RSC Advances
·
2019
·Vol. 9
·期 66
·38590-38596
·
IF 3.049
Rong Huang,Lu Lu Xiong,Hui Hui Chai,Jing Jing Fu,Zhisong Lu,Ling Yu
A novel colorimetric aptasensor based on a Au/Fe3O4 nanohybrid was developed to detect ochratoxin A (OTA). The aptasensor is composed of a free OTA aptamer, a Au/Fe3O4 nanohybrid coated with biotinylated complementary DNA of the OTA aptamer (biotin-cDNA-Au/Fe3O4), and free alkaline-phosphatase-labeled streptavidin (SA-ALP). The Au/Fe3O4 nanohybrid not only immobilizes biotin-cDNA but also magnetically separates SA-ALP from the sample solution. One part of the OTA aptamer sequence hybridizes with biotin-cDNA immobilized on Au/Fe3O4, and the left part of the OTA aptamer sequence covers the biotin and blocks the specific interaction between biotin and SA-ALP. OTA can interrupt the interaction of OTA aptamer binding to biotin-cDNA-Au/Fe3O4 and can inhibit the shielding effect of the OTA aptamer on biotin. The amount of SA-ALP that can be captured by biotin-cDNA-Au/Fe3O4 thus increases with increasing OTA concentration. Through a simple magnetic separation, the collected SA-ALP-linked Au/Fe3O4 can produce a yellow-colored solution in the presence of p-nitrophenyl phosphate (p-NPP). This colorimetric aptasensor can detect OTA as low as 1.15 ng mL−1 with high specificity.
研究领域:
分析化学
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纳米技术
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毒理学
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食品安全
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Huanhuan Li,Yu Wu,Xorlali Nunekpeku,Yuhan Yang,Jingsa Zhao,Wei Sheng,Yuexia Wang
The increasing presence of Aflatoxin B 1 (AFB 1 ) residues in food continues to raise significant public health concerns. Herein, we designed an upconversion-based sensor integrated into an alginate-polyacrylamide (SA-PAM) hydrogel for on-site AFB 1 detection in food. First, core-shell upconversion nanoparticles (CSUCNPs) with enhanced fluorescence were modified with complementary DNA (cDNA), forming signal probes known as CSUCNPs-cDNA. Simultaneously, NH 2 -ZIF-8@Cu 2+ with a high surface area and specific affinity for aptamer was synthesized using a one-pot method. This was then modified with an AFB 1 -specific aptamer, forming the capture probe Apt-ZIF-8@Cu 2+ . Using base pairing, CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ were hybridized into sensing probes (CSUCNPs-ZIF-8@Cu 2+ ), which were embedded into SA-PAM hydrogels as stable sensor carrier. Furthermore, the SA-PAM hydrogel showed a reduced swelling ratio of 1.68–1.80 in water and phosphate buffer solution(s) (PBS), which improves stability. In the presence of AFB 1 , the aptamer selectively binds the AFB 1 , increasing electrostatic repulsion between CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ . This disrupts Förster resonance energy transfer (FRET), resulting in a pronounced “signal-on” fluorescence. This sensor achieves AFB 1 diffusion through the SA-PAM hydrogel, FRET disruption and upconversion signal stabilization within 18 min, faster than traditional methods. Under optimized conditions, the sensor exhibited a clear linear correlation between fluorescence intensity and the logarithm of AFB 1 concentration ranging from 0.1 to 100 µg/kg ( R ² = 0.994), with a detection limit as low as 0.08 µg/kg. Besides, its performance was further validated through tests on spiked wheat, corn, peanut, and water, demonstrating high accuracy and reproducibility with relative standard deviation (RSD) below 3.63 % and recoveries of 85.88–98.04 %.
