ANALYST
·
2022
·Vol. 147
·期 5
·956-964
·
IF 5.227
Yiyin Zhang,Xiang Gao,Yingwang Ye,Yizhong Shen
Rapid and accurate monitoring of food freshness to provide consumers with high-quality meat continues to be of tremendous importance to the food industry. In this report, an efficient Fe-doped polydopamine (Fe-PDA) nanozyme with peroxidase-mimicking activity was synthesized by a high-temperature hydrothermal method, and was applied to a spectrophotometric sensing system, which successfully reports the concentration of hypoxanthine (Hx) related to meat freshness. The Fe-PDA nanozyme showed excellent peroxidase simulation activity, which was primarily verified by steady-state kinetics experiments. In the presence of xanthine oxidase (XOD), Hx can react quantitatively with dissolved O2 to generate H2O2, which can be further catalyzed and produce hydroxyl radicals (•OH) under acidic conditions via the Fe-PDA nanozyme and oxidize colorless TMB to blue oxTMB with absorbance at 653 nm. The absorbance at 653 nm expressed a clear linear relationship with hypoxanthine concentration in the range of 5.13–200 μM, and the detection limit was 1.54 μM. This method was further assessed by measuring the recovery of Hx added to meat samples, which showed promising accuracy. Overall, the developed Fe-PDA nanozyme with excellent peroxidase-mimicking activity is cost-effective, high-performance and easy to produce, offering an efficient and low-cost sensing system based on spectrophotometry for meat freshness determination as an alternative to conventional methods.
研究领域:
食品科学
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纳米技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Zheng Shen,Deyan Xu,Guangxian Wang,Lingjun Geng,Rui Xu,Guanjie Wang,Yemin Guo,Xia Sun
For the visual detection of four organophosphorus pesticides (OPs), a colorimetric aptasensor was developed based on aptamer-mediated bimetallic metal-organic frameworks (MOFs) nano-polymers. Fe-Co magnetic nanoparticles (MNPs) and Fe-N-C nanozymes were prepared based on pyrolytic reaction, and were labeled with broad spectrum aptamers and complementary chains of organophosphorus pesticides respectively. The hybridization of aptamers and complementary chains led to the formation of nano-polymers. In the presence of target pesticides, they competed with complementary chains for aptamers on Fe-Co MNPs, resulting in a large number of Fe-N-C nanozymes signal labels being released into the supernatant. Fe-N-C nanozymes showed similar activity to peroxidase and catalyzed the 3,3′,5,5′-tetramethylbenzidine-hydrogen peroxide (TMB-H 2 O 2 ) color system to turn the solution blue-green under mild conditions. The magnetic probes had good selectivity and sensitivity, and were easily separated by magnetic absorption . The sensor functioned well under optimal conditions, demonstrating good stability and specificity for four pesticides: phorate, profenofos , isocarbophos and omethoate, and the detection limits of four pesticides were as low as 0.16 ng/mL, 0.16 ng/mL, 0.03 ng/mL and 1.6 ng/mL respectively, and the recovery rate of OPs residue in vegetable samples was satisfactory. The work described here provided a simple, rapid and sensitive way to construct a biosensor .
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Kun Ge,Yihong Huang,Hanqiang Zhang
In this paper, we report a novel surface-enhanced Raman spectroscopy (SERS) substrate based on hierarchical β-Bi 2 O 3 /Au 2 Ag 2 microspheres for rapid, sensitive and selective detection of environment pollutants including o -dianisidine ( o -diASD) and Hg 2+ in environmental samples. The sheet-like β-Bi 2 O 3 not only provides large specific surface areas for adsorption of molecules and AuAg, but also emerges as semiconductor matrix with chemical enhancement combined with AuAg with electromagnetic enhancement, making promising SERS activity. Particularly, the β-Bi 2 O 3 /Au 2 Ag 2 shows high SERS performance for 4-mercaptobenzoic acid and TMB with minimum detectable concentration of 0.1 μg/L with enhancement factor of 3.1 × 10 7 and 6.3 × 10 7 , respectively. The density functional theory simulations were further adopted to explain the high SERS activity and selectivity for o -diASD and TMB. Finally, the β-Bi 2 O 3 /Au 2 Ag 2 was applied to direct detection of o -diASD, and indirect detection of Hg 2+ by TMB marking in environmental samples. The linearity range of 0.5–200.0 and 0.2–500.0 μg/L with limit of detection of 0.2 and 0.07 μg/L for o -diASD and Hg 2+ ions can be achieved, respectively. This method provides a novel strategy in designing and fabricating SERS substrates with high performance for rapid, sensitive and accurate analysis of environmental pollutants.
