gallium nitrate (Ga (NO3)3) against Pseudomonas aeruginosa. However, the SF: Ga = 500: 1 (w/w) (SF/Ga) demonstrated a good bactericidal and wound healing effect as a result of the moderate and prolonged release of the Ga3+ following the gradual degradation of the hydrogel. The Ga3+, known for its innovative nature acted as a crosslinked agent and a therapeutic agent employing the “Trojan horse” strategy to effectively deal with the bacteria. Also, the Ga3+, which is positively charged neutralizes the negative potential value of the SF particles to reduce the charge and further induce the β-sheet formation in the protein structure, a characteristic of gelation in SF. The morphology showed a fabricated homogenous structure with greater storage modulus- G’ with low loss modulus- G'' modulus demonstrating the mechanical performance and the ability of the SF/Ga hydrogel to hold their shape, at the same time allowing for the gradual release of Ga3+. A demonstration of biocompatibility, biodegradability, bactericidal effect and wound healing in in vitro and in vivo present the SF/Ga hydrogel as an appropriate platform for therapeutic and for antibacterial wound dressing.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yanbo Wang,Yongzheng Wang,Jian Chen,Shigenobu Koseki,Qing Yang,Hejun Yu,Linglin Fu
Pseudomonas fluorescens and Shewanella baltica are specific spoilage organisms (SSOs) of most seafood products . Both of them can cell-to-cell communicate through quorum sensing (QS), facilitating the spoilage activities such as protease secretion, flagellar movement, and biofilm formation. Thus, it is important to screen the quorum sensing inhibitors (QSI) of P. fluorescens and S. baltica. In this study, we tried to apply a high-throughput virtual screening technology to select QSIs candidates. Three amino acid sequences from the key QS regulator–LuxR/I family (the LuxR and LuxI proteins of P. fluorescens, and the LuxR of S. baltica ) were targeted and analyzed . Their 3D structures were subsequently constructed for the QSIs candidates' screen of both P. fluorescens and S. baltica . The candidates of cytidine-5′-monophosphate (5′-CMP) and 5′-adenylic acid (5′-AMP) with high scores exerted strong antagonistic effects against both P. fluorescens and S. baltica QS. The addition of 5′-CMP and 5′-AMP also inhibit QS-controlled phenotypes (swimming motility, protease activity, EPS, and biofilm formation) in P. fluorescens and S. baltica . Furthermore, the exogenous addition of 5′-CMP and 5′-AMP could retard the spoilage process of salmon slices during low-temperature storage by inhibiting the production of extracellular protease, siderophores, and Total Volatile Basic Nitrogen (TVB-N).
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ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Gang Ni,Guangyu Shi,Chengxiao Hu,Xu Wang,Min Nie,Miaomiao Cai,Qin Cheng,Xiaohu Zhao
Most chemical plant wastewater contains both organic and inorganic pollutants , which are easy to diffuse along with surface runoff. The combined pollution of nonylphenol (NP) and cadmium (Cd) in soil is a serious problem that has not attracted enough attention. Based on the effects of selenium (Se) and Pseudomonas aeruginosa ( P. aeruginosa ) on plant and soil microbial communities , we speculated that the application of Se and P. aeruginosa in soil could improve the phytoremediation efficiency of ryegrass on contaminated soil. In this study, pot experiments with Cd and NP co-contaminated soil were conducted, and the results showed that application of P. aeruinosa alone could improve the removal rates of NP and Cd by ryegrass, and the supplementary of Se further enhanced the effect of micro-phyto remediation, with the highest removal rates of NP and Cd were 79.6% and 49.4%, respectively. The application of P. aeruginosa plus Se reduced the adsorption of Cd and NP through C–O and Si–O–Fe of the soil, changed the enzyme activity , and also affected the changing trend of the microbial community in soil. Pseudomonas , Sphingomonadales , Nitrospira , and other beneficial bacteria were enriched after a 60-day period with P. aeruginosa and Se treatment, thus promoting the removal of NP and Cd. In light of the above results, we suggest that P. aeruginosa application can efficiently facilitate the phytoremediation of ryegrass on Cd-NP co-contaminated soil, and Se supplementation in soil showed the synergistic effect on the remediation.
