BIOMEDICINE & PHARMACOTHERAPY
·
2012
·
IF 2
Lan Qian,Di Xiang,Jing Zhang,Shunying Zhu,Jin Gao,Xia Wang,Jing Gao,Yang Zhang,Jiaqing Shen,Yan Yu,Wei Han,Mingyuan Wu
Cyclophosphamide (CY), targeting to fast dividing cells, results in bone marrow (BM) suppression, which is the most common side effect of cancer chemotherapy . Interleukin-1 receptor antagonist (IL-1Ra), activated by variety of chemotherapeutic drugs, is a natural inhibitor of interleukin-1 (IL-1) and blocks the functional IL-1 receptor signaling. Our previous studies showed the protective effect of recombinant murine IL-1Ra on hematopoiesis in mice after treatment with chemotherapeutic agent 5-fluorouracil. In this report, we demonstrate that the pretreatment use of recombinant human IL-1Ra (rhIL-1Ra) significantly alleviated chemotherapy-induced peripheral blood injury in mice, and reduced the incidence and severity of neutropenia in beagle dogs. Moreover, acute lethal toxicity in single and repeated CY treatment was markedly reduced by rhIL-1Ra administration. The chemoprotective role of rhIL-1Ra is attributed to the attenuated BM damage, accelerated recovery of BM cells , and enhanced survival of hematopoietic progenitor cells which expressed high level of aldehyde dehydrogenase and IL-1 receptor type I. Thus, our data strongly suggest that the prophylactic use of exogenous rhIL-1Ra renders BM primitive hematopoietic cells resistant to chemotherapy, which provides novel strategies to prevent BM suppression in clinical settings.
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2012
·
IF 4.98
Zhixing He,Hailong Xiao,Lu Tang,Hang Min,Zhenmei Lu
HD-1, a stable microbial consortium capable of mineralizing di- n -butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25–30 °C and pH 8.0–9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography–mass spectrometry (GC–MS) during the degradation process. The HD-1 consortium also utilized phenol, o -dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Yuxia Lv,Yaliang Chen,Ziqiang Shao,Renxu Zhang,Libin Zhao
Homogeneous tritylation of cellulose in 1-allyl-3-methylimidazolium chloride (AmimCl) ionic liquid with triphenylmethy chloride as regents, pyridine or 1-butylimidazole (BIM) as base was investigated, and subsequent acetylation of the 6- O -functionalized products was further studied. The structure of products was analyzed by FTIR and 13 C NMR spectroscopy and base influences on the structure were discussed as well. The solution with pyridine as base underwent heterogeneous–homogeneous–heterogeneous process and the obtained trityl cellulose (TC) had organized structure with trityl group located completely at C-6 position of cellulose with maximum DS trityl of nearly 1. In the case of BIM as base, the solution was homogeneous for the whole reaction, but the highest DS trityl was about 0.22, with trityl group located not only at position 6 but also partially at position 2. Subsequent acetylation of the TC led to products with a preferred functionalization of the unprotected secondary OH-groups.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 132
·期 34
·
IF 1.768
Xiangyan Liu,Deng Hu,Meng Li,Jianming Zhang,Zhigao Zhu,Gaofeng Zeng,Yanfeng Zhang,Yuhan Sun
To improve the pervaporation performance of Silicalite-1/PDMS composite membrane by adding a small amount of Silicalite-1 zeolite, novel Silicalite-1/PDMS surface sieving membranes (SSMs) were prepared by attaching Silicalite-1 particles on the PDMS membrane surface. The obtained membranes and traditional mixed-matrix membranes (MMMs) were characterized by SEM, XRD, TGA, FT-IR, and pervaporation separation of ethanol–water mixture. Effects of Silicalite-1 particles content, feed temperatures, and feed compositions on the separation performance were discussed. From the cross-section view SEM images of SSMs, a two-layer structure was observed. The thickness of the Silicalite-1 layer was about 300 nm to 2 μm. The TGA analysis indicates that the zeolite concentration in 3 wt % SSM is lower than 10 wt % MMMs. In the ethanol/water pervaporation experiment, the separation factor of Silicalite-1/PDMS SSMs increased considerably compared with pure PDMS membrane. When the suspensions concentrations of Silicalite-1 particles reached 3 wt %, the separation factor was about 217% increase over pure PDMS membrane and 52.9% increase over 10 wt % Silicalite-1/PDMS MMMs. As the ethanol concentration in the feed increases, the separation factor of SSMs increases, whereas permeation flux decreases. At the same time, with increasing operating temperature, the permeation flux of SSMs increased. The stability of SSMs at high temperature is better than the traditional MMMs. