APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
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IF 14.229
Yu-Xuan Xiao,Jie Ying,Ge Tian,Yong Tao,Hao Wei,Si-Yu Fan,Ze-Hao Sun,Wan-Juan Zou,Jie Hu,Gang-Gang Chang,Weihua Li,Xiao-Yu Yang,Christoph Janiak
The strong Pt-carbon interaction is a very promising way to high-performance design of electrocatalysts. However, the heavy d-π overlap, a high strength of Pt-C bonding, in sp 2 bonded graphitic carbons with low level of defects is rarely reported. Herein, PtPd nanoparticles and graphitic carbon nanofibers (PtPd/CNF) have been integrated via heavy d-π overlap by an interfacial wettability approach and their formation mechanism has also been fully elucidated. The features of strong d-π overlap and high dispersion of metals offer PtPd/CNF with enhanced ORR activity (5.8 folds of specific activity than commercial Pt/C), and excellent structural stability and electrocatalytic durability. A new “heavy d-π overlap” concept, characteristics, and mechanism are proposed at an atomic-/nanoscale to clarify the generation of the predictive interaction as well as the interface charge transfer. It is believed that the study on heavy d-π overlap sheds new light on the fundamental aspects of the nature of Pt-C interaction.
研究领域:
材料科学
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电化学
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纳米技术
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DOI
高影响因子
CATALYSIS LETTERS
·
2020
·Vol. 151
·期 2
·338-343
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IF 2.482
Shi Hexian,Zhang Linfeng,Wu Yudong,Yu Rui,Peng Yang,Wang Yantao,Li Teng,Yang Weiran
Abstract Glucose and its derivatives are valuable and sustainable carbon sources that can be converted into varieties of value-added chemicals. In this study, d -glucaric acid was selectively converted to 3-iodoadipic acid (3-IAA) by hydrodeoxygenation in HI-Rh/C-H 2 system. During this process, HI was a reducing reagent, which can be regenerated in situ by metal catalyzed hydrogenation. The reaction pathway was proposed based on the intermediates identification. Under the optimal conditions, 67.2% yield of 3-IAA was obtained at 130 ℃ for 4 h. As a platform molecule, 3-IAA can be further converted to varieties of building block efficiently, such as adipic acid (AA) and 3-hydroxyadipic acid (3-HAA). Graphic Abstract
研究领域:
化学工程
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可再生资源转化
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DOI
高影响因子
Journal of Materials Chemistry A
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2023
·Vol. 12
·期 7
·3967-3976
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IF 11.9
Surong Zheng,Shiwei Yu,Zaka Ullah,Lei Liu,Ledi Chen,Houliang Sun,Mingliang Chen,Liwei Liu,Qi Li
All-solid-state lithium-ion batteries have great scope in the development of advanced secondary batteries owing to their high energy density and nonflammability. However, high contact resistance at the solid/solid (electrode/electrolyte) interface and the possible existence of side reactions limit their large-scale applications. In this study, a π-d conjugated coordination structure was established between a cathode and electrolyte: 2,5-dihydroxy-1,4-benzoquinone (DHBQ) was the cathode and polyvinylidene fluoride-based Li0.33La0.557TiO3 (PVDF-LLTO) polymer was the solid-state electrolyte. First-principle calculations and performed experiments showed that CO and O–H groups in DHBQ can be coordinated with La3+ in LLTO, and this π-d conjugate coordination structure strengthens the contact interface between the electrode material and solid electrolyte, which further increases the cycling life and durability of all-solid-state batteries.
