ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Yi Liang,Xiaolin Huang,Ruijin Yu,Yaofeng Zhou,Yonghua Xiong
The present study described a novel fluorescence enzyme-linked immunosorbent assay (ELISA) used to detect ochratoxin A (OTA) by using the glucose oxidase (GOx)-mediated fluorescence quenching of mercaptopropionic acid-capped CdTe quantum dots (MPA-QDs), in which GOx was used as an alternative to horseradish peroxidase (HRP) for the oxidization of glucose into hydrogen peroxide (H 2 O 2 ) and gluconic acid . The MPA-QDs were used as a fluorescent signal output, whose fluorescence variation was extremely sensitive to the presence of H 2 O 2 or hydrogen ions in the solution. Under the optimized conditions, the proposed fluorescence ELISA demonstrated a good linear detection of OTA in corn extract from 2.4 pg mL −1 to 625 pg mL −1 with a limit of detection of 2.2 pg mL −1 , which was approximately 15-fold lower than that of conventional HRP-based ELISA. Our developed fluorescence immunoassay was also similar to HRP-based ELISA in terms of selectivity , accuracy, and reproducibility. In summary, this study was the first to use the GOx-mediated fluorescence quenching of QDs in immunoassay to detect OTA, offering a new possibility for the analysis of other mycotoxins and biomolecules.
研究领域:
食品科学
·
分析化学
·
毒理学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2013
·Vol. 14
·期 4
·8603-8610
·
IF 2.464
Hua Kuang,Gang Cui,Xiujin Chen,Honghong Yin,Qianqian Yong,Liguang Xu,Chifang Peng,Libing Wang,Chuanlai Xu
Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detectSalmonellawith sensitivity of 500 cfu mL−1. With two monoclonal antibodies, which recognize different antigenic determinant on the surface ofSalmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency forSalmonella(90%). A sandwich structure formed if theSalmonellasolution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount ofSalmonella. A linear response between fluorescence intensity and various concentrations ofSalmonella(2.5 × 103to 1.95 × 108cfu mL−1) were observed with this proposed method. The total assay time forSalmonellawas 30 min, and no cross-reaction to other microbial strains, such asStaphylococcus aureus,Escherichia coli(E. coli) andEscherichia coli O157:H7(E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied inSalmonellascreening without time-consuming extra-enrichment of bacteria.Keywords:Salmonella;fluorescent immunoassay;magnetic separation;quantum dots
研究领域:
生物技术
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微生物学
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纳米技术
·
食品安全
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DOI
PubMed
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Yuwei Liang,Chunling Hu,Peng Chen,Qi Li,Jianning Wu,Zhiyong Liu,Jichang Liu,Yanlong Tai
5-hydroxymethylfurfural (HMF) can catalyte into 2,5-diformylfuran (DFF) which had received much attention as a potential biomass resource. Here, we used a simple and effective method to construct hollow CdS quantum dot (CdS QDs)/MIL-101 heterostructures with high contact area and multiple active sites. The structure of heterostructures could improve photocatalytic activity by charge transfer, photogenerated carrier separation and the multiple refractions of light supported within the hollow-structured CdS QDs. The detailed catalytic mechanism was illustrated by trap agent experiments and electron paramagnetic resonance (EPR) spectroscopy, in which superoxide radicals (·O 2 - ) play a decisive role in the catalytic oxidation of HMF to DFF. Furthermore, the large specific surface area of MIL-101 adsorbs O 2 from the reaction system thereby accelerating the ·O 2 - formation process. Under environmental conditions, 70 % CdS QDs/MIL-101 exhibited optimal catalytic performance. Comparing with pure CdS QDs, the photocurrent enhances 1.8 times, HMF conversion is 1.5 times and DFF selectivity is 1.2 times. This work provide a useful way to improve the conversion of biomass by Z-heterostructures under ambient conditions.
