TALANTA
·
2021
·
IF 6.057
Yaliang Huang,Ting Sun,Lin Liu,Ning Xia,Yuehua Zhao,Xinyao Yi
The hybridization and enzymolysis reactions for nucleic acid detection were carried out on the chip surface in the traditional surface plasmon resonance (SPR) biosensors. Herein, we proposed an innovative method for microRNA (miRNA) detection in which the hybridization-enzymolysis recycling reactions were performed in solution. Duplex-specific nuclease (DSN) and streptavidin-modified gold nanoparticles (SA-AuNPs) were employed for enhancing the assay sensitivity. In the absence of miRNA, the biotinylated DNA probe (bio-DNA-bio, biotin tags at both the 3′ and 5′ termini of DNA) was attached to the SA-modified chip through the SA-biotin binding, allowing the capture of SA-AuNPs with the same interaction. As a result, a larger SPR signal was attained. However, in the presence of miRNA, bio-DNA-bio hybridized with miRNA was digested by DSN. In this process, the miRNA strand remained intact and participated in the next hybridization-enzymolysis recycling process. Thus, one miRNA could promote the hydrolysis of many bio-DNA-bio probes and allow the generation of numerous bio-DNA fragments. Meanwhile, the produced bio-DNA competed with the undigested bio-DNA-bio to bind SA on the chip surface. The digestion of bio-DNA-bio and the competitive binding between bio-DNA-bio and bio-DNA led to the attachment of fewer SA-AuNPs and then smaller SPR signals. The change in SPR signal at the concentration as low as 1 fM miRNA has been readily determined. The strategy possessed the advantageous properties of simple operation, fast response, high sensitivity and excellent specificity, serving as a viable means for the fabrication of novel sensing platforms.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Li Xuegang,Zhao Xingyu,Zhang Jing,Zhou Xue,Zhang Yanan,Zhao Yong,Warren-Smith Stephen C.,Nguyen Linh Viet
Highly sensitive RNA detection at ultralow concentrations
remains
challenging for conventional surface plasmon resonance (SPR) biosensors
because low-molecularI biomolecules induce only weak effective refractive-index
perturbations within the limited evanescent-field penetration depth.
Here, we report a dual-scale gold nanoparticle (AuNP)–enhanced
fiber-optic SPR biosensor that overcomes this intrinsic limitation
through a mechanism-guided sandwich-type architecture. The sensor
employs a multimode fiber–single-mode fiber–multimode
fiber (MMF–SMF–MMF) configuration to enhance evanescent-field
excitation at the metal–dielectric interface. Plasmonic amplification
is achieved by decoupling probe immobilization and signal enhancement
into two AuNP populations with distinct roles: large AuNPs functionalized
with probe DNA form a stable, high-density recognition layer on the
gold-coated fiber surface, while small AuNPs conjugated with target
RNA act as mobile amplification units. Upon specific DNA–RNA
hybridization, a hierarchical plasmonic sandwich structure is formed
within the effective sensing volume, enabling synergistic enhancement
through near-field electromagnetic coupling and mass-loading effects.
Finite-element simulations and experiments consistently demonstrate
substantially stronger localized electromagnetic fields and higher
refractive-index sensitivity compared with bare gold films and monolayer
AuNP-modified SPR sensors. Importantly, the assay does not rely on
nucleic-acid amplification reactions or fluorescent labeling. The
sensor exhibits a linear response to RNA concentrations from 0 to
700 fM, achieving a sensitivity of 6.5 pm/fM and a detection limit
of 97 fM, corresponding to a 7.5-fold signal enhancement over conventional
single-layer AuNP-based SPR sensors. This work demonstrates that a
dual-scale AuNP-assisted sandwich architecture can effectively amplify
weak SPR responses from thiol-modified model RNA targets without requiring
nucleic-acid amplification reactions. Further optimization toward
direct detection of unmodified RNA in real biological samples will
be pursued in future work.
