Immunity Inflammation and Disease
·
2023
·Vol. 11
·期 2
·e730
·
IF 3.2
Min Liu,Shuang Liu,Fajiu Li,Chenghong Li,Shi Chen,Xiaoyan Gao,Xiaojiang Wang
Objective Asthma is a chronic respiratory disease with an increasing incidence every year. microRNAs (miRNAs) have been demonstrated to have implications for asthma. However, limited information is available regarding the effect of miR-124-3p on this disease. Therefore, this study aimed to explore the possible effects of miR-124-3p and S100A4 on inflammation and epithelial–mesenchymal transition (EMT) in asthma using mouse models. Method Ovalbumin was used to induce asthmatic mouse models. Lung injury in mouse models was assessed, and the bronchoalveolar lavage fluid of mice was collected to determine the number of eosinophilic granulocytes and assess inflammation. The expression levels of miR-124-3p, S100A4, E-cadherin, N-cadherin, Snail1, vimentin, and TGF-β1/Smad2 signaling pathway-related proteins were measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. In vitro experiments, cells were transfected with miR-124-3p mimics or inhibitors to test the expression of S100A4 by RT-qPCR and western blot analysis, and the mutual binding of miR-124-3p and S100A4 was validated by dual-luciferase reporter gene assay. Results Overexpression of miR-124-3p or inhibition of S100A4 expression attenuated bronchial mucus secretion and collagenous fibers and suppressed inflammatory cell infiltration. Additionally, upon miR-124-3p overexpression or S100A4 suppression, eosinophilic granulocytes were decreased, interleukin-4 (IL-4) and IL-13 expression levels were reduced in the bronchoalveolar lavage fluid, serum total IgE level was reduced, and the TGF-β1/Smad2 signaling pathway was suppressed. Mechanically, a dual-luciferase reporter gene assay verified the binding relationship between miR-124-3p and S100A4. Conclusion miR-124-3p can negatively target S100A4 to attenuate inflammation in asthmatic mouse models by suppressing the EMT process and the TGF-β/smad2 signaling pathway.
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MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Minting Li,Nengwu Zhu,Wen Zhu,Sihai Zhang,Fei Li,Pingxiao Wu,Xinyu Li
Tetracyclines, cheap and broad-spectrum antibiotics, are required to strictly monitor since they can lead to ecosystem disorders and the flood of resistance genes if overused. Although ovalbumin has been reported to synthesize metal nanoclusters for fluorescence detection, relatively low fluorescence intensity and quantum yield limited their application. In this study, ovalbumin-stabilized gold nanoclusters (OVA-AuNCs) modified by polyethyleneimine (PEI-OVA-AuNCs) with higher fluorescence intensity and quantum yield were successfully synthesized and applied to develop a sensitive fluorescent detection method for tetracycline antibiotics. The fluorescence intensity of the stable PEI-OVA-AuNCs modified with 10 μM PEI was 8.5 times stronger than unmodified OVA-AuNCs. The reason of the fluorescence intensity enhancement could be attributed to etching OVA-AuNCs from large particles to smaller ones by PEI and formation of a protective layer on the outside of the clusters to avoid non-radiative relaxation. The fluorescence quenching was linearly related to tetracycline (TC) in the concentration range 1–30 μM, and the detection limit was 0.563 μM. Meanwhile, the fluorescent probe could detect oxytetracycline and chlortetracycline with the linear correlations higher than 0.99. The quenching mechanism was inner filter effect caused by the overlap of the spectra of PEI-OVA-AuNCs and pollutants, and the aggregation-induced quenching caused by the binding of phenolic hydroxyl and ketone carbonyl groups on the pollutants to the probe as well. The method was successfully applied to the determination of TC in real samples. Therefore, PEI-OVA-AuNCs could be considered as a core material for tetracyclines fluorescence detection.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zuyue Li,Shasha Wang,Mahmoud Abou-Elsoud,Yijie Li,Houchuntai Wang,Meiyu Liu,Wei Hu,Dong Uk Ahn,Xi Huang
NVMEERKIK, a peptide derived from ovalbumin, exhibited remarkable immunomodulatory activity. This study investigated the effect of simulated gastrointestinal digestion on its structure and bioactivity. NVMEERKIK was found to be unstable against gastrointestinal enzymes and completely degraded into NVME, NVMEE, KIK, K, and R. Among these, NVME, which constituted 90.90% of the digest, was synthesized and demonstrated a superior immune-enhancing activity than NVMEERKIK. Specifically, NVME improved phagocytosis, NO production, and TNF-α content in RAW264.7 cells by 1.31–15.86%, 17.17–122.08%, and 0.36–16.76%, respectively. TLR4 inhibition and immunofluorescence assays revealed the strong TLR4 activation and recognition capacities of both peptides. Furthermore, the Western blot results showed that NVMEERKIK and NVME activated the MAPK/NF-κB pathway by upregulating ERK, JNK, p38, and p65, leading to enhanced activation of RAW264.7 cells. The improved immune-enhancing activity of NVMEERKIK after digestion highlighted its potential as an immunomodulatory peptide for functional food applications.