研究领域:
化学工程
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材料科学
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环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qianfeng Li,Jian Sun,Xiang Li,Changling Yan,Gongke Wang
Silver ion (Ag + ) is a significant metal pollutant known for its potential toxicity to aquatic life and human health. Long-term exposure to toxic heavy metals will do great harm to humans and animals. Therefore, it is urgent to develop an efficient method for detecting Ag + . Herein, we have developed a simple, sensitive, highly selective and cost-effective fluorescence “off-on” mode nanosensor based on environmentally friendly and biocompatible materials for sensitive detection of Ag + in aqueous environments. In this sensing strategy, Ag + can specifically interact with the cytosine-cytosine (C-C) mismatch in single-stranded DNA, where Ag + induces the folding of single-stranded DNA to form hairpin structures and stable metal-mediated cytosine-Ag + -cytosine (C-Ag + -C) base pairs via coordinate bonds. Massive changes to the molecular volume and fluorescence signal are brought about by the development of the C-Ag + -C complex. To reach our objective, CdTe quantum dots (CdTe QDs) with low toxicity and strong signal were prepared as fluorophores for detecting Ag + target. Using the inner filter effect (IFE) between hydrophilic CdTe QDs and MoS 2 nanosheets to achieve the fluorescence quenching of the CdTe QDs, and the subsequent fluorescence turn-on process is due to the formation of C-Ag + -C complex, which makes its probe fluorophore far away from the surface of MoS 2 nanosheets, thus realizing fluorescence “off-on” sensing strategy. The proposed nanosensor can distinguish Ag + from other interfering ions with high sensitivity and selectivity. The nanosensor proposed here possesses a quick response time of 8 min and an ultra-low detection limit of 1.1 nM.
研究领域:
环境科学
·
生物化学
·
材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yingwang Ye,Tingting Wu,Xiuting Jiang,Jinxuan Cao,Xiao Ling,Qingsong Mei,Hua Chen,Deman Han,Jing-Juan Xu,Yizhong Shen
Accurate onsite profiling of fluoroquinolone antibiotics (FQs) is of vital significance for ensuring food safety and estimating environmental pollution. Here, we propose a smartphone-based QD ratiometric fluorescence-sensing system to precisely report the level of FQs. As a proof of concept, we chose gatifloxacin (GFLX, a typical member of FQs) as the model for the analytical target, which could effectively trigger the fluorescence color variation of QDs from bright yellow-green (∼557 nm) to blue (∼448 nm) through the photoinduced electron-transfer (PET) process, thus yielding an evident ratiometric response. Based on this, the level of GFLX can be reported within a wide linear range from 0.85 nM to 3.6 μM. Moreover, this assay owns a high sensitivity with a low detection limit of 0.26 nM for GFLX and a quick sample-to-answer monitoring time of 5.0 min, manifesting that this platform could be fully qualified for onsite requirements. Interestingly, this portable device has successfully been applied for the onsite detection of GFLX in real food (i.e., milk and drinking water) and environmental (i.e., fish-farming water) samples with acceptable results. This developed platform offers a great promise for the point-of-care detection of FQ residues in practical application with the merits of being label-free, low-cost, and rapid, thus opening a new pathway for the onsite evaluation of food safety and environmental health.
研究领域:
分析化学
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环境科学
·
食品科学
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DOI
高影响因子
Science Bulletin
·
2025
·
IF 21.1
Tianjun Hu,Xiuting Wu,Qingsong Shan,Xingxing Duan,Xiaoyue Cai,Mengjin Huang,Junhui Dai,Hong Zhong,Beichen Yuan,Hengyang Xiang,Linxiang Yang,Qiangyu Dai,Yuhao Zhang,Jiangshan Chen,Haibo Zeng
Developing single-pixel color-tunable electroluminescence technology represents a practical approach to circumvent current limitations in pixel processing and longevity. However, existing single-pixel color-tunable quantum dot light-emitting diodes (QLEDs) devices predominantly employ complex tandem structures. Herein, we demonstrate single-layer color-tunable QLEDs by the real-time interface exciplex modulation with AgInZnS (AIZS) quantum dots (QDs). The dynamically color-tunable QLEDs based on green- and red-emitting AIZS QDs exhibit broad spectral coverage, spanning from green to blue, red to yellow, with standard white emission, respectively. The fabricated devices achieve a leading maximum external quantum efficiency of 5% for quaternary alloy AIZS-based QLEDs. Furthermore, we successfully demonstrate patterned and large-area QLEDs, systematically confirming their significant potential for application in full-color display and lighting technologies.
