Nanomaterials
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2016
·Vol. 6
·期 10
·178
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IF 2.69
Yuehua Zhang,Wu Lei,Yujuan Xu,Xifeng Xia,Qingli Hao
A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-modified GCE (PLL/GCE), GO-modified GCE and bare GCE, the PLL/GO/GCE exhibited an increase in the anodic potential difference and a remarkable enhancement in the current responses for both UA and DA. For the simultaneous detection of DA and UA, the detection limits of 0.021 and 0.074 μM were obtained, while 0.031 and 0.018 μM were obtained as the detection limits for the selective detection of UA and DA, using DPV in the linear concentration ranges of 0.5 to 20.0 and 0.5 to 35 μM, respectively. In addition, the PLL/GO/GCE demonstrated good reproducibility, long-term stability, excellent selectivity and negligible interference of ascorbic acid (AA). The proposed modified electrode was successfully implemented in the simultaneous detection of DA and UA in human blood serum, urine and dopamine hydrochloride injection with satisfactory results.
研究领域:
分析化学
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生物医学工程
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电化学传感器
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RSC Advances
·
2015
·Vol. 5
·期 19
·14138-14146
·
IF 3.84
Jiashan Zhou,Yan Li,Haiqing Dong,Hua Yuan,Tianbin Ren,Yongyong Li
Polymeric catiomers, which can mimic viruses for gene packing and delivery, have received considerable attention as nonviral vectors for gene therapy. Inspired by the critically important role of the sequence of structural units in various biomolecules, including DNA and protein, in this study, we intend to investigate how the monomer sequence of a polymeric catiomer affects its gene packing capacity and delivery efficiency. The well-documented poly(histidine-co-lysine) was chosen as the scaffold for gene carrier. Four reducible polycations (RPCs) based on sequence-regulated peptides monomers were synthesized. Chemical parameters (namely, composition and molecular weight) of four RPCs were controlled at comparable level except for the monomer sequence. All of the RPCs exhibited low cytotoxicity and effective DNA binding ability. However, these RPCs displayed distinct diversity from each other, especially in their ability of binding to DNA, buffering capacity and transfection efficiency. Using 293T cell as the mode, we found that the regulation of the monomer sequence of polycations could significantly affect their properties for gene delivery, with differences of 100 fold. The sequence effect might be correlated with different chain folding as well as physiochemical properties of RPCs/pDNA complexes, providing new insight for designing gene vector with promising prospects in gene therapy.
研究领域:
生物医学工程
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分子生物学
·
基因治疗
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高影响因子
FLUID PHASE EQUILIBRIA
·
2015
·
IF 2.2
Shufeng Shen,Ya-nan Yang,Yong Wang,Shaofeng Ren,Jiangze Han,Aibing Chen
Amino acid salts have potential use as alternative absorbents to alkanolamines for post combustion CO 2 capture, because of their negligible volatility, resistance to oxidative degradation and fast absorption rates. In this work, the density and viscosity of CO 2 -free and CO 2 -loaded aqueous solutions of potassium lysinate (LysK) and potassium prolinate (ProK) were measured at temperatures from (298.15 to 348.15) K and for their concentrations from (0.2 to 3.0) mol L −1 . The data were correlated accurately ( R 2 ≥ 0.99) as a function of temperature, concentration and CO 2 loading by nonlinear regression analysis. The solubility of CO 2 in 2.5 mol L −1 amino acid salt solutions was also determined in vapor–liquid equilibrium vessels at temperatures (313 and 333) K and CO 2 partial pressures relevant to flue gas conditions. It was found that, the CO 2 absorption capacity of LysK is higher than that of industrially used monoethanolamine (MEA) and the CO 2 loadings vary as LysK > ProK > MEA at given CO 2 partial pressures. Moreover, LysK may offer higher cyclic loading than MEA for CO 2 capture.
