MATERIALS RESEARCH BULLETIN
·
2025
·
IF 5.7
Lanfei Wei,Jiaquan Liu,Qiuman Zhou,Shaolong Liu,Zhifeng Su,Yurong Yan
Bismuth trioxide (Bi 2 O 3 ) acts as an ideal material for supercapacitors because of its high theoretical specific capacity, but its crystal form and compact accumulation limit its specific capacitance below its theoretical value of 1370 F g -1 . In this study, a specific kapok fiber@chitosan-derived carbon (KCC) doping inside β/α hybrid-crystal Bi 2 O 3 (KCC-Bi 2 O 3 ) was constructed by KCC assisting to enhance the contact area between Bi-MOF and air during calcination. A specific capacitance of 1135 F g -1 at 1 A g -1 was obtained for KCC-Bi 2 O 3 , which matched 82.3 % of its theoretical value. An asymmetric capacitor, with KCC-Bi 2 O 3 as a negative electrode and NiCo-LDH as a positive electrode, exhibits a potential window of 1.6 V and an energy density of 17.3 Wh kg -1 at 800 W kg -1 . The result demonstrates that incorporating a KCC carbon source can significantly enhance the capacitance of mixed crystal form Bi 2 O 3 , highlighting its potential in supercapacitor applications.
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ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Cheng Li,Qinghui Chen,Jinqiao Rong,Houde He,Yu Lu,Yuxia Liu,Zhongfu Wang
As the main active glycoprotein of egg white, the biological functions of chicken ovomucin α- and β-subunit are closely related to the structure of glycans. However, the exact composition and structure of the subunit glycans are still unknown. We obtained highly pure chicken ovomucin α-subunit and β-subunit protein bands by the strategy combined with two-step isoelectric precipitation and SDS-PAGE gel electrophoresis. The ammonia-catalyzed one-pot procedure was then used to release and capture α-and β-subunit protein glycans with 1-phenyl- 3-Methyl-5-pyrazolone (PMP). The N / O -glycans of bis-PMP derivatives were purified and analyzed by LC-MS. More importantly, an effective dual modification was performed to accurately quantify neutral and sialylated O -glycans through methylamidation of sialic acid residues and simultaneously through carbonyl condensation reactions of reducing ends with PMP. We first showed that the α-subunit protein has only N -glycosylation modification, and the β-subunit only O -glycosylation, a total of 22 N -glycans and 20 O -glycans were identified in the α- and β-subunit, respectively. In addition, the complex N -glycan (47 %) and the sialylated O -glycan (77 %) are each major types of the above subunits. Such findings in this study provide a basis for studying the functional and biological activities of chicken ovomucin glycans.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhe-Ying Hu,Huan-Yu Sui,Qi-Feng Zhong,Lu Hu,Jie-Hua Shi,Shao-Liang Jiang,Liang Han
Dacomitinib is a highly selective second-generation tyrosine kinase inhibitor that can irreversibly bind to tyrosine kinase and is mainly used in the treatment of lung cancer. The binding characteristics of dacomitinib with human α 1-acid glycoprotein (HAG) were analyzed by multispectral and computational simulation techniques. The fluorescence spectra showed that dacomitinib can quench the fluorescence of HAG by forming the HAG-dacomitinib complex with a molar ratio of 1:1 (static quenching). At the temperature similar to that of the human body, the affinity of dacomitinib to HAG (8.95 × 10 6 M −1 ) was much greater than that to BSA (3.39 × 10 4 M −1 ), indicating that dacomitinib will give priority to binding onto HAG. Thermodynamics parameters analysis and driving force competition experiments showed that hydrogen bonding and hydrophobic forces were the major sources for keeping the complex of HAG-dacomitinib stable. The experimental outcomes also showed that the binding of dacomitinib can lead to the loosening of the skeleton structure of HAG, which led to a slight change in the secondary structure , and also reduces the hydrophobicity of the microenvironment of Trp and Tyr residues. The binding sites of dacomitinib on HAG and the contribution of key amino acid residues to the binding reaction were determined by molecular docking and molecular dynamics (MD) simulation. In addition, it was found that there was a synergistic effect between dacomitinib and Mg 2+ and Co 2+ ions. Mg 2+ and Co 2+ could increase the K b of dacomitinib to HAG and prolong the half-life of dacomitinib.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yanping Xia,Feng Shi,Ruixin Liu,Haibing Zhu,Kai Liu,Chuanli Ren,Juan Li,Zhanjun Yang
