ACS Applied Nano Materials
·
2025
·
IF 5.5
Hang Su,Shuo Huang,Dewen Su,Carlos Garcia Nuñez,Hadi Heidari,Haopeng Zhang,Jingming Lan,Xin Gao,Hongyan Yue
It is highly important to efficiently recognize and detect chiral molecules in drug development and life sciences. In this paper, an electrochemical chiral sensor for the detection of L-tryptophan (l-Trp), based on l-cysteine (l-Cys)@island-like Co3S4 on Co3O4 nanosheet arrays heterostructure/carbon paper (l-Cys@Co3S4–Co3O4/CP), is presented. Co-MOF triangular nanosheets with a thickness of ∼200 nm are first grown on the CP. After calcination and partial sulfidation, the Co3S4–Co3O4 heterostructure forms. Finally, l-Cys, as a chiral selector, is uniformly decorated on the heterostructure. The island-like heterostructure exhibits superior electrical conductivity and could potentially provide active sites for the absorption of l-Cys. The l-Cys@Co3S4–Co3O4/CP electrode is utilized for the electrochemical recognition and detection of L-tryptophan (l-Trp) via a differential pulse voltammetry (DPV) method. It demonstrates a recognition efficiency of 1.50 and shows high sensitivity (0.33 μA·μM–1) and a low detection limit (0.021 μM) for l-Trp within the concentration range of 0.06–2 mM. The electrode has good anti-interference ability, reproducibility, and repeatability. Moreover, it exhibits good detection performance of l-Trp in biological samples, with recovery ranging from 96% to 105%, demonstrating excellent potential in clinical applications.
研究领域:
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Mingwei Peng,Jicheng Fu,Yingjie Song,Meiqi Lu,Baoshun Zhang
Cerebral ischemia-reperfusion (I/R) injury involves brain tissue damage caused by a temporary interruption of blood flow followed by reperfusion, leading to inflammation, oxidative stress, and neuronal death. Diosmetin, a flavonoid with known anti-inflammatory and antioxidant properties, has been shown to mitigate I/R injury, but its impact on systemic inflammation is not fully understood. In this study, we investigated its protective effects on I/R injury and the mechanisms mediated through the microbiota-gut-brain axis. Using MCAO-induced rats and OGD/R-induced PC12 cells, we employed multiomics sequencing and molecular biology techniques. The results showed that the IL-6/pJAK1/pSTAT3 signaling pathway was the key mechanism influenced by tryptophan metabolites. Diosmetin alleviated intestinal and systemic inflammation by modulating tryptophan metabolism and gut microbiota dysbiosis. Microbiota transplantation from diosmetin-treated rats produced similar protective effects. In conclusion, diosmetin mitigated I/R injury by influencing tryptophan metabolism through the microbiota-gut-brain axis, offering potential therapeutic strategies for I/R injury.
研究领域:
神经科学
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药理学
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微生物学
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代谢组学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2025
·
IF 2.6
Fujian Li,Min Li,Zhibo Zhu,Yuan Liu,Dandan Han
L-tryptophan, an essential amino acid for protein synthesis and neurotransmitter regulation, tends to crystallize in flake crystals in the crystallization process, resulting in difficulty in processability and poor product quality. Faced with this challenge, in this work, the approach of spherulite growth induced by the polymeric additive was applied to form spherulites of L-tryptophan with effectively improved powder properties. In detail, L-tryptophan spherulites were fabricated through cooling crystallization under the effect of hydroxypropyl methylcellulose (HPMC). Then, the formation mechanism of L-tryptophan spherulites was analyzed based on the results of experiments and molecular simulations. Besides, the effect of supersaturation coupling additive concentration on the morphology of L-tryptophan spherulites was investigated. It turns out that the formation of L-tryptophan spherulites follows the classical non-crystallographic branching for spherulitic growth, in which HPMC preferentially adsorbs on the (001) face of L-tryptophan to introduce stress triggering the branching. Furthermore, the tap density of the prepared L-tryptophan spherulites was improved and nearly 4 times that of the L-tryptophan flake crystals, and the angle of repose of the L-tryptophan flake crystals was 46°, while the angle of repose of L-tryptophan spherulites was 29°, exhibiting the improved flowability for the post-processing. To sum up, this study provides a feasible method for the manufacture of L-tryptophan crystals with good product quality, as well as making efforts to reveal the mechanism of spherulitic growth, which may provide new insight into the structure design of organic crystals.