✓ 已验证
产品关联
DOI
高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2026
·
IF 7
Si-qi Zhang,Yong Zhao,Yun Peng,Lu Cai,Sen Qin,Jia-shuo Zhang,Cong-hai Ge
A dual-channel fiber optic surface plasmon resonance (SPR) sensor featuring a micro-conical design is developed to enable highly sensitive and concurrent sensing of relative humidity (RH) and temperature. The humidity-sensing channel is constructed relying on the micro-conical fiber structure, deposited with a gold layer as well as a nanoscale-thickness polyvinyl alcohol (PVA) film, which can undergo rapid volume changes in response to humidity. The conical fiber tip enhances the evanescent field, thereby increasing the interaction between surface plasmon polaritons (SPPs) and the nanoscale PVA film, and realizing high-performance RH sensing. Cascaded with the micro-conical structure is the temperature-sensing channel, which is built using a twin-core dual-hole fiber (TCDHF) combined with polydimethylsiloxane (PDMS), aiming to achieve simultaneous temperature measurement. Simulation and preliminary experimental results showed that within a certain range, a thicker nanoscale PVA film leads to a longer resonant wavelength and a higher response to humidity. Further dual-parameter experimental results indicated that when three layers of PVA are integrated on the surface, the developed dual-channel SPR probe exhibits outstanding RH and temperature sensing performance. It can provide an RH sensitivity up to 2.80 nm/%RH and –2.66 nm/°C for temperature. Meanwhile, dynamic response tests verify that the sensor has reliable cyclic consistency, achieving fast response times of 7 s and 12.67 s for the RH increasing and decreasing processes, respectively. Additionally, with its reusability and compact design, the sensor is expected to provide a promising solution for the rapid and reliable humidity and temperature monitoring in confined spaces.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Jianguo Hou,Binyun Xia,Kunpeng Niu,Jiale Wang,Jinhua Li,Ning Wang
Background Cobalt, an essential trace element, is vital for maintaining human nervous system function, aiding in DNA synthesis, and contributing to red blood cell production. It is helpful for disease diagnosis and treatment plan evaluation by precisely monitoring its concentration changes in the human body. Despite extensive efforts made, due to its ultra-low concentration, the current limit of detection (LOD) as reported is still inadequate and cannot be satisfied with the precise clinical applications. Therefore, it is crucial to develop novel label-free sensors with high sensitivity and excellent selectivity for detecting trace amounts of Co 2+ . Results Here, an ultrasensitive optical fiber SPR sensor was designed and fabricated for label-free detection of Co 2+ with ultra-low concentration. It is achieved by modifying the carboxyl-functionalized CQDs on the AuNPs/Au film-coated hetero-core fiber, which can specifically capture the Co 2+ , leading to changes in the fiber's surface refractive index (RI) and subsequent SPR wavelength shifts in the transmission spectrum. Both the Au film and AuNPs on the fiber are modified with CQDs, leveraging their large surface area to enhance the number of active sites and probes. The sensor exhibits an ultra-high sensitivity of approximately 6.67 × 10 19 nm/M, and the LOD is obtained as low as 5.36 × 10 −20 M which is several orders of magnitude lower compared to other conventional methods. It is also experimentally demonstrated that the sensor possesses excellent specificity, stability, and repeatability, which may be adapted for detecting real clinical samples. Significance The CQDs-functionalized optical fiber SPR sensor exhibits substantial potential for precisely detecting Co 2+ of trace amounts, which is especially vital for scarce clinical samples. Additionally, the sensing platform with sample sensor fabrication and measurement configuration introduces a novel, highly sensitive approach to biochemical analysis, particularly adapting for applications involving the detection of trace targets, which could also be employed to detect various biochemical targets by facile modification of CQDs with specific groups or biomolecules.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 10
·13305-13322
·
IF 2.478
Gu Yufen,Guo Bobo
Here, the novel Ag 2 MoO 4 /Bi 2 WO 6 (AMO/BWO) heterojunction composite photocatalysts were synthesized based on the combination (co-precipitation method + hydrothermal method) XRD, XPS, SEM, TEM, UV–Vis DR spectroscopy, PL spectroscopy, FTIR spectroscopy, as well as EIS analyses were employed to characterize the photocatalysts, and photocurrent response was utilized to confirme the formation of Z-type heterojunction. The generation and transfer of electron/holes in the AMO/BWO heterojunctions excited by simulated sunlight were analyzed. Under simulated sunlight, the photocatalytic performance of AMO/BWO composites were studied by degradation of organic pollutants. The experimental results show that the 30 wt% AMO@BWO is the best photocatalytic composite material among all samples and the degradation rate reached 98.6%. Due to the prolonging of lifetime of the photoexcited electrons in the CB of AMO and holes in the VB of BWO, these carriers are more effective to participate in photodegradation reactions. An electron/hole transfer mechanism of the Z-scheme heterojunctions is proposed to illustrate the enhanced photodegradation performance of the AMO/BWO composite samples.
