CELLULOSE
·
2025
·
IF 4.8
Wan Weimin,Wang Yuying,Li Ziqi,Wang Xi,Luo Jun
In recent years, cellulose nanocrystals (CNCs) have been used to deliver drugs, showing great potential in exploring new methods for cancer chemotherapy. Nevertheless, insolubility, low stability, and bioavailability of the potential bioactive compounds obstruct the development of bioactive formulations. Here, we constructed a pH-responsive nanocarrier that can actively target hepatocellular carcinoma cells (HCC) to deliver insoluble drug curcumin (Cur). Firstly, the polyethyleneimine (PEI) was coated onto the surface of CNCs (CNCs@PEI), and then the 3-carboxyphenylboronic acid (BA) was grafted with the coated PEI (CNCs@PEI-BA). After that, Cur formed a borate structure with BA to load Cur (CNCs@PEI-BA-Cur) showed a high drug loading capacity of 15.74%. CNCs@PEI-BA-Cur actively target HCC, enter into lysosomes, and release Cur in response to the low pH value environment. Importantly, CNCs@PEI-BA-Cur released 10.7-folds of Cur in acidic environments (pH = 5.4) more than in neutral environments (pH = 7.2). Furthermore, the nanocarrier showed efficient active targeting of HCC and increased intracellular Cur concentrations, which allowed for the effective killing of HCC in 2D cell and 3D hepatoma cell microsphere models in vitro. In conclusion, this targeted and pH-responsive nanocarrier was expected to enhance the therapeutic of insoluble drugs for HCC.
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ISME Journal
·
2025
·
IF 10
Yan Baofei,Zheng Xian,Lu Danya,Li Ting,Chen Xi,Shao Zhitao,Fu Tingming
Microbiota-associated factors are increasingly recognized as significant contributors to the progression of ulcerative colitis, and microbial modulation has emerged as an effective therapy for this condition. The herbal compound silibinin has demonstrated properties that modulate gut microbiota. Herein, we investigated the response of gut microbiota to silibinin in ameliorating colitis, using a mouse model of colitis coupled with antibiotic exposure. Results indicated that antibiotic pretreatment negated the benefits of silibinin in mice with colitis. Furthermore, fecal microbiota transplantation involving silibinin-modulated gut microbiota further substantiated the gut microbiota-dependent effects of silibinin. Within the metabolic profiles of silibinin-regulated microbiota, we identified that Alistipes-associated (R)-2,3-dihydroxy-isovalerate exhibited the most pronounced anti-inflammatory effects in vitro and demonstrated protective effects against colitis. Moreover, (R)-2,3-dihydroxy-isovalerate reinstated the protective effects of silibinin in mice with colitis under antibiotic exposure. These effects were primarily mediated via the targeting of the colonic GABA transporter 3 by (R)-2,3-dihydroxy-isovalerate. We further revealed that the retinoic acid receptor β and the retinoid-related orphan nuclear receptor γt may mediate the impact of silibinin-derived microbiota and (R)-2,3-dihydroxy-isovalerate on colitis. Additionally, the knockdown of colonic GABA transporter 3 diminished the impact of silibinin on the GABA transporter 3/retinoic acid receptor β/retinoid-related orphan nuclear receptor γt axis and colitis. Our findings highlight that (R)-2,3-dihydroxy-isovalerate, enriched from microbiota derived from silibinin, can target the GABA transporter 3/retinoic acid receptor β/retinoid-related orphan nuclear receptor γt axis, which is essential for anti-colitis properties of silibinin-regulated microbiota.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Liang Cao,Jiajia Wang,Feifan Hu,Huihui Liu,Jian Guo,Haoran Yu,Mingyuan Yan,Ji Zhang,Feifei Wang,Yiping Wang,Shan-Tao Zhang
In this work, based on the lanthanide rare earth Sm3+ doped 0.2Pb(Mg1/3Nb2/3)O3-(0.8-x)PbZrO3-xPbTiO3:0.015Sm ceramics with a rhombohedral-tetragonal morphotropic phase boundary (MPB) around x = 0.40, we gradually introduced alkaline earth Ba2+ and Sr2+ at the A-site to substitute Pb2+, namely, 0.2A(Mg1/3Nb2/3)O3-0.4AZrO3-0.4ATiO3:0.015Sm (A = Pb0.99-yBa0.01Sry) and conducted a systematic study on their phase structure, domain configuration, and electrical properties. The results indicate that without alkaline earth substituting, the MPB composition shows peak electrical property with piezoelectric coefficient d33 = 477 pC/N and electromechanical coupling factor kp = 0.59, while an appropriate amount of alkaline earth substituting changes the MPB from rhombohedral–tetragonal coexisting phases to rhombohedral–monoclinic–tetragonal coexisting phases, leading to a significant enhancement in electrical properties with d33 = 687 pC/N and kp = 0.63 around y = 0.03 for comparison. This work classifies the mechanism for alkaline earth substituting enhanced piezoelectric performance and may provide a guide for the development of high-performance lead-based relaxor ferroelectrics.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Feng Zhang,Mengke Wang,Lili Zhang,Xingguang Su