研究领域:
晶体工程
·
药学
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材料科学
·
高分子科学
·
物理化学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yanna Ding,Yanli Zhou,Cuilan Feng,Maotian Xu,Daosheng Liu
An in situ confinement polymerization technology was developed to construct nitrogen-doped carbon tubes (NCT) with a uniform distribution of silver nanoclusters (AgNCs). Specifically, polydopamine (PDA) was utilized as a good stabilizer and carbon precursor by using curcumin as a template to induce the self-polymerization of dopamine in the presence of Ag+. Then, the AgNC@NCT materials were formed by template removal and subsequent carbonization. The prepared AgNC@NCT materials exhibited excellent stability, catalytic activity, and electrochemical properties due to their high carbonization and silver content. The AgNC@NCT-modified electrode had strong catalytic oxidation activity, specific surface area, and charge transfer efficiency for the detection of d-tryptophan (d-Trp). The constructed electrochemical sensor demonstrated a good linear range (0.1–100 μM) with a low detection limit (0.009 μM) for d-Trp detection. The sensor also had excellent selectivity, high stability, and repeatability. More importantly, it could be used for the evaluation of d-Trp levels in human serum with high accuracy. This method provided a strategy for the detection of d-Trp in biological samples, which had a good application prospect in daily clinical tests.
研究领域:
材料科学
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分析化学
·
生物医学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Hongfang Zhao,Jianrong Wang,Hongxia Li,Xiaohui Niu,Hui Xu,Kunjie Wang
The enantioselective recognition and separation of racemic tryptophan are of significant importance in the fields of medicine, pharmaceutics, and biochemistry. However, conventional methods are costly, energy intensive, and environmentally unfriendly. In this case, l-Cys with amino (−NH2) and sulfhydryl (−SH) groups was chosen to modify β-CD with a chiral cavity to achieve self-assembly via hydrogen bonding, which not only serves as an electrochemical chiral sensor with enhanced chiral sites at the sensing interface but also achieves a higher enantioselectivity for d-tryptophan enantiomers through hydrogen bonding between the host and guest. Moreover, the d-tryptophan enantiomers were subsequently degraded under catalytic conditions simulating visible light, which was a new approach to introduce chiral properties in photocatalysis without organic synthesis. The use of inclusion-complex formation with β-CD combined with simple electrochemical technology and photocatalytic degradation techniques to efficiently and rapidly identify and degrade harmful d-type amino acid enantiomers offers new avenues, which would have great potential in future studies.