研究领域:
化学工程
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环境科学
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REGULATORY TOXICOLOGY AND PHARMACOLOGY
·
2016
·
IF 2.227
Xin Liu,Liangqing Zheng,Rong Zhang,Gang Liu,Shensheng Xiao,Xiaoting Qiao,Yongning Wu,Zhiyong Gong
Nε-(carboxymethyl)lysine (CML) as a novel potential noxious compound in various food products has aroused extensive concern in recent years. This study aimed to investigate the oral acute and subacute toxicity of CML in mice as per OECD 420 and 407 guidelines. Acute administration of 2000 and 5000 mg/kg CML did not induce any mortality within 14 days, nevertheless some toxicological symptoms and histopathological changes were observed. The estimated LD 50 of CML was >5000 mg/kg. In subacute toxicity test , CML was dosed at 200, 500 and 1000 mg/kg in both genders for 28 days. The body weights reduced which was accompanied with the decrease of food consumptions. Hematology parameters viz. RBC , HGB and MCH showed minor alteration but these were still within normal range. Biochemical analysis of hepatic and renal function markers showed significant elevating in AST , ALT , Cr and BUN etc. Histopathological alterations were observed in lung, liver, kidney and spleen. Subacute toxicity of CML involved oxidative stress caused by reducing antioxidant enzyme (SOD and GSH-Px) activities, and significantly increasing lipid peroxide (MDA) level. In conclusion, CML was unlikely to present an acute hazard, but repeated administration could produce deleterious effects on mice especially inducing liver and kidney damage through oxidative stress.
研究领域:
毒理学
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食品科学
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药理学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 11
·1893-1905
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IF 5.047
Ihsan Ullah,Jing Zhao,Shah Rukh,Khan Muhammad,Jintang Guo,Xiang-kui Ren,Shihai Xia,Wencheng Zhang,Yakai Feng
Gene therapy is concerned with the transfer of complement genes to functionally defective cells in a safe and directed manner for the treatment of the most challenging diseases. But safety issues and low transfection efficiency of the gene vectors are the major challenges, which need to be overcome. Recently, redox-responsive bioreducible polymers containing disulfide linkages have been considered as efficient gene vectors, owing to the selective degradation of the disulfide bond in the reducing environment of the cells. This enables spatiotemporal release of pDNA with no or minimum toxicity. Herein, we reported a bioreducible poly(ethyleneglycol)-b-poly(disulfide-L-lysine) cationic polymer (denoted as PEG–SSL) via a Michael addition reaction of poly(ethyleneglycol)tetraacrylate PEG(Ac)4 and the terminal amine group of poly(disulfide-L-lysine). PEG–SSL efficiently condensed the plasmid ZNF580 gene (pZNF580) forming nano-sized polyplexes (155 ± 4 to 285 ± 3 nm) with zeta potentials of 1.9 ± 0.1 to 26.7 ± 0.4 mV. PEG–SSL successfully retarded pZNF580 at a small polymer/pDNA weight ratio of 10/1 and higher. When exposed to a reducing environment of 5 mM DTT, it rapidly released genes even at higher weight ratios of the PEG–SSL polymer in the PEG–SSL/pDNA complexes. The PEG–SSL/pZNF580 complexes exhibited good stability when exposed to DNase I and efficiently protected pDNA from degradation. In vitro transfection and cytotoxicity were investigated in EA.hy926 cells. The results showed that PEG–SSL successfully delivered pZNF580 into the cells with less cytotoxicity compared to PEI25kDa. The flow cytometry and confocal scanning laser microscopy results indicated that PEG–SSL polyplexes exhibited good cellular uptake and nuclear co-localization rates. All these results implied that PEG–SSL had the potential as a non-viral vector for gene transfection.
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JOURNAL OF MEDICINAL CHEMISTRY
·
2016
·
IF 5.589
Xiaoli Liu,Rui Cao,Sha Wang,Junli Jia,Hao Fei
Cationic amphipathic peptides (CAPs) are known to be able to cause membrane destabilization and induce cell death, yet how the hydrophobicity, amphipathicity, and lysine (K)/arginine (R) composition synergistically affect the peptide activity remains incompletely understood. Here, we designed a panel of peptides based on the well-known anticancer peptide KLA. Increasing hydrophobicity enhanced the cytotoxicities of both the K- and R-rich peptides. Peptides with an intact amphipathic helical interface can cause instant cell death through a membrane lysis mechanism. Interestingly, rearranging the residue positions to minimize amphipathicity caused a great decrease of cytotoxicity to the K-rich peptides but not to the R-rich peptides. The amphipathicity-minimized R-rich peptide 6 (RL2) (RLLRLLRLRRLLRL-NH2) penetrated the cell membrane and induced caspase-3-dependent apoptotic cell death. We found that the modulation of hydrophobicity, amphipathicity, and K/R residues leads to distinct mechanisms of action of cationic hydrophobic peptides. Amphipathicity-reduced, arginine-rich cationic hydrophobic peptides (CHPs) may represent a new class of peptide therapeutics.
