Journal of Pharmaceutical Analysis
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2021
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IF 4.769
Guangjun Feng,Yang Yang,Jiantao Zeng,Jun Zhu,Jingjian Liu,Lun Wu,Zhiming Yang,Guanyi Yang,Quanxi Mei,Qinhua Chen,Fengying Ran
Rutin, a flavonoid found in fruits and vegetables, is a potential anticancer compound with strong anticancer activity. Therefore, electrochemical sensor was developed for the detection of rutin. In this study, CoWO 4 nanosheets were synthesized via a hydrothermal method, and porous carbon (PC) was prepared via high-temperature pyrolysis. Successful preparation of the materials was confirmed, and characterization was performed by transmission electron microscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. A mixture of PC and CoWO 4 nanosheets was used as an electrode modifier to fabricate the electrochemical sensor for the electrochemical determination of rutin. The 3D CoWO 4 nanosheets exhibited high electrocatalytic activity and good stability. PC has a high surface-to-volume ratio and superior conductivity. Moreover, the hydrophobicity of PC allows large amounts of rutin to be adsorbed, thereby increasing the concentration of rutin at the electrode surface. Owing to the synergistic effect of the 3D CoWO 4 nanosheets and PC, the developed electrochemical sensor was employed to quantitively determine rutin with high stability and sensitivity. The sensor showed a good linear range (5–5000 ng/mL) with a detection limit of 0.45 ng/mL. The developed sensor was successfully applied to the determination of rutin in crushed tablets and human serum samples.
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FOOD RESEARCH INTERNATIONAL
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2019
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IF 3.579
Wei Xu,Nan Zhang,Zhentao Zhang,Pu Jing
Anthocyanins exist likely as complexes with flavones/metal ions in some plants. However, few studies indicate the complexation effects on bioactivities, i.e. antioxidant activities. The aim of this study was to evaluate effects of complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G) with rutin/Mg(II) on intrinsic antioxidant enzymes and mRNA expressions. All complexes exhibited cellular antioxidant activity in RAW264.7 cells, whereas half maximal effective concentration (EC 50 ) values of CY3D5G complexes were significantly lower than its alone. Combination indexes of CY3D5G-rutin, CY3D5G-Mg(II), and CY3D5G-rutin-Mg(II) complexes were < 1 ( p < .05), indicating of synergistic effects. Additionally, CY3D5G-Mg(II) rather than others significantly enhanced total antioxidant capacity, superoxide dismutase activity, and glutathione peroxidase activity while reducing malonic dialdehyde contents in H 2 O 2 -induced RAW264.7 cell model ( p < 0.05), which were further confirmed with the up-regulated mRNA expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), and the down-regulated p21 ( p < 0.05). Our results demonstrated that CY3D5G and its complexes might enhance cellular antioxidant activities via the Nrf2/HO-1 signaling pathway. Additionally, a synergistic antioxidant effect of CY3D5G in the present of rutin/ Mg(II) as regular food components was evident, suggesting anthocyanin might interact with dietary components such as Mg(II) or rutin in food matrix or biological systems and exhibit a stronger antioxidant activity than it alone. The Mg (II) that exhibited no antioxidant activities alone shows potential as a co-adjuvant for the anthocyanin antioxidant activity.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2020
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IF 1.415
Li Jun Li,Xiao Qing Liu,Xi Ping Du,Ling Wu,Ze Dong Jiang,Hui Ni,Qing Biao Li,Feng Chen
Isoquercitrin is a flavonoid with important applications in the pharmaceutical and nutraceutical industries. However, a low yield and high production cost hinders the industrial preparation of isoquercitrin. In the present study, isoquercitrin was prepared by biotransformation of rutin using α-L-rhamnosidase from Aspergillus niger JMU-TS528 combined with high-speed countercurrent chromatography (HSCCC) purification. As a result, the optimum transformation pH, temperature, and time were pH 4.0, 60 °C, and 60 min, respectively. The Km and Vmax were 0.36 mM and 0.460 mmol/min, respectively. For isoquercitrin production, the optimal rutin concentration and transformation time were approximately 1000 mg/L and 60 min. The rutin transformation rate reached 96.44%. The isoquercitrin was purified to a purity of 97.83% using one-step purification with HSCCC. The isoquercitrin was identified using UPLC-Q-TOF-MS. The comprehensive results indicated that the biotransformation procedure using the α-L-rhamnosidase from A. niger JMU-TS528 combined with HSCCC was a simple and effective process to prepare isoquercitrin, which might facilitate the production of isoquercitrin for industrial use.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-12
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IF 5.3
Li Lin,Liu Lanlan,Zhou Jianmei,Gu Chen,Wu Xiongzhi,Lei Chenghong,Yan Liqiang
