INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.5
Wen Zhu,Xinya Mo,Hong Chen,Yuyang Zhang,Jianan Sun,Jiao Li,Sai Wang,Xiangzhao Mao
Norfloxacin (NOR) residue in animal-derived food poses a significant concern due to its role in fostering bacterial resistance and potential human health risks, necessitating rapid and sensitive detection methods. In this study, a colorimetric DNA chimera biosensor was developed by integrating a bivalent split aptamer pair with G-quadruplex DNAzyme for highly sensitive NOR detection. Molecular docking studies were performed to identify the key binding regions, facilitating aptamer truncation and splitting. The bivalent strategy markedly enhanced binding affinity. The presence of NOR guides the self-assembly of the functional DNA chimera, activating the G-quadruplex/hemin DNAzyme to catalyze ABTS oxidation and generate colorimetric signals. The biosensor demonstrated a linear range of 3.75–960 nM and a limit of detection of 2.97 nM, with excellent specificity. When applied to real seafood samples, it showed good accuracy and repeatability with recovery rates of 87.87–112.59%, offering a reliable platform for rapid detection of fluoroquinolone residues in seafood.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2026
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IF 5.1
Liang Gong,Lin Zhu,Wencheng Xu,Xiuzhi Shan,Miduo Tan,Yubin Gu,Juan Zhou,Sihua Liu
Influenza virus infections, particularly those caused by the Avian influenza virus, result in millions of deaths and substantial economic losses annually. Early diagnosis of the pathogen during the initial stages of an outbreak is pivotal for effective outbreak control. This study presents the development of an Exo III-mediated high sensitivity dual signal amplification (HSDSA) platform for detecting the H5N1 gene sequence. The detection mechanism is initiated by the hybridization of the target H5N1 DNA with hairpin DNA (Hp DNA) to form double-stranded structure. Hp DNA in this structure could be recognized and partial hydrolyzed by Exo III, releasing target DNA and free DNA. On one side, the target DNA enabled hybridize with other Hp DNA for circulate freely, achieving the first round of amplification. On the other hand, free DNA can hybridize with the DNA structure labeled on the gold nanoparticles, transforming the originally 3-terminal protruding double-stranded structure into a 3-terminal concave Exo III substrate structure. After triggering the Exo III recognition, the long chains (SH DNA) within dsDNA are hydrolyzed, and free DNA is released again to enter the next round of triggering for the second round of amplification. Meanwhile, another DNA labeled with carboxyfluorescein (FAM DNA) released from the hydrolyzed SH DNA, causing the fluorophore to detach from the AuNPs, the fluorescence signal is restored. This strategy significantly enhances detection sensitivity through double cyclic signal amplification mediated by Exo III, with a linear range from 0.08 nM to 15 nM and a detection limit of 54 pM for H5N1 DNA. The fluorescence sensor demonstrates robust applicability in detecting H5N1 in human serum samples, with recovery rates ranging from 98.75% to 105.83%. These results underscore the potential of the HSDSA platform for low-abundance molecular detection, disease diagnosis, and biomedicine applications.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2015
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IF 2.96
Meiju Niu,Min Hong,Guoliang Chang,Xiao Li,Zhen Li
Five transition metal complexes NiL 1 2 ( 1 ), CuL 1 2 ( 2 ), ZnL 1 2 ( 3 ), [MnL 1 2 (N 3 )] n · n CH 2 Cl 2 ( 4 ), CuL 2 2 ( 5 ) {HL 1 = 3-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-naphthalen-2-ol, HL 2 = 2-{[2-(2-hydroxy-ethoxy)-ethylimino]-methyl}-phenol} have been synthesized and fully characterized. In all of the complexes, the ligands coordinated to the metal ion in a negative fashion via O and N donor atoms. The X-ray structures of nickel complex 1 and copper complexes 2 and 5 are four-coordinated monomers and show slightly distorted square-planar geometry in the vicinity of the central metal atom. Zinc complex 3 exhibits a four-coordinated tetrahedral structure. Differently, manganese complex 4 reveals a six-coordinated octahedral structure, one-dimensional chain is linked by