Designing a metal–organic framework (MOF)-derived nanozyme with highly dispersed active sites and high catalytic activity as well as robust structure for colorimetric biosensing of diverse biomolecules remains a substantial challenge. Here, an MOF-derived highly dispersed and pure α-cobalt confined in a nitrogen-doped carbon nanofiber (α-Co@NCNF) nanozyme with superior glucose oxidase (GOD)- and peroxidase (POD)-like activities was constructed for colorimetric assay of multiple biomolecules. Specifically, the α-Co@NCNF nanozyme was synthesized, utilizing in situ electrospinning Co-MOFs into polyacrylonitrile nanofiber (PAN) followed by a pyrolysis process. Taking advantage of the in situ electrospinning strategy, the α-Co nanoparticles were confined in continuous porous NCNF to restrict the growth and prevent the aggregation and oxidation during the pyrolysis process. The resulting special structure considerably improved the enzyme-like performance. A series of experiments validate that the enzyme-like activity of the α-Co@NCNF nanozyme was superior to that of Co@CoO@NCNF (derivatives from Co-MOFs grown on the surface of PAN nanofiber) and nature enzymes. Furthermore, α-Co@NCNF nanozyme-based colorimetric biosensing was developed for monitoring glucose, hydrogen peroxide (H2O2), and glutathione (GSH) and the corresponding linear ranges are 0.1–50 and 50–900 μM and 5–55 and 0.1–20 μM accompanied by the corresponding low detection of 0.03, 1.66, and 0.03 μM. The proposed method for the construction of α-Co@NCNF nanozyme with dual enzyme-like properties provides a new insight for designing novel nanozymes and has prospects for application in colorimetric biosensing.
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Foods
·
2024
·Vol. 13
·期 7
·1070
·
IF 4.7
Jin Liu,Li Ma,Senwen Deng,Xinzhi Chen,Qi Li,Aiqing Xu,Ting Tong,Shuhua Tan,Mingkang Wang,Jiangtao Cai,Haihua Wang
During the production process of refined betel nuts in China, a large amount of processing by-product, betel nut waste seeds, is generated. Betel nut waste seeds are rich in bioactive elements, but they have not been effectively utilized yet. In this study, an ultrasonic-assisted deep eutectic solvent method (DES) was used to selectively extract α-glucosidase inhibitors from waste seeds. Compared with traditional extraction solvents such as water and ethanol, the extraction efficiency of specific DESs is higher, and the content of alkaloids in the extracts is lower. However, it should be noted that some pure DESs exhibit inhibitory activity towards α-glucosidase. DESs, based on choline chloride/urea, were selected due to the high extraction efficiency of α-glucosidase inhibitors and their low alkaloid content as well as low inhibitory activity. The optimal extraction conditions were determined using single-factor experiments as follows: 30% (v/v) water content, a choline chloride/urea ratio of 5:3, a solid–liquid ratio of 1:10, extraction temperature of 40 °C, and a duration of 30 min. Through recovery experiments, it was found that the DES can be reused four times under these conditions, maintaining an inhibition rate comparable to alcohol extraction methods. The IC50value of the extract was measured at 0.0066 mg/mL, superior to acarbose. In summary, this research has successfully developed an efficient and selective method for extracting α-glucosidase inhibitors from betel nut waste seeds, thereby presenting a promising avenue for future applications.
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Food Bioscience
·
2024
·
IF 4.8
Jiaxuan Wang,Cuiping Pang,Zhonglin Tu,Jianghua Li,Guocheng Du,Guoqiang Zhang
L-Alanyl- l -Glutamine (Ala-Gln) is a kind of functional dipeptide , which is widely used in food and health care. Ala-Gln can be synthesized from l -glutamine and l -alanine methyl ester hydrochloride by α-Amino acid ester acyltransferase (AET). Still, the low catalytic efficiency and conversion rate of AET are key limiting factors in Ala-Gln production. In this study, the mutant library of AET were built based on structural model and semi-rational design. To facilitate the screening of AET mutants, the double enzymes coupled screening method was constructed and optimized. The amino acid residues I115, D203, F211, D300, A298, and R334 were selected as candidate mutation sites by alanine scanning and virtual saturation mutation and the catalytic activity of the optimal mutation A298C increased by 23.7%. Molecular dynamics simulation and molecular docking analysis suggested that the increased flexibility of the catalytic pocket in AET (A298C) and the formation of additional hydrogen bonds with substrate contributed to the improvement of enzyme activity .
