Scientific Reports
·
2016
·Vol. 6
·期 1
·1-11
·
IF 5.228
Jiao Jiajia,Sun Lili,Guo Zaiyu,Hou Sen,Holyst Robert,Lu Yun,Feng Xizeng
Polydimethylsiloxane (PDMS) is widely used as a cell culture platform to produce micro- and nano-technology based microdevices. However, the native PDMS surface is not suitable for cell adhesion and is always subject to bacterial pollution and cancer cell invasion. Coating the PDMS surface with antibacterial or anticancer materials often causes considerable harm to the non-cancer mammalian cells on it. We have developed a method to fabricate a biocompatible PDMS surface which not only promotes non-cancer mammalian cell growth but also has antibacterial and anticancer activities, by coating the PDMS surface with a Chinese herb extract, paeonol. Coating changes the wettability and the elemental composition of the PDMS surface. Molecular dynamic simulation indicates that the absorption of paeonol onto the PDMS surface is an energy favourable process. The paeonol-coated PDMS surface exhibits good antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover considerable antibacterial activity is maintained after the coated surface is rinsed or incubated in water. The coated PDMS surface inhibits bacterial growth on the contact surface and promotes non-cancer mammalian cell growth with low cell toxicity; meanwhile the growth of cancer cells is significantly inhibited. Our study will potentially guide PDMS surface modification approaches to produce biomedical devices.
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PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Jun Ni,Rujue Ruan,Lijun Wang,Zhifang Jiang,Xiajun Gu,Lisha Chen,Maojun Xu
Anthocyanins, a class of flavonoids, are synthesized in a branch of the flavonoid pathway. Dihydroflavonol-4-reductase (DFR) catalyzes the first reaction leading to anthocyanins, and it is considered a key regulatory enzyme of anthocyanin biosynthesis in plants. In this research, we isolated three new DFR genes by searching the whole-genomic sequence data of Ginkgo biloba . The genes have similar structures and are clustered in the same scaffold. Sequence analyses of GbDFR proteins revealed that these GbDFRs have a closer relationship to Arabidopsis thaliana DFRs than previously identified GbDFRs. Expression analyses revealed that GbDFR4 and GbDFR6 were preferentially expressed in leaves and fruit, respectively. Young and mature leaves shared similar gene expression patterns, while fruit harvested in May had significantly higher expression levels of GbDFR s than fruit harvested in June. Transgenic tobacco plants ectopically overexpressing GbDFR genes showed higher DFR enzymatic activity levels, higher anthocyanin contents and darker flowers than the control. Ginkgo seedlings treated with NaCl showed increased total flavonoid and anthocyanin contents, and the expression levels of GbDFR genes were also induced. In contrast, UV-B irradiation increased the total flavonoid content but decreased both the anthocyanin content and GbDFR expression levels. A salicylic acid treatment induced and inhibited the total flavonoid content with and without light, respectively. In contrast, both the GbDFR expression levels and the anthocyanin content were inhibited by the salicylic acid treatment regardless of the light conditions. This research validated the functions of GbDFRs and expands our understanding of the environmental stress-response mechanisms of ginkgo trees.
