查找使用阿拉丁产品的科研文献

Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole

Ding Chao-Yang,Ma Yu-Meng,Li Bin,Wang Yun,Zhao Le,Peng Jiang-Nan,Li Mao-Ye,Liu Su,Li Shi-Guang

Plant essential oils, with high bioactivity and biodegradability, provide promising alternatives to synthetic pesticides for pest control. Trans-anethole is the major component of essential oil from star anise, Illicium verum Hook. The compound has a strong contact toxicity against the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), which is a major insect pest of many vegetables and crops. However, little information is known about how M. persicae responds to trans-anethole at the molecular level. We conducted a comparative transcriptome analysis of M. persicae in response to a LD50 dose of trans-anethole. A total of 559 differentially expressed genes were detected in the treated individuals, with 318 genes up-regulated, and 241 genes down-regulated. Gene ontology (GO) analysis revealed that these genes were classified into different biological processes and pathways. We also found that genes encoding ATP-binding cassette (ABC) transporters, DnaJ, and cuticle proteins were dramatically up-regulated in response to trans-anethole. To study the function of these genes, we performed RNA interference (RNAi) analysis. Knockdown of an ABC transporter gene (ABCG4) and a DnaJ gene (DnaJC1) resulted in a significantly increased mortality rate in M. persicae following trans-anethole exposure, indicating the involvement of these two genes in the toxicity response to trans-anethole. The findings provide new insights into the mechanisms of M. persicae in coping with plant essential oils.

Identification and characterization of both cis- and trans-regulators mediating fenvalerate-induced expression of CYP6B7 in Helicoverpa armigera

Yun Huang,Li Xu,Junyue Zheng,Peizhuo Wu,Yu Zhang,Lihong Qiu

As we known, inducibility is an important feature of P450 genes. Previous studies indicated that CYP6B7 could be induced and involved in fenvalerate detoxification in Helicoverpa armigera . However, the regulatory mechanism of CYP6B7 induced by fenvalerate is still unclear. In this study, CYP6B7 promoter of H. armigera was cloned and the cis -acting element of fenvalerate was identified to be located between −84 and − 55 bp of CYP6B7 promoter. Subsequently, 33 candidate transcription factors ( CYP6B7 -fenvalerate association proteins, CAPs) that may bind to the cis -acting element were screened and verified by yeast one-hybrid. Among them, the expression levels of several CAPs were significantly induced by fenvalerate. Knockdown of juvenile hormone-binding protein-like (JHBP), RNA polymerase II-associated protein 3 (RPAP3), fatty acid synthase-like (FAS) and peptidoglycan recognition protein LB-like (PGRP) resulted in significant expression inhibition of CYP6B7 , and increased sensitivity of H. armigera to fenvalerate. Co-transfection of reporter gene p (−84/−55) with pFast-CAP showed that JHBP, RPAP3 and PGRP could significantly increase the activity of CYP6B7 promoter. These results suggested that trans -acting factors JHBP, RPAP3 and PGRP may bind with cis -acting elements to regulate the expression of CYP6B7 induced by fenvalerate, and play an important role in the detoxification of H. armigera to fenvalerate.

Sensitive Voltammetric Sensor for Evaluation of trans-resveratrol Levels in Wines based on Poly(L-lysine) Modified Electrode

Congming Li,Zhou Yanli,Ye Baoxian,Xu Maotian

Abstract A novel, economic, and highly sensitive electrochemical sensor for the determination of trans -resveratrol ( RES ) was fabricated by electropolymerization of poly(L-lysine) films onto glassy carbon electrode ( GCE ) surface. The electrochemical behavior of RES in Britton–Robinson buffer solution (pH 2.0) on the poly(L-lysine)/GCE was investigated by cyclic voltammetry and linear sweep voltammetry for the first time. Results showed that the detection range of the proposed sensor to RES was from 0.20 to 12.0 μM with the minimum detectable concentration of 0.06 μM. The reported electrochemical sensor also exhibited high selectivity, good reproducibility, and long-term stability for RES detection. In addition, the proposed method can be employed for the determination of RES in red wines with satisfactory results. The proposed electrochemical strategy presents valuable information related to evaluation of RES levels in real samples.