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Catalysts
·
2021
·Vol. 11
·期 10
·1214
·
IF 4.146
Weilai Fu,Qiang Wang,Shuhui Chen,Yunshuang Wang,Yaru Wang,Peifeng Duan,Ganfeng Yi,Chao Liu,Xian Zhang,Zhiming Rao
Nitrogen pollution in water bodies is becoming increasingly serious, and how to remove nitrogen from water bodies economically and effectively has become a research hotspot. Especially in recent years, with the gradual expansion of aquaculture in China, the content of nitrite and other nitrogen-containing substances in water bodies has been increasing, which inhibits the growth of farm animals and is one of the causes of eutrophication in water bodies. In this study, a strain of bacteria was isolated from the sludge of an aquaculture fishpond and identified asPseudomonas stutzeri, which can efficiently degrade nitrite. After continuous domestication in nitrite mixed solution, the nitrite nitrogen reduction capacity ofP. stutzeriwas significantly improved. Univariate experiments aiming to optimize the degradation conditions indicate that the optimal culture conditions for strain F2 are: medium with a carbon source of sodium succinate; C/N of 18; pH of 8; culture temperature of 28 °C; and shaking speed of 210 rpm in the shaker. Under the optimal culture conditions, the NO2−-N concentration of the culture solution was 300 mg/L, and the nitrite removal rate reached 98.67%. Meanwhile, the results of the nitrogen balance test showed that the strain converted 6.1% of the initial nitrogen into cellular organic nitrogen and 62.3% into gaseous nitrogen.Keywords:nitrogen pollution;nitrite removal;domestication;Pseudomonas stutzeri
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ACS Applied Nano Materials
·
2025
·
IF 5.5
Jinmin Wang,Chenkai Chu,Mingming Fang,Yongxin Tao,Yong Qin,Zheng Liang
The structure of covalent organic framework nanosheets (COF NSs) effectively mitigates fluorescence quenching caused by π–π stacking. Designing functionalized COF NSs as luminescent materials represents an innovative approach to expanding research on COF-based fluorescence sensing. Given that COF NSs expose a greater number of binding sites, they can be functionalized with appropriate luminescent elements to form a COF NS composite. The interaction between analytes and COF NS composites results in significant changes in luminescence, making these composites promising candidates for chemosensing applications. In this study, we introduce functionalized dual luminescent centers (silicon quantum dots (SiQDs) and Eu3+ complexes) into COF skeletons via a modification-facilitated exfoliation strategy. This process transforms the bulk COF (MPCOF) into a COF NS composite (1) with an enhanced luminescent performance. As a fluorescent sensor, 1 detects the biomarker 2-aminoacetophenone (2-AA) from Pseudomonas aeruginosa using ratiometric signals, facilitating visual sensing analysis. Leveraging its high sensitivity, an algorithmic method based on deep learning has been established to predict the fluorescence response of sensor 1 to specific concentrations of 2-AA. This facile determination of 2-AA enables effective monitoring of human or animal health and evaluation of water environments.