研究领域:
材料科学
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能源技术
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 17
·8029-8036
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IF 3.925
Qingju Liu,Saixue Tang,Hui Wang,Ping Han
The current luminescent metal–organic framework (MOF) based fluorescence detection mostly focuses on achiral molecules. The stereospecific recognition and determination of MOF-based detection remain challenging. A novel luminescent terbium-based MOF (Tb-MOF) based biosensing system with excellent performance for stereoselective detection of D-amino acids (D-AAs) was introduced in this paper. D-Amino acid oxidase catalyzes stereospecific oxidative deamination of D-AAs, producing H2O2. The generated H2O2 effectively quenches the fluorescence of Tb-MOF. Under optimum conditions, the D-AAs were detected with excellent efficiency. The linear working range was 0.5–100 μM for D-Alanine (D-Ala) and 0. 5–5 μM for D-Proline (D-Pro), respectively. The limit of detection (Ksv detection) for D-Ala and D-Pro was 0.09 μM and 0.14 μM, respectively. The detection can be performed within 30 min with high selectivity and long-term stability. The fluorescent sensing platform required simple fabrication and operation and was free from complicated reaction processes and expensive reagents. The present sensing system was successfully applied in D-AA detection in human serum with good recovery, indicating its potential application in detection in biological samples.
研究领域:
分析化学
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生物技术
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材料科学
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DOI
高影响因子
Food Bioscience
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2024
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IF 4.8
Han Zhang,Zhen Sun,Xinling Du,Qianqian Cheng,Fuchun Ji,Zishen Nie,Julei Zhan,Zhouping Wang,Aitao Li,Irina Delidovich,Yu Xia
d- Allulose is a rare sugar with multiple physiological functions whose application has been restricted by low conversion rate, weak thermostability of the used enzymes, and complex production. In this study, a simplified multienzyme cascade strategy was designed for biosynthesis of d- allulose from d- glucose, and a detailed investigation of the crucial enzyme allulose 6-phosphate phosphatase was conducted. Under optimized conditions, the yield of d- allulose reached 4.12 ± 0.14 and 80.20 ± 3.20 mmol/L using 10.0 and 200.0 mmol/L d- glucose as substrate, respectively. Furthermore, the influence of various factors on the cooling crystallization of d- allulose was investigated, and the crystallization process was optimized. Consequently, a crystallization recovery rate of 77.43% ± 2.50% and a purity of 98.70% ± 0.05% were achieved. Moreover, the obtained crystals of d -allulose were characterized by XRD, TGA-DSC, and optical microscopy.
研究领域:
生物化学
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食品科学
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DOI
高影响因子
BIOORGANIC CHEMISTRY
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2025
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IF 4.7
Ruobin Sun,Qipeng Yan,Wenhao Deng,Pengcheng Chen,Dan Wu,Pu Zheng
Achieving enzyme catalysis at high substrate concentrations is a substantial challenge in industrial biocatalysis, and the role of glycosylation in post-translational modifications that modulate enzyme substrate inhibition remains poorly understood. This study provides insights into the role of N -glycosylation in substrate inhibition by comparing the catalytic properties of d -lactonohydrolase ( d -Lac) derived from Fusarium moniliforme expressed in prokaryotic and eukaryotic hosts. Experimental evidence indicates that recombinant d -Lac expressed in Pichia pastoris ( Pp Lac-WT) exhibits higher hydrolysis rates at a substrate concentration of 400 g/L, with reduced substrate inhibition and enhanced stability compared to the recombinant d -Lac expressed in Escherichia coli ( Ec Lac-WT). Mutant Pp Lac-M1 achieves a conversion rate of 40 % at a substrate concentration of 400 g/L, with a space–time yield of d -pantoic acid reaching 91.1 g/L/h. Proteomics analysis reveals that residues N29 and N278, located approximately 10–20 Å from the active site undergo N -glycosylation in Pp Lac-WT. Using microsecond-scale molecular dynamics simulations and Markov state models, we elucidate the effects of glycosylation on the conformational flexibility of two key loops at the entrance of the binding pocket. Specifically, the loops in Pp Lac-WT can transition between open and closed states, whereas those in Ec Lac-WT tend to remain open. In high substrate concentration conditions, the open state causes congestion, leading to substrate inhibition. Shortest-path map analysis confirms that substrate entry is dynamically controlled by residue N29 on the loops surrounding the active site. Our findings enhance the understanding of the effects of glycosylation on enzyme conformational dynamics and provide insights into mitigating inhibition at high substrate concentrations.