azide in the end-to-end mode. In vitro cytotoxicity of these complexes to various tumor cell lines was assayed by the MTT method. The results showed that most of these metal-Schiff base complexes exhibited enhanced cytotoxicity than Schiff base ligands, which clearly implied a positive synergistic effect. Moreover, these complexes appeared to be selectively active against certain cell lines. The interactions of these metal complexes with CT-DNA were investigated by UV–vis, fluorescence and CD spectroscopy, the results indicated that these complexes are metallointercalators and can interact with CT-DNA. The study of interaction between complexes and BSA indicated that all of the complexes could quench the intrinsic fluorescence of BSA in a static quenching process.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2026
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IF 4.7
Li Zhou,Xianwei Jiao,Yuting Zhang,Changling Yan
L-selectin, a leukocyte adhesion receptor, is essential in the early step of the adhesion cascade, facilitating the migration of leukocytes into surrounding tissues during inflammation and immune surveillance. Clinical studies have identified L-selectin as a prospective treatment option for many both acute and ongoing inflammatory conditions. Clarifying the cellular signaling pathways that function in the immediate vicinity of L-selectin may yield unique treatments tailored to certain cell types or illnesses. The study describes the development of a precise and sensitive fluorescence biosensor designed for the detection of L-selectin using a split aptamer specific to L-selectin. This biosensor utilizes nicking enzyme-assisted DNA walking together CRISPR-Cas12a for dual-signal multiplication. The calibration curve for L-selectin demonstrates a detection limit of 0.45 ng/mL, with an extensive linear range spanning from 10 to 1280 ng/mL. The fluorometric sensors effectively identified L-selectin in human blood samples, producing excellent outcomes. The fluorometric sensors effectively identified L-selectin in human blood samples, producing excellent outcomes. The original appearance not only validates the concept but also possesses substantial possibilities for identifying diverse targets.
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INFRARED PHYSICS & TECHNOLOGY
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2026
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IF 3.8
Lu Chen,Xili Jing,Tianli Huo,Dongfang Li,Mingshi Song,Shuguang Li
Currently, the high-precision quantification of disease-related complementary DNA (cDNA) remains difficult, owing to the complex procedures of existing nucleic acid detection methods and their susceptibility to temperature fluctuations. Therefore, a dual-film-modulated fiber-optic surface plasmon resonance (SPR) biosensor with self-temperature compensation was proposed in this work, enabling high-precision detection of cDNA concentration through real-time temperature readout for in-situ temperature correction to improve the controllability of the measurement process. A low-loss no-core fiber (NCF) was used as the sensing platform, with an Ag film as the SPR excitation layer. A CeF 3 film was introduced into the DNA channel to enhance the interfacial response, while a TiO 2 layer was introduced into the temperature channel to regulate the resonance band and enable thermal readout. Simulations verified the sensitization effect of CeF 3 and the resonance wavelength modulation effect of TiO 2 . Further single-channel calibration and cascaded tests showed that the two channels operated independently with negligible crosstalk. The experimental results demonstrated that, over a cDNA concentration range of 0-90 nM, the DNA probe exhibited a maximum sensitivity of 0.412 nm/nM, an average sensitivity of 0.294 nm/nM, and a limit of detection (LOD) of 1.62 nM. Compared with conventional single-layer Au/Ag fiber-optic SPR nucleic acid sensors, the proposed sensor, with the synergistic integration of a CeF 3 sensitization layer and temperature compensation, achieves more accurate DNA quantification and a more competitive LOD. Its low cost and streamlined fabrication process further enhance its value for large-scale deployment, underscoring its strong potential in biomedical applications such as disease monitoring.
