Molecular Catalysis
·
2020
·
IF 3.687
Jingyi Yang,Yanping Fan,Zhong-Li Li,Zhikun Peng,Jing-He Yang,Baozhong Liu,Zhongyi Liu
Herein, we reported an effective method to synthesis a series of supported bimetallic Pd-M/SiO 2 (M = Pt, Ni, Cu, Co) catalysts with highly exposed and well-alloyed nanoparticles via simultaneous strong electrostatic adsorption (co-SEA), which controlled metal precursors strongly anchored onto the oppositely charged support (SiO 2 ) by changing the pH relative to the surface point of zero charge (PZC). The toluene and benzene hydrogenation were used as probe reactions to evaluate the catalytic hydrogenation activity performance of the Pd-M/SiO 2 catalysts. Compared to the catalysts synthesized by the common technique simultaneous dry impregnation (co-DI), the co-SEA Pd-Ni/SiO 2 catalyst exhibited the highest turnover frequencies (TOFs) of 3.7 s −1 for benzene hydrogenation and 3.2 s −1 for toluene hydrogenation. This was attributed to the large amount of electron-deficient Pdδ + sites on the surface of SEA Pd-M/SiO 2 that easily for electron-rich benzene and toluene to adsorb. Besides, the co-SEA catalysts produced well stability and reusability without significant decrease in the catalytic activity after six runs.
研究领域:
催化
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材料科学
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化学工程
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 43
·26625-26632
·
IF 3.108
Jieqiong Cao,Qiong Wu,Wenjie Zheng,Li Li,Wenjie Mei
A series of polypyridyl ruthenium(II) complexes coordinated by phenanthroimidazole derivatives [Ru(phen)2(R)](ClO4)2 (where 1 R = IP, 2 R = PIP, 3 R = p-HPIP, 4 R = p-OCH3PIP) was synthesized with an average yield of >85% under microwave irradiation at 140 °C for 30 min. The inhibitory effect of these complexes against various tumour cells were evaluated by MTT assay, and the results showed that these polypyridyl ruthenium(II) complexes exhibited acceptable inhibition against different tumour cells, especially 4, with an IC50 of 18.4 μM for Hela cells. The results showed that 4 inhibited the growth of cervical cancer Hela cells by inducing G2/M phase arrest, which was followed by slight apoptosis. Further studies showed that 4 displayed better inhibition against the invasion and metastasis of Hela cells than NAMI-A. Studies on the in vivo distribution and metabolism indicated that 4 was rapidly distributed in the entire body, absorbed by the tumour tissue and had only a small accumulation of toxicity in the body. These results demonstrated that this type of ruthenium(II) complex can block the growth of Hela cells and inhibit their migration and invasion through G2/M phase arrest, which suggests the complex could act as a potential tumour-targeting inhibitor in future clinical applications.
研究领域:
药理学
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肿瘤学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Lei Xie,Yiping Zhang,Ling Chen,Tianxing Wang,Shuo Zhang,Xiaoxi Li
Characterizing the structural changes of cell-targeting delivery carriers in gastrointestinal tract (GIT) is crucial for understanding their effectiveness in cell targeting and transport. Herein, RGD peptide-grafted carboxymethyl starch (CMS) and cationic quaternary ammonium starch (QAS) were utilized to fabricate quintet-layered nanocapsules loaded with ovalbumin (OVA). The aim was to improve delivery and transportation efficiency, specifically targeting M cells. The research analyzed the impact of pH and enzyme variations in GIT on the structure of nanocapsules, interactions between carriers and the release behavior of OVA. Results showed that the size of nanocapsules increased from 229.2 to 479.8 nm and the zeta potential decreased from −1.08 to −33.33 mV during oral delivery. This was evident in TEM images, showing a more relaxed core-shell structure. Isothermal titration calorimetry and molecular dynamic simulation indicated that pH changes primarily affected the electrostatic interaction between carriers. Increasing pH led to reduced affinity constants, and around 84.42 % of OVA was successfully delivered to M cells. Moreover, the transport efficiency of nanocapsules to M cells was five times greater than that of Caco-2 cells. This suggests the feasibility of developing a nanocapsules delivery system capable of adapting to pH changes in GIT by regulating electrostatic interactions between carriers.