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高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3369
·
IF 3.098
Li-Min Zhao,Zhen Guo,Yi-Jie Xue,Jun Zhe Min,Wen-Jing Zhu,Xiang-Yu Li,Hu-Ri Piao,Cheng Hua Jin
The transforming growth factor-β (TGF-β), in which overexpression has been associated with various diseases, has become an attractive molecular target for the treatment of cancers. Thirty-two quinoxaline-derivatives of 3-substituted-4-(quinoxalin-6-yl) pyrazoles14a–d,15a–d,16a–d,17a–d,18a–d,19a–d,25a,25b,25d,26a,26b,26d,27b, and27dwere synthesized and evaluated for their activin TGF-β type I receptor kinase and p38α mitogen activated protein (MAP) kinase inhibitory activity in enzymatic assays. Among these compounds, the most active compound19binhibited TGF-β type I receptor kinase phosphorylation with an IC50value of 0.28 µM, with 98% inhibition at 10 µM. Compound19balso had good selectivity index of >35 against p38α MAP kinase, with 9.0-fold more selective than clinical candidate, compound3(LY-2157299). A molecular docking study was performed to identify the mechanism of action of the synthesized compounds and their good binding interactions were observed. ADMET prediction of good active compounds showed that these ones possess good pharmacokinetics and drug-likeness behavior.Keywords:TGF-β;ALK5 inhibitor;kinase assay;selectivity;docking
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高影响因子
RSC Advances
·
2021
·Vol. 11
·期 6
·3470-3475
·
IF 3.361
Ye Meng,Weiwei Zhao,Jun Zheng,Daofa Jiang,Jie Gao,Yanmei Jin,Peihua Ma
Since the first reportal on decamethylcucurbit[5]uril (Me10Q[5]) in 1992, substituted cucurbit[n]urils have attracted considerable research interest. In this study, the host–guest modes between the tetramethyl cucurbit[6]uril (TMeQ[6]) as a host and 4-chloroaniline and 4,4′-diaminostilbene (G1 and G2) as guests were investigated by single-crystal X-ray diffraction, NMR, ITC, UV-Vis spectrum, and MALDI-TOF mass spectrometry analyses. The experimental results showed that TMeQ[6] formed a 1 : 1 inclusion compound with G1, and the carbonyl portal of TMeQ[6] formed a 1 : 1 self-assembly with G2. Further, multi-dimensional supramolecular frameworks were formed driven by weak interaction forces in the system (hydrogen bonding, C–H⋯π interactions, ion–dipole interactions, and dipole–dipole interactions).
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高影响因子
RSC Advances
·
2018
·Vol. 8
·期 8
·4119-4124
·
IF 2.936
Pengpeng Zhang,Qing Yu,Xue Min,Linbao Guo,Jiangjian Shi,Xiangyun Zhao,Dongmei Li,Yanhong Luo,Huijue Wu,Qingbo Meng,Sixin Wu
The annealing temperature of solution-processed Cu2ZnSn(S,Se)4 (CZTSSe) precursor films has been carefully optimized for favorable selenization. At higher temperature, more solvent will be removed, and the crystallinity of the precursor films can be improved. It is found that, although the crystallinity of selenized film is continuously enhanced by increasing the temperature, a dense CZTSSe film with round and large grains can only be achieved at medium high temperature ranging from 350 to 400 °C. Further investigation reveals that, in this regime, the carrier and defect densities are obviously reduced, leading to average photoelectric conversion efficiency (PCE) improved from 5.1% to 9.4%. An optimal annealing temperature of 380 °C is obtained and up to 10.04% of photoelectric conversion efficiency has been achieved.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-11
·
IF 5.833
He Yanlei,Li Ning,Liu Xiangwei,Chen Wei,Zhu Xixi,Liu Qingyun
Urchin-like peroxidase mimics 5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized CuCo 2 O 4 nanospheres (Por-CuCo 2 O 4 ) has been fabricated as an excellent visual biosensor. X-ray diffractometry (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) have been employed to characterize the composition, morphologies, and elemental analysis of the as-synthesized Por-CuCo 2 O 4 . The catalytic activity of Por-CuCo 2 O 4 was evaluated by the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with the aid of H 2 O 2 , which exhibited a visual blue change with an absorption maximum at 652 nm for only 10 s. The peroxidase-like behaviors of Por-CuCo 2 O 4 conformed to the Michaelis-Menten equation. Electrochemistry, radical scavenger, and fluorescence probe experiments verified that electron transfer, •O 2 − radicals, and holes (h + ) are the important factors during the catalytic oxidation of TMB. Based on the inhibition of dopamine (DA) on TMB oxidation, the Por-CuCo 2 O 4 -based colorimetric biosensor has been successfully constructed for sensitive determination of DA witha detection limit (LOD) of 0.94 μΜ. In addition, colorimetry was validated to detect DA in serum samples with high sensitivity and good selectivity. Graphical abstract