Polymeric nanoparticles based on chitooligosaccharide as drug carriers for co-delivery of all-trans-retinoic acid and paclitaxel

Jing Zhang,Jian Han,Xiuli Zhang,Jing Jiang,Maolei Xu,Daolai Zhang,Jingtian Han

An amphiphilic all- trans -retinoic acid (ATRA)–chitooligosaccharide (RCOS) conjugate was synthesized to form self-assembled polymeric nanoparticles to facilitate the co-delivery of ATRA and paclitaxel (PTX). The blank RCOS nanoparticles possessed low hemolytic activity and cytotoxicity, and could efficiently load PTX with a drug loading of 22.2% and a high encapsulation efficiency of 71.3%. PTX-loaded RCOS nanoparticles displayed a higher cytotoxicity to HepG2 cells compared to PTX plus ATRA solution when corrected by the accumulated drug release. Cellular uptake profiles of RCOS nanoparticles were evaluated via confocal laser scanning microscope and flow cytometry with FITC as a fluorescent mark. The RCOS nanoparticles could be rapidly and continuously taken up by HepG2 cells via endocytosis and transported into the nucleus, and the uptake rates increased with particle concentration. These results revealed the promising potential of RCOS nanoparticles as drug carriers for co-delivery of ATRA and PTX or other hydrophobic therapeutic agents.

Spectroscopic evidences of toxic trans-crotonaldehyde trapped and transformed by resveratrol to prevent the damage of mitochondrial DNA

Yanbin Su,Lei Chen,Yanwen Su,Zhengqiang Li,Chuang Zhang,Tongxing Mu

The purpose of this study is to probe the spectroscopic evidences of toxic trans-crotonaldehyde (TCA) trapped and transformed by resveratrol (Res) to prevent the damage of mitochondrial DNA. In aldehyde dehydrogenase (ALDH) at the different pH or mitochondria, the spectroscopic characteristics of TCA trapped and transformed by Res were observed by means of both UV-vis and Raman spectra. When Res interacted with TCA, TCA peak at 316 nm immediately disappeared while Res main peak at 305 nm and shoulder peak at 320 nm were dramatically changed, Raman peaks of TCA at 1,688 cm −1 assigned to CHO and 1,641 cm −1 affiliated to CC were strikingly shifted, Raman peaks of Res itself were significantly displaced or disappeared, especially in mitochondria and ALDH at different pH. The active groups of Res were the  OH at C 5 and C 10 . The results of theoretical calculations are in agreement on the whole with the experimental data. The Res plays undoubtedly an important role via the structural change in TCA. The toxic CHO of TCA was effectively trapped and transformed by Res by means of itself  OH at C 5 and C 10 . The mitochondrial alkaline microenvironment and ALDH promoted the elimination of toxic TCA. © 2017 IUBMB Life, 69(7):500–509, 2017

Transparent and organosoluble cardo polyimides with different trans/cis ratios of 1,4-diaminocyclohexane via aromatic nucleophilic substitution polymerization

Yongmei Tang,Lan Li,Kai Ma,Guofei Chen,Wei Wang,Xingzhong Fang

Two series of cardo polyimides were prepared from 1,4-bis(4-fluorophthalimide)cyclohexane with different trans / cis ratios and phenolphthalein/ o -cresolphthalein via aromatic nucleophilic substitution reaction. The inherent viscosities of the synthesized polymers were found to be 0.55–0.66 dL g −1 in N , N ′-dimethylacetamide. The cardo polyimides showed excellent solubility in organic solvents, high glass transition temperatures ( T g ) of 275–312 °C and moderate thermal stability with 5% weight loss temperatures ( T d5% ) of 415–441 °C in nitrogen and 370–436 °C in air. The polyimide films exhibited high optical transparency with cut-off wavelengths of 350–355 nm and moderate mechanical properties. The different properties of the polymers caused by trans and cis configurations of 1,4-diaminocyclohexane were also investigated. It was found that with an increasing content of trans configuration of 1,4-diaminocyclohexane in the polyimide backbone, T g of the polyimides increased as well as T d5% , while the solubility gradually decreased. The polyimide films had good optical transparency regardless of trans / cis configuration. © 2018 Society of Chemical Industry

A combination of cuminaldehyde with citral- / trans-2-hexen-1-al alleviates green mold disease in citrus fruit