研究领域:
食品科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
·
IF 10.7
Kaiping Wang,Yuxuan Liu,Zeming Zhang,Ziming Zheng,Wenqi Tang,Wangtianzi Teng,Xu Mu,Jinglin Wang,Yu Zhang
Lentinan (LNT), a natural polysaccharide, has been reported to exhibit immunomodulatory effects in the intestine after oral administration. Herein, we aimed to investigate the lymphatic transport of LNT in Peyer's patches (PPs) by traceable fluorescent labeling and to explore whether/how LNT contacts related immune cells. Near-infrared imaging confirmed the absorption of LNT in the small intestinal segment and its accumulation within PPs after oral administration. Subsequently, tissue imaging confirmed that M cells are the main cells responsible for transporting LNT to PPs, and an M cell model was established to explore the involvement of Dectin-1 in the absorption process. Systematic in vitro and in vivo studies revealed that the Dectin-1 further mediates the uptake of LNT by mononuclear phagocytes in PPs. Moreover, LNT can promote the proliferation and differentiation of mononuclear phagocytes, thereby activating immune responses. In summary, this study elucidates the pharmacokinetic mechanisms by which LNT exerts oral immunomodulatory effects, providing a theoretical basis for the development and application of other polysaccharides.
研究领域:
免疫学
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药理学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Journal of Rubber Research
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2019
·Vol. 23
·期 1
·41-46
·
IF 0.281
Liao Xiao-Xue,Wei Yan-Chan,Zhang Hui-Feng,Zheng Ting-Ting,Luo Ming-Chao,Liao Shuangquan
Waste rubber with three-dimensional structure is insoluble and non-melting, which creates recycling problem. The technique called “high-pressure high-temperature sintering” (HPHTS) can convert vulcanized rubber powder into usable rubber products. However, reversion occurs when vulcanizates are exposed to a prolonged treatment at high temperature. This reversion results in the decrease of mechanical properties of sintered waste rubber powder (WRP) samples. To address this problem, a method of enhancing the mechanical properties of sintered WRP by incorporating different content of maleic anhydride (MA) and N , N ′- m -phenylene bismaleimide (BMI) is discussed in this paper. The results show that BMI and MA can slow down the formation of the conjugated double bonds. Through Diels–Alder reaction, BMI inhibits conjugated double bonds formation and improves the adhesion between particles and particles, which is beneficial to improvement of final properties. Therefore, BMI improves the mechanical properties of sintered WRP greatly.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2018
·Vol. 93
·期 9
·2552-2563
·
IF 2.587
Yi Yang,Huiping Zhang,Ying Yan
BACKGROUND A novel iron-loaded microfibrous entrapped carbon-nanotube catalyst (Fe 2 O 3 -MF-CNT) was synthesized by wet lay-up papermaking, sintering and metal organic chemical vapor deposition (MOCVD) processes. The catalysts were characterized by N 2 adsorption–desorption isotherm, mercury porosimetry, scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS) and NH 3 temperature program desorption (NH 3 -TPD), and the catalytic performance was tested by catalytic wet peroxide oxidation (CWPO) of m-cresol in a fixed-bed reactor. Finally, the probable multistep reaction pathway for CWPO degradation of m-cresol over Fe 2 O 3 -MF-CNT catalyst in a fixed-bed reactor is presented. RESULTS Characterization results conclude that the prepared catalysts are acid catalysts containing macropores and mesopores. Active components Fe 2 O 3 are evenly loaded on the support by the MOCVD method. CWPO results show that higher temperature and higher space time promote the catalyst performance. Meanwhile, the Fe 2 O 3 -MF-CNT catalyst shows perfect reusability with nearly no iron leaching concentration and high m-cresol conversion (above 95.0%) after four successive runs (24 h). Two possible pathways during degradation of m-cresol are presented. The hydroxyl radicals turn m-cresols into aromatic by-products such as p-toluquinone, 4-methylpyrocatechol and methylhydroquinone, followed by evolution to organic acids, and finally to carbon dioxide and water. During the reaction, more m-cresols are degraded through the pathway in which m-cresols are first converted into 4-methylpyrocatechol. CONCLUSION The novel Fe 2 O 3 -MF-CNT catalyst presented herein owns the advantages of both CNTs and stainless steel fibers, and it shows promising performance in CWPO of m-cresol. © 2018 Society of Chemical Industry
研究领域:
环境科学
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催化化学
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DOI
高影响因子
PHYTOMEDICINE
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2019
·
IF 4.18
Jun-Shan Liu,Chu-Ying Huo,Hui-Hui Cao,Chun-Lin Fan,Jian-Yang Hu,Li-Juan Deng,Zi-Bin Lu,Hua-Yi Yang,Lin-Zhong Yu,Zhi-Xian Mo,Zhi-Ling Yu
