JOURNAL OF ANIMAL SCIENCE
·
2024
·
IF 2.7
Ma Zifeng,Li Qiao,Xu Hongmei,Li Yueyue,Wang Shujin,Xiong Yan,Lan Daoliang,Li Jian,Xiong Xianrong,Fu Wei
Zearalenone (ZEN), a mycotoxin from Fusarium fungi, impairs fertility and milk production in female animals, however, the mechanisms remain poorly understood. Using the bovine mammary epithelial cells (MAC-T) as the model, this study investigated the impacts of ZEN on programmed cell death (PCD) and milk fat synthesis, and explored the underlying mechanism. We found that 10 ng/mL prolactin (PRL) notably enhanced the differentiation of MAC-T cells, promoting the expression of genes related to the synthesis of milk fat, protein, and lactose. Next, the toxic effects of different doses of ZEN on the differentiated MAC-T with PRL treatment were determined. 10 μM and 20 μM ZEN significantly reduced cell viability, induced oxidative stress, and triggered PCD (e.g. apoptosis and necrosis). Notably, ZEN exposure downregulated the mRNA/protein levels of critical factors involving in milk fat synthesis by disrupting the AKT-mTOR-PPARγ-ACSL4 pathway. Interestingly, melatonin (MT), known for its antioxidant properties, protected against the above ZEN-induced effects by enhancing the binding of PPARγ to the promoter regions of ACSL4, which led to the upregulated expression of ACSL4 gene. These results underscored the potential of MT to mitigate the adverse effects of ZEN on mammary cells, highlighting a way for potential therapeutic intervention.
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高影响因子
Toxins
·
2023
·Vol. 15
·期 5
·337
·
IF 4.2
Baoxia Ni,Jin Ye,Zhihong Xuan,Li Li,Xiangrui Wen,Zongwang Li,Hongmei Liu,Songxue Wang
Sample pretreatment is a vital step in the detection of mycotoxins, and traditional pretreatment methods are time-consuming, labor-intensive and generate much organic waste liquid. In this work, an automatic, high-throughput and environmentally friendly pretreatment method is proposed. Immunomagnetic beads technology and dispersive liquid–liquid microextraction technology are combined, and the zearalenone in corn oils is directly purified and concentrated under the solubilization effects of surfactant. The proposed pretreatment method allows for the batch pretreatment of samples without pre-extraction using organic reagents, and almost no organic waste liquid is produced. Coupled with UPLC-FLD, an effective and accurate quantitative detection method for zearalenone is established. The recovery of spiked zearalenone in corn oils at different concentrations ranges from 85.7 to 89.0%, and the relative standard deviation is below 2.9%. The proposed pretreatment method overcomes the shortcomings of traditional pretreatment methods and has broad application prospects.Keywords:immunomagnetic beads;dispersive liquid liquid microextraction;pretreatment;zearalenone;corn oils;UPLC-FLDKey Contribution:An automatic and eco-friendly pretreatment method of zearalenone (ZEN) in corn oils was developed based on immunomagnetic beads with dispersive liquid–liquid microextraction technology. The proposed pretreatment method, coupled with UPLC-FLD, met the accuracy and sensitivity detection requirements of ZEN in corn oils.
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TALANTA
·
2020
·
IF 5.339
Xiaopeng Hu,Caiyun Wang,Meng Zhang,Faqiong Zhao,Baizhao Zeng
Accurate and early diagnosis of zearalenone (ZEN) is particularly significant to the food safety. Herein, we propose an ionic liquid assisted self-assembly molecular imprinting strategy for ZEN based on ionic liquid functionalized boron-doped ordered mesoporous carbon -gold nanoparticles composite (BOMC-IL-Au NPs). During the composite synthesis, increased well-dispersed and uniform Au NPs are deposited on the surface of IL modified BOMC, due to the strong electrostatic interaction between AuCl 4 − and positively charged IL. For molecular imprinting, the BOMC-IL-Au NPs/GCE is immersed into p-aminothiophenol (p-ATP) solution and template solution in turn. Thus, the mercapto group contained p-ATP self-assembles on the Au NPs. Subsequently, the template molecules self-assemble onto the composite to form dense template layer, because of the hydrophobic interaction, π-π and hydrogen bond between template and IL/or p-ATP. After electropolymerization, the template layer is embedded into the p-ATP polymer membrane and produces lots of imprinting sites. Hence, the obtained sensor exhibits high sensitivity and selectivity. Under the optimal conditions, zearalenone can be quantified from 5 × 10 −4 to 1 ng mL −1 with the low detection limit of 1 × 10 −4 ng mL −1 , by using [Fe(CN) 6 ] 3-/4- probe and square wave voltammetry . This strategy can also be employed to construct sensors for the detection of other substances.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-8
·
IF 5.479
Fang Dandan,Zhang Shupei,Dai Hong,Li Xiuhua,Hong Zhensheng,Lin Yanyu
An ultrasensitive competitive-type electrochemiluminescence immunoassay for the mycotoxin zearalenone is described. The method is based on the use of (a) a mimotope peptide that was selected from a phage displayed peptide library and used to substitute ZEN for designing the competitive assay; (b) NiFe 2 O 4 nanotubes with large specific surface area loaded with the ECL probe Ru(bpy) 3 2+ ; and (c) poly(vinylpyrrolidone) (PVP)-assisted synthesis of TiO 2 mesocrystals that acts as the sensing platform and support for antibody immobilization. Under the optimized conditions and at an ECL working potential of 1.1 V, a linear response is found for ZEN in the 0.1 to 1.0 × 10 −5 ng·mL −1 concentration range with a detection limit as low as 3.3 fg·mL −1 .
