WATER SCIENCE AND TECHNOLOGY
·
2017
·Vol. 77
·期 3
·706-713
·
IF 1.197
Jiang Yu,Yang Kai,Deng Tao,Ji Bin,Shang Yu,Wang Hongyu
A halotolerant yeast strain of Candida sp. was purified for phenol biodegradation and was immobilized in alginate and nano-SiO 2 . The concentration of nanoscale SiO 2 was optimized and phenol degradation performance with different initial phenol concentrations was evaluated. Three common kinetic models were used to correlate the experimental data. The effects of pH and salinity on phenol biodegradation were also investigated. It was found that 1.0% (w/v) was the optimal nano-SiO 2 concentration and the immobilized cells had a better phenol removal performance compared to free cells. More than 99% of 600 mg l −1 phenol was removed by the immobilized strains within 48 h. The immobilized cells also showed highest phenol degradation rates when pH and salinity were 6.5 and 0%, respectively. The high removal efficiency of phenol in reusability tests indicated the promising application of the immobilized Candida strain in phenol degradation under hypersaline conditions over a long period.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Zhou Li,Wenzhuo Zhao,Shouze Li,Dongyan Xu,Wonjoon Choi,Run Hu
A new hydrogel with gradient immobilized anions • Restricts anion thermodiffusion and boosts cation entropy • Giant Seebeck coefficient of 21.5 mV/K • Remarkable thermoelectric figure of merit ZT of 0.645 Ionic thermoelectric (i-TE) materials, which convert heat into electricity via the Soret effect, have attracted considerable interest due to their high thermopower and excellent flexibility. Restricting the thermal diffusion of either cations or anions has been demonstrated as an effective strategy to enhance thermoelectric performance; however, existing studies remain largely limited to systems with uniform concentration distributions. In this work, we develop a P-type thermoelectric hydrogel, denoted as PVA/PPy/GF@5.00%SDBS, which achieves a giant Seebeck coefficient of 21.5 mV·K −1 , representing 2.5-fold and 7-fold enhancements over uniformly immobilized and pristine systems, respectively. This exceptional performance is enabled by a gradient immobilization strategy that restricts anion diffusion while simultaneously increasing the Eastman entropy associated with cation transport. Collectively, these findings elucidate the role of ion immobilization in modulating the Soret effect and establish a new paradigm for controlling ion migration in i-TE systems.
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LANGMUIR
·
2026
·Vol. 42
·期 30
·21834-21845
·
IF 4.4
Li Bingbing,Lin Guoguo,Zhang Chenhao,Chen Yanyan,Cheng Meidie,Liu Junhong,Sun Jie,Zhi Shuangcheng,Ye Yanxin
A hierarchical
magnetic core–shell gel platform
was developed
for lipase immobilization to achieve enhanced catalytic performance.
The architecture integrates an Fe 3 O 4 magnetic
core, a multiamine-functionalized silica shell for covalent lipase
attachment via glutaraldehyde cross-linking, and a hydrophilic calcium
alginate (CaAlg) gel encapsulation layer. This tripartite design,
denoted as Fe 3 O 4 @SiO 2 L@CaAlg (L:
lipase), achieves an immobilization efficiency of 83.5% under response-surface-optimized
conditions, significantly outperforming its unencapsulated counterpart.
The enhanced performance arises from the synergistic effects of covalent
immobilization and physical encapsulation: the CaAlg gel matrix not
only prevents enzyme leaching but also preserves the essential hydration
layer for lipase interfacial activation, resulting in a reduced apparent
activation energy of 37.8 kJ/mol and superior reusability, with 67.7%
of its initial activity retained after five operational cycles. Notably,
Fe 3 O 4 @SiO 2 L@CaAlg enables a biodiesel
yield of 75.79%, highlighting its potential as an efficient and sustainable
platform for industrial biocatalysis.
