Regenerative Biomaterials
·
2024
·
IF 5.6
Diao Zhentian,Li Longkang,Zhou Huan,Yang Lei
Healing of chronic diabetic wounds is challenging due to complications of severe inflammatory microenvironment, bacterial infection and poor vascular formation. Herein, a novel injectable polyvinyl alcohol–hyaluronic acid-based composite hydrogel was developed, with tannic acid (TA) and silicate functionalization to fabricate an ‘all-in-one’ hydrogel PTKH. On one hand, after being locally injected into the wound site, the hydrogel underwent a gradual sol–gel transition in situ, forming an adhesive and protective dressing for the wound. Manipulations of rheological characteristics, mechanical properties and swelling ability of PTKH could be performed via regulating TA and silicate content in hydrogel. On the other hand, PTKH was capable of eliminating reactive oxygen species overexpression, combating infection and generating a cell-favored microenvironment for wound healing acceleration in vitro. Subsequent animal studies demonstrated that PTKH could greatly stimulate angiogenesis and epithelization, accompanied with inflammation and infection risk reduction. Therefore, in consideration of its impressive in vitro and in vivo outcomes, this ‘all-in-one’ multifunctional hydrogel may hold promise for chronic diabetic wound treatment.
研究领域:
生物材料
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医学工程
·
再生医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Jiang Yaqiang,Zhang Zhaoguo,Xuan Chengkai,Shi Xuetao
Effective wound management is critical in post-operative recovery, particularly in sensitive areas such as circumcision. The aim of this study was to design and assess the efficacy of a novel two-component polyurethane (PU) bioadhesive, designated as PU1000S, for its application in advanced wound care within the context of clinical circumcision procedures. The glue-type bioadhesive was fine-tuned to conformally adhere to the moist tissue surfaces. It rapidly absorbed interfacial water and cured within 160 s, ensuring remarkable surface adaptability to wet tissue surfaces. The PU1000S demonstrated superior lap-shear strength, peaking at 55.12 ± 6.88 kPa, along with exceptional durability. These attributes underscored its strong wet adhesion and remarkable resilience at the interface with moist tissues. Owing to its mechanical adaptability to wet skin tissue, the adhesive layer of PU1000S maintained stability under complex loading associated with twisting, folding and significant volumetric deformation, resulting in minimal debonding. In addition, PU1000S was found to significantly accelerate wound healing by promoting re-epithelialization and collagen deposition, confirming its excellent bioadaptability for initial closure and subsequent tissue repair and regeneration following circumcision. The comprehensive results position PU1000S as a promising candidate for advanced wound care in circumcision, offering superior performance in terms of wet adhesion, durability, and bioadaptability. Its application could potentially enhance clinical outcomes and elevate patient satisfaction.
研究领域:
生物医学工程
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材料科学
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临床医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Zhou Xiaosong,Guo Min,Wang Zongliang,Wang Yu,Zhang Peibiao
Bioactive microspheres coated with acellular matrix (aECM) have received extensive attention in bone tissue engineering. In this work, biomimetic microspheres with different aECM ratios, uniform size and controllable size were prepared easily by blending natural porcine dermal aECM and poly (lactic-co-glycolic acid) (PLGA) using electrohydrodynamic spraying and solidification actuated by solvent extraction (ESASE) method. In this work, the appropriate polymer concentration and preparation voltage were investigated, and the surface morphology of the microspheres were observed by scanning electron microscope. Sirius red was used to visualize aECM exposure on the surface of the microspheres. The in vitro and in vivo experiments was carried out to evaluate the bioactivity and osteogenic properties of the microspheres. The results showed that the morphology and size of PLGA microspheres had little influence on the aECM blending. In vitro experiments showed that the higher the content of aECM, the better the cell adhesion performance. In vivo, rat calvarial defect models were observed and characterized at 4 and 8 weeks postoperatively, and the values of BV/TV of 50aECM/PLGA were 47.57 ± 1.14% and 72.92 ± 2.19%, respectively. The results showed that the skull healing effect was better in aECM-containing microspheres. In conclusion, aECM/PLGA composite microspheres can increase cell adhesion performance through the addition of aECM. Moreover, in vivo experiments have proved that aECM/PLGA microspheres is beneficial to bone repair, which means the aECM/PLGA microspheres is a promising bone tissue engineering material.