5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized urchin-like CuCo 2 O 4 (Por-CuCo 2 O 4 ) with excellent peroxidase activity, ascribed to the synergistic effect between •O 2 − radicals and holes (h + ). A fast colorimetric sensor on the basis of Por-CuCo 2 O 4 has been constructed to quantitatively determine dopamine concentration in human serums.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2010
·
IF 3.083
Jiadong Huang,Xiuming Zhang,Su Liu,Qing Lin,Xiaorui He,Xianrong Xing,Wenjing Lian,Di Tang
4-Nonylphenol (4-NP) was reported to affect the health of wildlife and humans through altering endocrine function. A novel electrochemical sensor for sensitive and fast determination of 4-NP was developed. Titanium oxide (TiO 2 ) nanoparticles and gold nanoparticles (AuNPs) were introduced for the enhancement of electron conduction and sensitivity. 4-NP-imprinted functionalized AuNPs composites with specific binding sites for 4-NP was modified on electrode. The resulting electrodes were characterized by cyclic voltammetry (CV). Rebinding experiments were carried out to determine the specific binding capacity and selective recognition. The linear range was over the range from 4.80 × 10 −4 to 9.50 × 10 −7 mol L −1 , with the detection limit of 3.20 × 10 −7 mol L −1 ( S / N = 3). The sensor was successfully employed to detect 4-NP in real samples.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiang Pu,Jiahua Zhang,Jinwei He,Zhihui Ai,Xiaoxue He,Xiaojun Zhou,Shiyuan Tong,Xinyue Dai,Qiqi Wu,Jiayu Hu,Jingshu He,Hanguang Wang,Wei Wang,Jinqiu Liao,Li Zhang
The biosynthetic route for flavonol in Camptotheca acuminata has been recently elucidated from a chemical point of view. However, the genes involved in flavonol methylation remain unclear. It is a critical step for fully uncovering the flavonol metabolism in this ancient plant. In this study, the multi-omics resource of this plant was utilized to perform flavonol O -methyltransferase-oriented mining and screening. Two genes, CaFOMT1 and CaFOMT2 are identified, and their recombinant CaFOMT proteins are purified to homogeneity. CaFOMT1 exhibits strict substrate and catalytic position specificity for quercetin, and selectively methylates only the 4′-OH group. CaFOMT2 possesses sequential O -methyltransferase activity for the 4′-OH and 7-OH of quercetin. These CaFOMT genes are enriched in the leaf and root tissues. The catalytic dyad and critical substrate-binding sites of the CaFOMTs are determined by molecular docking and further verified through site-mutation experiments. PHE181 and MET185 are designated as the critical sites for flavonol substrate selectivity. Genomic environment analysis indicates that CaFOMT s evolved independently and that their ancestral genes are different from that of the known Ca10OMT . This study provides molecular insights into the substrate-binding pockets of two new CaFOMTs responsible for flavonol metabolism in C. acuminata .
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高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2024
·
IF 2
Guofang Jiang,Can Zhang,Xiaohong Xing,Jinbo Ouyang,Hong Yang,Zongbo Xie,Zhanggao Le
A novel and highly efficient method for the synthesis of 1-(4-hydroxybutyl)-2-benzoyl indoles from 2-pyrrolidine benzaldehyde and α -bromoacetophenone in a choline chloride (ChCl)/ZnCl 2 eutectic mixture was developed. This method, which does not require any catalyst, additive, or alkali, is useful for realizing tandem cyclization reactions. Several indoles were produced after cyclization and ring opening processes. Not all the compounds obtained are reported in the literature. This synthetic strategy proves that deep eutectic solvents can replace catalysts and organic solvents. Consequently, this is an environmentally friendly strategy that has significant potential in the development of green and sustainable chemistry.