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42460.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2014
·Vol. 29
·期 5
·1090-1097
·
IF 0.422
Tang Yimei,Hu Xiaoling,Guan Ping,Li Xiaoqian,Tian Tian
Several 1-vinyl-3-alkylimidazolium halogens [VRIM]X, which are functional materials with ethylenic bonds, were synthesized using the microwave-assisted synthesis method. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy were carried out to analyze the resultant structures. The electrochemical properties and solubility of [VRIM]Br were investigated and discussed in detail. The temperature dependence of pure [VRIM]Br over a wide temperature range of 298.15–323.15 K fitted the Arrhenius equation well. At certain low concentrations, the electrical conductivity of the [VRIM]Br solution significantly increased with increasing solution concentration. The electrical conductivities of the [VRIM]Br observed in water, methanol, and ethanol showed the trend σ
water > σ
methanol > σ
ethanol . Conductometry showed that the critical micelle concentrations of the bromines in water, methanol, and ethanol were 6.8–6.9 × 10 −6 , 1.4–1.5 × 10 −5 , and 1.9–2.0×10 −5 mol·L −1 , respectively; these results indicate that [VRIM]Br is an excellent surfactant. The solubility of [VRIM]X in common solvents was determined at 293.15 K, and results indicated that a decrease in solubility could be observed with decreasing dielectric constant of the solvent, elongation of the alkyl chain of the cation, and increasing anion size. Solubility parameters were also determined according to the Hildebrand-Scoff equation.
✓ 已验证
产品关联
DOI
高影响因子
BRITISH JOURNAL OF PHARMACOLOGY
·
2014
·Vol. 171
·期 23
·5252-5264
·
IF 4.99
Jing Han,Lidan Sun,Xun Huang,Zheng Li,Chenyu Zhang,Hai Qian,Wenlong Huang
Background and Purpose The short biological half-life limits the therapeutic use of glucagon-like peptide-1 (GLP-1) and chemical modification to improve the interaction of peptides with serum albumin represents an effective strategy to develop long-acting peptide analogues. Coumarin, a natural product, is known to bind tightly to plasma proteins and possesses many biological activities. Therefore, we designed and synthesized a series of coumarin-modified GLP-1 derivatives, hypothesizing that conjugation with coumarin would retain the therapeutic effects and prolong the biological half-life of the conjugates. Experimental Approach Four cysteine-modified GLP-1 analogues (1–4) were prepared using Gly 8 -GLP-1(7–36)-NH 2 peptide as a starting point. These analogues were conjugated with two coumarin maleimides to yield eight compounds (conjugates 6–13) for testing. Activation of human GLP-1 receptors, stability to enzymic inactivation in plasma and binding to human albumin were assessed in vitro . In vivo , effects on oral glucose tolerance tests (OGTT) in rats and on blood glucose levels in db/db mice were studied. Key Results Most conjugates showed well preserved receptor activation efficacy, enhanced albumin-binding properties and improved in vitro plasma stability and conjugate 7 was selected to undergo further assessment. In rats, conjugate 7 had a longer circulating t 1/2 than exendin-4 or liraglutide. A prolonged antidiabetic effect of conjugate 7 was observed after OGTT in rats and a prolonged hypoglycaemic effect in db/db mice. Conclusions and Implications Cysteine-specific coumarin conjugation with GLP-1 offers a useful approach to the development of long-acting incretin-based antidiabetic agents. Conjugate 7 is a promising long-lasting GLP-1 derivative deserving further investigation.