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Jun-Hui Du,Dong-Mei Yang,Tong-Tong Guan,Jie Wang,Yi-Long Ma,Zhao-Jun Wei
Osmanthus fragrans seeds (OFSs) abundant in protein are valuable resources of bioactive peptides. To elucidate the potential hypoglycemic peptides released during the digestion, OFSs protein was extracted and digested in vitro . The effects of the digestion step and molecular weight on the α-glucosidase inhibitory ability of the digested protein were evaluated, and the mechanism and structure of the main effective peptides in the digesta were studied. The results indicated that the digesta after the small intestine digestion displayed higher inhibition (58.5 %), from which the fraction below 3 kDa was more effective (67.34 %). Among the seven sub-fractions (F1∼F7) below 3 kDa, F1 obtained the highest α-glucosidase inhibitory ability (60.69 %). Three peptides (ERFQ, VHHK, and EKFMKE) were identified from F1, and their efficacy was verified by corresponding synthetic peptides, showing the α-glucosidase inhibitory rates of 79.1 % ± 2.24 %, 65.67 % ± 1.97 % and 61.94 % ± 1.97 %, respectively. According to the molecular docking, these peptides inhibited the α-glucosidase by forming secondary forces (e.g. hydrophobic interaction) with the key amino acids (e.g. D327, D443, Y299, and F575) of the enzyme, with docking scores ranging from −151.038 to −171.907. This study demonstrated OFSs could release α-glucosidase inhibitory peptides during digestion and may serve as functional ingredients to control diabetes.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Guangli Li,Yafeng Zhang,Yonghui Xia,Tianyu Wang,Yuan Jin
Excessive consumption of Sunset Yellow (SY) and Tartrazine (Tz) might cause adverse effects on human health. Hence, it is essential to develop a rapid and reliable technique for simultaneous determination of SY and Tz. Herein, an innovative voltammetric sensor was constructed for simultaneous determination of SY and Tz using multiwalled carbon nanotubes decorated dandelion-like α-MnO 2 microspheres (MnO 2 /MWCNTs). The physiochemical properties of MnO 2 /MWCNTs were studied by various microscopic, spectroscopic, and electrochemical techniques. Various parameters, including accumulation time, accumulation potential, loading volume, buffer pH, and scanning rate, were systematically explored in order to pursuit the higher peak current response. The MnO 2 /MWCNT effectively enlarged the electroactive surface area and expedited the electron transfer. As a result, the MnO 2 /MWCNT decorated glassy carbon electrode (GCE) displayed superior sensing performance for the electrooxidation of SY and Tz over a wide concentration range (0.06–10 μM), owing to the synergistic effect from dandelion-like α-MnO 2 microspheres and highly conductive MWCNTs. The detection limits were 0.005 and 0.01 μM for SY and Tz, respectively. In addition, the MnO 2 /MWCNT/GCE demonstrated strong anti-interfering capacity against potential interfering species, and highly reproducible electrode manufacture. The MnO 2 /MWCNT/GCE was successfully employed for the simultaneous and sensitive determination of SY and Tz in carbonate beverage with good recovery.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Ruijie Liu,Jeroen Kool,Jingyi Jian,Jincai Wang,Xianglong Zhao,Zhengjin Jiang,Tingting Zhang
In this study, a novel at-line nanofractionation screening platform was successfully developed for the rapid screening and identification of α-glucosidase inhibitors from natural products. A time-course bioassay based on high density well-plates was performed in parallel with high resolution mass spectrometry (MS), providing a straightforward and rapid procedure to simultaneously obtain chemical and biological information of active compounds. Through multiple nanofractionations into the same well-plate and comparisons of the orthogonal separation results of hydrophilic interaction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC), the α-glucosidase inhibitors can be accurately identified from co-eluates. The screening platform was comprehensively evaluated and validated, and was applied to the screenings of green tea polyphenols and Ginkgo folium flavonoids. After accurate peak shape and retention time matching between the bioactivity chromatograms and MS chromatograms, ten α-glucosidase inhibitors were successfully screened out and identified. The proposed screening method is rapid, effective and can avoid ignoring low abundant/active inhibitors.