研究领域:
材料科学
·
环境科学
·
光催化技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xueqi Duan,Xing Li,Qingya Yang
Due to the serious threat of excessive copper and magnesium ions to human health, it is crucial to detect copper and magnesium ions in aquatic environments. In this study, we propose a colorimetric strategy – gold nanoparticles (AuNPs) modified by 5-(3-aminophenyl)-1H-tetrazole (TA@AuNPs) for the rapid and portable identification of copper and magnesium ions in water. As the concentration of the target metal ions increases, the colorimetric principle (TA@AuNPs) causes the aggregation of the AuNPs system, shifts of the surface plasma resonance (SPR) absorption peak, and a change of system colors (wine-red to gray-blue). The advantages of this work: 1) the detection process is simple without complex pretreatment; 2) the concentration of the target ions could be quickly detected within 3 min by Standard Color Cards; 3) it can selectively detect two kinds of metal ions (Cu 2+ and Mg 2+ ) with high specificity in the presence of other interfering ions; 4) It eliminates the interference of calcium ions in the detection of magnesium ions; 5) It has excellent linearity and low detection limit (for Cu 2+ : R 2 = 0.94, LOD = 30.0 μM by the naked eye and 0.229 μM through ultraviolet–visible spectroscopy; for Mg 2+ : R 2 = 0.96, LOD = 300 μM by the naked eye and 0.653 μM through ultraviolet–visible spectroscopy). Furthermore, the colorimetric mechanism of the present work has been comprehensively demonstrated through DLS, TEM, zeta potential analysis, FT-IR, UV–vis and Gaussian calculations.
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 48
·期 4
·1502-1509
·
IF 3.3
Yansheng Liu,Junpeng Deng,Xiaobo Jia,Jin Zhou,Hongli Li,Xiaohong Wang,Yating Chen,Zhenle Qin,Zhichen Jin,Guofu Wang
Quick and highly sensitive detection of the SARS-CoV-2 virus can efficiently reduce the possibility of viral spreading. In this study, a differential-phase surface plasmon resonance (SPR) biosensor is constructed to directly detect the SARS-CoV-2 virus. The SARS-CoV-2 spike protein antibodies are applied as bioprobes and functionalized on Au film, forming a sensing surface. A numerical analysis of the phase shifts of the SPR interferogram, termed the index of phase shifts (IoPS), is presented, which does not depend on a reference beam or an s-component beam. The IoPS can sensitively reflect small changes in the refractive index (as low as 3.75 × 10−10 RIU) when NaCl solution is used as an analyte. The limit of detection (LOD) of the proposed SPR biosensor in detecting the SARS-CoV-2 S protein is 1 ag mL−1. In comparison with a SARS-CoV-2 negative control (NC) sample collected from healthy people, which has an IoPS of ΔϕNC = 39°, the SPR biosensor shows a very strong response to the SARS-CoV-2 virus collected from infected people with a phase shift of ΔϕIoPS = 566°. To further improve the detection sensitivity, a sandwich structure is designed by applying SARS-CoV-2 spike protein antibody functionalized gold nanoparticles (anti-S@AuNPs). This reduces the LOD for the detection of the SARS-CoV-2 S protein to 0.1 ag mL−1.