研究领域:
生物化学
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酶工程
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结构生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Jing Sun,Zaixu Wang,Yongjin Dai,Moran Zhang,Xinyi Pang,Xiangfei Li,Yingjian Lu
Molds and mycotoxins pose severe threats to health. Bacillomycin D (BD) can effectively inhibit mold growth. Attapulgite (ATP) can provide a good carrier for antimicrobial agents. Natural ATP was acid-modified to obtain H-ATP. It was used to load BD to obtain a novel composite material (H-ATP-BD). The results showed H-ATP had better adsorption performance than ATP. BD was adsorbed up to 93.13 % by adding 30 mg H-ATP and stirring at 40 ℃ for 120 min. Fourier transform infrared spectra (FTIR), size and zeta potential, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) results confirmed successful loading of BD onto H-ATP. The composite showed good inhibition of Aspergillus and adding 0.6 % H-ATP-BD composite was effective in removing 89.06 % of aflatoxin B 1 (AFB 1 ) at 50 °C. Model fitting indicated that AFB 1 removal was a spontaneous exothermic reaction. This research will lay the foundation for the development of efficient and green antimicrobial and toxin-reducing materials.
研究领域:
食品科学
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微生物学
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材料科学
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DOI
PubMed
高影响因子
Green Energy & Environment
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2020
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IF 6.395
Pengfei Liu,Christian Marcus Pedersen,Jiaojiao Zhang,Rui Liu,Zhenzhou Zhang,Xianglin Hou,Yingxiong Wang
Ternary deep eutectic solvents (TDESs) comprising choline chloride (ChCl), glycerol and l -arginine were synthesized as catalysts and solvents for the conversion of d -glucosamine (GlcNH 2 ) into deoxyfructosazine (DOF). The interactions between these three components in the prepared TDESs were studied by 1 H-, 35 Cl-NMR spectra and 1 H diffusion-ordered spectroscopy (DOSY) measurements. The chemical shift changes of active hydrogen in the 1 H-NMR spectra of TDES system and widening of signals in the 35 Cl-NMR spectra confirmed the hydrogen bonding interaction between the components, which was further supported by the decrease of diffusion coefficients ( D ) of the TDES components according to 1 H DOSY measurements. The influences of reaction temperature and l -arginine content in the TDESs on the yield of DOF were also studied. The experimental results have shown that when the molar ratio of ChCl, glycerol, and l -arginine was 1:2:0.1, DOF was the major product with a yield of 22.6% at 90 °C for 120 min. The chemical shift titration indicated that the carboxyl group of l -arginine in the TDES is the catalytical active site, so the mechanism of the catalytic reaction between GlcNH 2 and the TDES was proposed. Moreover, a reaction intermediate, dihydrofructosazine, was identified in the self-condensation reaction of GlcNH 2 by an in situ 1 H NMR technique.
研究领域:
生物化学
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催化化学
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DOI
高影响因子
Biology-Basel
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2025
·Vol. 14
·期 3
·226
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IF 3.5
Zonghan Jiang,Leisheng Sun,Yuanyuan Li,Haoyu Li,Yu Fu,Jiyun Li,Zhiliang Sun
Simple SummaryEscherichia coliis a ubiquitous pathogen, with certain strains causing severe diarrhea and intestinal illnesses, which pose significant health risks to both children and animals. As antimicrobial resistance continues to rise, traditional antibiotics are increasingly ineffective, highlighting the urgent need for novel therapeutic strategies. In this study, we investigated the antimicrobial peptide D-CONGA-Q7, aiming to evaluate its efficacy and underlying mechanism against drug-resistantE. colistrains. Our results demonstrate that D-CONGA-Q7 effectively disrupts bacterial cell membranes, leading to bacterial death, and exhibits comparable activity against polymyxin- and meropenem-resistant strains. In vivo experiments revealed that D-CONGA-Q7 significantly alleviated intestinal inflammation, reduced inflammatory cytokine levels, and promoted the repair of damaged intestinal tissues. Furthermore, D-CONGA-Q7 displayed good stability. These findings suggest that D-CONGA-Q7 not only represents a promising new approach to combat drug-resistant bacterial infections but also holds potential for clinical application.Escherichia