研究领域:
组织工程
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生物材料
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生物医学工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Zou Weilong,Han Xiyuan,Dai Qiyuan,Lin Zequ,Li Qingtao,Li Zilin,Xu Xinrong,Chen Xinying,Gao Huichang,Cao Xiaodong
Natural bone is a naturally mineralized material with a non-homogeneous porous structure, which is difficult to construct using conventional manufacturing methods. Triply periodic minimal surfaces (TPMS) have emerged as an excellent solution in recent years for constructing porous artificial bone structures, characterized by smooth surfaces, highly interconnected porous structures, and mathematically controllable geometries. In this work, digital light processing (DLP) printing technology was used to construct a non-homogeneous TPMS structure with strontium-doping 13-93 bioactive glass (Sr@BG) prepared by fusion method. The heterogeneous scaffolds were obtained by integrating high-strength I-wrapped package (I) and high-permeability Gyroid (G) units behaving a sufficient compressive strength of 5.8±0.6 MPa, a porosity of ∼63% and a permeability of 0.97 × 10−8 m2, which matched the microstructural parameters of cancellous bone. Meanwhile, the biomimetic structure and Sr doping could cooperatively promote the adhesion, proliferation and differentiation of bone mesenchymal stem cells (BMSCs). In addition, the osteogenic ability of IG scaffolds was verified in rabbit’s femoral condylar defect. In general, heterogeneous IG scaffolds possess desirable bioactivity and mechanical property which meet the functional and structural requirements of bone regeneration
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Yang Shuaikang,Wang Weiqiang,Xu Yanan,Yuan Yonghui,Hao Shengzhi
Fe-based biodegradable materials have attracted significant attention due to their exceptional mechanical properties and favorable biocompatibility. Currently, research on Fe-based materials mainly focuses on regulating the degradation rate. However, excessive release of Fe ions during material degradation will induce the generation of reactive oxygen species (ROS), leading to oxidative stress and ferroptosis. Therefore, the control of ROS release and the improvement of biocompatibility for Fe-based materials are very important. In this study, new Fe–Zn alloys were prepared by electrodeposition with the intention of using Zn as an antioxidant to reduce oxidative damage during alloy degradation. Initially, the impact of three potential degradation ions (Fe2+, Fe3+, Zn2+) from the Fe–Zn alloy on human endothelial cell (EC) activity and migration ability was investigated. Subsequently, cell adhesion, cell activity, ROS production and DNA damage were assessed at various locations surrounding the alloy. Finally, the influence of different concentrations of Zn2+ in the medium on cell viability and ROS production was evaluated. High levels of ROS exhibited evident toxic effects on ECs and promoted DNA damage. As an antioxidant, Zn2+ effectively reduced ROS production around Fe and improved the cell viability on its surface at a concentration of 0.04 mmol/l. These findings demonstrate that Fe–Zn alloy can attenuate the ROS generated from Fe degradation thereby enhancing cytocompatibility.