研究领域:
生物传感器技术
·
病毒学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Qichao Hu,Yuan Chen,Wenwen Zhang,Yubing Li,Xinyu Deng,Lisheng Chen,Zongyuan Zhou,Xiao Ma,Jinhao Zeng,Yanling Zhao
Background Acute gastric injury, a common and recurring global digestive disorder, significantly impairs patient quality of life and overall health. Dehydroevodiamine (DHE), a bioactive natural product derived from Tetradium ruticarpum ( A. Juss. ) Hartley, shows potential therapeutic effects on acute gastric injury. This study investigates the underlying mechanisms of DHE's alleviating effects on acute gastric injury. Methods The gastric mucosal protective effect of DHE was confirmed through in vivo and in vitro acute gastric injury models. Biotin pulldown MS and molecular dynamics simulations identified DHE's target. CETSA and SPR assays validated DHE's affinity for IKKβ. Protein site mutation validation and MST pinpointed the direct binding sites of DHE on IKKβ. Additionally, the potential mechanism by which DHE ameliorates acute gastric injury was elucidated using WB, IHC, and IF methods, and further confirmed by rescue experiments. Results DHE effectively ameliorated IDO-induced gastric injury in GES-1 cells and rat gastric mucosa, both in vitro and in vivo . Biotin pulldown MS identified IKKβ as the target of DHE in alleviating gastric injury. CETSA and SPR assays confirmed DHE's direct binding to IKKβ. Molecular dynamics simulations, protein mutation experiments, and MST results pinpointed GLU-149, GLU-49, and ASP-103 in the ATP-binding pocket as the binding sites of DHE on IKKβ. Notably, DHE was found to competitively bind to IKKβ with ATP. Mechanistically, DHE attenuated IDO-induced gastric injury by inhibiting the IKKβ-p65/NLRP3 signaling pathway. Importantly, exogenous activation of IKKβ reversed the therapeutic effect of DHE, indicating that DHE's efficacy depends on IKKβ. Conclusion DHE attenuated IDO-induced gastric injury by inhibiting the IKKβ-p65/NLRP3 signaling pathway. Notably, DHE is a novel ATP-competitive IKKβ inhibitor that prevents phosphorylation by targeting GLU-149, GLU-49, and ASP-103 in the ATP-binding pocket. This study reveals new targets of action for DHE, providing a new molecular basis for using DHE in treating inflammation-related diseases.
研究领域:
药理学
·
胃肠病学
·
分子生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE CERAMIC SOCIETY OF JAPAN
·
2021
·Vol. 129
·期 12
·731-738
·
IF 1.224
Ruirui LIU,Qi ZHAO,Zhijiang JI
Ternary Au/Bi2WO6/BiOBr composites were skillfully prepared via a two-step synthetic route. The two-component Bi2WO6/BiOBr heterojunction was firstly fabricated by hydrothermal method, and Au nanoparticles (NPs) were further introduced and uniformly anchored on the surface of Bi2WO6/BiOBr, where the loaded Au NPs could be beneficial for improving visible-light absorption and adjusting photoinduced charge carriers in the heterostructure through surface plasmon resonance (SPR). In comparison with pristine Bi2WO6 and two-component Bi2WO6/BiOBr, the ternary Au/Bi2WO6/BiOBr composite exhibits a better photocatalytic activity for removal of rhodamine B (RhB) under visible light irradiation. The degradation efficiency of GTB-3 composite 1.9 times higher than that of the pure Bi2WO6 and 1.24 times than that of Bi2WO6/BiOBr. Trapping tests with different scavengers revealed that not hydroxyl radicals but superoxide radicals and photogenerated holes were highly responsible for the degradation process. The enhanced photocatalytic activity was ascribed to the synergetic effect of heterojunction effect (efficient separation of charge carriers), porous structure of Bi2WO6 (strong adsorption), SPR of Au.