An ultrasensitive competitive-type electrochemiluminescence (ECL) immunosensor based on mimotope peptide was constructed for the detection of Zearalenone.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-10
·
IF 5.479
He Baoshan,Yan Xiaohai
The authors constructed a voltammetric zearalenone (ZEN) aptasensor based on use of porous platinum nanotubes, gold nanoparticles (p-PtNTs/AuNPs) and thionine (Thi) labelled graphene oxide (GO). The p-PtNTs were synthesized in-situ based on tellurium nanowires as sacrificial templates. Subsequently, thiol-modified aptamers were self-assembled on the AuNPs that had been electrodeposited on the surface of the modified electrode. The presence of p-PtNTs on the electrode increases the loading with AuNPs and aptamers. It also warrants that the Thi-labelled GO can be assembled onto the aptamer via π interactions. In the presence of ZEN, it will be bound by the aptamer. The GO/Thi conjugate will be released from the aptamer, and this causes a decrease in Thi current. Under the optimal conditions and at a typical working potential of −0.22 V (vs. Ag/AgCl), the method has a linear range that covers the 0.5 pg·mL −1 to 0.5 μg·mL −1 ZEN concentraion range and a lower detection limit of 0.17 pg·mL −1 .
Voltammetric zearalenone aptasensor based on use of porous platinum nanotubes/gold nanoparticles and thionine labelled graphene oxide was fabricated for the detection of zearalenone.
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FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Tongtong Shen,Yufan Miao,Chenchen Ding,Wentao Fan,Shuhui Liu,Yanan Lv,Xiaona Gao,Marthe De Boevre,Liping Yan,Sheila Okoth,Sarah De Saeger,Suquan Song
Zearalenone (ZEA) can widely contaminate crops and agricultural products. The ingestion of ZEA-contaminated food or feed affects the integrity and functions of the intestines. In this study, we aimed to find the potential protective mechanism against ZEA ingestion. We found that ZEA induced cell death in IPEC-J2 cells. Meanwhile, the cytoprotective autophagy was activated in ZEA-treated cells. Further studies demonstrated that a p38/MAPK inhibitor down-regulated autophagy and increased cell death compared to those of the controls. Furthermore, ZEA could induce the accumulation of ROS , and eliminating ROS with NAC resulted in a decline in cell death, p38/MAPK phosphorylation, and the expression of LC3-II compared to those of ZEA-group. In addition, cytochrome P450 reductase (CYPOR) was significantly increased in ZEA-treated cells compared to that in the controls, and an inhibitor of CYPOR decreased ROS levels and mitigated cell death compared to those of the ZEA-group. More importantly, we found that blocking both p38/MAPK signalling and autophagy could enhance CYPOR expression and elevate ROS levels. Overall, our study indicated that the p38/MAPK pathway could activate protective autophagy in response to the CYPOR-dependent oxidative stress that was induced by ZEA in IPEC-J2 cells.