A simple and rapid electrochemical method based on a composite of cadmium telluride quantum dots (CdTe QDs) and MXene is developed for the simultaneous determination of rutin and quercetin in food samples. The CdTe QD-MXene composite is synthesized via the in situ growth of CdTe QDs on MXene, which serves as a carrier and enhances electrical conductivity. Incorporating CdTe QDs into MXene interlayers effectively prevents agglomeration in MXene and provides more active sites for electrochemical determination. The developed electrochemical method can successfully determine rutin and quercetin, both individually and simultaneously, in aqueous solutions while achieving high stability and selectivity. Notably, the prepared sensor exhibits limits of detection of 3.300 × 10 −8 and 3.268 × 10 −7 M for the simultaneous determination of rutin and quercetin, respectively. Moreover, the sensing platform is used for the determination of rutin in buckwheat, locust rice, and apples, with results well comparable to those obtained using ultraviolet spectroscopy. Finally, the proposed sensor is employed to monitor the hydrolysis of rutin into quercetin in buckwheat using an electrochemical method for the first time. This study provides new ideas for the application of electrochemical sensors in food and drug science. Graphical
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ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Jing Wang,Nuli Xie,Shan Lu,Bo Xiao,Yangqin Ou,Qingnian Li,Dexian Liu,Wenhu Zhou
Intracerebral hemorrhage (ICH) presents a formidable challenge due to its high mortality and disability rates, primarily attributed to cerebral hematoma formation and ensuing neuroinflammation. Swift hematoma removal is paramount for prognosis, yet existing interventions carry risks and limitations. Notably, elevated CD47 expression on hematoma-associated RBC triggers a “don't eat me” signal, impeding hematoma clearance, while microglial/macrophage erythrophagocytosis exacerbates oxidative stress and the RBC lysate evokes neuroinflammation. To address this conundrum, a multifunctional nanomedicine (TD-CFR), employing DNA tetrahedra (TD) as a carrier for ICH treatment is introduced. The investigations reveal that CpG enhances the phagocytosis of CD47-expressing RBC by microglia/macrophages via lipid metabolism modulation. Integration of CpG into TD preserves its pro-phagocytic efficacy, while TD's double-stranded region enables efficient encapsulation of Rutin, a potent anti-inflammatory and antioxidant flavonoid. Capitalizing on disrupted blood-brain barrier integrity at the hemorrhage site, TD-CFR achieves robust enrichment within cerebral hematoma post-intravenous administration, augmented by folate receptor-mediated targeting of microglia/macrophages. Efficacy assessments in mouse and rabbit ICH models confirm TD-CFR's therapeutic benefits, including hematoma clearance, neuroinflammation suppression, and brain function restoration. Leveraging TD's high biosafety profile and dual active ingredient loading capacity, the study unveils a promising drug treatment paradigm for ICH.
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JOURNAL OF THERMAL BIOLOGY
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2025
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IF 2.9
Longfei Ma,Huijuan Liu,Zhongyu Ge,Bing Bai,Jianle Zhao,Shun Chen,Binbin Zhou,Jiaqi Zhang,Tian Wang,Chao Wang
Heat stress (HS) leads to abnormal lipid metabolism in broilers, while rutin as a natural flavonoid with multiple biological functions shows potential to regulate antioxidant capacity and lipid metabolism. Therefore, this study aimed to investigate the effects of rutin supplementation on antioxidant and lipid metabolism functions in heat-stress broilers and to further explore its potential mechanisms. A total of 192 male broilers were randomly divided into three groups and treated with normal control (CON), heat stress (34 °C, HS), and HS with 500 mg kg -1 rutin supplementation (HS + Rutin), respectively. The results showed that dietary supplementation of 500 mg kg -1 rutin could significantly reverse the HS-induced liver damage. Rutin also mitigated HS-induced increases in liver triglyceride content and decreases in high-density lipoprotein and fatty acid binding protein contents. This might be achieved by increasing the expression of PPARα and decreasing the expression of SREBP1, which regulated the expression of downstream factors (such as CPT1, ACOX1, and ACC). Compared with the HS group, rutin increased mitochondrial DNA copy number and maintained mitochondrial homeostasis by regulating the expression of related genes (such as AMPK, PGC1-α, and SIRT3). In addition, rutin increased the activity of antioxidant enzymes, decreased the content of malondialdehyde, and activated the nuclear respiratory factor 2 (Nrf2) signaling pathway in the liver, which alleviated HS-induced oxidative stress. The effect of rutin in alleviating abnormal lipid deposition in HS broiler livers were correlated with indicators that reflected the antioxidant capacity and mitochondrial homeostasis. In conclusion, dietary 500 mg kg -1 rutin alleviates heat stress-induced hepatic abnormal lipid metabolism of broilers via improving antioxidant capacity to maintain mitochondrial homeostasis.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
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IF 4.7