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高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 13
·135503
·
IF 3.953
Shuyang Hu,Qiuyan Shuai,Yulong Lin,Yan Fu,Meng Li
L-3,4-dihydroxy-phenylalanine (L-dopa) is the most widely used drug in Parkinson's disease treatment. However, development of cost-effective and high-throughput sensors to accurate enantioselective discrimination of L-dopa and D-dopa remains challenging to date. Herein, on the basis of the peroxidase-mimic activity of chiral FexCuySe nanoparticles, we demonstrated a novel colorimetric sensor for determination of chiral dopa. The surface chiral ligand, L/D-histidine (L/D-His), endowed the nanozymes with enantioselectivity in catalyzing the oxidation of dopa enantiomers. According to the values of kcat/Km, the efficiency of L-His modified nanoparticles (L-FexCuySe NPs) towards L-dopa was 1.56 times higher than that of D-dopa. While, D-His can facilely reverse the preference of the nanozyme to D-dopa. On the basis of high catalytic activity and enantioselectivity of L-FexCuySe NPs in oxidation of L-dopa, the L-FexCuySe NPs-based system can be utilized for detection of L-dopa. The linear ranges for L-dopa determination were 5 μM–0.125 mM and 0.125 mM–1 mM with a detection limit of 1.02 μM. Critically, the developed sensor has been successfully applied in the quality control of clinical used L-dopa tablets. Our work sheds light on developing simple and sensitive chiral nanomaterials-based sensors for drug analysis.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Haiyang Zhang,Hongyan Zhang,Zhenhong Jia,Chu Chen,Chen Yang,Qiaoya Dou,Xudong Li,Xiujuan Ma,Pengfei Ding
Organic-inorganic hybrid materials hold great application prospects in electronic devices such as humidity sensors due to their unique interfacial effects and synergistic interactions. However, relatively few studies have been conducted on the sensitization mechanism of organic–inorganic hybrid materials. Herein, we report a high performance humidity sensor based on an organic-inorganic hybrid material of poly(sodium 4-styrenesulfonate)-modified amino-functionalized SnO 2 (PSS-SnO 2 ). By employing the PSS surface modification strategy, the content of sulfonic groups (-SO 3 H) and oxygen vacancies on the surface of SnO 2 was significantly increased, which effectively enhanced its humidity sensing performance. The results reveal that the PSS-SnO 2 sensor has a higher response (18421.6), lower humidity hysteresis (1.8% RH) and faster response/recovery (2.8/5.7 s) compared to the SnO 2 sensor. Experimental analysis and density functional theory (DFT) results show that the presence of oxygen vacancies and -SO 3 H effectively promotes the dissociation of water molecules, resulting in more H + conduction on the surface of SnO 2 , which improves the sensitivity and response time of the sensor. Furthermore, the PSS-SnO 2 humidity sensor realizes visual monitoring of plant growth environments, which shows the potential in the field of smart agriculture.
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Hua Yu,Bingxue Yao,Degang Deng,Leifeng Chen,Hongting Zhao,Yinqun Li,Youjie Hua,Shiqing Xu
A novel tunable red to yellow emitting phosphor, (Ca, Ba) 4 (PO 4 ) 2 O:Eu 2+ is reported that displays a broad emission from 500 to 800 nm, and its emitting color can be adjusted from red to yellow by changing Ba 2+ doping concentration. X-ray powder diffraction (XRD) analysis confirmed the phase formation. Excitation and emission spectra, and concentration dependence of emission intensity of the phosphor were investigated. The results showed that the emission peak wavelength take blue-shifted from 640 to 545 nm and the color hue can be tuned from red to yellow with increasing Ba 2+ concentration. When single-phase (Ca 3.85 Ba 0.10 )(PO 4 ) 2 O:0.05Eu 2+ phosphor is pumped by a blue InGaN light-emitting diode, we obtain white light with color rending index ( R a ) between 84.0 and 90.6 and color temperatures between 4000 and 5500 K, which suggests that this material is competitive as a color conversion material for solid state lighting.
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高影响因子
危险品化学品经营许可证（带存储）