研究领域:
食品安全
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分析化学
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纳米技术
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生物传感器
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毒素检测
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产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Hao Li,Hongsen Niu,Hao Kan,Eun-Seong Kim,Nam-Young Kim,Yang Li
Traditional electronic skins (e-skins) face significant challenges in system integration and practical usability demonstrations, limiting their ability to meet the development demands of robot intellectualization under future artificial intelligence (AI) frameworks. Here, a bionic dual receptor (BDR) e-skin motivated by AI-based hardware-software coordination is proposed, which consists of electrospinning fiber triboelectric unit inspired by fast-adapting receptors and micropyramid ionic hydrogel iontronic unit inspired by slow-adapting receptors. Benefiting from the iontronic effect and micropyramid structure, the iontronic unit yields impressive features: a linear sensitivity of 172 kPa −1 (30 kPa), a fast response/recovery time of 11.2 ms. Based on this innovation, the BDR e-skin is integrated into the glove, and a dual-channel signal-motivated intelligent glove cognitive system for sign language gesture identification and robot interaction is developed, laying the foundation for subsequent usability demonstrations. Further, the BDR e-skin is deeply integrated with intelligent software algorithms and high-speed hardware circuits to construct an intelligent autonomous material cognition system. This system enables the robot finger controlled by the intelligent glove to accurately identify multidimensional properties (average accuracy: 99.3%) of smooth surface films—such as electronegativity, softness/hardness, and material species—with a single touch, showing a tactile cognition level comparable to that of humans.
研究领域:
机器人学
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电子皮肤
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人工智能
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柔性传感器
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人机交互
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触觉传感
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仿生学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Zhou Chenhui,Shi Jia,Dong Zhaoqi,Zeng Lingyou,Chen Yan,Han Ying,Li Lu,Zhang Wenyu,Zhang Qinghua,Gu Lin,Lv Fan,Luo Mingchuan,Guo Shaojun
The development of highly efficient and durable alkaline hydrogen evolution reaction (HER) catalysts is crucial for achieving high-performance practical anion exchange membrane water electrolyzer (AEMWE) at ampere-level current density. Herein, we report a design concept by employing Ga single atoms as an electronic bridge to stabilize the Ru clusters for boosting alkaline HER performance in practical AEMWE. Experimental and theoretical results collectively reveal that the bridged Ga sites trigger strong metal-support interaction for the homogeneous distribution of Ru clusters with high density, as well as optimize the Ru–H bond strength due to the electron transfer between Ru and Ga for enhanced intrinsic HER activity. Moreover, the oxophilic Ga sites near the Ru clusters tend to adsorb the hydroxyl species and accelerate the water dissociation for sufficient proton supplement in an alkaline medium. The Ru–Ga SA /N–C catalyst exhibits a low overpotential of 4 ± 1 mV (10 mA cm −2 ) and high mass activity of 9.3 ± 0.5 A mg −1 Ru at −0.05 V vs RHE. In particular, the Ru–Ga SA /N–C-based AEMWE in 1 M KOH delivers a voltage of only 1.74 V to reach an industrial current density of 1 A cm −2 , and can steadily operate at 1 A cm −2 for more than 170 h.
研究领域:
材料科学
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电化学
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催化
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能源转换与存储
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Nai-Xian Feng,Yu-Xi Feng,Qi-Feng Liang,Xin Chen,Lei Xiang,Hai-Ming Zhao,Bai-Lin Liu,Gang Cao,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming Hung Wong
Phthalate acid esters (PAEs) are environmentally ubiquitous and have aroused a worldwide concern due to their threats to environment and human health. Di-n-butyl phthalate (DBP) is one of the most frequently observed PAEs in the environment. In this study, a novel bacterium identified as Pseudomonas sp. YJB6 that isolated from PAEs-contaminated soil was determined to have strong DBP-degrading activity. A complete degradation of DBP in 200 mg/L was achieved within 3 days when YJB6 was cultivated at 31.4 °C with an initial inoculation size of 0.6 (OD600) in basic mineral salts liquid medium (MSM), pH 7.6. The degradation curves of DBP (50–2000 mg/L) fitted well the first-order kinetics model, with a half-life ( t 1/2 ) ranging from 0.86 to 1.88 d. The main degradation intermediates were identified as butyl-ethyl phthalate (BEP), mono-butyl phthalate (MBP), phthalic acid (PA) and benzoic acid (BA), indicating a new complex and complete biodegradation pathway presented by YJB6. DBP might be metabolized through de-esterification, β-oxidation, and hydrolysis, followed by entering the Krebs cycle of YJB6 as a final step. Strain YJB6 was successfully immobilized with sodium alginate (SA), polyvinyl alcohol (PVA), and SA-PVA. The immobilization significantly improved the stability and adaptability of the cells thus resulting in high volumetric DBP-degrading rates compared to that of the freely suspended cells. In addition, these immobilized cells can be reused for many cycles with well conserved in DBP-degrading activity. The ideal DBP degrading ability of the free and immobilized YJB6 cells suggests that strain YJB6, especially the SA-PVA+ YJB6 promises great potential to remove hazardous PAEs.