研究领域:
材料科学与工程
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细胞生物学
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医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Xie Yinhong,Chi Mengchen,Yang Xinlei,Dong Ruichen,Yang Ao,Yin Antao,Weng Yajun
Modification of polylactic acid (PLA) is a promising strategy for the next generation of bioresorbable vascular stent biomaterials. With this focus, FeMOFs nanoparticles was incorporated in PLA, and then post loading of CO was performed by pressurization. It showed FeMOFs incorporation increased hydrophilicity of the surface and CO loading, and CO release was sustained at least for 3d. It is well acknowledged NETosis and macrophage mediated inflammation are the principal effectors of atherosclerosis and cardiovascular disease, and it further increases the risk of late stent thrombosis and restenosis. In this study, the effects of CO release of PLA/FeMOFs/CO on NETosis and macrophage behavior were thoroughly explored. In vitro evaluation results showed that PLA/FeMOFs/CO significantly inhibited neutrophil extracellular traps (NETs) release and neutrophil elastase (NE) expression by reducing intracellular ROS in a simulated inflammatory environment. It reduced LPS-induced macrophage inflammation with decreased TNF-α expression and increased IL-10 expression. Meanwhile it enhanced endothelial cell activity and growth in inflammatory environment, and inhibited platelet adhesion and activation. In vivo implantation results confirmed that PLA/FeMOFs/CO reduced the macrophages and neutrophils mediated inflammatory response, thus reduced the neointimal hyperplasia. Overall, PLA/FeMOFs/CO effectively prevented the inflammation and restenosis associated with PLA implantation. Our study provides a new strategy to improve the immunocompatibility of PLA implant materials.
研究领域:
生物材料
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心血管工程
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免疫学
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组织工程
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Wu Chunwei,Zhong Jie,Li Jianing,Luo Yande,Wang Junyao,Zeng Xiaodie,Mao Jiaji,Lu Jianping,Xu Junyao,Wu Changqiang,Wang Zhiyong
To address the limitations of low relaxivity and physiological toxicity in commercial gadolinium-based contrast agents for magnetic resonance imaging (MRI), a novel manganese chelate macromolecular system was developed using a flash nano-preparation technique. Herein, the approach applying an instantaneous fluid device incorporated gallic acid, dopamine and Mn2+ to perform in-situ polymerization of dopamine and covalent binding with albumin in a nanoconfined environment. This controllable self-assembly process characterized by its scalability and reproducibility was suitable for industrial-scale production. Under optimized flow rates and material ratios, the synthesized ultrasmall protein-based system, Mn-GA@BSA@DA, exhibited excellent aqueous dispersion with an average size of approximately 18 nm, allowing for long-term lyophilized powder storage. More importantly, the nanosystem demonstrated superior MRI-T 1 relaxivity, significantly surpassing that of clinical Gadopentetate dimeglumine, with a high value around 18.5 mM−1 s−1 and a low r 2/r 1 ratio (< 5 at 3.0 T). Furthermore, this Mn-GA@BSA@DA contrast agent was endowed with tumor-targeting effects and long MRI monitoring window period for the liver, gallbladder and renal tubules. The metal chelation within the nanoagent minimizes Mn2+ release; importantly, the antioxidant components, gallic acid and dopamine, significantly inhibit the Fenton reaction-induced toxicity, enhancing biocompatibility. Therefore, this study presents a simple and scalable production technique for a kind of MRI-T 1 weighted contrast agent with high relaxivity and biocompatibility, offering a promising alternative to commercial Gd chelates.