研究领域:
医学
·
生物化学
·
药学
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产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2024
·
IF 5.2
Shidong Zhou,Yuer Zhao,Xiaoyan Li,Qianli Ma,Hui Du,Xiaofang Lv,Zhilei Wu
The carbon capture and storage technology based on the hydrate method can effectively capture CO2 gas and reduce greenhouse gas emissions; however, the slow and random formation of hydrate has greatly hindered the commercial application of this technology. The effect of l-tryptophan (l-trp) on the kinetics of CO2 hydrate formation with graphene oxide (GO) was investigated. The results showed that the combination of l-trp and GO could effectively accelerate the hydrate formation rate, increase the gas consumption, and promote the formation of the CO2 hydrate. Compared with pure water, the optimal concentration of 0.200 wt % l-trp and 0.020 wt % GO can increase the cumulative gas consumption by 16.229%, shorten the induction time by 69.300%, and shorten the time of 90% hydrate formation by 54.895%. Kinetic experiments and morphological observations show that a low dose of GO could promote heat and mass transfer in the process of CO2 hydrate formation, but with the increase of concentration, GO particles will agglomerate, and the probability of hydrate nucleation will be greatly reduced. The strong wall-climbing effect of l-trp could promote hydrate growth against the reactor wall and gas–liquid exchange. This work provides an experimental basis for the commercialization of the hydrate technology.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Ting Zhang,Zhenqi Zhu,Yujing Bian,Xun Zhang,Lvzhu Cheng,Hao Qin,Bin Yang
Tryptophan, as an essential amino acid, can only be obtained through diet, and its production and processing are accompanied by different degrees of oxidation leading to poor absorption. Therefore, it is important to pay attention to the degree of oxidation of tryptophan promptly. Conventional tests can assess tryptophan oxidation levels by monitoring the amount of tryptophan, but most require complex pre-processing and time-consuming testing procedures. Based on this, we propose a rapid method to estimate the degree of tryptophan oxidation using terahertz spectroscopy. We confirmed the relationship between the terahertz characteristic absorption peaks of tryptophan and its content combined with X-ray diffraction and infrared spectroscopy. It was further found that the tryptophan content could be effectively determined based on the characteristic peak area. The findings of this study offer a novel and practical technique for quickly determining the level of tryptophan oxidation in food or dietary supplements.
研究领域:
食品科学
·
生物化学
·
光谱学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yi-xin Sun,Jia-hui He,Dan-dan Zhang,Yang Sheng,Defeng Xu,Rong Zhang,Mark Bradley
The rational design of electrochemical methods for chiral recognition is a focus of research in the detection fields of biomolecules and pharmaceuticals. In this work, electrochemical chiral interface based on chitosan (CS) and deoxyribonucleic acid (DNA) was constructed with self-assembly technology, and then characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), cyclic voltammetry, and electrochemical impedance spectroscopy. The FT-IR and SEM results showed the spatial structure of DNA and CS changed significantly and formed superhelix structure during the self-assembly process. Differential pulse voltammetry (DPV) results showed that the oxidation peak current ratio of D -Trp to L -Trp (I D /I L ) was observed to be 4.02 at 25 °C due to the synergistic effects of CS and DNA, obviously higher than that on any individual CS or DNA modified electrode. In addition, there was a linear relationship between the peak current and Trp enantiomer concentration in the range of 0.005–0.15 mM, and the detection limits of D-Trp and L-Trp were 1.33 and 1.67 µM, respectively. More importantly, the chiral interface can also be used to identify the percentage of D-Trp in non-racemic Trp enantiomers mixture solutions, showing its effectiveness and promising potential in practical applications.
研究领域:
生物化学
·
分析化学
·
电化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 46
·7703-7709
·
IF 4.776
Min Qiao,Xun Liu,Jin-Wei Song,Ting Yang,Ming-Li Chen,Jian-Hua Wang
Tryptophan (Trp) modified aminated mesoporous silica nanoparticles (AMSNs), shortened to Trp-AMSNs, are prepared via covalent binding. The obtained Trp-AMSNs exhibit a uniform size of ca. 83 nm, a mesopore diameter of ca. 2.6 nm, along with a pore volume of 0.439 cm3 g−1. It is demonstrated that Trp-AMSNs selectively adsorb ovalbumin (Ova) from complex biological matrices. At pH 5.0, 1.0 mg of Trp-AMSNs produces an adsorption efficiency of 96% for 100 mg L−1 Ova in 1.0 mL of solution. An adsorption capacity of 1240.3 mg g−1 is derived for Ova, which is much improved with respect to that of the native AMSNs. The retained Ova could be readily recovered by a sodium dodecyl sulfate (SDS) solution (0.5%, m/v), providing a recovery of 71.2%. Trp-AMSNs are further applied for the isolation of Ova from a protein mixture (with a molar ratio of ovalbumin/lysozyme of 1 : 10) and an egg-white sample. High-purity Ova is obtained, as demonstrated by SDS-PAGE assay results.