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PubMed
高影响因子
Food and Bioprocess Technology
·
2023
·
IF 5.6
Lin Junxin,Sun Peizi,Zhao Yanfen,Du Xiaoping,Ren Xiang,Man Hao,Li Dongmei
Heat treatment reduces the quality of Antarctic krill ( Euphausia superba ) meat, thus greatly limiting its industrial application. It was found that L-Lys immersion pretreatment can effectively improve the quality of heat-treated Antarctic krill meat; the underlying mechanism is unclear. Therefore, this study aimed to investigate the effect of L-Lys concentrations (0, 25, 50, 100, and 200 mM) on the aggregation behavior and structure of Antarctic krill myofibrillar protein solution before and after heat treatment. Compared with the untreated group, L-Lys decreased the surface hydrophobicity and particle size of the heat-treated Antarctic krill protein by 2.38 times and 18.27 times while increasing the solubility by 3.59 times. Furthermore, L-Lys intervention inhibited the formation of disulfide bonds in myofibrillar protein of the heat-treated Antarctic krill, enhanced the intermolecular hydrogen bonding force, improved the orderliness of the secondary structure, and “exposed” the tyrosine residues of the protein molecule. As a result, the polarity of the microenvironment was enhanced while the tertiary structure of the protein was altered, thus inhibiting thermal aggregation. This study reveals the mechanism of L-Lys inhibition of thermal aggregation behavior of Antarctic krill myofibrillar protein. Our results provide insights into the development and utilization of Antarctic krill protein in the food industry.
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ACS Food Science & Technology
·
2023
·
IF 2.3
Yi Wu,Yongtai Wu,Zhenhui Zhang,Jiaxian Cai,Minhua Zong,Lin Li,Xia Zhang,Hong Wu,Bing Li
Advanced glycation end products (AGEs) are widely found in processed foods; their free fractions could significantly affect cellular glucose and lipid metabolism, but this mechanism remains poorly understood. We selected Nε-(carboxymethyl)lysine (CML) as a representative free AGE to study the biochemical processes involved in glucose metabolism, lipid synthesis, and catabolism in human LO2 cells. A combination of biochemical, metabolic flux, and comparative experiments revealed that the mechanism of CML intervention in glucose metabolism mainly involves promoting the dephosphorylation of glucose 6-phosphate and inhibiting the conversion of cis-aconitate to isocitrate in the Krebs cycle. Regarding lipid metabolism, CML combined with high-glucose stimulation synergistically promoted de novo lipogenesis, and CML also promoted fatty acid β-oxidation. These results provide constructive insights into the mechanisms and targets of the imbalance in cellular glucose and lipid metabolism induced by dietary AGEs.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 31
·期 4
·1553-1567
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IF 4.705
Liu Muhua,Zhang Wen,Zhang Panpan,Yuan Ling,Li Hao,Zhan Jinjie,Zhao Wenjun,Mei Xi
Hyperbilirubinemia is one of most severe clinical diseases, which is caused by the accumulation of unconjugated bilirubin. Electrospun nanofiber membranes used as highly efficient bilirubin adsorbents have been applied to remove the extra bilirubin in hemoperfusion for their high surface area and easy functionalized properties. In this work, Lysine (Lys) grafted polyacrylonitrile (PAN) electrospun nanofiber membranes doped with organic Hectorite (OHec) (Lys-HPAN@OHec) have been fabricated via a series of modified process, including pore forming, alkaline hydrolysis and grafting reaction. The obtained Lys-HPAN@OHec nanofiber membranes have been analyzed in detail and investigated the adsorption capacity for bilirubin. Compared with original PNA membranes, Lys-HPAN@OHec nanofiber membranes show an excellent bilirubin adsorption capacity and more stable rejection rate of bovine serum albumin (BSA). The maximum adsorption capacity of Lys-HPAN@OHec membranes for bilirubin is 64 mg/g, the adsorption process of Lys-HPAN@OHec membranes matched the Langmuir model well. In addition, dynamic adsorption reveals that the adsorption equilibrium time of Lys-HPAN@OHec membranes is about 2 h. Significantly, Lys-HPAN@OHec membranes have excellent biocompatibility and hemocompatibility. This study demonstrates that the novel Lys-HPAN@OHec membranes may provide a new way to treat hyperbilirubinemia.