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY
·
2013
·
IF 4.017
Meijuan Tu,Siyuan Sun,Kai Wang,Xueying Peng,Ruihan Wang,Liping Li,Su Zeng,Hui Zhou,Huidi Jiang
Monocrotaline (MCT) is a kind of toxic retronecine-type pyrrolizidine alkaloids (PAs) from plants of Crotalaria , which can be bio-activated by cytochrome P450 (CYP) enzymes in liver and then induce hepatotoxicity. Since CYPs are localized in the endoplasmic reticulum , the influx of MCT to the liver is the key step for its hepatotoxicity. The objective of the present study was to investigate the role of organic cation transporter 1 (OCT1), a transporter mainly expressed in liver, in the uptake of MCT and in hepatotoxicity induced by MCT. The results revealed that MCT markedly inhibited the uptake of 1-methyl-4-phenylpyridinium (MPP + ), an OCT1 substrate, in Madin–Darby canine kidney (MDCK) cells stably expressing human OCT1 (MDCK-hOCT1) with the IC 50 of 5.52 ± 0.56 μM. The uptake of MCT was significantly higher in MDCK-hOCT1 cells than in MDCK-mock cells, and MCT uptake in MDCK-hOCT1 cells followed Michaelis–Menten kinetics with the K m and V max values of 25.0 ± 6.7 μM and 266 ± 64 pmol/mg protein/min, respectively. Moreover, the OCT1 inhibitors, such as quinidine , d -tetrahydropalmatine ( d -THP), obviously inhibited the uptake of MCT in MDCK-hOCT1 cells and isolated rat primary hepatocytes, and attenuated the viability reduction and LDH release of the primary cultured rat hepatocytes caused by MCT. In conclusion, OCT1 mediates the hepatic uptake of MCT and may play an important role in MCT induced-hepatotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2013
·Vol. 29
·期 4
·730-734
·
IF 0.735
Zhang Lei,Zhang Shi-tao,Zhang Xiao-ping,Sun Jing,Wang Yong-sen,Liu Yue-long,Xue Miao-miao,Wang Zhi,Xing Shu,Ma Jun-feng,Li Wan-nan,Fu Xue-qi
The protein tyrosine phosphatases(PTPs) comprise a family of enzymes that specifically dephosphorylate tyrosyl residues. Among them, SHP-1 has been regarded as one of the best validated intracellular tyrosine phosphatases. Downregulation of SHP-1 has shown remarkable efficacy in improving insulin sensitivity in vivo in insulin signaling pathway. In this study, we found the role of Candesartan cilexetil targeting at SHP-1. The results indicate that Candesartan cilexetil was a competitive inhibitor to SHP-1(IC 50 =85.6 μmol/L and K
i =24 μmol/L). We also found that Candesartan cilexetil was more sensitive towards SHP-1 compared with other PTPs. Through the consequence of Western blotting, it showed that Candesartan cilexetil can strengthen the level of tyrosine phosphorylation of several key cellular proteins[such as insulin receptor(IR), insulin receptor substrate(IRS) and ERK]_in insulin signaling pathway in HepG 2 cells and improve the insulin sensitivity through inhibiting the protein phosphorylation of SHP-1. These findings showed that Candesartan cilexetil might be an important inhibitor of SHP-1 and had a great application potential in the treatment of diabetes through inhibiting the level of SHP-1 in insulin signaling pathway.