coli(E. coli) is a zoonotic bacterium widespread in the environment, highly transmissible, and responsible for significant economic losses and millions of cases of illness annually. The rise of multidrug-resistant (MDR) strains has rendered last-line antibiotics such as polymyxin and meropenem ineffective, making the development of new antibiotics urgent. Although D-CONGA-Q7 has broad-spectrum bactericidal activity, its underlying mechanism remains poorly understood. In this study, we used in vitro and in vivo experiments to demonstrate that D-CONGA-Q7 effectively kills both antibiotic-sensitive and multidrug-resistant strains ofE. coli. D-CONGA-Q7 disrupts the cell membranes of Gram-negative bacteria, and the treatment ofE. colistrainLN175with D-CONGA-Q7 resulted in a significant up-regulation of theMlacgene, suggesting that D-CONGA-Q7 may interact with phospholipids in the cell membrane. Furthermore, in treatingK88-induced bacterial enteritis in the small intestine, D-CONGA-Q7 significantly reduced intestinal inflammation. In conclusion, this study provides a novel approach to combat drug-resistantE. coli.
研究领域:
微生物学
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药理学
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传染病学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Qi Wang,Lingfang Li,Tingxuan Wu,Xiangpeng Kong,Qingguo Ma,Chunlei Ma
Taking consideration of metal-induced fluorescence quenching and excellent coordination effect of D-penicillamine (D-PA), a graphene quantum dots (GQDs)-based fluorescent switch for D-PA detection was designed and established firstly with the help of lead ions . GQDs obtained from citric acids made them rich in carboxyl and hydroxyl groups , giving GQDs the ability to combine with lead ions. As anticipated, the fluorescence intensity was quenched by Pb 2+ through electron transfer process. Further, the addition of D-PA effectively recovered the fluorescence due to the departure of Pb 2+ from GQDs aroused by the strong coordination between D-PA and Pb 2+ . Thus, a fluorescent switch was activated for D-PA detection. The fluorescence recovery efficiencies were found to be proportional to the concentration of D-PA in the range of 0.6–50 μmol L − 1 and the detection limit was 0.47 μmol L − 1 . The real sample detection was performed in human urea sample and satisfactory recoveries of 96.84%–102.13% were obtained. The GQDs-Pb 2+ based fluorescent switch sensing method was firstly established with low detection limit and wide linear range, making it a supplement and improvement for D-PA detection.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Yifan Chen,Juan Qiao,Qianrong Liu,Mingming Zhang,Li Qi
Papain-stabilized gold nanoclusters ( [email protected] ) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of [email protected] could be quenched in the addition of Cu 2+ . Subsequently, a unique fluorescent probe based on [email protected] 2+ complex has been constructed for sensitive, selective and “turn-on” detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 μM to 2.0 mM (r 2 = 0.991) with the detection limit of 5.0 μM. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent [email protected] 2+ complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs.
研究领域:
分析化学
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生物医学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Guoguang Wei,Alei Zhang,Kequan Chen,Pingkai Ouyang
This study presents an efficient pretreatment of crayfish shell using high pressure homogenization that enables N -acetyl- d -glucosamine (GlcNAc) production by chitinase. Firstly, the chitinase from Serratia proteamaculans NJ303 was screened for its ability to degrade crayfish shell and produce GlcNAc as the sole product. Secondly, high pressure homogenization, which caused the crayfish shell to adopt a fluffy netted structure that was characterized by Scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), was evaluated as the best pretreatment method. In addition, the optimal conditions of high pressure homogenization of crayfish shell were determined to be five cycles at a pressure of 400 bar, which achieved a yield of 3.9 g/L of GlcNAc from 25 g/L of crayfish shell in a batch enzymatic reaction over 1.5 h. The results showed high pressure homogenization might be an efficient method for direct utilization of crayfish shell for enzymatic production of GlcNAc.
研究领域:
生物化学
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废弃物利用
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酶工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）