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Water
·
2024
·Vol. 16
·期 9
·1302
·
IF 3
Jinfeng Zhao,Huichao Zhu,Chen Tao,Zhiquan Wang,Ning Deng,Xin Huang
Conventional disinfection techniques, relying on a single disinfection step, often fail to directly eliminate microorganisms, instead causing them to enter a viable but nonculturable (VBNC) state. However, microorganisms in the VBNC state retain metabolic activity and can reactivate under suitable conditions, representing a “hidden source of contamination” that threatens drinking water safety. This study fundamentally assessed the feasibility of combined disinfection methods by integrating UV254with disinfectant (NaClO, PAA, and PDS) for inactivatingPseudomonas aeruginosa(P. aeruginosa), an opportunistic pathogen that has been widely detected in water supply systems. The number of culturable cells was determined using the heterotrophic plate counting (HPC) method, and the number of VBNC cells was quantified using our recently developed qPCR approach. Quantitative analyses showed that combined disinfection methods can effectively reduce both culturable and VBNC cells by several orders of magnitude compared to a single disinfection step. Notably, VBNCP. aeruginosa,after 30 min of UV/NaCIO treatment, was below the detection limit (3.191 log CFU/mL) of PMA-qPCR. The reactivation experiment also confirmed that VBNCP. aeruginosadid not reactivate for 16 h after 30 min of UV/NaClO treatment under controlled laboratory conditions. The higher disinfection capacity of combined methods can be attributed to the generation of reactive radicals. This study highlighted combined disinfection as a promising approach for the inactivation of bacteria in the VBNC state, yet further studies are needed before an application can be considered for minimizing VBNC reactivation in city utility water processing or high-risk building water distribution systems.
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ACS Materials Letters
·
2024
·
IF 9.6
Xiaoliang Qi,Yizuo Shi,Chaofan Zhang,Erya Cai,XinXin Ge,Yajing Xiang,Ying Li,Bairui Zeng,Jianliang Shen
Contemporary options for multidrug-resistant bacteria infected diabetic foot ulcers (IDFUs) are predominantly nonspecific. These IDFU injuries often display prolonged inflammation and delayed tissue repair, mainly attributed to an overabundance of M1 macrophages in the hostile diabetic microenvironment. Although immunomodulatory hydrogels show promise for IDFU-focused care, a targeted, safe transition from M1 to M2 macrophages using simplified techniques remains a significant obstacle. Here, we introduce a hybrid hydrogel (GGG) with inherent immunomodulatory capabilities for IDFUs. GGG is composed of interpenetrating polymer networks featuring gallium-induced self-assembling glycyrrhizic acid and a photo-cross-linked gelatin methacryloyl matrix. Importantly, GGG effectively decreases the ratio of M1 to M2 macrophages in conditions infected with drug-resistant bacteria by disrupting iron metabolism and scavenging reactive oxygen species, which contributes to enhanced treatment results for diabetic wounds. To sum up, the immunomodulatory strategy we present provides a straightforward, safe, and highly effective therapeutic avenue for managing IDFUs.
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Microorganisms
·
2024
·Vol. 12
·期 4
·841
·
IF 4.1
Chaodong Song,Rui Liu,Doudou Yin,Chenjie Xie,Ying Liang,Dengfeng Yang,Mingguo Jiang,Hongyan Zhang,Naikun Shen
Microbial degradation of feathers offers potential for bioremediation, yet the microbial response mechanisms warrant additional investigation. In prior work,Pseudomonas aeruginosaGxun-7, which demonstrated robust degradation of feathers at elevated concentrations, was isolated. However, the molecular mechanism of this degradation remains only partially understood. To investigate this, we used RNA sequencing (RNA-seq) to examine the genes that were expressed differentially inP. aeruginosaGxun-7 when exposed to 25 g/L of feather substrate. The RNA-seq analysis identified 5571 differentially expressed genes; of these, 795 were upregulated and 603 were downregulated. Upregulated genes primarily participated in proteolysis, amino acid, and pyruvate metabolism. Genes encoding proteases, as well as those involved in sulfur metabolism, phenazine synthesis, and type VI secretion systems, were notably elevated, highlighting their crucial function in feather decomposition. Integration of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) taxonomies, combined with a review of the literature, led us to propose that metabolic feather degradation involves environmental activation, reducing agent secretion, protease release, peptide/amino acid uptake, and metabolic processes. Sulfite has emerged as a critical activator of keratinase catalysis, while cysteine serves as a regulatory mediator. qRT–PCR assay results for 11 selected gene subset corroborated the RNA-seq findings. This study enhances our understanding of the transcriptomic responses ofP. aeruginosaGxun-7 to feather degradation and offers insights into potential degradation mechanisms, thereby aiding in the formulation of effective feather waste management strategies in poultry farming.
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