研究领域:
环境科学
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分析化学
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材料科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 18
·2948-2958
·
IF 2.7
Bin Liu,Yu Yin,Qianwen Li,Wanwan Li,Fubing Xiao,Jinquan Liu,Yan Tan,Shengyuan Yang
Herein, a novel type of phosphorus and iron-doped carbon dot (P,Fe-CD) with outstanding peroxidase activity and excellent fluorescence performance was hydrothermally synthesized to colorimetrically and fluorimetrically detect tannic acid (TA). In the presence of 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2, the P,Fe-CDs could oxidize colorless TMB to a blue oxidation product (oxTMB) resulting in an increased value of absorbance. Simultaneously, the fluorescence intensity of P,Fe-CDs at 430 nm could be quenched owing to the fluorescence resonance energy transfer (FRET) between P,Fe-CDs and the generated oxTMB. Meanwhile, after adding the TA to the system containing TMB, H2O2 and P,Fe-CDs, the value of absorbance could be decreased and the fluorescence could be recovered because of the reduction reaction between TA and oxTMB. Therefore, fluorescence intensity and value of absorbance could be applied to quantitatively detect TA with good linearities between the concentration of TA and the fluorescence intensity/value of absorbance (0.997 and 0.997 for the colorimetric signal and fluorimetric one, respectively) and low limits of detection (0.093 μmol L−1 and 0.053 μmol L−1 for the colorimetry and the fluorimetry, respectively), which was successfully applied to the detection of TA in red wines. Moreover, we applied a smartphone-assisted method to the point-of-care detection of TA with accurate results, providing a new technique for TA detection and food quality monitoring.
研究领域:
分析化学
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 412
·期 2
·499-506
·
IF 3.286
He Shao-Bin,Chen Feng-Qing,Xiu Ling-Fang,Peng Hua-Ping,Deng Hao-Hua,Liu Ai-Lin,Chen Wei,Hong Guo-Lin
Although a massive research has been devoted on the exploration of noble metal–based nanozyme, less progress has been made in the investigation of palladium (Pd) nanozyme and the interaction between ions and Pd nanozyme. In this study, a new type of Pd nanozyme was prepared by a facile one-pot approach by using carboxylated chitosan as the stabilizer. Owing to the synergistic effect of carboxylated chitosan stabilized Pd nanoparticles (CC-PdNPs) can effectively catalyze the H 2 O 2 -mediated oxidation of 3,3′,5,5′-tetramethylbenzidine sulfate (TMB) accompanied by a blue color change (oxidized TMB), indicating the peroxidase-like activity of CC-PdNPs. Furthermore, the Michaelis-Menten constants and catalytic stability of CC-PdNPs render them suitable for environmental analysis and bio-detection. Here, we found that while introducing the iodine ions (I − ) into the reaction medium, the peroxidase-like activity of CC-PdNPs has been rapidly and effectively inhibited through the formation of Pd-I bond; thus, the active sites of PdNPs can be blocked by I − . Based on this specific inhibition by I − , a facile colorimetric assay has been performed for the detection of I − with an extremely low limit of detection (0.19 nM). Furthermore, the practicality of the proposed sensor also has been demonstrated in tap water, and the satisfactory recoveries were obtained. Our study not only demonstrated a novel Pd–based nanozyme but also provided guidance for I − sensing for environmental analysis, food inspection, and bio-detection.
Graphical abstract
研究领域:
纳米技术
·
分析化学
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环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 29
·7857-7868
·
IF 3.286
Luo Danqiu,Chen Huayun,Zhou Pei,Tao Han,Wu Yuangen
A novel colorimetric sensing platform based on the peroxidase activity of hemin regulated by oligonucleotide and pesticide was reported for the ultrasensitive and selective detection of isocarbophos. Oligonucleotides can accumulate on the surface of hemin in acid condition and temporarily inhibit its catalytic activity, which results in the loss of one electron of TMB molecule and produce the blue products. With the addition of isocarbophos, the pesticide molecules can interact with oligonucleotides to form some complexes, which relieve the inhibition of ssDNA to hemin and further enhance its catalytic activity. Thus, the TMB molecules are further oxidized to lose another electron and produce the yellow product in a few minutes, which has the characteristic absorption peak at 450 nm. The color change of the sensing system is related to the amount of isocarbophos, so this method can quickly discriminate whether the target pesticide exceeds the maximal residue limit just by naked eyes. To improve the performance of sensing platform, some important parameters like buffer condition and ssDNA have been investigated, and the peroxidase activity of hemin was further studied to verify the catalytic mechanism. The proposed sensing platform has a detection limit as low as 0.6 μg/L and displays good selectivity against other competitive pesticides. Moreover, the developed sensing platform also exhibits favorable accuracy and stability, indicating that it has potential applications in the detection of pesticide residues in agricultural products.