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JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Xue Li,Meng-Yao Wang,Yu Wang,Wen-Zhi Yang,Cheng-Xiong Yang
Efficient enrichment of trace zearalenone (ZEN) from the complex traditional Chinese medicine (TCM) samples is quite difficult, but of great significance for TCM quality control. Herein, we reported a novel magnetic solid phase extraction (MSPE) strategy for ZEN enrichment using the amino- and hydroxyl dual-functionalized magnetic microporous organic network (Fe 3 O 4 @MON-NH 2 -OH) as an advanced adsorbent combined with the high-performance liquid chromatography (HPLC) determination. Efficient extraction of ZEN was achieved via the possible hydrogen bonding, hydrophobic, and π-π interactions between Fe 3 O 4 @MON-NH 2 -OH and ZEN. The adsorption capacity of Fe 3 O 4 @MON-NH 2 -OH for ZEN was 215.0 mg g −1 at the room temperature, which was much higher than most of the reported adsorbents. Under the optimal condition, the developed Fe 3 O 4 @MON-NH 2 -OH-MSPE-HPLC method exhibited wide linear range (5–2500 μg L −1 ), low limits of detection (1.4–35 μg L −1 ), less adsorbent consumption (5 mg), and large enhancement factor (95) for ZEN. The proposed method was successfully applied to detect trace ZEN from 10 kinds of real TCM samples. Conclusively, this work demonstrates the Fe 3 O 4 @MON-NH 2 -OH can effectively extract trace ZEN from the complex TCM matrices, which may open up a new way for the application of MONs in the enrichment and extraction of trace contaminants or active constituents from the complex TCM samples.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoya Bi,Libo Li,Lijun Luo,Xiaohong Liu,Jiamin Li,Tianyan You
Semiconductor quantum dots (QDs) and tungsten disulfide nanosheets (WS 2 NTs) have been widely studied in photocatalysis and photoelectrochemistry as representative electron donor–acceptor pairs but rarely in fluorescence sensing. In this study, we investigated the effect of WS 2 NTs on the fluorescence signal of cadmium telluride (CdTe) QDs by grafting an aptamer as a bridge between them. The corresponding quenching mechanism was systematically explored, and results described the photoinduced electron transfer (PET) from excited CdTe QDs to WS 2 NTs. Based on these results, a novel ratiometric fluorescence aptasensor was developed for zearalenone (ZEN) determination by innovatively introducing exonuclease I to digest the aptamer of ZEN and control the PET between CdTe QDs and WS 2 NTs. The designed aptasensor exhibited an acceptable linear range and detection limit (0.1 pg mL −1 ), and superior accuracy and selectivity. This ratiometric fluorescence aptasensor was also used to monitor ZEN in rice and corn flour.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Qingyue Li,Mengtian Chen,Jieling Ren,Sichang Li,Tong Bu,Yang Wang,Xianqing Huang,Lianjun Song,Liang Liu,Ruifeng Luo,Yexuan Mao,Xiya Zhang
The food samples with high-fat content could induce matrix effects and interfere with the accuracy of lateral flow immunoassays (LFA), while deep eutectic solvents (DESs) as the innovative extraction solution had great potential to decrease this effect by rational design. First, 24 samples were evaluated for the detection performance of LFA using zearalenone (ZEN) as the target, and we found that black sesame, pecan, cashew, and pistachio with high-fat content could seriously affect recoveries and limit of detection (LOD), generating high matrix effects. To address this issue, six DESs with different polarities were designed by arrangement of two hydrogen bond donors and three hydrogen bond acceptors. DES-4, composed of betaine and ethylene glycol with a molar ratio of 1:4, displayed a lower molecular polarity index, which was similar to ZEN and possessed significant differences with fatty acids. Subsequently, DES-4 was applied to LFA for ZEN detection in these four high-fat matrices. After centrifugation, 70.5 %-92.3 % of fat in food matrices was transferred to the upper layer, and the fat content of the detection phase and food matrices phase by DESs extraction was 77.0–290.2 mg, obviously lower than that by acetonitrile/water extraction. Moreover, the recoveries ranged from 82.01 % to 114.26 % after diluting 50–70 times, and the LODs were 1.72–4.06 ng/mL, which were 1.4–4.6 times lower than that of acetonitrile/water extraction. Hence, DESs were considered as promising extraction reagents to reduce the high fat-induced matrix effects, which may be broadened to other target detection.