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Journal of Biomedical Nanotechnology
·
2019
·Vol. 15
·期 1
·100-112
·
IF 0
Wei Chang,Dong Xia,Liang Jie,Zhang Yan,Zhu Dunwan,Kong Deling,Lv Feng
The vaccine delivery process plays a significant role for antigen-specific immune responses, which directly determines the effect of immunotherapy. It is crucial to reveal the real-time process of the vaccine release and the status of the implanted carrier in vivo. Herein, a
dual fluorescent vaccine delivery system was monitored with the rhodamine-ovalbumin loaded zinc phthalocyanine conjugated nanoparticles (PHA-OVA NPs) to explore the vaccine delivery process. The PHA-OVA NPs showed a sustained release of ovalbumin in vitro and could significantly enhance
the uptake of antigen by dendritic cells (DCs). The PHA-OVA NPs could also upregulate the levels of costimulatory molecules on the surface of DCs, suggesting an enhanced ability of the antigen delivery system to promote antigen presentation to antigen-special T cells compared to soluble antigen.
The fates of the ovalbumin and the PHA NPs were tracked simultaneously in vivo using the multispectral fluorescence imaging system and the results showed that the antigen was carried to the lymphoid organs by the delivery vehicle. The multispectral fluorescence imaging provided an innovative
method to track the vaccine delivery process simultaneously.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Yi Sun,Bao Chen,Haobo Jin,Sadaqat Ali,Yuanyuan Zhang,Xiaohui Lv,Xiang Fan,Guofeng Jin,Long Sheng
This study investigated the interaction between trans-cinnamaldehyde (TC) and ovalbumin (OVA) at the molecular level and its impact on the gel properties of OVA. Multispectral analysis, molecular docking, and molecular dynamics simulations were employed to elucidate the underlying mechanisms. At room temperature, TC bound to OVA mainly through non-covalent interactions, characterized by static quenching, which consequently altered the secondary structure of OVA. Moderate TC incorporation significantly enhanced the textural properties of thermally induced OVA gels, with hardness, chewiness, and gumminess increased by 39.4%, 28.0%, and 34.9%, respectively (P < 0.05 ). In addition, TC treatment improved the gel's water-holding capacity (by 4.38%), optimized water distribution, and promoted a more uniform microstructure. These improvements were primarily attributed to TC-induced Schiff base formation and non-covalent interactions, which collectively modulated intermolecular forces within OVA. Overall, this study provides novel insights into the use of spice-derived compounds to enhance the structure and functional quality of protein-based foods.