A novel colorimetric sensing platform based on oligonucleotides and pesticide regulation; the peroxidase catalytic activity of hemin was firstly reported for the ultrasensitive and selective detection of isocarbophos pesticide.
研究领域:
分析化学
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环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 24
·10873-10883
·
IF 2.7
Yunxia Shi,Hui Duan,Meiyan Du,Xia Kong,Ke Xue,Guang Lu,Qingyun Liu
In this work, 2(3),9(10),16(17),23(24)-octamethoxyphthalocyanine (Pc(OH)8) was first used to modify Cu/CuFe2O4(Cu/CFO) nanospheres, and Pc(OH)8–Cu/CFO nanocomposites with enhanced peroxidase mimetic enzyme activity was obtained compared to the performance of Cu/CFO alone. SEM, XRD, and XPS were used to study the surface morphology, phase composition and elemental analysis of the newly synthesized Pc(OH)8–Cu/CFO. Using TMB as the chromogenic substrate, Pc(OH)8–Cu/CFO accelerate the oxidation process of chromogenic substrate 3,3′,5,5′- tetramethylbenzidine (TMB) by hydrogen peroxide in three minutes. The catalytic oxidation mechanism of Pc(OH)8–Cu/CFO on TMB was comprehensively investigated through free radical and fluorescence probe experiments. Based on the excellent catalytic performance of the composite materials, a simple and fast colorimetric sensing platform for the colorimetric detection of H2O2 and glutathione (GSH) was constructed in the range of 0.6–5 mM and 2–10 μM with detection limits (LODs) of 0.35 mM and 0.55 μM, respectively. In addition, using Pc(OH)8–Cu/CFO colorimetric detection for GSH has a lower detection limit, good selectivity and high sensitivity, which is expected to become an ideal material for GSH colorimetric detection.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jing Chen,Jia Ge,Lin Zhang,Zhaohui Li,Lingbo Qu
In this work, we developed a highly sensitive and simple colorimetric method for glucose detection using poly(styrene sulfonate)/Pt-modified graphene nanosheets (PSS-GN-Pt) as peroxidase mimetics. First, in the presence of glucose, H 2 O 2 was generated as the main by-product of glucose oxidase (GOx)-catalyzed reaction. Then, the obtained H 2 O 2 triggered the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue color with the aid of PSS-GN-Pt nanocomposites as peroxidase mimetics. Via this method, as low as 9.28 × 10 −8 M glucose could be detected with a linear range from 2 × 10 −7 M to 1 × 10 −3 M. Besides absorbency measuring, the visual detection of glucose by naked eyes could also be realized very easily through the observable color changing from colorless to blue. In addition, this strategy was further used to determine the concentrations of glucose both in injection and serum samples with satisfying results. This proposed nanosystem holds great potential for diabetes mellitus research and can be easily expanded to the detection of various H 2 O 2 -involved analytes.
研究领域:
分析化学
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生物传感器
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材料科学
·
糖尿病研究
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Chun-Yan Zhang,Tong-Qing Chai,Guo-Ying Chen,Wei-Yi Zhang,Hao Zhang,Feng-Qing Yang
The peroxidase-like activity of adenine phosphate (AP) was investigated for the first time using chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Effects of different reaction conditions, including pH, temperature, AP concentration and incubation time on the catalytic property of AP, were investigated. The peroxidase-like activity of AP complies with Michaelis-Menten kinetics, and the calculated kinetic parameter ( K m , 0.038 mM) of AP catalysis exhibits higher affinity to H 2 O 2 than natural horseradish peroxidase and some of the other small-molecule peroxidase mimics. The radical scavenging results confirm that hydroxyl radical (•OH) is the active specie of catalytic process. Based on the excellent catalytic performance of AP, a facile colorimetric method for the H 2 O 2 and ascorbic acid detection with good sensitivity and selectivity was established at the linear ranges of 2.0–50.0 μM and 20.0–70.0 μM, respectively. In addition, the developed method was applied to detect H 2 O 2 and ascorbic acid in practical samples. In short, this work will not only broaden the understanding of biological function of AP, but also facilitate the development of new category of small-molecule enzyme mimics.