研究领域:
环境微生物学
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生物修复
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化学工程
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DOI
PubMed
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Qinzheng Hu,Shuzhen Cui,Kanjun Sun,Xiuting Shi,Wenxing Miao,Xiangbing Wang,Hui Peng,Guofu Ma
In recent years, the research focus of flexible supercapacitor is to improve its energy density . The recently reported improved energy storage method is to introducing suitable redox-additive in gel electrolytes to enhance their redox-activity. Herein, a novel stretchable, redox-active and double-network 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxy-containing polyacrylamide/alginate/H 2 SO 4 (PAM/SA/H 2 SO 4 /TEMPOL) hydrogel electrolyte is prepared by radical cross-linking polymerization and solvent displacement process. The PAM/SA/H 2 SO 4 /TEMPOL hydrogel possesses superior stretchability (750 %) because of the electrostatic interactions between the -NH 3+ groups of the PAM and the -COO − groups of SA . In addition, the introduction of the TEMPOL additive into PAM/SA/H 2 SO 4 /TEMPOL hydrogel electrolyte of flexible EDLC can gain a high specific capacitance (386 F g −1 ) and high energy density of 24.5 Wh kg −1 , which is due to dual redox reactions both positive/negative electrode. This study provides a new strategy for designing a multifunctional redox hydrogel electrolyte.
研究领域:
材料科学
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电化学
·
能源存储
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Jiasheng Wang,Luyu Sun,Yaxuan Zuo,Ni Hui
Electrochemical biosensors offer a cost-effective and noninvasive approach for continuously monitoring cortisol in complex bodily fluids like blood, sweat, or saliva. However, their high sensitivity makes them vulnerable to biological contamination when used with biological samples. In this study, an electrochemical sensor was developed using an antifouling molecularly-imprinted hydrogel that combines the antifouling properties of a conducting polymer hydrogel with the recognition capabilities of a molecularly-imprinted polymer (MIP). The conductive hydrogel, composed of poly(3,4-ethylenedioxythiophene)/sodium alginate (PEDOT/SA), was synthesized through an electrochemical gelation technique. This hydrogel displayed a well-connected structure at the macroscopic level, leading to an increased effective surface area for the resulting electrochemical sensor. The MIP electrochemical sensor effectively minimized non-specific adsorption and demonstrated high sensitivity and selectivity in detecting trace amounts of cortisol in complex physiological fluids. Detection of the target analytes was accomplished using electrochemical impedance spectroscopy, with a linear range spanning from 10 –12 to 10 –8 M and a detection limit of 0.314 pM, as well as chronocoulometry, with a linear range from 10 –12 to 10 –7 M and a detection limit of 0.131 pM. The sensor showed commendable performance in accurately detecting cortisol in diverse bodily fluids such as saliva, sweat, and serum. By combining the MIP with a conductive hydrogel that resists biofouling, the electrochemical sensor exhibited high specificity, sensitivity, and exceptional resistance to interference when used in practical biological applications.