研究领域:
材料科学
·
化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 10
·3490-3500
·
IF 3.348
Wu Zhang,Weikang Xia,Yasheng Shi,Changlin Zhou,Ran Chen,Lei Wang,Jinqing Qu
Flame-retarded epoxy thermosets are highly desirable for electronics-related basic materials in the new-generation information applications. Herein, a DOPO-anchored benzothiadiazole flame retardant, namely DOPO-BTD , is designed and synthesized for P/N/S synergistic flame-retarded epoxy thermosets. DOPO-BTD possesses good thermal stability and excellent compatibility with the epoxy matrix. As reflected by UL 94 test, the DOPO-BTD coated EP passes the V0 rating and achieves a higher LOI value of 34.6% at ultralow P content of 0.39 wt%. The investigation of the DOPO-BTD -triggered P/N/S synergistic flame-retarded effect by thermal images further validated the flame retarded efficacy and dramatic inhibition of the flame. Meanwhile, the incorporation of 4.8 wt% DOPO-BTD into the epoxy matrix, the PHRR, THR, SPR, TSP, and COP are significantly decreased by 37.97%, 27.44%, 25.49%, 19.83%, and 31.70%, respectively. Additionally, the fire growth index (FGI) is sharply dropped 26.06%. Moreover, the TGIR and TGMS results convince the flame-retarded mechanism of DOPO-BTD through the combination of the gas-phase and condensed-phase pathways. The P/N/S synergistic DOPO-BTD can serve as a promising flame retardant for efficiently fabricating the flame-retarded epoxy thermosets, and this study sheds light on the development of multiple elements synergistic flame-retardants for functional polymers with great application potential.
研究领域:
材料科学
·
化学工程
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Hongxin Tan,Yuanshuai Song,Yi Zhao,Ziteng Shen,Yuxia Zhao,Xiuqin Zhang,Hongyan Zhang
Biothiols, such as glutathione (GSH) play vital roles in various physiological processes in the human body, and their abnormal expression will cause serious implications for health. Therefore, the ultrasensitive detection of GSH is of great importance. In this study, we present a novel method for GSH detection using a fluorescence microscopy–surface plasmon resonance (FM-SPR) system, combined with a surface plasmon resonance (SPR) sensor chip functionalized with carbon dots and gold nanoparticles. The proposed method exhibited high sensitivity and specificity for GSH detection in a phosphate-buffered saline (PBS) and 2 % fetal bovine serum (FBS) environment, as low as 0.1 ng/mL GSH can be detected during the range of 0.1–100 ng/mL, with a correlation coefficient (RWu et al. (2004) [ 2 ]) of 0.988 and 0.986 respectively. Moreover, the number of green fluorescence points observed on the FM-SPR chip surface increased with the GSH concentration, correlating well with the corresponding SPR signal changes. To evaluate the performance in more complex solutions, we further validated the method in 2 % FBS. The results demonstrated successful detection of GSH in FBS, achieving a detection limit as low as 0.1 ng/mL and a linear range of 0.1–100 ng/mL with a linearity of 0.986. Notably, in both PBS and FBS, the method maintained excellent selectivity for GSH in the presence of ten potentially interfering amino acids. These findings highlight the potential of this FM-SPR-based biosensing platform for practical applications in the detection of disease-related biomarkers such as GSH.
研究领域:
生物传感
·
分析化学
·
纳米技术
·
生物医学工程
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Jinjuan Gao,Wen Yang,Runcheng Liu,Jingwen Feng,Yaru Li,Mingshun Jiang,Shouzhen Jiang
The optical fiber sensor, which exploits surface plasmon resonance (SPR), finds extensive application in biological detection owing to its miniaturized and real-time measurement capabilities. Nevertheless, SPR sensors alone exhibit limited sensitivity when detecting small biological molecules. This paper presents a novel gold nanoparticle (AuNPs)/two-dimensional metal–organic framework (2D MOF Cu-TCPP)/Au/D-shaped fiber SPR sensor for dopamine detection. The composite structure of AuNPs/2D MOF/Au was designed to facilitate the coupling of SPR and local surface plasmon resonance (LSPR), resulting in a substantial enhancement of the intensity of the surface electric field. A 104.61 % increase in sensitivity and a 73.87 % increase in figure of merit (FOM) were achieved by optimizing the optimal deposition concentration of AuNPs/2D MOF . The finite element method was utilized in COMSOL software to demonstrate that the coupling of SPR and LSPR can significantly enhance the sensitivity value (10,000 nm/RIU). Additionally, the sensitization mechanism of the sensor is elucidated by the energy band theory. Moreover, probe DNA was utilized as a linker to capture dopamine molecules, achieving a low limit of detection (LOD) of 1.07 ± 0.07*10 −14 M. These results demonstrated that the proposed sensor enables accurate and highly sensitive detection of dopamine molecules.
研究领域:
生物医学工程
·
纳米技术
·
光学传感
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）