研究领域:
光电子学
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量子点技术
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显示技术
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发光二极管
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 14
·12045-12054
·
IF 2.324
Huang Zhaohui,Chen Hui,Zhao Lei,He Xuan,Fang Wei,Du Yingying,Li Weixin,Wang Guanghui,Zhang Fuqing
With increasing concern about the problems of environmental pollution and energy, the visible light photocatalytic for dye degradation is receiving considerable attention. CdSe quantum dot (CdSe QDs) sensitized MIL-125/TiO 2 @SiO 2 biogenic hierarchical materials (CMTS) has been fabricated through dual modification of CdSe with TiO 2 and MIL-125, that is the metal–organic framework (MOF) with Ti ions as metal centers and terephthalic acid as the organic ligand. The hierarchical porous heterostructured MIL-125/TiO 2 @SiO 2 composites are firstly obtained by a biogenic-templated method using rice husk as biomorphic. CdSe QDs as sensitizer is further deposited on MIL-125/TiO 2 @SiO 2 via a dipping method. This strategy applies CdSe, MIL-125, and TiO 2 undergo a two-level heterostructure, setting a stage for efficient charge separation. In addition, CMTS possesses a hierarchical porous structure in scale from micrometer to nanometer with high BET surface area. As a result, the CMTS can efficiently adsorption target, improving light harvesting and promoting degradation of organic waste from water. Meanwhile, Rhodamine B aqueous solution can be degraded more than 98% by CMTS in 100 min under visible light irradiation.
研究领域:
材料科学
·
环境科学
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DOI
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Lu Zhang,Kun Wang,Feng Zhou,Yuwei Bu,Xiaoyan Yang,Guangming Nie
A sensitive photoelectrochemical (PEC) biosensor for silver ions (Ag+) was developed based on Zn-Co doped C and CdS quantum dots (CdS QDs) nanomaterials. Hydrophobic modified sodium alginate (HMA), which could stabilize and improve the PEC performance of CdS QDs, was also used for the construction of PEC sensors. Specially, Zn-Co doped C, CdS QDs and HMA were sequentially modified onto the electrode surface via the drop-coated method, and the C base rich DNA strand was then immobilized onto the modified electrode. As the C base in DNA specifically recognized Ag+, it formed the C-Ag+-C complex in the presence of Ag+, which created a spatial steric hindrance, resulting in a reduced PEC response. The sensing platform is sensitive to Ag+ in the range of 10.0 fM to 0.10 µM, with a limit of detection of 3.99 fM. This work offers an ideal platform to determine trace heavy metal ions in environmental monitoring and bioanalysis.
研究领域:
环境科学
·
生物分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Shuai Zhang,Hongli Liu,Xianggao Li,Shirong Wang
Inorganic perovskite quantum dots (QDs) have emerged as promising materials for light-emitting devices by merits of their high photoluminescence quantum yield (PLQY), narrow full width at half-maximum and tunable emission spectrum. However, fluorescence quenching and trap-mediated nonradiative recombination between hole transport layer (HTL) and QD films are prejudicial to the performance of perovskite-QDs-based light-emitting devices (QLEDs). Here, HTL was modified with diamine molecules (1,4-butanediamine (4-DA), 1,8-octyldiamine (8-DA) and 1,11-aminoundecylamine (11-DA)) through a simple self-assembly method. QD films based on modified poly[ N , N ′-bis(4-butylphenyl)- N , N ′-bis(phenyl)-benzidine] (Poly-TPD) exhibited higher PL intensity and lower fluorescence quenching properties. Meanwhile, diamine molecules do favor to the flatness of Poly-TPD film and contribute to the smoothness of QD films. Moreover, the exciton quenching and the density of defects at the interface between HTL and QD films were effectively reduced. Particularly, QLEDs based on HTL modified with 8-DA was endowed with a maximum luminance of 78634 cd/m 2 , a peak current efficiency of 30.77 cd/A, a maximum external quantum efficiency of 8.90% and T 50 of 16.8 min when the initial brightness is about 1000 cd/m 2 .