Background Hepatocellular carcinoma (HCC) ranks third among the most common causes of cancer-related deaths worldwide. The chemotherapy for HCC is still insufficient, so far. In searching for effective anti-HCC agents from traditional Chinese medicine , we discovered that aloperine (ALO), a quinolizidine alkaloid from Sophora alopecuroides L., exerts anti-HCC activities. However, the effects of ALO on HCC have been rarely studied, and its underlying mechanisms remain unknown. Purpose This study aims to evaluate the anti-HCC activities of ALO and explore its underlying mechanisms. Methods MTT assay and colony formation assay were used to investigate the anti-proliferative effects of ALO on human HCC Hep3B and Huh7 cells. Hoechst 33258 staining was used to observe the morphological changes of cells after ALO treatment . Flow cytometry was used to analyze apoptosis induction, the collapse of the mitochondrial membrane potential and cell cycle distribution. Western blotting was used to examine the expression levels of proteins associated with apoptosis and cell cycle arrest , and key proteins in the PI3K/Akt signaling pathway . Small interfering RNA (siRNA) transfection was used to investigate the role of Akt in ALO-induced apoptosis and cell cycle arrest. Zebrafish tumor model was used to evaluate the anti-HCC effects of ALO in vivo. Results ALO inhibited the proliferation of Hep3B and Huh7 cells. ALO induced apoptosis in HCC cells, which was accompanied by the loss of mitochondrial potential, the release of cytochrome c into cytosol, as well as the increased cleavages of caspase-9, caspase-3 and PARP . Moreover, ALO induced G2/M cell cycle arrest by downregulating the expression levels of cdc25C, cdc2 and cyclin B1 . In addition, ALO inhibited activation of the PI3K/Akt signaling pathway by decreasing the expression levels of p110α , p85, Akt and p-Akt (Ser473) . Further study showed that inhibition of Akt by siRNA augmented ALO-mediated apoptosis and G2/M cell cycle arrest in HCC cells. Critically, ALO inhibited the growth of Huh7 cells in vivo. Conclusion We first demonstrated that ALO induced apoptosis and G2/M cell cycle arrest in HCC cells through inhibition of the PI3K/Akt signaling pathway. This study provides a rationale for ALO as a potential chemotherapeutic agent for HCC.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2023
·
IF 5.1
Xintong ZHANG,Xiaoxue GAI,Lihua XU,Wenxue MA,Qiaohan LIU,Bendong SHI,Cheng FANG,Jingzeng CAI,Ziwei ZHANG
The aim of this study was to investigate the role of selenoprotein M (SelM) in endoplasmic reticulum stress and apoptosis in nickel-exposed mouse hearts and to explore the detoxifying effects of melatonin. At 21 d after intraperitoneal injection of nickel chloride (NiCl 2 ) and/or melatonin into male wild-type (WT) and SelM knockout (KO) C57BL/6J mice, NiCl 2 was found to induce changes in the microstructure and ultrastructure of the hearts of both WT and SelM KO mice, which were caused by oxidative stress, endoplasmic reticulum stress, and apoptosis, as evidenced by decreases in malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) activity. Changes in the messenger RNA (mRNA) and protein expression of genes related to endoplasmic reticulum stress (activating transcription factor 4 (ATF4), inositol-requiring protein 1 (IRE1), c-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP)) and apoptosis (B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), Caspase-3, Caspase-9, and Caspase-12) were also observed. Notably, the observed damage was worse in SelM KO mice. Furthermore, melatonin alleviated the heart injury caused by NiCl 2 in WT mice but could not exert a good protective effect in the heart of SelM KO mice. Overall, the findings suggested that the antioxidant capacity of SelM, as well as its modulation of endoplasmic reticulum stress and apoptosis, plays important roles in nickel-induced heart injury.
研究领域:
毒理学
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心脏病学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Songze Wang,Xin Zhou,Xin Wang,Shengyu Cheng,Xi-Ling Li,Jun Nan,Jun Zhe Min
Human hair is a non-invasive biological sample that is easy to collect and store and can reflect long-term body health. However, the correlation between DL-amino acids and metabolic diseases in hair samples has not been studied. Therefore, we propose a novel UHPLC-HRMS method for analyzing seven free chiral amino acids (DL-Thr, DL-Glu, DL-Ala, DL-Val, DL-Pro, DL-Leu, and DL-Phe) simultaneously in hair samples by derivatization of chiral probe 4-(N,N-dmethylaminosulfonyl)-2,1,3-benzoxadiazole-trans-2-methyl-L-proline (DBD-M-Pro) labeled with targeted amino functional groups. Gradient elution was carried out using an ACQUITYTM BEH C18 (100×2.1 mm,1.7 μm) column with a mobile phase of 0.15 % formic acid (FA) in 10 mM ammonium acetate (CH 3 -COONH 4 ) and 0.2 % FA in acetonitrile. The labelled DL-amino acid diastereoisomers could be completely separated, with a resolution (Rs) of 1.59–11.44. These amino acids show a strong linear correlation within the range of 3.1–99.2 pmol (R 2 ≥ 0.9990). Intraday and interday precision was 1.87 %–14.87 %. The average recovery was 96.12 %–105.33 %. The limit of detection (LOD) ranged from 0.29 to 2.11 pmol. We then employed the method to determine the concentration of free chiral amino acids in hair samples from 30 healthy volunteers (HVs) and 30 diabetes patients (DPs). Male diabetes patients had significantly higher levels of L-Thr, L-Val, L-Leu ( p < 0.05), and D-Ala ( p < 0.01) in their hair samples than male healthy volunteers and female diabetes patients had significantly higher levels of D-Ala ( p < 0.05) in their hair samples than female healthy volunteers. This is the first study to confirm the feasibility of using free DL-amino acids in human hair as potential biomarkers for diabetes.