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MOLECULES
·
2023
·Vol. 28
·期 3
·1391
·
IF 4.6
Yunyi Liang,Yonghong Luo,Yang Wang,Tianyang Fei,Lili Dai,Daihui Zhang,Hongzhi Ma,Liping Cai,Changlei Xia
Soy protein isolate (SPI) is an attractive natural material for preparing wood adhesives that has found broad application. However, poor mechanical properties and unfavorable water resistance of wood composites with SPI adhesive bonds limit its more extensive utilization. The combination of lysine (Lys) with a small molecular structure as a curing agent for modified soy-based wood adhesive allows Lys to penetrate wood pores easily and can result in better mechanical strength of soy protein-based composites, leading to the formation of strong chemical bonds between the amino acid and wood interface. Scanning electron microscopy (SEM) results showed that the degree of penetration of the S/G/L-9% adhesive into the wood was significantly increased, the voids, such as ducts of wood at the bonding interface, were filled, and the interfacial bonding ability of the plywood was enhanced. Compared with the pure SPI adhesive, the corresponding wood breakage rate was boosted to 84%. The wet shear strength of the modified SPI adhesive was 0.64 MPa. When Lys and glycerol epoxy resin (GER) were added, the wet shear strength of plywood prepared by the S/G/L-9% adhesive reached 1.22 MPa, which increased by 29.8% compared with only GER (0.94 MPa). Furthermore, the resultant SPI adhesive displayed excellent thermostability. Water resistance of S/G/L-9% adhesive was further enhanced with respect to pure SPI and S/GER adhesives through curing with 9% Lys. In addition, this work provides a new and feasible strategy for the development and application of manufacturing low-cost, and renewable biobased adhesives with excellent mechanical properties, a promising alternative to traditional formaldehyde-free adhesives in the wood industry.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Qian Han,Kangkang He,Ruisheng Han,Jianda Zhang
Electropolymerization of L-lysine incorporating multiwall carbon nanotubes (MWCNTs) dispersed in β -cyclodextrin prepolymer (CDP) solution was constructed a very efficient sensor. Scanning electron microscopy was employed to characterize the surface morphology of the modified electrode. The proposed sensor excellently enhanced the selectivity in the simultaneous determination of HQ and CC by differential pulse voltammetry and cyclic voltammetry. Comparing with bare GCE, MWCNTs/GCE, CDP-MWCNTs/GCE, the introduction of L-lysine polymer film effectively declined the shoulder peak of buffer soultion which seriously interfered the dection of hydroquinone (HQ) and catechol (CC) isomers. The PLL/CDP-MWCNTs/GCE displayed good discrimination ability and high sensitivity toward HQ and CC, peak-to-peak separation about 105 mV were obtained. The linear response range for hydroquinone and catechol were 0.1 μM – 100 μM. The detection limits of HQ and CC were 0.044 μM, 0.058 μM, respectively.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Xianguang Zeng,Lin Zhu,Xingwen Zheng
The inhibitive effect of N-lauroyl L-lysine on the corrosion of mild steel in 0.5 M H 2 SO 4 solution was investigated by electrochemical technique, weight loss measurement, scanning electron microscope (SEM) and quantum chemical calculation. The results showed that N-lauroyl L-lysine was a moderate cathodic inhibitor for mild steel in 0.5 M H 2 SO 4 solution with its inhibition efficiency increasing with concentration, and decreasing with temperature. The adsorption of N-lauroyl L-lysine on the surface of mild steel obeyed El-Awady thermodynamic-kinetic model, and the thermodynamic and kinetic parameters, as well as quantum chemical parameters, were determined to discuss the mechanism of inhibition of N-lauroyl L-lysine on mild steel surface.
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高影响因子
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