Herein, we developed a novel and sensitive ratiometric fluorescence strategy for pH sensing and urea detection based on MoS 2 quantum dots (MQDs) and 2, 3-diaminophenazine (DAP). The DAP was pH-sensitive and can be used as pH indicator while MQDs was pH-insensitive and acted as a reference fluorescent signal. The fluorescence intensity of DAP at 568 nm exhibited increases gradually while the fluorescence intensity of MQDs at 420 nm kept unchanged as the pH changed from 3.0 to 9.0. So the ratio of the two fluorescence intensities at 568 nm and 420 nm can be used to monitor the change of pH. The pH-sensing system showed a linear and reversible respond to pH in the range of 3.8–6.0 with an interval of 0.2 pH unit. Furthermore, the enzymatic reaction of urea in the presence of urease can lead to the pH increase of the solution, which can be used to detect urea by using the above-mentioned pH-sensing system. There exhibited a good linear relationship between the ratiometric fluorescence intensity of I 568 /I 420 and urea concentrations ranging from 5 to 700 μM. And a low detection limit of 1.8 μM was obtained. The proposed strategy exhibited excellent selectivity and sensitivity. Additionally, the developed ratiometric fluorescence strategy was successfully applied to detect urea in water samples.
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BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Zihong Sun,Zhigang Luo,Cuifen Gan,Shidong Fei,Yingju Liu,Hongtao Lei
A sensitive electrochemical immunosensor for 3-bromobiphenyl (3-BBP) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (PDOP/PB/CMK-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-HRP-DHCNTs-Ab 2 ) as the signal label. PB/CMK-3 was firstly successfully in-situ synthesized with the aid of the CMK-3 reduction, which was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR), X-ray diffraction (XRD) and N 2 adsorption–desorption analysis. By using PDOP/PB/CMK-3 as the substrate, it can effectively enhance the specific surface for antigen loading due to the three-dimensional structure of the nanocomposites, while large amount of PB that fixed inside or outside the pore of CMK-3 successfully improved the electrochemical response and the PDOP film can provide a biocompatible environment to maintain the activity of antigen availability. Under the optimized conditions, the proposed immunosensor shows a good current response to 3-BBP in a linear range from 5 pM to 2 nM with a detection limit of 2.25 pM. In addition, the specificity, reproducibility and stability of the immunosensor were also proved to be acceptable, indicating its potential application in environmental monitoring.
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ELECTROPHORESIS
·
2012
·Vol. 34
·期 4
·510-517
·
IF 3.303
Huihui Yang,Yingzhuang Chen,Yixuan Liu,Lihua Nie,Shouzhuo Yao
A novel (3-sulfopropyl methacrylate potassium)-silica hybrid monolithic column for CEC has been prepared by a simple one-pot approach based on efficient thiol-ene click chemistry. In this process, the polycondensation of hydrolyzed alkoxysilanes and in situ click reaction of vinyl groups on 3-sulfopropyl methacrylate potassium and thiol groups on the precondensed siloxanes simultaneously occurred in a pretreated capillary. Homogeneous monolithic matrix with large through-pores tightly bonded to the inner wall of the capillary was shown by optical microscope and SEM. The minimum plate height of this hybrid monolithic column was determined as 3.9 μm for thiourea. Anilines, alkylbenzenes, and phenols were well separated on this hybrid monolithic column by CEC, which indicated typical reversed-phase and cation-exchange chromatographic retention mechanisms of the column.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Jianwei Dao,Liang Yang,Zhihao Jiang,Zihao Han,Tiqin Wei