Jing Tian,Feng Xiao,Yezhu Xu,Yuen Chen,Chenli He,Su Jiang,Yunping Tang
α-Monoglucosyl rutin (α-GR) is a structurally optimized rutin derivative, and its higher water solubility and bioavailability may help it exert better pharmacological effects in vivo . The present study examined the protective effects and potential mechanisms of α-GR on cyclophosphamide (CTX)-induced colonic injury in mice. The findings showed that α-GR significantly reduced serum levels of lipopolysaccharide (LPS) and diamine oxidase (DAO), effectively alleviating the histopathological damage in colon tissues caused by CTX exposure. Additionally, α-GR maintained the integrity of intestinal epithelial cells by promoting the secretion of secretory immunoglobulin A (sIgA) and upregulating the expression of tight junction proteins (Claudin-1 and ZO-1), while also reducing the levels of colonic pro-inflammatory factors and oxidative stress. In terms of gut microbiota, 16S rRNA gene sequencing and metabolomic analyses revealed that α-GR could effectively reverse the gut metabolic disorders and microbiota structural changes induced by CTX, promoted the growth of beneficial bacteria like norank_f_Muribaculaceae , Bacteroides, and Alloprevotella , and reduced the relative abundance of pathogenic bacteria ( Desulfovibrio ), while also revealing the complex correlation between them. In terms of mechanism research, α-GR further attenuated intestinal damage by regulating the TLR4/NF-κB and Nrf2/HO-1 pathways. This provides new ideas for the development of functional dietary supplements to treat CTX-associated intestinal toxicity.
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RESEARCH IN VETERINARY SCIENCE
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2025
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IF 1.8
Mona H. Hafez,Neveen R. Ashoura,Shymaa A. Khatab,Tasnim S. Waheeb,Hebatallah M. Saad,Kariman A. Esmail
Our research aimed to explore the performance, immunity, antioxidants, gene expression, and histopathology of broilers grown at standard temperatures vs those subjected to heat stress (HS) with rutin supplementation. Two hundred forty-one-day-old chicks were randomly allocated into 4 groups. Control group; Rutin group was administered rutin (500 mg/kg food) at ambient temperature; HS group, birds exposed to HS (35 ± 2 °C) for 14 consecutive days; HS + Rutin group was administered the same dosage of rutin, and the birds were then exposed to HS. Molecular docking revealed high binding affinity of rutin toward the growth factors myostatin, insulin-like growth factor receptor-1 (IGFR-1), growth hormone receptor (GHR), heat Shock protein 90 (HSP90), heat Shock factor-1(HSF-1) and HSP70. Rutin not only improved the body weight and feed conversion ratio, but it also considerably reduced the negative effects of HS on growth performance. It also led to decrease in malondialdehyde and pro-inflammatory cytokine levels of interleukin 2 (IL-2), tumor necrosis factor (TNF-α), and IL-6, together with an increase in the antioxidant enzymes CAT, GPX, and SOD activity in addition to a considerable improvement in immunoglobulin (IgM, IgG, and IgA) secretion during HS. Interestingly on molecular level, rutin caused an upregulation in expression of HSP90, HSF-1, HSP70, growth related genes (myostatin, IGFR-1, GHR), and downregulation of immune-related genes chemokines [Lipopolysaccharide-induced TNF-α factor (LITAF), IL-1β, IL-6 gene expression. These changes are further confirmed by histopathological investigation that revealed improvement in hepatic and breast muscle histoarchitecture, increased glycogen and decreased IL-1β immunoexpression after rutin supplementation in HS. In conclusion, rutin is an efficient feed additive that improves antioxidant status, growth, and immunological function in HS.
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BIOCHEMICAL ENGINEERING JOURNAL
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2023
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IF 3.9
Jinyue Chai,Xue Chen,Chengcheng Jin,Fang Chai,Miaomiao Tian
In this study, AgCl was used as the carrier matrix, TiO 2 was introduced on the substrate surface, carboxyphenylboronic acid (CPBA) was used as the boronate affinity functional monomer , and Rutin (RT) as template to prepare molecular imprinted adsorbent AgCl@TiO 2 @B-MIPs (B-MIPs). The effects of synthesis conditions, solid-phase extraction (SPE) conditions, selectivity, competitiveness, reproducibility, and reusability were evaluated in detail. Under the optimum adsorption conditions, the maximum adsorption capacity is 31.70 mg g −1 . The utility of B-MIPs as a SPE adsorbent for RT was supported by an abundance of pore structure, as well as boric acid affinity sites that facilitate rapid binding of adsorbents to templates. The linear range is 0.05–50 mg L −1 , and the limit of detection (LOD) is 0.023 mg L −1 . The method was used for the determination of RT in sunscreen and isolation cream by high-performance liquid chromatography (HPLC). The target analytic recoveries are in the range of 84.7–98.0% (relative standard deviation is less than 2.4%, n = 3). Furthermore, this work provides a potential possibility to detect other cis -diol containing analytes in the complex matrix.