Reymick Okwong Oketch,Duan Bin,Feng Zhao,Zhang Yong Hua,Ouyang Qiuli,Kaaya Archileo N.,Tao Nengguo

Management of Penicillium digitatum requires use of highly effective fungicides that are not prone to drug resistance and are safe to humans and the environment. This study examined the fungicidal potential of combinations of cuminaldehyde (CA) + trans-2-hexen-1-al (TH) and CA + Citral. Both CA + TH and CA + Citral exhibited enhanced fungitoxicity against P. digitatum than CA, citral or TH alone. The minimum inhibitory concentration (MIC) of CA + Citral consisted of 0.13 ml/L CA and 0.3 ml/L citral, representing a synergistic interaction. MIC of CA + TH consisted of 0.12 ml/L CA and 0.15 ml/L TH, representing an additive interaction. The minimum fungicidal concentration (MFC) of CA + Citral and of CA + TH consisted of 0.26 ml/L CA and 0.6 ml/L citral, and 0.18 ml/L CA and 0.24 ml/L TH, respectively. 10×MFC CA + Citral in Tween-80 (WCI) delayed onset of hyphal growth in fruits inoculated with P. digitatum to day 5 compared to day 4 in the 10×MFC CA + TH in Tween-80 (WCT) treatment, and day 3 for the control samples. Daily green mold disease incidence and disease severity were much lower in WCI and WCT treatments than in the 10×MFC CA in Tween-80 (WCA) treatment and in control samples. Our results show that WCI was much fungitoxic against P. digitatum than WCT and WCA, its effect being comparable to that of imazalil. Additionally, at 25 ± 2 °C and 85–90% relative humidity, both WCI and WCT markedly preserved fruit quality by day 6 of treatment. WCI and WCT are thus, safe, cheap and highly effective alternatives to chemical fungicides being used to control postharvest green mold in citrus fruits.

Inhibitory effects of trans-cinnamaldehyde on Harman and Norharman formation in spiced pork shoulders: Mechanisms and chemical models

Yuxia Zhang,Guangming Yao,Yongliang Ma,Yajun Zhou

This study investigates the inhibitory effects of trans -cinnamaldehyde (TCA) on Harman and Norharman formation in spiced pork shoulders and chemical models, elucidating the underlying mechanisms. Our results demonstrate that TCA significantly reduces the levels of these compounds, with optimal inhibition observed at 0.1 mg/mL TCA in spiced pork shoulders (80.2 % reduction in Harman, 94.7 % reduction in Norharman) and 0.1 mmol TCA in chemical models (89.1–90.2 % reduction in Harman, 88.7–99.2 % reduction in Norharman). For the first time, two potential reaction mechanisms by which TCA inhibits the formation of HAAs are proposed: TCA can react with tryptophan to form adducts or directly scavenge HAAs. Notably, quantum chemical theoretical calculations indicate that the reaction energy barriers (108.3 kJ/mol and 156.0 kJ/mol) for the TCA-tryptophan reaction to form an aldimine were significantly lower than those (123.2 kJ/mol and 187.2 kJ/mol) for the glucose-tryptophan reaction to form a Schiff bases. Therefore, Compared to the glucose-tryptophan reaction, the TCA-tryptophan reaction is more likely to occur. Mass spectrometry analysis further confirms the presence of TCA-tryptophan adduct compounds, supporting the proposed mechanisms. These findings may provide more theoretical foundation researches for the development and research of HAAs inhibitors in the future.

Insecticidal activity of essential oil of Cinnamomum cassia and its main constituent, trans-Cinnamaldehyde, against the booklice, Liposcelis bostrychophila

XC Liu,J Cheng,NN Zhao,ZL Liu

Trans-Anethole Alleviates DSS-Induced Ulcerative Colitis by Remodeling the Intestinal Flora to Regulate Immunity and Bile Acid Metabolism