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Food Chemistry-X
·
2026
·
IF 9.2
Zhi-Wei Liu,Xiang-Xiang Xu,Zi-Jing Yin,Waqar Ahmed,Marwa Ezz El-Din Ibrahim,Rana Muhammad Aadil,Hong-Ying Guo
Cold plasma (CP)-induced covalent conjugation between ovalbumin (OVA) and three rosemary-derived phenolic acids (RDPAs), namely chlorogenic acid (CGA), caffeic acid (CA), and rosmarinic acid (RA), was investigated to elucidate how phenolic acid molecular architecture governs the structure, functionality, in vitro digestibility, and allergenicity of OVA. After 45 s of CP treatment (40 kV, 13 kHz), the covalent grafting efficiency of OVA-RDPAs conjugates showed a clear structure dependence, following the order OVA-RA (86.55 ± 2.39 μmol/g) > OVA-CA (79.27 ± 1.62 μmol/g) > OVA-CGA (53.07 ± 2.16 μmol/g). This distinct hierarchy is attributed to RA's dual aromatic rings and four evenly distributed hydroxyl groups, which provide more accessible reactive sites, in contrast to the substantial steric hindrance imposed by CGA's bulky cyclohexane moiety. Multi-spectroscopic and SDS-PAGE analyses revealed that RDPAs grafting induced pronounced conformational unfolding and intermolecular cross-linking in OVA. Consequently, these structure-driven changes translated into remarkably augmented emulsifying and antioxidant properties and lower allergenicity of OVA. Among the conjugates, OVA-RA exhibited superior improvements, achieving 5.1-fold and 14.6-fold increases in DPPH and ABTS radical scavenging capacities, respectively. Notably, RDPAs incorporation effectively masked antigenic epitopes, and OVA-RA exhibited the greatest reduction in IgE-binding capacity (65.81 ± 0.74%). Furthermore, covalent grafting of RDPAs, particularly RA, effectively promoted gastrointestinal digestive trajectories of OVA, and the resulting digests substantially reduced IgE-binding affinity. These findings suggest that CP-mediated covalent RDPAs conjugation is a mild and effective, structure-guided approach that holds potential for the development of egg protein ingredients with enhanced functionality and reduced allergenicity.
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Food Chemistry-X
·
2025
·
IF 8.2
Han Wang,Yanghong Zhao,Lisha Niu,Chi Ren,Yajing Qi,Ying Shu,Kaixuan Zhao,Fengyang Wu,Zhisheng Zhang,Wenhui Qi
Improving the high physical stability of Pickering emulsions, while maintaining their low viscosity, faces enormous challenges. In this study, DAC-OVA conjugates (D-OVA) was prepared using dialdehyde cellulose ovalbumin (OVA) via a Schiff base reaction. Then, the characterization of the D-OVA stabilized Pickering emulsions (DPE) were studied. The results showed that the particle size of DAC was decreased, and the initial morphology disappeared after OVA modification. Compared with OVA-stabilized Pickering emulsion (OPE), the zeta potential of DPE increased significantly at different pH values, especially at pH 4.6 and pH 7.0, which increased about 2.2 and 3.5 times, respectively. Scanning electron microscopy revealed that D-OVA formed a rigid film at the oil-water interface, and DPE droplets exhibited homogeneous dispersion. The rheological results demonstrated that the viscosity of DPE remained consistently low across varying pH and high temperature conditions. Compared with OPE, the freeze-thaw, thermal, and storage stability of DPE have significantly improved.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Fei Lu,Yanqiu Ma,Jingnan Zang,Mingmin Qing,Zihong Ma,Yujie Chi,Yuan Chi
In this study, ovalbumins (OVAs) were glycosylated with fructo-oligosaccharide (FO) at different temperatures (80 °C, 100 °C, 120 °C, and 140 °C) and durations (1 h and 2 h) via wet-heating. The glycosylated OVAs (GOVAs) were characterized by the degree of glycosylation (DG), particle size, zeta potentials, and structural changes. GOVAs-stabilized high-internal-phase emulsions (HIPEs) were then prepared to compare their macro- and microstructure and freeze–thaw stability. The results showed that the DG of GOVAs increased with the increase in glycosylation temperature and the protein structure unfolded with it. Glycosylation decreased the particle size, zeta potential, and α-helical structures and increased the β-sheets and surface hydrophobicity (H 0 ) of GOVAs compared with unmodified OVAs. Moreover, GOVAs-stabilized HIPEs exhibited smaller particle sizes, zeta potentials, agglomeration indexes, oil loss rates, and freezing points and higher viscoelasticity, centrifugal stabilities, flocculation indexes, and freeze–thaw stabilities. Notably, HIPEs prepared by GOVAs (glycosylated higher than 120 °C) showed the least changes in macro- and microscopic appearances after freeze-thawing. These findings will provide a novel method for improving and broadening the functionalities of OVAs and potentially develop HIPEs with enhanced freeze–thaw stabilities.