研究领域:
纳米技术
·
生物化学
·
材料科学
·
蛋白质工程
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DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2026
·
IF 2.4
Zishan Zhou,Hongquan Tao,Haocheng Yang,Ao Duan,Jiahui Yu,Yixin Chen,Yongyan Zhu,Quanhong Zhu
Favorable biocompatibility is essential to biomaterials, and natural amino acids are recognized as the promising building block of polymers due to their non-toxicity and tunable side chains. We prepared polymeric nanoparticles (NPs) using N-acryloyl-L-tryptophan monomer by precipitation polymerization, and modified with polyethylene glycol and folate (PEG-FA) to improve the solubility and target folate-receptors (FR) overexpressed tumor tissues. Serving as drug carriers for vinblastine (VBL), NPs-PEG-FA with about 212.4 nm had the drug loading of VBL of 6.65 ± 0.41% after co-incubating for 1 h and showed sustained-release in pH 7.4 PBS, in which 99.87 ± 1.00% of VBL was released from NPs-PEG-FA during 72 h. Furthermore, NPs-PEG-FA was more efficiently taken up by FR positive Hela cells compared with NPs-PEG, which signified folate could enhance the internalization of NPs-PEG-FA into FR over-expressed cells. And NPs-PEG-FA began to enter Hela cells in large quantities from 3 h onwards, meanwhile the released drug increased more quickly in the first 3 h, which indicated most of the drugs would be released after entering tumor cells. More importantly, NPs-PEG-FA had good biocompatibility to L929 mouse fibroblast cells and exhibited hemocompatibility via the assays of hemolysis, antithrombogenicity, coagulation activation and platelet activation. NPs-PEG-FA could serve as drug carriers for delivering drugs into FR positive tumor cells.
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DOI
高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2014
·Vol. 50
·期 11
·1065-1071
·
IF 0.66
Chao Mingyong,Ma Xinying
Herein, a poly( L -tryptophan) modified glassy carbon electrode (Ptry/GCE) for the determination of maltol is fabricated by electrochemical polymerisation. The electrochemical behaviour of maltol at the Ptry/GCE is studied by cyclic voltammetry (CV). The modified electrode shows excellent electrocatalytic activity towards the oxidation of maltol and the oxidation is a one-proton-one-electron process. In pH 8.0 phosphate buffer solution (PBS), the oxidation peak current of maltol shows a linear relationship with its concentration in the range from 9.00 × 10 −5 to 3.75 × 10 −3 mol L −1 with a correlation coefficient of 0.9972. The limit of detection is estimated to be 8.00 × 10 −6 mol L −1 . The novel method shows good selectivity, recovery, reproducibility and great convenience and has been satisfactorily demonstrated in real food sample analysis.
研究领域:
电分析化学
·
食品安全
·
传感器与生物传感器
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Tao Zhan,Bo Zhao,Zhen-Fan Zhou,Zhencheng Chen,Min Zhang,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Herein, we constructed an integrated system for L -tryptophan ( L- Trp) detection by assembling an electrochemical sensor and a microfluidic platform. Amongst, the sensor was printed using conductive polymer filaments and then chemically activated to expose porous nanostructure on the surface. A calibration curve was established between 31.26–1000.00 μmol/L and the limit of detection (LOD) was 10.41 μmol/L. The sensor exhibited satisfactory reproducibility, selectivity and stability. The recovery ranged between 99.06–102.09 % with relative standard deviations less than 5.21 %. The results show that L -Trp undergoes a two-proton, two-electron transfer in the anodic reaction. Once integrated into a printed microfluidic module from photosensitive resin, the sensor showed a wider detection range (15.00–70.00, 100–4000 μmol/L) with a lower LOD (5.00 μmol/L), which may be due to faster mass transfer in microfluidics. The consistency and validity were affirmed with ultra-visible spectrophotometer and HPLC. The integrated system allows point-of-testing owing to its small size, portability and low cost.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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