研究领域:
生物化学
·
分析化学
·
酶学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 22
·6611-6620
·
IF 4.478
Tang Yulian,Lv Xue,Gou Wenxin,Zhou Xuemei,Hao Junkai,Feng Jing,Qi Yuji,Hu Lei,Yan Zhengquan
Dopamine (DA) is an important neurotransmitter; however, any excess or deficiency of DA will cause several diseases in humans. To monitor DA efficiently and conveniently, a Ag nanozyme strengthened by bioactive folic acid (FA@AgNPs) was developed by homogeneous redox assembly. After the microstructure and performance were characterized in detail, it was noted that the proposed FA@AgNPs possessed superior peroxidase-like activity due to the ultra-small Ag nanoparticles and multiple amino, hydroxyl, and aromatic rings in FA. FA@AgNPs accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with a low Michaelis constant ( K m ) and high maximal reaction rate ( V max ). Importantly, the characteristic absorbance intensity of FA@AgNPs-TMB-H 2 O 2 at 652 nm ( A 652 ) was exclusively deteriorated in the presence of trace DA, accompanied by a visual color change from blue to colorless. Under the optimized conditions (pH 4.0, 300 μL 1.5 mM TMB, 300 μL 1.0 M H 2 O 2 and incubated for 30 min at room temperature), there expressed an excellent linear relationship between lg A 0 / A 652 and c DA from 1.0 ×10 −8 to 6.67×10 −6 mol/L with a low limit detection of 7.1×10 −10 mol/L (S/N=3). When applied for monitoring of DA in real fruit juice and pharmaceutical samples, the recovery was between 96.6% and 104.9%, with RSD less than 2.2%. The enhanced peroxidase-like activity of the FA@AgNP system and its selective recognition mechanism for DA are also proposed. Graphical abstract
研究领域:
纳米技术
·
生物化学
·
分析化学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Wei Wang,Ting Bao,Xi Zeng,Huayu Xiong,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, a novel and ultrasensitive electrochemical biosensor was constructed for DNA detection based on functionalized gold clusters/graphene nanohybrids (AuNCs/GR nanobybrids) and exonuclease III (Exo III)-aided cascade target recycling. By utilizing the capacity of GR as universal template, different metal nanoclusters including AuNCs/GR nanobybrids and PtNCs/GR nanohybrids were synthesized through convenient ultrasonic method. Exo III-aided cascade recycling was initiated by target DNA, generating the final cleavage product (S2), which acted as a linkage between capture probe and the functionalized metal nanoclusters/GR conjugates in the construction of the biosensor. The AuNCs/GR-DNA-enzyme conjugates acted as interfaces of enzyme-catalyzed silver deposition reaction, achieving DNA detection ranging from 0.02 fM to 20 pM with a detection limit of 0.057 fM. In addition, PtNCs/GR-DNA conjugates presented peroxidase-like activity and the functionalized PtNCs/GR nanohybrids-based electrochemical biosensor also realized DNA detection by catalyzing the 3,3′,5,5′-tetramethylbenzidine-hydrogen peroxide (TMB-H 2 O 2 ) system to produce electrochemical signal. This metal clusters/GR-based multiple-amplified electrochemical biosensor provided an universal method for DNA detection.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Meng Xu,Yimeng Li,Xiaopeng Hu,Shuai Zhang,Yiwei Liu
A smartphone-assisted colorimetric aptasensor was constructed for the detection of ochratoxin A (OTA) utilizing Au/CeO₂/rGO nanohybrid. The sensor integrates aptamer-functionalized Au/Fe 3 O 4 (Apt/Au/Fe 3 O 4 ) as recognition elements. The complementary-chain-modified Au/CeO₂/rGO (cDNA/Au/CeO₂/rGO) act as nanozyme catalyst for signal amplification through the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored ox-TMB. The detection mechanism relies on competitive binding between OTA and cDNA/Au/CeO 2 /rGO to Apt/Au/Fe 3 O 4 . Upon OTA introduction, OTA preferentially binds to Apt/Au/Fe 3 O 4 . These Apt/Au/Fe 3 O 4 are magnetically separated and introduced into the cDNA/Au/CeO 2 /rGO suspension. Unbound Apt/Au/Fe 3 O 4 interacts with cDNA/Au/CeO 2 /rGO. After magnetic separation, the residual cDNA/Au/CeO 2 /rGO concentration in supernatant exhibits a correlation with the OTA concentration. This design effectively eliminates potential interference from the intrinsic color of coffee, significantly improving the accuracy of colorimetric detection. The aptasensor demonstrated superior selectivity towards OTA with a detection limit of 27.3 pg mL −1 . This specificity was validated against interference, with recovery rates of 102.1–117.4 % in coffee, as confirmed by ELISA.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
生物传感器
·
毒素检测
·
适配体技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）