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Journal of Future Foods
·
2025
·
IF 7.2
Guihong Yao,Yun Ling,Tong Liu,Shige Xing,Meiyi Yao,Wei Guo,Feng Zhang
Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au) exhibited a high immobilization efficiency (93.9%) and enzyme loading capacity (234.78 µg/mg). The half-life of PS@BC-Au was found to be 362.8 min, which is approximately 1.3 and 2 times longer than that of the free enzyme (185.3 min) and the immobilized enzyme without gold (PS@BC, 275.0 min). Furthermore, PS@BC-Au and PS@BC exhibited up to 63.9% and 47.1% of residual activity after 10 cycles, respectively. Interestingly, the gold nanoparticles in the immobilized system can improve the catalytic efficiency. Consequently, BC, with its porous structure, good stability, large surface area, low cost, and environmental-friendly properties, can serve as a promising support for enzyme immobilized application.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Caixia Zhu,Kaiyuan Wang,Yanqin Lv,Songqin Liu,Yanfei Shen,Yuanjian Zhang
Owing to their capabilities for product separation and controlled substance flow, microfluidic chips (MFC) offer a highly efficient and integrated reaction platform for nanozyme-based cascade catalysis and continuous detection. A critical factor in the design of immobilized cascade nanozyme chips with enhanced catalytic performance is the optimization of the balance between immobilization and active site exposure. Herein, cobalt-doped carbon nanozymes with diverse morphologies (zero-dimensional, one-dimensional, and two-dimensional) were employed as model systems to propose tailored immobilization strategies that balance simplicity, stability, and catalytic efficiency. Mechanism studies showed that the immobilization of nanozymes primarily relies on their close contact with the inner channel walls of the MFC, which results in the physical blockage of active sites on the nanozyme surface. Compared with the compact and spherical morphology of zero-dimensional (0D) nanozymes, the elongated and extended architectures of one-dimensional (1D) and two-dimensional (2D) nanozymes exhibit cross-linked and rich wrinkle structures, which facilitate effective immobilization while minimizing the loss of active sites. Consequently, incorporation of a binder─particularly for 0D nanozymes─was necessary, as it prevents deep embedding into the PDMS substrate and allows greater exposure of catalytically active surfaces. As a proof of concept, a microfluidic biosensor for dopamine detection was developed, demonstrating markedly enhanced stability and sensitivity following optimization of the immobilization interfaces. This work provides guidance for the selection of immobilization methods of different nanozymes in MFCs, thereby improving the practicability of nanozyme-modified MFC systems.
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CHEMOSPHERE
·
2023
·
IF 8.8
Qianting Xu,Fengping Zhou,Qingxin Yu,Ye Xiao,Xinyi Jiang,Weihua Zhang,Rongliang Qiu
In the Pearl River Delta of China, many sites are likely contaminated with aniline in the soil and arsenic (As) in the groundwater because of a high As background level and the prevailing printing and dyeing industry. This study is to explore the remediation performance of thermally activated persulfate oxidation for the sites with these two contaminants, aniline and As. The As influence on the aniline degradation and vice versa are also systematically investigated. When the molar ratio of aniline to persulfate is 1: 4.65, over 85% of aniline can be degraded at 40 °C in 24 h, and 100 μg L −1 As(III) in solution can be completely adsorbed by the soil. A higher pH favored the aniline degradation but disfavored the As(III) oxidation. Due to the strong buffer capacity of the soil, aniline in the soil could be more quickly degraded than those in the solution. The As(III), however, seem more easily oxidized in the absence of soil. The coexisting Fe 2+ can substantially improve As(III) oxidation and immobilization, although the dilute Fe 2+ solution may suppress the aniline degradation. The presence of aniline severely inhibited the As(III) oxidation and adsorption, likely due to the competition for the generated free radicals and the adsorption sites on the soils. In contrast, the existing As(III) has a slight effect on aniline degradation. These findings are believed to provide the theoretical basis for the remediation of aniline-arsenic contaminated sites.