研究领域:
医学影像学
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材料科学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Liu Xin,Li Jiale,Liu Shuyu,Long Yan,Kang Ching,Zhao Chen,Wei Ling,Huang Shaoqi,Luo Yi,Dai Bo,Zhu Xiangjia
Posterior capsule opacification (PCO) remains the predominant complication following cataract surgery, significantly impairing visual function restoration. In this study, we developed a PCO model that closely mimics the anatomical structure of the crystalline lens capsule post-surgery. The model incorporated a threaded structure for accurate positioning and observation, allowing for opening and closing. Utilizing 3D printing technology, a stable external support system was created using resin material consisting of a rigid, hollow base and cover. To replicate the lens capsule structure, a thin hydrogel coating was applied to the resin scaffold. The biocompatibility and impact on cellular functionality of various hydrogel compositions were assessed through an array of staining techniques, including calcein-AM/PI staining, rhodamine staining, BODIPY-C11 staining, and EdU staining in conjunction with transwell assays. Additionally, the PCO model was utilized to investigate the effects of eight drugs with anti-inflammatory and anti-proliferative properties, including 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), THZ1, sorbinil, 4-octyl itaconate (4-OI), xathohumol, zebularine, rapamycin, and caffeic acid phenethyl ester (CAPE), on human lens epithelial cells (HLECs). Confocal microscopy facilitated comprehensive imaging of the PCO model. The results demonstrated that the GelMA 60 5% + PLMA 2% composite hydrogel exhibited superior biocompatibility and minimal lipid peroxidation levels among the tested hydrogels. Moreover, compared to using hydrogel as the material for 3D printing the entire model, applying surface hydrogel spin coating with parameters of 2000 rpm x 2 on the resin-based 3D printed base yielded a more uniform cell distribution and reduced apoptosis. Furthermore, rapamycin, 4-OI, and AICAR demonstrated potent antiproliferative effects in the drug intervention study. Confocal microscopy imaging revealed a uniform distribution of HLECs along the anatomical structure of the crystalline lens capsule within the PCO model, showcasing robust cell viability and regular morphology. In conclusion, the PCO model provides a valuable experimental platform for studying PCO pathogenesis and exploring potential therapeutic interventions.
研究领域:
眼科学
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生物材料学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Meng Ruo-Yin,Xia Hong-Ying,Zhao Ying,Ye Ying-Tong,Wang Shi-Bin,Chen Ai-Zheng,Kankala Ranjith Kumar
Triple-negative breast cancer (TNBC) has emerged as one of the dreadful metastatic tumors in women due to complexity, specificity, and high recurrence, resulting in poor therapeutic outcomes and requiring real-time monitoring for improved theranostics. Despite the success as efficient radiosensitizers and computed tomography (CT)-based contrast agents, bismuth (Bi)-based composites suffer from poor colloidal stability, dose-dependent toxicity, and pharmacokinetic shortcomings, leading to poor therapeutic monitoring. In addition, several small molecule-based therapeutics, including nanoparticle-based delivery systems, suffer from several limitations of poor therapeutic delivery and acquired multidrug resistance by cancer cells, depriving the therapeutic needs. To overcome this aspect, this study demonstrates the fabrication of drug-like/drugless nanoarchitectures based on copper sulfide-nanoplated bismuth oxide (Bi2O3@CuS, shortly BC) composites for improved theranostic efficacy against TNBC. These systematically characterized BC nanocomposites exhibited pH-/near-infrared (NIR, 808 nm) light-responsive degradability towards dual modal therapies. Due to the band transition of Cu species, the designed BC composites displayed exceptional photothermal (PTT) conversion efficiency towards localized PTT effects. In addition to pH-/NIR-responsiveness, the internally overexpressed glutathione (GSH)-responsiveness facilitated the release of Cu2+ species for chemodynamic therapy (CDT) effects. To this end, the Bi3+ species in the core could be fully hydrated in the acidic tumor microenvironment (TME), resulting in GSH depletion and reducing CDT-induced reactive oxygen species (ROS) clearance, thereby ablating tumors. The acid-responsive degradability of CuS resulted in the intratumoral enrichment of BC, demonstrating remarkable CT imaging efficacy in vivo. Together, these pH-/NIR-/GSH-responsive biodegradable BC composites could realize the integrated PTT/CDT/CT theranostics against breast carcinoma.