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MOLECULAR IMMUNOLOGY
·
2021
·
IF 4.407
Yu-bo Sun,Mo Liu,Xin-sheng Fan,Li-ping Zhou,Meng-wen Li,Fang-yuan Hu,Qin-fei Yue,Yi-ming Zhang
The occurrence of asthma is closely related to environmental factors such as cigarette smoke (CS), one of the common risk factors. Environmental stimuli have the potential to activate transient receptor potential ankyrin 1 (TRPA1) and cause or aggravate asthma. The destruction of tight junctions (TJs) between airway epithelial cells by environmental stimuli in asthma has been researched. It is worth exploring whether CS can injury TJs and aggravate asthma by activating TRPA1. The objective of this study was to investigate the aggravation of CS on ovalbumin (OVA)-induced asthma related phenotypes and TJs expression in mice, and to explore the relationship between TRPA1 and the expression of TJs protein. Female wild type (WT) C57BL/6 mice, induced by OVA, CS and OVA plus CS (OVA + CS) respectively, were used to establish a 42-day asthma model, and mice with TRPA1 knockout (TRPA1 −/− ) were treated in the same way. This study detected the number of inflammatory cells in peripheral blood and bronchoalveolar lavage fluid (BALF), the levels of IL-4, IL-5, IL-13 in BALF, enhanced pause (Penh) of lung function, pathological changes and the gene and protein expressions of TRPA1 and TJs (including ZO-1, Occludin and Claudin-2) in lung tissues. Compared with normal saline (NS) group, WT mice in the OVA group and OVA + CS group were significantly higher in asthma related phenotypes. The WT-OVA + CS group also showed higher Penh value, levels of IL-5 and IL-13 in BALF and lung tissue injury scores when compared with the WT-OVA group and WT-CS group. However, WT-OVA + CS group mice had significantly larger number of neutrophils in BALF than the WT-OVA group, and had larger number of eosinophils in peripheral blood and higher levels of IL-4 in BALF than the WT-CS group. Meanwhile, compared with the WT-NS group, the expressions of TRPA1 and Claudin-2 in lung tissues increased in other three groups while their expressions of ZO-1 and Occludin decreased, among which, the WT-OVA + CS group showed more remarkable changes. Compared with the WT-OVA + CS group, mice in the TRPA1 −/− -OVA + CS showed a significant decrease in the number of inflammatory cells, levels of IL-4, IL-5 and IL-13 in BALF, Penh value and lung tissue injury score, and a downregulation of Claudin-2 expression while an upregulation of ZO-1 and Occludin expressions. In addition, the airway inflammation and injury, and the expressions of ZO-1, Occluding and Claudin-2 expressions were found with no statistic differences between TRPA1 −/− -OVA group and TRPA1 −/− -OVA + CS group. These results suggest that CS has aggravated the airway inflammation, pathological damage and destruction of TJs in airway epithelium of OVA-induced asthmatic mice, the processes of which are related to the increase of TRPA1 expression.