研究领域:
分析化学
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代谢组学
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临床化学
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产品关联
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2020
·
IF 6.992
Tengfei Li,Meng Lu,Yuhang Gao,Xiaodong Huang,Guangyang Liu,Donghui Xu
In this study, a novel metal-organic framework (M-ZIF-8@ZIF-67) was successfully prepared using the single layer Fe 3 O 4 -ZIF-8 as magnetic core and wrapped a layer of ZIF-67 outer. This M-ZIF-8@ZIF-67 was employed as an adsorbent for the adsorption and removal of fipronil and its metabolites from environmental water and cucumber samples. The characterization results suggested that M-ZIF-8@ZIF-67 has the double layer structure a polyhedral structure with uniform pores, while ZIF-67 was successfully coated on the surface of Fe 3 O 4 -ZIF-8. The unique structure endowed M-ZIF-8@ZIF-67 a high surface area (219 m 2 /g) and high adsorption capacity for fipronil, fipronil desulfinyl, fipronil sulfide and fipronil sulfone. To our knowledge, this is the first report detailing the adsorption properties of M-ZIF-8@ZIF-67 with double layer structure relating to the adsorption and removal of pesticides. Furthermore, the adsorption model analysis demonstrated that the static adsorption data fitted the Freundlich bimolecular layer adsorption model better than the Langmuir monolayer adsorption model. This study indicates that M-ZIF-8@ZIF-67 has significant potential in the adsorption and removal of fipronil and its metabolites in water and vegetable samples.
研究领域:
环境科学
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材料科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2018
·Vol. 5
·期 3
·
IF 2.504
Yi Yang,Huiping Zhang,Ying Yan
Fe2O3-ZSM-5 catalysts (0.6 wt% Fe load) prepared by metal-organic chemical vapour deposition (MOCVD) method were evaluated in the catalytic wet peroxide oxidation (CWPO) of m-cresol in a batch reactor. The catalysts have a good iron dispersion and small iron crystalline size, and exhibit high stability during reaction. In addition, the kinetics of the reaction were studied and the initial oxidation rate equation was given. Catalysts were first characterized by N2 adsorption–desorption isotherms, scanning electronic microscopy, energy-dispersive spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Results show that extra-framework Fe3+ species (presenting in the form of Fe2O3) are successfully loaded on ZSM-5 supports by MOCVD method. Performances of catalysts were tested and effects of different temperature, stirring rate, catalyst amount on hydrogen peroxide, m-cresol, total organic carbon (TOC) conversion and Fe leaching concentration were studied. Results reveal that catalytic activity increased with higher temperature, faster stirring rate and larger catalyst amount. In all circumstances, m-cresol conversion could reach 99% in 0.5–2.5 h, and the highest TOC removal (80.5%) is obtained after 3 h under conditions of 60°C, 400 r.p.m. and catalyst amount of 2.5 g l−1. The iron-leaching concentrations are less than 1.1 mg l−1 under all conditions. The initial oxidation rate equation −rA0=7.2×1011e−81300/RTCA is obtained for m-cresol degradation with Fe2O3-ZSM-5 catalysts.
研究领域:
环境科学
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2018
·Vol. 135
·期 23
·46354
·
IF 1.901
Bing Chen,Tingting Li,Zheng Zhang,Sui Wang,Zhiyong Guo,Yufang Hu
A promising strategy to design crosslinked photoluminescent hydrogel (PL hydrogel) is to synthesize the covalently bonded polymer chains by thermal polymerization. A proper ratio of methacrylic acid and m -phenylenediamine was used to prepare the PL hydrogel, and doping with graphene oxide and carboxymethyl chitosan improves the structure of the gel. The green and efficient hydrothermal synthesis realized a high polymerization and a short reaction time. Meanwhile, a series of properties were investigated for several combinations of hydrogels. Ultraviolet spectra, fluorescence spectra, and particle size distributions were used to characterize the PL composites. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, rheological studies, and stress–strain tests were conducted to characterize the morphology, structure, and performance of the compound hydrogel. The adsorption properties of the PL hydrogel were characterized in adsorption tests. The results indicated that the PL hydrogel exhibited a favorable luminescence property, a certain degree of mechanical strength, and good adsorption performance. The prepared PL hydrogel has potential applications in adsorption and visual detection. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46354.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）