研究领域:
生物传感技术
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材料科学
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医学检测
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DOI
高影响因子
Acta Biomaterialia
·
2015
·
IF 6.025
Tiantian Yin,Licong Yang,Yanan Liu,Xianbo Zhou,Jing Sun,Jie Liu
The blood–brain barrier (BBB) is a formidable gatekeeper toward exogenous substances, playing an important role in brain homeostasis and maintaining a healthy microenvironment for complex neuronal activities . However, it also greatly hinders drug permeability into the brain and limits the management of brain diseases. The development of new drugs that show improved transport across the BBB represents a promising strategy for Alzheimer’s disease (AD) intervention. Whereas, previous study of receptor-mediated endogenous BBB transport systems has focused on a strategy of using transferrin to facilitate brain drug delivery system, a system that still suffers from limitations including synthesis procedure, stability and immunological response. In the present study, we synthetised sialic acid (SA)-modified selenium (Se) nanoparticles conjugated with an alternative peptide-B6 peptide (B6-SA-SeNPs, a synthetic selenoprotein analogue), which shows high permeability across the BBB and has the potential to serve as a novel nanomedicine for disease modification in AD. Laser-scanning confocal microscopy , flow cytometry analysis and inductively coupled plasma-atomic emission spectroscopy ICP-AES revealed high cellular uptake of B6-SA-SeNPs by cerebral endothelial cells (bEnd.3). The transport efficiency of B6-SA-SeNPs was evaluated in a Transwell experiment based on in vitro BBB model. It provided direct evidence for B6-SA-SeNPs crossing the BBB and being absorbed by PC12 cells. Moreover, inhibitory effects of B6-SA-SeNPs on amyloid-β peptide (Aβ) fibrillation could be demonstrated in PC12 cells and bEnd3 cells. B6-SA-SeNPs could not only effectively inhibit Aβ aggregation but could disaggregate preformed Aβ fibrils into non-toxic amorphous oligomers . These results suggested that B6-SA-SeNPs may provide a promising platform, particularly for the application of nanoparticles in the treatment of brain diseases. Statement of Significance Alzheimer’s disease (AD) is the world’s most common form of dementia characterized by intracellular neurofibrillary tangles in the brain. Over the past decades, the blood–brain barrier (BBB) limits access of therapeutic or diagnostic agents into the brain, which greatly hinders the development of new drugs for treating AD. In this work, we evaluated the efficiency of B6-SA-SeNPs across BBB and investigated the interactions between B6-SA-SeNPs and amyloid-β peptide (Aβ). We confirm that B6-SA-SeNPs could provide a promising platform because of its high brain delivery efficiency, anti-amyloid properties and anti-oxidant properties, which may serve as a novel nanomedicine for the application in the treatment of brain diseases.
研究领域:
神经科学
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药学
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Miaolu He,Yushuang Li,Leihao Feng,Jiaqi Wang,Ze Liu,Lei Wang,Jin Wang,Rui Miao,Xudong Wang
The traditional thin-film composite (TFC) forward osmosis (FO) membrane has problems such as poor water permeability and low lithium concentration selectivity because the separation layer is too thick and has many defect pores. In this study, a thin and dense separation layer was obtained by constructing a sodium alginate (SA)/UiO-66-NH 2 interlayer to control the attachment amount and diffusion rate of m-phenylenediamine (MPD), which enhanced the performance of the membrane. This paper studies the effect and mechanism of the interlayer on the structure and separation performance of the FO membrane, and explores the lithium ion concentration performance of the interlayer membrane. According to the results, based on the strong interaction between the interlayer and MPD, the equilibrium concentration of MPD available for interfacial polymerization was increased and the diffusion rate of MPD was slowed, resulting in the generation of a thin and dense separation layer. Since the interlayer restricted the escape of bubbles formed in the interfacial polymerization, the separation layer showed a leaf-like structure with increased filtration area and water storage space. Due to the reduced thickness of the separation layer and its leaf-like structures, the water flux increased significantly, and the water flux of the interlayer membrane was 47.9% greater than that of the original membrane. Drawing upon the heightened density of the separation layer and secondary separation of the interlayer, the reverse solute flux was lowered by 37.4%. The interlayer membrane exhibited efficient Li + rejection (R>93%) and the concentration ratio of Li + was close to its theoretical concentration ratio at 1.46 ± 0.03.