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2013
·
IF 4.576
Jing Han,Xun Huang,Lidan Sun,Zheng Li,Hai Qian,Wenlong Huang
A series of fatty chain conjugates of glucagon-like peptide-1(GLP-1) were designed and synthesized. First, eleven cysteine modified peptides ( 1 – 11 ) were prepared using Gly 8 -GLP-1(7-36)-NH 2 peptide as a starting point. Peptides 1 , 6 , 9 , and 11 which showed comparable GLP-1 receptor activate potency and glucose-lowering effect in vivo with Gly 8 -GLP-1(7-36)-NH 2 were selected for second step modifications to yield conjugates 12 – 23 . All conjugates retained significant GLP-1 receptor activate potency and more importantly exerted enhanced albumin-binding properties and in vitro plasma stability. The protracted antidiabetic effects of the most stable compound 14 were further confirmed by both multiple intraperitoneal glucose tolerance test and hypoglycemic efficacies test in vivo. Furthermore, once daily injection of compound 14 to db/db mice achieved long-term beneficial effects on HbA1c lowering and glucose tolerance. Our results suggest that compound 14 is a promising type 2 antidiabetic agent deserving further investigation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2014
·
IF 3.679
Yuming Cui,Na Liu,Yifen Xia,Jiangang Lv,Sujuan Zheng,Nianhua Xue,Luming Peng,Xuefeng Guo,Weiping Ding
1-Butene, abundant but inexpensive in industry, can be used to produce more valuable propene and ethene through the well-known metathesis reaction. In terms of catalyst system of molybdena, controlling the interaction between the molybdena and the support is quite crucial. A special support, i.e., well-shaped one-dimensional γ-Al 2 O 3 with uniform diameters of ∼10 nm and lengths of ∼100 nm, has been synthesized via oleylamine-assisted hydrothermal method. The molybdenum oxides are well controlled in sizes of moderate aggregation suitable for 1-butene metathesis with a fairly high rate of propene production (38.9 × 10 −9 mol m −2 s −1 , 30 mol% yield in a single pass) under mild conditions. The status of MoO x species in the catalyst has been investigated by solid state 29 Si MAS NMR, Raman, UV–vis, H 2 -TPR and NH 3 -TPD techniques. The catalyst, highly stable after twelve times of regeneration, is important for practical utilization for the metathesis reactions.
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS LETTERS
·
2016
·
IF 2.437
Shannan Chang,Cuiping Huang,Huimin Zhang,Aimin Chang
(1− x )BaSn 0.96 Sb 0.04 O 3 – x CoFe 0.5 Co 1.5 O 4 ( x =0.1, 0.3, 0.5, 0.7 and 0.9) composite ceramics had been fabricated through conventional solid-state method. The X-ray diffraction analysis indicated that the composite ceramics consisted of two phases: a perovskite BaSn 0.96 Sb 0.04 O 3 phase and a cubic spinel CoFe 0.5 Co 1.5 O 4 phase. The composite ceramics were more dense and lower porosity with increasing the CoFe 0.5 Co 1.5 O 4 content by SEM. The obtained ρ 25 and B 25/50 constants and activation energy of the composite ceramics were in the range of 1.61×10 5 –1.06×10 6 Ω cm, 1048–8458 K and 0.09–0.729 eV, respectively. In particular, their relationship between R and T which differed from normal NTC displayed a good linear characteristic at 0–200 °C. It could be used expediently in digital instrument and precision measurement.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2017
·
IF 3.339
Xiaobing Jiang,Yameng Xu,Yi Li,Kun Zhang,Lei Liu,Hailei Wang,Jinhe Tian,Hao Ying,Lei Shi,Tao Yu
The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Escherichia coli isolated from retail meats. Among the 179 E. coli isolates tested, the minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to 64 μg/mL. PCR assays indicated that QAC-resistance genes sugE(c) , ydgE / ydgF , mdfA , emrE and qacE Δ1 were commonly present (40.2%–88.3%) in these isolates, but qacE , qacF , qacH and sugE(p) were less prevalent (2.2%–28.5%). Seven different gene cassette arrangements were identified in 31 intI1 -positive isolates. Three types of qacH-sul3 -associated non-classic integrons were observed in four isolates: dfrA12 - orfF - aadA2-cmlA1-aadA1-qacH- IS 440-sul3 , aadA2-cmlA1-aadA1-qacH- IS 440-sul3 and dfrA1-aadA1-qacH- IS 440-sul3 . Non-classic class 1 integrons were located on plasmids of 100–150 kb in these four isolates. Our results demonstrated that the qacH -associated integrons located on 100 kb plasmids in two isolates could be transferred to an E. coli recipient, indicating the co-existence and co-dissemination of disinfectant and antimicrobial resistance genes among bacterial species.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）