Narrow-band red inorganic phosphors excited by near-ultraviolet (n-UV) light have significant application potential in the manufacture of white light-emitting diodes (LEDs) with high R9 index. Novel near-UV-excitable Eu 3+ doped K 3 Ca 2 (SO 4 ) 3 × (X = F, Cl, Br) halosulphate phosphors were synthesized via high-temperature solid-state reaction method. By modifying the non-activating ions in the host matrix, the luminescence properties of Eu 3+ were effectively enhanced across various matrices, thereby optimizing the performance of this series of phosphors. As-prepared phosphors can be activated under 397 nm excitation and emit red light at 619 nm, corresponding to 5 D 0 → 7 F 2 transitions of Eu 3+ . Optimization of the doping concentration revealed that the optimal Eu 3+ concentration for different host matrices is 0.13. Notably, the K 3 Ca 2 (SO 4 ) 3 F host matrix doped with Eu 3+ exhibits stronger emission intensity and better performance. The K 3 Ca 2 (SO 4 ) 3 F:0.13Eu 3+ phosphor exhibited long fluorescence lifetime of 0.42 ms and high color purity of 92.25 %. The emission intensity of K 3 Ca 2 (SO 4 ) 3 F:0.13Eu 3+ at 423 K can retain 89.5 % of its value at room temperature. A white LEDs device was fabricated by mixing and encapsulating K 3 Ca 2 (SO 4 ) 3 F:0.13Eu 3+ phosphor with commercial BaMgAl 10 O 17 :Eu 2+ blue and (Ba, Sr) 2 SiO 4 :Eu 2+ green phosphors onto 397 nm n-UV InGaN chip. The device emits bright white light and exhibits high color rendering index (Ra = 79.9, R9 = 86.5) and a suitable correlated color temperature (CCT = 5915 K) at 50 mA drive current. The results suggest that this work provides new assistance in achieving high color rendering white LEDs excited by n-UV light.
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ACS Applied Polymer Materials
·
2024
·
IF 4.4
Haifeng Yu,Yanting Du,Jiayu Li,Songhao Zhao,Cheng Wang,Shuhong Wang,Haiyang Chang
Donor–acceptor (D–A) conjugated polymers have exhibited their utility in the fabrication of memory devices with high information storage capacity. The device performance can be effectively regulated through the appropriate pairing of the acceptor and donor moieties. Six different D–A conjugated polymers, featuring varied structures, were synthesized by using the Suzuki coupling reaction. Donor molecules such as fluorene, carbazole, and 4′-methoxytriphenylamine, as well as the acceptor molecule dipyrido[3,2-a:2′,3′-c]phenazine (dppz), were employed in this process. Interestingly, it was observed that simply increasing the ratio of donor groups did not yield improvements in device performance. On the other hand, the memory devices fabricated using these polymers as the active layers exhibited favorable ternary resistance memory behavior. Specifically, the first and second threshold voltages were impressively low, at −0.40 and −0.75 V, respectively. The OFF: ON1: ON2 current ratio reached an outstanding ratio of 1:102.90:105.47. Moreover, these devices demonstrated excellent stability with a retention time of 104 s and an endurance of 103 cycles. In conclusion, this study successfully synthesized D–A conjugated polymer with dppz as an acceptor and proved the great potential of constructing ternary memory devices using the dppz structure.
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NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
·
2021
·
IF 2.9
Yonglu Li,Yaxuan Wang,Xin Yu,Ting Yu,Xiaodong Zheng,Qiang Chu
Lung cancer with high mortality is regarded as a challenging global problem with unsatisfied curative effects. Clinically, the chemotherapy drugs are often faced with side-effects and tumor resistance. Radix Tetrastigma (RT) is a traditional Chinese herb and now regarded as a kind of functional food. In this study, A549-bearing nude mice control was adopted to evaluate the anti-tumor capacity of RT. Results demonstrated that RT showed excellent anti-tumor ability with no side-effect on mice compared to chemotherapy drug (5-Fu).Further studies proved that RT down-regulated the proliferation-related proteins (PCNA, Ki67) and vascular endothelial growth factor (VEGF). Additionally, RT up-regulated the ratio of Bax/Bcl-2, which caused the over-expression of Caspase-9, leading to the activation of downstream protein caspase-3, eventually resulting in apoptosis of A549 in solid tumor. These results together suggest that RT inhibits the non-small cell lung cancer (NSCLC) via Bax/Bcl2/Caspase-9/Caspase-3 pathway. Furthermore, the anti-A549 abilities of the main flavonoid components from RT were compared, and kaempferol-3-O-rutinoside would play a role in RT’s outstanding anti-NSCLC ability.