研究领域:
材料科学
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纳米技术
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癌症研究
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医学成像
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治疗诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Dai Sheng,Jiang Lang,Liu Luying,Su Zhaogui,Yao Li,Yang Ping,Huang Nan
Poor wound healing in diabetics is primarily caused by persistently high levels of inflammation and recurrent bacterial infections. The catalytic therapy technique based on nanozyme medicine has emerged as a beacon of hope for patients with diabetic wounds. However, the use of a single-atom nanozyme may still have limitations, including nanozyme burst release, immunological clearance, and insufficient antibacterial activity. To address the aforementioned problems, we provide a new nano-catalytic therapeutic agent for diabetic skin ulcers that incorporates a single-atom nanozyme with high antioxidant activity into a metal-organic framework (ZIF-Cu/C-dots). First, a Cu single-atom nanozyme supported by ultra-small carbon dots (Cu/C-dots) with high antioxidant activity was created. A nanozyme-integrated metal-organic framework was then created, utilizing Cu/C-dots as ligands and Zn2+ as the core metal. Cu/C-dots have good oxidase-like activity, shielding the biological system from ROS damage and reducing the expression of TNF-α and IL-1β. Zn2+ also has good antibacterial activity (the antibacterial rate was more than 90%). This integrated technique prevents nanozyme aggregation, improves nanozyme biocompatibility, slows down the breakdown of ZIF, and allows for the regulated release of Cu/C-dots and Zn2+ as needed. Finally, in vivo studies have shown that ZIF-Cu/C-dots can effectively alleviate inflammation at the site of diabetic wounds, accelerate vascular regeneration, promote collagen deposition, and enhance tissue remodeling, serving as a novel nano-catalytic platform for the treatment of wounds that are difficult to heal.
研究领域:
纳米技术
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生物医学工程
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药学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Su Borui,Gao Dong,Xin Nini,Wu Kai,Yang Mei,Jiang Shichao,Zhang Yusheng,Ding Jie,Wu Chengheng,Sun Jing,Wei Dan,Fan Hongsong,Guo Zhenzhen
Lipid droplets (LDs) participating in various cellular activities and are increasingly being emphasized. Fluorescence imaging Provides powerful tool for dynamic tracking of LDs, however, most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield (PLQY), poor photostability and tedious washing procedures. Herein, a novel yellow-emissive carbon dot (OT-CD) has been synthesized conveniently with high PLQY up to 90%. Besides, OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2, which is much higher than most LDs probes. These characters enable OT-CD high brightness, stable and wash-free LDs probing, and feasible for in vivo imaging. Then, detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded, including ferroptosis and other diseases processes. Furthermore, fast whole imaging of zebrafish and identified LD enrichment in injured liver indicate its further feasibility for in vivo application. In contrast to the reported studies to date, this approach provides a versatile conventional synthesis system for high performance LDs targeting probes, combing the advantages of easy and high yield production, as well as robust brightness and stability for long-term imaging, facilitating investigations into organelle interactions and LD-associated diseases.
研究领域:
材料科学
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生物医学工程
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细胞生物学
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成像科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Zhou Tao,Chen Yedan,Fu Liangmin,Wang Shan,Ding Haihu,Bai Qiaosheng,Guan Jingjing,Mao Yingji
Owing to the unpredictable size of wounds and irregular edges formed by trauma, nanofibers' highly customizable and adherent in situ deposition can contribute to intervention in the healing process. However, electrospinning is limited by the constraints of conventional polymeric materials despite its potential for anti-inflammatory and antimicrobial properties. Here, inspired by the Janus structure and biochemistry of nanometal ions, we developed an in situ sprayed electrospinning method to overcome bacterial infections and immune imbalances during wound healing. The bilayer fiber scaffold has a hydrophobic outer layer composed of polycaprolactone (PCL) and a hydrophilic inner layer composed of gelatin, poly (L-lactic acid) (PLLA), and magnesium oxide nanoparticles (MgO NPs), constituting the PCL/PLLA-Gelatin-MgO (PPGM) electrospun scaffold. This electrospun scaffold blocked the colonization and growth of bacteria and remained stable on the wound for continuous anti-inflammatory properties to promote wound healing. Furthermore, PPGM electrospinning modulated collagen deposition and the inflammatory microenvironment in the full-thickness skin model, significantly accelerating vascularization and epithelialization progression. This personalized Janus electrospun scaffold has excellent potential as a new type of wound dressing for first aid and wound healthcare.
研究领域:
生物医学工程
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材料科学
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伤口愈合
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纳米技术
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）