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Shao-Bin He,Hui-Jing Yang,Liu Yang,Hamada A. A. Noreldeen,Hua-Ping Peng,Hao-Hua Deng,Wei Chen,Guo-Lin Hong
Coenzyme can work with a natural enzyme to achieve the enzymatic catalytic process. Inspired by nature, herein rutin was found to regulate the peroxidase-like activity of Fe-doped silicon nanodots (Fe-OSiNDs) acting as a coenzyme. To be specific, the peroxidase-like activity of Fe-OSiNDs can be activated by rutin at a low concentration (<200 µM), which accelerated the oxidation process between 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and H 2 O 2 . Possibly as a reason, rutin at a low concentration could be easily attracted and oxidized by Fe 3+ in Fe-OSiNDs and then the produced Fe 2+ reacted with H 2 O 2 to form the hydroxyl radicals around the surface of Fe-OSiNDs thereby contributing to an enhancement. More interestingly, the addition of β-glucuronidase can hydrolysis rutin into quercetin and suppress the peroxidase-like activity of rutin-treated Fe-OSiNDs because quercetin is a sugar-free flavonoid with higher anti-oxidant activity than rutin. Thus, a colorimetric sensor for β-glucuronidase could be established. This sensor realizes a linear ranging from 2 to 40 nM (0.58 to 11.6 μg/mL) towards β-glucuronidase with a detection limit of 0.6 nM (0.174 μg/mL). The feasibility of this sensor for β-GUS detection in Escherichia coli has been realized. This work not only provides an interesting concept for coenzyme-dependent nanozyme and expands the promising use of silicon nanomaterials.
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Journal of Environmental Sciences
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2022
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IF 6.796
Yunhao Zhang,Yi Qian,Ming Zhang,Weichuan Qiao
The wastewater discharge from the process of chrome plating, which contains 6:2 chlorinated polyfluorinated ether sulfonate (F-53B) and chromium (Cr), may be toxic to biofilm. In this study we found that the biofilm formed by Pseudomonas aeruginosa PAO1 was inhibited by exposure to a combination of F-53B and Cr(VI). The combined pollution damaged the cell membranes and the structure of the biofilm, and inhibited the production of the Pseudomonas quinolone-based signal, which affected biofilm formation. Moreover, the secretion of extracellular polymeric substances decreased as a result of this combined exposure. Exposure to F-53B and Cr(VI) individually or in combination could induce the excessive accumulation of intracellular reactive oxygen species (ROS), and the ROS positive rate of the bacteria increased under the treatment with 0.2 mmol/L of Cr(VI) and 250 nmol/L of F-53B, respectively. In addition, the activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced for scavenging ROS in the bacteria that were exposed to Cr(VI) and F-53B. As an antioxidant, rutin was used to repair the toxicity of Cr(VI) and F-53B towards the biofilm formed by the bacteria. When rutin was added to the bacteria medium, with either Cr(VI) or F-53B as pollutant, or with the combined pollutants, the extracellular protein content of the bacteria recovered to 0.84, 0.94, and 0.85 times that of the control, respectively. Meanwhile, the accumulation of ROS and the activities of SOD and CAT decreased, which indicated that the addition of rutin can alleviate the oxidative stress and promote the antioxidant stress system.
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Frontiers in Bioengineering and Biotechnology
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2021
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IF 5.89
Yang Shilong,Sun Lu,Song Zhiwen,Xu Li
Rutin (R), a representative flavonoid found in various biomasses, can be used to prepare different fluorescent sensors for environmental, biological and medical fields. In this work, the natural R in Sophora japonica was extracted and purified to prepare fluorescent-responding sensor systems intended to recognize copper ions with both strong selectivity as well as appropriate sensitivity. Results showed that neat R had no obvious fluorescent emission peak in PBS buffer solution. However, when R and (2-hydroxypropyl)-β-cyclodextrin (CD) were introduced within buffer solution, fluorescent emission intensity was significantly increased due to the resultant R-CD inclusion complex. In addition, the formed R-CD inclusion complex was shown to behave as the aforementioned fluorescent sensor for copper ions through a mechanism of quenched fluorescent emission intensity when R-CD became bound with copper ions. The binding constant value for R-CD with copper ions was 1.33 × 106, allowing for quantification of copper ions between the concentration range of 1.0 × 10–7–4.2 × 10–6mol⋅L–1. Furthermore, the minimum detection limit was found to be 3.5 × 10–8mol⋅L–1. This work showed the prepared R-CD inclusion complex was both highly selective and strongly sensitive toward copper ions, indicating that this system could be applied into various fields where copper ions are of concern.
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