研究领域:
环境科学
·
污染修复
·
化学工程
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Xing Ni,Luyi Chen,Xue Zhang,Jiaojiao Xia,Qiongya Lu,Yani Zhao,Bin Zou
In this study, alkali lignin (AL), a low-cost and renewable biopolymer, was innovatively selected as the precursor. Modified lignin, namely aminated lignin (AAL), sulfomethylated lignin (SML) and phosphorylated lignin (PAL), were synthesized and combined with nano-MgO to serve as a template. Subsequent carbonization of these mixtures produced mesoporous carbon (MC) carriers denoted as AL-MC, AAL-MC, SML-MC and PAL-MC. Rhizomucor miehei lipase (RML) immobilization onto these carriers was effectively achieved through physical adsorption under mild conditions. Through optimization of the preparation parameters, high-performance immobilized enzymes (IM-AL-MC, IM-AAL-MC, IM-SML-MC, IM-PAL-MC) were developed. Notably, IM-AAL-MC exhibited superior enzyme activity (0.037 U/mg immobilized lipase), loading capacity (0.145 mg protein/mg immobilized lipase) and immobilization efficiency (84.5 %). The catalytic efficiency and stability of IM-AAL-MC were comprehensively examined. Characterization of the carriers and immobilized enzymes was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Brunauer-Emmett-Teller surface area analysis (BET) and transmission electron microscopy (TEM). Results indicated that IM-AAL-MC displayed significantly improved stability compared to free lipase. Remarkably, IM-AAL-MC retained 99.49 % activity in n-hexane, far exceeding the free lipase (43.53 %). Additionally, IM-AAL-MC preserved 86.2 % of its relative activity after six consecutive reuse cycles.
研究领域:
生物技术
·
酶工程
·
材料科学
·
绿色化学
·
纳米技术
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Xin Wang,Shengyan Pu,Jiaqi Ding,Jing Chen,Peng Liao,Delai Zhong,Daniel C. W. Tsang,John C. Crittenden,Linling Wang
Clay minerals are ubiquitous in subsurface environments and have long been recognized as having a limited or negligible impact on the fate of arsenic (As) due to their negatively charged surfaces. Here, we demonstrate the significant role of kaolinite (Kln), a pervasive clay mineral, in enhancing As(V) immobilization during ferrous iron (Fe(II)) oxidation at near-neutral pH. Our results showed that Fe(II) oxidation alone was not capable of immobilizing As(V) at relatively low Fe/As molar ratios (≤2) due to the generation of Fe(III)–As(V) nanocolloids that could still migrate easily as truly dissolved As did. In the presence of kaolinite, dissolved As(V) was significantly immobilized on the kaolinite surfaces via forming Kln–Fe(III)–As(V) ternary precipitates, which had large sizes (at micrometer levels) to reduce the As mobility. The kaolinite-induced heterogeneous pathways for As(V) immobilization involved Fe(II) adsorption, heterogeneous oxidation of adsorbed Fe(II), and finally heterogeneous nucleation/precipitation of Fe(III)–As(V) phases on the edge surfaces of kaolinite. The surface precipitates were mixtures of amorphous basic Fe(III)–arsenate and As-rich hydrous ferric oxide. Our findings provide new insights into the role of clay minerals in As transformation, which is significant for the fate of As in natural and engineered systems.
研究领域:
环境科学
·
土壤化学
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Science Bulletin
·
2025
·
IF 21.1
Yulin Wang,Yangzezhi Zheng,Weihuan Li,Shuai Xiao,Shengjun Chen,Jiarui Xing,Chenchen Xiong,Yang Zhou,Wei Zhang,Takehiko Hihara,Nosipho Moloto,Changwen Miao
Buildings and infrastructure significantly contribute to global energy consumption and CO 2 emissions. Transforming cement, the most widely used construction material, into a functional medium for heat harvesting presents a promising avenue to offset the energy demands of buildings. The disparity in diffusion rate between cations and anions within cement pore solution due to variations in interactions with pore walls, endows cement with inherent ionic thermoelectric properties. However, the isolation of pores by the dense cement matrix hinders the rapid transportation of ions with superior diffusion rates, impeding the enhancement of mobility difference between ions and limiting the enhancement of Seebeck coefficient. Inspired by the stem structure of plants, we present a cement-polyvinyl alcohol (PVA) composite (CPC) featuring aligned cement and PVA hydrogel layers. While PVA hydrogel layers provide ion diffusion highways for OH − ions, cement-PVA interfaces establish strong coordination bonds with Ca 2+ ions and weaker interactions with OH − ions, enabling selective immobilization, which amplifies the diffusion rate disparity between Ca 2+ and OH − . The CPC’s multilayer structure yields abundant interfaces, providing ample interaction sites that maximize the contribution of cement ions to thermoelectric performance. The as-prepared composite achieves an impressive Seebeck coefficient of −40.5 mV/K and a figure of merit ( ZT ) of 6.6×10 −2 . Due to the engineered multilayer structure, the CPC also demonstrates superior mechanical strength and intrinsic energy storage potential, which has been assembled into a self-powered architecture. The biomimetic structure and interfacial selective immobilization mechanism may pave the way for the design and fabrication of high-performance ionic thermoelectric materials.