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Yang Bin,Sun Yiwen,Fu Shouying,Xia Miaomiao,Su Yuan,Liu Chuan,Zhang Chunzhi,Zhang Dawei
Ribulose 5-phosphate (Ru5P) and guanosine 5′-triphosphate (GTP) are two key precursors of riboflavin, whereby Ru5P is also a precursor of GTP. Ribulose 5-phosphate 3-epimerase (Rpe) catalyzes the conversion of ribulose 5-phosphate into xylulose 5-phosphate. Inactivation of Rpe can reduce the consumption of Ru5P, enhancing the carbon flux toward riboflavin biosynthesis. Here we investigated the effect of mutation of rpe and other related genes on riboflavin production, physiological and metabolic phenotypes in Bacillus subtilis LY (BSLY). Introducing single nucleotide deletion (generated BSR) or nonsense mutation (generated BSRN) on the genomic copy of rpe, resulting in more than fivefold increase of riboflavin production over the parental strain. BSR process 62% Rpe activity, while BSRN lost the entire Rpe activity and had a growth defect compared with the parent strain. BSR and BSRN exhibited increases of the inosine and guanine titers, in addition, BSRN exhibited an increase of inosine 5′-monophosphate titer in fermentation. The transcription levels of most oxidative pentose phosphate pathway and purine synthesis genes were unchanged in BSR, except for the levels of zwf and ndk, which were higher than in BSLY. The production of riboflavin was increased to 479.90 ± 33.21 mg/L when ribA was overexpressed in BSR. The overexpression of zwf, gntZ, prs, and purF also enhanced the riboflavin production. Finally, overexpression of the rib operon by the pMX45 plasmid and mutant gnd by pHP03 plasmid in BSR led to a 3.05-fold increase of the riboflavin production (977.29 ± 63.44 mg/L), showing the potential for further engineering of this strain.
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ANALYST
·
2013
·Vol. 138
·期 6
·1851-1857
·
IF 3.969
Huanshun Yin,Zhenning Xu,Yunlei Zhou,Mo Wang,Shiyun Ai
A label-free electrochemical immunosensor for ultra-sensitive detection of indole-3-acetic acid (IAA), a very important phytohormone, has been developed in this work. The detection strategy was mainly based on 4-aminophenylboronic acid, magnetic nanoparticles functionalized with horseradish peroxidase-conjugated goat anti-rat immunoglobulin G (HRP-IgG-Fe3O4) and rat monoclonal antibody against IAA-modified gold nanoparticles (anti-IAA-AuNPs). HRP-IgG-AuNPs was covalently assembled on the electrode surface through the specific chemical reaction between boronic acid and the vicinal diol in HRP-IgG. Then, anti-IAA-AuNPs was further assembled on the electrode via the interaction between IgG and antibody. Through the dual amplification of HRP-IgG-Fe3O4 and anti-IAA-AuNPs, the trapping capacity of the immunosensor for IAA was significantly enhanced based on the promotion of the immunoreaction between antibody and antigen, which resulted in a large decrease of the electrochemical response of the redox probe, Fe(CN)63−, and an increase in sensitivity. The developed electrochemical immunosensor exhibited a wide linear range from 0.02 to 500 ng mL−1 with a low detection limit of 0.018 ng mL−1 (S/N = 3). Moreover, the proposed immunosensor showed acceptable selectivity, reproducibility, accuracy and stability. The IAA extracted from various seeds was successfully detected using the developed immunosensor. This assay method might provide an alternative strategy for the detection of various phytohormones.
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JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Zhiyuan Cheng,Yijie Wang,Yao Zhang,Chan Zhang,Mengru Wang,Wei Wang,Jiacheng He,Yang Wang,Hankun Zhang,Qiansen Zhang,Chunyong Ding,Deyan Wu,Linlin Yang,Mingyao Liu,Weiqiang Lu
Nowadays, small-molecule drugs have become an indispensable part of tumor immunotherapy. Accumulating evidence has indicated that specifically blocking PGE2/EP4 signaling to induce robust antitumor immune response represents an attractive immunotherapy strategy. Herein, a 2H-indazole-3-carboxamide containing compound 1 was identified as a EP4 antagonist hit by screening our in-house small-molecule library. Systematic structure–activity relationship exploration leads to the discovery of compound 14, which displayed single-nanomolar EP4 antagonistic activity in a panel of cell functional assays, high subtype selectivity, and favorable drug-like profiles. Moreover, compound 14 profoundly inhibited the up-regulation of multiple immunosuppression-related genes in macrophages. Oral administration of compound 14, either as monotherapy or in combination with an anti-PD-1 antibody, significantly impaired tumor growth via enhancing cytotoxic CD8+ T cell-mediated antitumor immunity in a syngeneic colon cancer model. Thus, these results demonstrate the potential of compound 14 as a candidate for developing novel EP4 antagonists for tumor immunotherapy.
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