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Marine Drugs
·
2024
·Vol. 22
·期 9
·390
·
IF 4.9
Jinpeng Yu,Junjie Xie,Yuting Ma,Pengcheng Wei,Panpan Zhang,Zepei Tang,Xiaopeng Zhu,Dongting Zhangsun,Sulan Luo
α-Conotoxins are disulfide-rich peptides obtained from the venom of cone snails, which are considered potential molecular probes and drug leads for nAChR-related disorders. However, low specificity towards different nAChR subtypes restricts the further application of many α-conotoxins. In this work, a series of loop1 amino acid-substituted mutants of α-conotoxin RegIIA were synthesized, whose potency and selectivity were evaluated by an electrophysiological approach. The results showed that loop1 alanine scanning mutants [H5A]RegIIA and [P6A]RegIIA blocked rα7 nAChR with IC50s of 446 nM and 459 nM, respectively, while their inhibition against rα3β2 and rα3β4 subtypes was negligible, indicating the importance of the fifth and sixth amino acid residues for RegIIA’s potency and selectivity. Then, second-generation mutants were designed and synthesized, among which the analogues [H5V]RegIIA and [H5S]RegIIA showed significantly improved selectivity and comparable potency towards rα7 nAChR compared with the native RegIIA. Overall, these findings provide deep insights into the structure–activity relationship of RegIIA, as well as revealing a unique perspective for the further modification and optimization of α-conotoxins and other active peptides.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jiaqing Li,Donglin Li,Zhaoyang Zhang,Chang Yu,Dan Sun,Ziyao Mo,Jiayin Wang,Mmby Mohamed,Hong You,Hu Wan,Jianhong Li,Shun He
In this study, an α-amylase-responsive controlled-release formulation was developed by capping polydopamine onto β-cyclodextrin-modified abamectin-loaded hollow mesoporous silica nanoparticles. The prepared Aba@HMS@CD@PDA were subjected to characterization using various analytical techniques. The findings revealed that Aba@HMS@CD@PDA, featuring a loading rate of 18.8 wt %, displayed noteworthy release behavior of abamectin in the presence of α-amylase. In comparison to abamectin EC, Aba@HMS@CD@PDA displayed a significantly foliar affinity and improved rainfastness on lotus leaves. The results of field trail demonstrated a significantly higher control efficacy against Spodoptera litura Fabricius compared to abamectin EC at all concentrations after 7, 14, and 21 days of spaying, showcasing the remarkable persistence of Aba@HMS@CD@PDA. These results underscore the potential of Aba@HMS@CD@PDA as a novel and persistently effective strategy for sustainable on-demand crop protection. The application of nanopesticides can enhance the effectiveness and efficiency of pesticide utilization, contributing to more sustainable agricultural practices.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Chenlei Ma,Honglei Bao,Beibei Zhu,Junchao Zhu,Songlin Chen,Huawei Lv,Chu Chu,Shengqiang Tong
Micro-fraction bioactivity profiling and high speed countercurrent chromatography were performed for screening and isolation of components with α -glucosidase inhibitory activity from Eriobotrya japonica (Thunb.) leaves. Three sesquiterpene glycosides and a number of known pentacyclic triterpene acids, including a new compound, were successfully screened. Sesquiterpene glycosides were found to have α -glucosidase inhibitory activity for the first time. An efficient strategy for preparative separation of the three sesquiterpene glycosides from E. japonica leaves by column chromatography combined with two-step high speed countercurrent chromatographic separation was established. Two biphasic solvent systems, including n-hexane-ethyl acetate-methanol-water (5:95:5:95, v/v) and ethyl acetate-ethanol-water (100:20:80, v/v), were selected. In the first countercurrent chromatographic separation, 17.50 mg of sesquiterpene glycoside 1 with 92.9% purity and 15.11 mg of sesquiterpene glycoside 3 with 94.4% purity were isolated from 80.10 mg of partially purified fraction I, and in the second separation, 4.42 mg of new sesquiterpene glycoside 2 with 94.9% purity were isolated. Each screened compound was evaluated by α -glucosidase inhibition assay, and results showed that the new sesquiterpene glycoside 2 had high inhibitory activity with IC 50 = 7.83±0.01 μ M.
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危险品化学品经营许可证（带存储）