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 32
·7160-7165
·
IF 5.344
Tao Hu,Kangkai Xu,Shanhu Qiu,Yu Han,Juan Chen,Jian Xu,Ke Chen,Zilin Sun,Hong Yi,Zhonghua Ni
A sensitive fluorescent microfluidic sensor based on carbon quantum dots (CQDs), cadmium telluride quantum dots (CdTe QDs) aerogel and glucose oxidase (GOx) for urinal glucose detection was fabricated via a simple method. CQDs, emitting green fluorescence (emission peak at 520 nm) and the CdTe QDs, emitting red fluorescence (emission peak at 620 nm) under 365 nm ultraviolet irradiation, were synthesized. The as-prepared CQDs, CdTe QDs and GOx (C/CdTe QD-GOx) were mixed in a certain ratio and were then introduced into the microfluidic chip to construct a three-dimensional porous aerogel sensor after freeze-drying. A ratiometric fluorescence response was realized through the quenching of the red fluorescence by H2O2 produced from the glucose/GOx reaction, while the green fluorescence intensity stays constant. A small change in the ratio of the two fluorescence intensities led to a clear change in the fluorescent colour of the sensor, which can be easily observed under a UV lamp. The microsensor displayed a serial colour change from red to green with the changes in glucose concentration. The intensity ratio between the red fluorescence and green fluorescence (R/G value) indicates the glucose concentration. In our experiments, the optimal concentration ratio of CdTe QDs and CQDs was 0.5 : 1, and the optimal response time was 5 min. The detection range of glucose concentration was from 0 mM to 13 mM, and the detection limit was 0.223 mM. We also found that this glucose sensor has excellent selectivity, specificity and stability. The as-prepared microsensor can be stably stored for 30 days under −20 °C. Such a C/CdTe QD-GOx aerogel based microfluidic assay sensor provides a new approach towards the optical and quantitative detection of glucose, which enables daily urine glucose monitoring for the diabetic patients in a convenient way.
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Wenfei Liang,Linping Zhong,Lijun Quan,Xuejia Wang,Mengjiao Liu,Xin Lai,Jian Bi,Daojiang Gao,Xiaohong Liu,Wei Zhang,Yan Zhao
Transition metal oxides (TMOs) based compounds are recognized as high-energy anodes in next-generation lithium-ion batteries (LIBs) due to their extremely high theoretical capacity. However, the lower intrinsic conductivity , fast fading cyclic stability and dramatic volume expansion during discharge/charge processes greatly hinder the practical application of TMOs as anodes in LIBs. In this study, we have designed and constructed MoO 3 /ZnCo 2 O 4 QDs@C@rGO/MoO 3 (denoted as GMZCO) sandwich-structured hybrid nanocomposites via an effective route, and have proposed the formation mechanism of this unique nanostructured composite on the basis of the systematic results of the microstructures. Due to the unique sandwich structure and the synergistic enhancement effect of the ZnCo 2 O 4 QDs , α-MoO 3 and rGO, the as-constructed GMZCO exhibits the superior electrochemical performances when used as anode for LIBs. The obtained GMZCO can maintain high capacity of 1281 mAh g −1 at 0.2 A g −1 after 300 cycles, and can deliver as high as specific capacity of 913 mAh g −1 at 1 A g −1 after 500 cycles, revealing the higher capacity and excellent cyclic stability. What deserves to be mentioned most is that GMZCO can keep a capacity of 541 mAh g −1 after 1400 cycles even at higher current density of 3 A g −1 , having outstanding rate capability. This work paves a novel approach for design TMOs with unique microstructures as a promising anode for the high-performances LIBs.
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DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1395-1400
·
IF 1.938
Li Wang,Di Luo,Dongdong Qin,Duoliang Shan,Xiaoquan Lu
This paper reports the electrochemiluminescence (ECL) behavior of CdSe/ZnS with K2S2O8 as the coreactant. The effects of pH, K2S2O8 concentration, the duration of the reaction of 4-ATP with GCE and the duration of the reaction of 4-ATP/GCE with QDs on ECL intensity were studied in detail. A possible ECL reaction mechanism was then proposed. In addition, it was observed that the ECL intensity was efficiently quenched by trace amounts of heavy metal ion. Based on the quenching effect of Pb2+ upon the ECL of CdSe/ZnS QDs, a QDs-ECL sensor was constructed to detect Pb2+ in preserved duck egg. The sensor showed good reproducibility and stability.
研究领域:
分析化学
·
电化学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）