Xu-Hui Li,Li Liu,Wen-Zhong Wu

Ulcerative colitis (UC) is the most common inflammatory bowel disease (IBD); it is incurable, and the treatment is expensive. Trans-anethole (TA), the main component of fennel, exhibits various biological activities. An increasing number of studies have demonstrated the efficacy of herbal active ingredients in the treatment of UC. This study aimed to investigate the effect and mechanism of TA in UC. In this study, we have experimented on mice with dextran sulfate sodium salt (DSS)-induced UC. The TA group was gavaged with 62.5 mg/kg TA by gavage once daily on days 8–14. To observe the effect of TA on the colon tissue, various investigations were performed, including western blot and immunohistochemistry for intestinal barrier protein expression, TUNEL staining for apoptosis, western blot, and ELISA for inflammation level, flow cytometry for Th17/Treg, LC–MS for blood bile acid content, GC–MS for blood fatty acid content, and 16s RNA for intestinal contents. TA alleviated weight loss in mice with UC; increased colon length; alleviated intestinal mucosal damage; upregulated claudin-1, occludin, and ZO-1 protein expression levels; reduced inflammatory factors in the colon and serum; and alleviated apoptosis. TA reduced fatty acid and bile acid levels by inhibiting colony abundance and reducing Th17/Treg cell differentiation in the colon. We found that TA alleviates DSS-induced UC by remodeling the intestinal flora to regulate immunity and bile acid metabolism.

Coherent experimental and simulation approach to explore the cis/trans isomeric effects of cobalt thioporphyrazines on their photocatalytic performance

Changjun Yang,Shuai Xu,Fanchang Peng,Wenyao Zhou,Quan Zhou,Xi Chen,Bingguang Zhang,Kejian Deng

The cis / trans isomeric effects of cobalt thioporphyrazine (CoPz) bearing 1,4-dithiin (A) and bis(butylthio) (B) groups on their photocatalytic performance were investigated by photocatalytic selective aerobic oxidation of 5-hydroxymethylfurfural (HMF) in aqueous solution under simulated sunlight. The results demonstrated that the cis -CoA 2 B 2 consistently showed higher photocatalytic activity than the trans -CoA 2 B 2 , especially in alkaline solution. Meanwhile, the cis -CoA 2 B 2 favored oxidizing HMF to 2,5-furandicarboxylic acid (FDCA), while the trans -CoA 2 B 2 preferred yielding 2,5-diformylfuran (DFF). Under alkaline conditions, the yield of FDCA can reach 73% with a selectivity of 95% in the presence of cis -CoA 2 B 2 after reacting 14 h, while 37% of DFF yield with a selectivity of 81% can be achieved in the presence of trans -CoA 2 B 2 after reacting 12 h. The relationship between the structure of cis / tr ans isomers and their photocatalytic performance was further explored by density functional theory (DFT) calculations. The active species originated from cis -CoA 2 B 2 and trans -CoA 2 B 2 were also examined by trapping experiments.

A ratiometric and trans-cleavage-triggered feedback CRISPR/Cas12a cascade for amplification-free MicroRNA detection using a multifunctional switch

Yaru Zhang,Yongchang Sun,Jing Yuan,Lihua Ding,Leiliang He,Yongjun Wu,Songcheng Yu

MicroRNAs (miRNAs), as crucial regulators of gene expression, play significant roles in cancer development. Among them, miRNA-21 has been recognized as a promising biomarker for the diagnosis and prognosis evaluation of multiple cancers. However, existing CRISPR/Cas12a-based miRNA detection methods generally require reverse transcription and pre-amplification steps, which complicate the operational workflow and are prone to introducing cross contamination. Furthermore, the dependence on single-fluorescence signal detection makes them vulnerable to environmental and instrumental fluctuations, which results in risk of false-positive outcomes. Herein, we developed a ratiometric and sensitive CRISPR/Cas12a system with trans-cleavage-triggered feedback signal amplification for amplification-free detection of miRNA-21. The strategy employs a novel multifunctional switch probe that integrates target recognition, signal transduction, and cascade amplification within a unified system. A trans-cleavage-triggered feedback signal amplification circuit was constructed to enhance detection sensitivity without the need for additional enzyme systems. Moreover, a ratiometric fluorescence signal was implemented by calculating the fluorescence intensity ratio between CY5 and Auramine O, which effectively minimizes interference from environmental and instrumental variations and substantially improves detection accuracy and reliability. Experimental results demonstrate that the method achieves a detection limit of 1.28 nM, along with excellent specificity, accuracy, and reproducibility. In real sample analysis, differential expression levels of miRNA-21 in lung adenocarcinoma cells (A549) and non-cancerous adult bronchial epithelial cells (BEAS-2B) was observed, which highlights its potential value for clinical diagnostics.