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Food Bioscience
·
2026
·
IF 6.2
Chenming Lei,Yilian Yin,Daozhi Yang,Shun Yang,Hui Fei
Low-dose ZEN caused apparent growth enhancement with subclinical gut-liver injury. • High-dose ZEN inhibited growth and caused severe gut-liver damage. • ZEN accumulated in tissues: liver > intestine > muscle. • High-dose ZEN induced gut dysbiosis and hepatic metabolic disorders. • ZEN perturbed RAS/glycerophospholipid pathways; muscle residues pose exposure risk. Zearalenone (ZEN), an estrogen-like mycotoxin, represents an emerging hazard in aquatic food supply chains, as contaminated aquafeeds transfer ZEN to farmed fish and then to consumers. Juvenile largemouth bass ( Micropterus salmoides ), a widely consumed aquaculture species, were subjected to subchronic dietary ZEN exposure (0, 100, and 1000 μg kg −1 feed) for 28 days. Integrated 16S rRNA microbial profiling and untargeted hepatic metabolomics characterized dose-dependent toxic responses and food-chain transfer risk. ZEN exhibited tissue-specific accumulation (liver > intestine > muscle); muscle ZEN residue reached 2.4 μg kg −1 under high-dose exposure. Low-dose ZEN elicited apparent growth enhancement accompanied by subclinical hepatic and intestinal injury, whereas high-dose ZEN induced growth retardation, hepato-intestinal histopathological lesions, intestinal tight-junction gene down-regulation, and elevated serum alanine aminotransferase and aspartate aminotransferase. High-dose ZEN triggered gut microbiome dysbiosis: commensal genera ( Mycoplasma , Rhodococcus ) were depleted while pathobiont Sphingomonas bloomed. Key hepatic metabolic pathways including ABC transporters, renin-angiotensin system, and glycerophospholipid metabolism were perturbed. Microbiome-metabolome integrative analysis revealed a dose-dependent shift from positive toward predominantly negative microbe-metabolite correlations, indicating collapse-like reorganization of host-microbe associations. Notably, even a dietary ZEN concentration around the current regulatory limit (nominal 100 μg kg −1 ; measured 140.6 μg kg −1 ) elicited subclinical intestinal injury in this species. Human dietary exposure assessment indicated low acute risk via consuming ZEN-contaminated largemouth bass, yet long-term cumulative risk for vulnerable subgroups cannot be ignored. Our foodomics findings provide hazard-characterization data that may inform, but do not by themselves establish, future re-evaluation of ZEN maximum limits in aquafeeds to safeguard aquatic food safety and public health.
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2024
·
IF 1.5
Song Lixin,Zhang Jian,Wang Mingyu,Huang Zhipeng,Zhang Yunxia,Zhang Xing,Liang Yutao,He Juan
A novel approach for the simultaneous separation of zearalenone (ZEN) and four types of aflatoxins (AFB1, AFB2, AFG1 and AFG2) from rice samples was presented. This approach utilized modified MIL-101(Cr)-NH2 as core, with molecularly imprinted polymers (MIPs) serving as the shell. The MIL-101(Cr)-NH2 was prepared via ring-opening reaction, while the imprinted polymers were synthesized using warfarin and 4-methylumbelliferyl acetate as co-pseudo template, ethylene glycol dimethacrylate as the cross-linker and azobisisobutyronitrile as initiator. The resulting co-pseudo-template-MIPs (CPT-MIPs) were thoroughly characterized and evaluated. Adsorption studies demonstrate that the adsorption process of CPT-MIPs follows a chemical monolayer adsorption mechanism, with imprinted factors ranging from 1.24 to 1.52 and selective factors ranging from 1.29 to 1.52. Self-made columns were prepared, and the method for separation was developed and validated. The limit of detections ranged from 0.12 to 2.09 μg/kg, and the limit of qualifications ranged from 1.2 to 6.25 μg/kg. To assess the reliability of the method, ZEN and AFs were spiked at three different levels, and the recoveries ranged from 79.53 to 94.58%, with relative standard deviations of 2.90–5.78%.
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TALANTA
·
2018
·
IF 4.244
Sobia Niazi,Xiaole Wang,Imran Pasha,Imran Mahmood Khan,Sen Zhao,Muhammad Shoaib,Shijia Wu,Zhouping Wang
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by fungi on stored grains. The earlier detection methods used for ZEN rely on expensive equipment, time-consuming sample preparation and temperature sensitive antibodies. The current work, proposed a novel strategy based on ZEN aptamer labeled with amine-functionalized magnetic nanoparticle (MNPs) as a capture probe and time-resolved fluorescence (TRFL) nanoparticles labeled with complementary DNA (cDNA) as a signal probe. Under the optimized conditions, TRFL intensity at 544 nm was used to measure ZEN (R 2 = 0.9920) in the range of 0.001–10 ng mL −1 and limits of detection (LOD) for proposed method was 0.21 pg mL −1 . The specificity of bioassay was also determined by using other mycotoxins (OTA, AFB 2 , DON and Patulin) and results showed that the aptamer are specific to recognize only ZEN. The analytical applications of the present bioassay in maize and wheat samples were also examined and results were compared with existing methods. Based on these findings, it is suggested to use current rapid and simple bioassay for the determination of ZEN in food and agricultural products.
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