研究领域:
材料科学
·
能源工程
·
建筑技术
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fangrui Yu,Na Li,Lianmei Chen,Yuying Zhang,Xueru Sheng,Bing Wang,Jian Zhang,Qingwei Ping,Hongbin Li
In this paper, an effective strategy to improve the stability and recovery convenience of immobilized laccase by preparing magnetic particles from cheap filter paper fibers was studied, that is, MC@Fe3O4 microparticles were prepared by simple cellulose regeneration and carboxyl group functionalization, and then immobilized laccase under mild conditions. The Box-Behnken design (BBD) was used to optimize the enzyme concentration, pH value and contact time affecting the immobilization yield. The results showed that the concentration of laccase and pH value had important effects on the immobilization yield. The thermal stability, pH stability and storage stability of immobilized laccase were higher than those of free enzyme. Most importantly, the immobilized laccase had a good degradation capacity for bisphenol A (BPA), with a degradation rate of 75.65 % in the first cycle, and the BPA removal rate remained above 35 % even after 8 consecutive reuses. This study developed a new magnetic cellulose material to immobilize laccase, and the immobilized laccase had great potential in BPA wastewater treatment.
研究领域:
生物化学
·
环境科学
·
材料科学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yitong Wang,Huazong Liu,Yan Chu,Jiyu Yang,Ganlu Li,Kequan Chen,Hui Li
Protein aggregates are extremely useful in fields such as bioseparation, biocatalysis, and bioadhesives. The development and use of protein aggregates, however, are in their infancy. In this study, emerging protein aggregates were developed through protein self-assembly using polar aprotic solvents coupled with halides. The polar aprotic solvent molecules, including dimethyl sulfoxide (DMSO), were inserted into the hydration shell of proteins such as ovalbumin (OVA), bovine serum albumin, and whey protein to partially exchange water molecules via van der Waals and electrostatic interactions to alter the surface polarity of the protein. Then, the halide ions were allowed to bind to proteins via electrostatic interactions and cause the self-assembly of proteins into aggregates. Under optimal conditions, the yield of OVA-DMSO protein aggregates reached 91.2 ± 1.52 %. OVA-DMSO-EDC/NHS-CA showed excellent enzymatic properties, and the production of calcium carbonate reached 7.1 ± 0.4 mg at 37 °C for 10 min. Additionally, other enzymes, such as L-TA, had high activity after being fixed with OVA-DMSO. These innovative protein aggregates are highly valuable for developing biomaterials.
研究领域:
生物化学
·
生物材料科学
·
酶工程
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DESALINATION
·
2025
·
IF 9.8
Bin Liu,Junlong Cai,Xin Mao,Fazhi Xie,Xuewu Zhu
The efficient reduction and immobilization of highly toxic and migratory Cr(VI) present significant challenges in chromium-containing wastewater. This research demonstrates that the Fe(II)-coupled micelle-enhanced nanofiltration (MENF) system facilitated the rapid conversion and removal of Cr(VI). The co-complexation of cetyltrimethylammonium chloride (CTAC) and Fe(II) improved the molecular-level coordination and reduction capacity of Cr(VI), leading to its effective transformation to Cr(III) and a 41 % removal rate of Cr. The interaction between CTAC, Cr, and Fe generated larger aggregates, such as CTAC-Cr, (Fe x , Cr 1 − x )(OH) 3 , and CTAC-Cr-Fe, which enhanced Cr immobilization. Moreover, during the Fe(II)-coupled MENF process, a large amount of CTAC-Cr(III)-Fe(II, III) precipitates were removed during the complexation phase, while a small portion of the complexes adhered to the membrane surface, forming a porous cake layer. Due to the increased hydrodynamic diameter of the aforementioned Cr complexes, they could be easily retained by the nanofiltration membrane. Even under interference from high concentrations of anions and humic acids, the Fe(II)-coupled MENF system could still completely remove Cr(VI). This investigation contributes to a holistic understanding of the utilization of Fe(II)-coupled MENF systems and proposes effective sustainable strategies for Cr(VI) removal.
研究领域:
环境科学
·
化学工程
·
水处理技术
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