研究领域:
材料科学
·
环境工程
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Derrick Boateng,Xukai Li,Weiyao Wu,Anqi Yang,Anadil Gul,Yan Kang,Lin Yang,Jifang Liu,Hongbo Zeng,Hao Zhang,Linbo Han
Accurate air-writing recognition is pivotal for advancing state-of-the-art text recognizers, encryption tools, and biometric technologies. However, most existing air-writing recognition systems rely on image-based sensors to track hand and finger motion trajectories. Additionally, users’ writing is often guided by delimiters and imaginary axes which restrict natural writing movements. Consequently, recognition accuracy falls short of optimal levels, hindering performance and usability for practical applications. Herein, we have developed an approach utilizing a one-dimensional convolutional neural network (1D-CNN) algorithm coupled with an ionic conductive flexible strain sensor based on a sodium chloride/sodium alginate/polyacrylamide (NaCl/SA/PAM) dual-network hydrogel for intelligent and accurate air-writing recognition. Taking advantage of the excellent characteristics of the hydrogel sensor, such as high stretchability, good tensile strength, high conductivity, strong adhesion, and high strain sensitivity, alongside the enhanced analytical ability of the 1D-CNN machine learning (ML) algorithm, we achieved a recognition accuracy of ∼96.3% for in-air handwritten characters of the English alphabets. Furthermore, comparative analysis against state-of-the-art methods, such as the widely used residual neural network (ResNet) algorithm, demonstrates the competitive performance of our integrated air-writing recognition system. The developed air-writing recognition system shows significant potential in advancing innovative systems for air-writing recognition and paving the way for exciting developments in human-machine interface (HMI) applications.
研究领域:
电气工程
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机器学习
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材料科学
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人机交互
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
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2024
·
IF 6.8
Xin Xiang,Chuning Dong,Lianbo Zhou,Jun Liu,Zachary M. Rabinowitz,Yuzhao Zhang,Honghui Guo,Feng He,Xingdou Chen,Yunhua Wang,Lina Cui,Xiaowei Ma
Real-time detection of cellular senescence remains a clinical challenge. Here, we aimed to develop a positron emission tomography (PET) imaging probe targeting senescence-associated β-galactosidase (SA-β-Gal), the most widely used biomarker of cellular senescence, and investigate its performance for real-time in vivo quantitative detection of cellular senescence. A stable PET imaging agent [68Ga]Ga-BGal was obtained with a high labeling yield (90.0 ± 4.3%) and a radiochemical purity (>95%). [68Ga]Ga-BGal displayed high sensitivity and specificity for β-Gal both in vitro and in vivo. The reaction and uptake of the probe correlated with the β-Gal concentration and reaction time. In PET imaging, high β-Gal-expressing CT26.CL25 tumors and doxorubicin-treated HeLa tumors showed high signals from [68Ga]Ga-BGal, while a low signal was observed in CT26.WT and untreated HeLa tumors. In summary, we showcased successful PET imaging of senescence in preclinical models using probe [68Ga]Ga-BGal. This finding holds the potential for translating senescence imaging into clinical applications.
研究领域:
医学影像学
·
分子生物学
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肿瘤学
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放射学
·
药学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 46
·期 4
·1569-1576
·
IF 3.591
Zhuoyao Ni,Jiajie Hu,Hui Zhu,Yazhuo Shang,Daijie Chen,Yinghong Chen,Honglai Liu
Antibiotic resistance caused by long-term abuse of antibiotics has inevitably become a very serious problem and developing novel strategies to enhance the efficacy of treatments is exigent. Herein, a dual-antibacterial platform using sodium alginate (SA) and carboxymethyl chitosan (CMCS) as the framework was designed for NIR controlled pharmacotherapy and photothermal therapy of drug-resistant bacteria. The SA/CMCS solution can quickly transform into a hydrogel in response to Ca2+ and Mg2+ in biological bodies. By mixing indocyanine green (ICG) and thermosensitive drug-loaded liposomes into the solution, this designed platform can not only achieve near-infrared (NIR) controlled drug release properties but also possess dual-antibacterial capacity by combining pharmacotherapy and photothermal therapy (PTT). Local temperature rise induced by ICG will break the stability of thermosensitive liposomes, causing the release of the encapsulated drug. The in vitro antibacterial test and in vitro cytotoxicity experiment demonstrate the excellent antibacterial capacity and satisfactory biocompatibility of the prepared platform. The proposed NIR controlled dual-antibacterial platform can not only avoid bacterial resistance but also kill drug-resistant bacteria efficiently, which will provide a novel concept for the development of an intelligent antibacterial platform.
研究领域:
药学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）