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Foods
·
2019
·Vol. 8
·期 8
·286
·
IF 3.011
Bin Jiang,Jiaxin Na,Lele Wang,Dongmei Li,Chunhong Liu,Zhibiao Feng
For the purpose of reducing pollution and the reutilization of salted egg whites, which are byproducts of the manufacturing process of salted egg yolks and normally treated as waste, an aqueous two-phase flotation (ATPF) composed of polyethylene glycols (PEG 1000) and (NH4)2SO4was applied to develop a simple, inexpensive and efficient process for the separation of ovalbumin (OVA) from salted egg whites. The effects of the concentration of PEG, the concentration of (NH4)2SO4, the flow rate and the flotation time on the flotation efficiency (Y) and purity (P) of OVA were investigated. A response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. An efficient separation was achieved using ATPF containing 5 mL of 80% PEG 1000 (w/w), 28 mL of 28% (NH4)2SO4(w/w), 35 mL/min of the flow rate and 30 min of the flotation time, while 2 mL of the salted egg white solution (salted eggs white (v): water (v) = 1:4) was loaded. Under the optimal conditions,YandPof OVA could reach 82.15 ± 0.24% and 92.98 ± 0.68%, respectively. The purified OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), reverse phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-nano electrospray ionisation mass spectrometry (Nano LC-ESI-MS/MS), ultraviolet spectrum (UV), fluorescence spectrum (FL) and fourier transform infrared spectroscopy (FT-IR). The results indicated that the purity of OVA obtained by ATPF was satisfactory and there was no obvious difference in the structure of the OVA separated by ATPF and the standard. The results of the functional properties revealed no significant differences between OVA obtained by ATPF and the standard in oil binding capacity, viscosity, emulsibility and foam capacity.Keywords:reutilization of food waste;salted egg white;ovalbumin;extraction;aqueous two-phase flotation
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FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Wenfei Xiong,Jing Li,Bin Li,Lifeng Wang
High intensity ultrasound (HIUS), as an efficient modification method, generally improves the interfacial properties of globular proteins. Herein, to better understand the intrinsic relationship between the foaming property and the interface behavior of HIUS-treated ovalbumin (OVA). The main purpose of this work was to investigate the correlation between the physicochemical properties of high intensity ultrasound (HIUS)-treated ovalbumin (OVA) and interfacial dilatational rheological behavior at air-water interface with different salt ionic strength. The results showed that HIUS treatment resulted in aggregation of OVA dominated by hydrophobic interaction. The size of HIUS-treated OVA gradually increased due to the charge shielding effect caused by the increase of salt ionic strength, while the surface net potential and free sulfhydryl content of the OVA particle were progressively reduced. As a result, the HIUS treated-OVA exhibited the highest diffusion rate and the amount of protein adsorbed at the air-water interface with salt ionic concentrations of 50 mM and 100 mM, respectively. On the other hand, the modified-OVA contact with the air-water interface led to an exposure of aromatic residues, the content of α–helix, β-helix, and β-turn decreased by 6.3%, increased by 10.3%, and decreased by 5.5%, respectively, and there was no salt ion strength dependence. Moreover, the conformational changes resulted in a thiol-disulfide interchange reaction between the OVA molecules at the interface, which was the main contribution to the viscoelastic properties of OVA absorbed layer. These findings provide better insights for an in-depth understanding of the foaming mechanism of ultrasound treated OVA.
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FOOD HYDROCOLLOIDS
·
2017
·
IF 4.747
Wenfei Xiong,Cong Ren,Mo Tian,Xuejun Yang,Jing Li,Bin Li
The objective of this study was to investigate the complex coacervation between ovalbumin (OVA) and carboxymethylcellulose (CMC) with different degrees of substitution (CMC 0.7 and CMC 1.2). Turbidity titration showed that complexes or coacervates could form between OVA and CMC by electrostatic interactions depending on pH changes. Specially, the critical pH values (pH c , pH φ1 ) increased with the salt ion concentration raising from 0 to 20 mM, while further increasing the ionic strength reduced the formation of complex coacervates. The ITC results also showed that when C NaCl = 20 mM, the complex coacervation between OVA and CMC exhibited the highest stoichiometric ratio and binding constant. When C NaCl ≥200 mM, the complex coacervation changed from spontaneous exothermic (ΔG<0, ΔH<0) to endothermic (ΔG<0, ΔH>0) due to the shielding effect by high concentration of salt ions. In addition, dynamic rheological properties showed that the OVA/CMC coacervates displayed the strongest elastic modulus at C NaCl = 20 mM, and the elastic modulus of all samples were greater than the viscous modulus. These findings all suggested a salt-enhanced effect at lower salt concentrations or a salt-reduced effect at higher salt concentrations. On the other hand, the stoichiometric and viscoelastic properties of OVA/CMC 1.2 coacervates were greater than OVA/CMC 0.7 coacervates, indicating that CMC 1.2 had stronger protein binding capacity owing to higher charge density.
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