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ADVANCED FUNCTIONAL MATERIALS
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2026
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IF 19.9
Rui Xu,Jingcheng Huang,Junhao Zhao,Shouyi Dou,Zhiheng Zhu,Zheng Shen,Heng Zhang,Lingjun Geng,Jiashuai Sun,Dianbin Su,Jicheng Zhao,Fangling Du,Xia Sun,Yemin Guo,Jianfeng Ping
Organophosphorus pesticides (OPs) are widely applied for crop pest control, but human health and the environment are seriously threatened by their excessive use. Therefore, rapid detection of pesticide residues is of great importance. In this work, a flexible aptasensor was developed by integrating an indium oxide electrolyte-gated field-effect transistor (EG-FET) with a surface-adhered DNA hydrogel obtained via rolling circle amplification (RCA) for in situ detection of OPs residues. The target pesticides were specifically recognized by the responsive DNA hydrogel, which underwent structural collapse and consequently modulated carrier depletion in the n-type FET channel. Furthermore, a wireless detection device based on a dual-channel potentiostat and a smartphone-based human-machine interface was established, enabling the detection of four OPs, including omethoate (OMT), phorate (PHO), isocarbophos (ICP), and chlorpyrifos (CPF). The EG-FET aptasensor exhibited a good linear relationship between signal response and pesticide concentration in the range of 1 pg/mL–10 ng/mL, with the limit of detection of 0.244, 0.273, 0.132, and 0.301 pg/mL, respectively. The developed device was characterized by compactness and low-cost fabrication. This work proposes a new approach to detecting small-molecule contaminants, opening avenues for transforming agricultural, food safety, and environmental monitoring from passive post-event detection to active process control.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Gengchen Hu,Shizhao Liu,Yiming Zhang,Yao Jiang,Dongmei Xi
The widespread use of aminoglycoside antibiotics has raised serious concerns regarding food safety and environmental health, necessitating advanced methods for their sensitive and multiplexed detection. However, conventional techniques struggle to directly quantify these small molecules with sufficient sensitivity and specificity. Here, we present an ultrasensitive and simultaneous sensing platform for kanamycin (KAN) and tobramycin (TOB) that synergistically merges a target-triggered DNA walker with a biological α-hemolysin (α-HL) nanopore. In this design, aptamer recognition of the antibiotic activates a DNAzyme-based walker immobilized on magnetic beads, which then processively cleaves a track strand to release hundreds of DNA reporter probes per binding event. This dual-amplification strategy of catalytic chemical amplification coupled with single-molecule physical readout enables exceptional sensitivity, achieving detection limits as low as 3.88 fM for KAN and 4.62 fM for TOB. The sensing platform exhibits excellent selectivity and successfully accomplishes the simultaneous quantification of both antibiotics in a mixture. When applied to spiked environmental water samples, the method demonstrates high accuracy and reliability, yielding satisfactory recovery rates. This work establishes a generalizable DNA walker–nanopore paradigm, offering a versatile blueprint for the development of highly sensitive, multiplexed, and rapid detection systems for a wide range of small-molecule contaminants.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Hui Cao,Run Gao,Lin Chen,Zixia Huang,Xiuxiu Wu,Jinsong Yu,Fei Xu
Hg(II) is a widespread environmental contaminant with high toxicity and severe adverse effects on human health. Although aptamers are commonly used as recognition elements for Hg(II), their binding performance is highly susceptible to pH. In this study, we designed a dual-functional Hg(II)-specific aptamer and developed a biosensor based on Tb(III)-DNA self-assembled coordination polymers (Tb-DNA CPs) for the rapid detection of Hg(II). In the designed aptamer, the G-rich region functions as a luminescent antenna ligand to sensitize Tb(III) emission, while the T-rich region serves as the Hg(II) recognition unit. The resulting Tb-DNA CPs retained 90.2% of their original fluorescence intensity after 30 days in aqueous solution and demonstrated high stability over a broad pH range (pH 4–11). This improved stability is attributed to the role of Tb(III) as a structural crosslinker that promotes the assembly of coordination polymers. The sensor exhibited a limit of detection (LOD) of 3.89 nM for Hg(II), with a linear response range of 5 to 250 μg·L −1 . In vegetable samples, Hg(II) was rapidly quantified with recoveries of 96.49% to 113.40% and RSDs of 1.98%–4.83%. Mechanistic analysis indicated that the sensing response of the Tb-DNA CPs was mainly driven by photoinduced electron transfer (PET) and Hg(II)-induced conformational changes. This work overcomes the pH-dependent limitation of conventional aptamer-based sensors and provides a practical platform for on-site Hg(II) monitoring. The Tb-DNA CP strategy also offers a versatile foundation for the development of stable lanthanide-based biosensors.
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Analytical Methods
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2026
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IF 3
Tao Fang,Min Shi,Uchkun Ishimov,Awais Ihsan,Ruiting Tian,Jun Li,Xianju Huang,Han Cheng
This study presents the development of a highly sensitive and selective electrochemical sensor based on ruthenium-gold nanocluster-functionalized carbon fiber microelectrodes (AuNPs/RuNPs/CFME) for the ultrasensitive detection of acetaminophen (ACOP) and its genotoxic impurities, p-aminophenol (PAP) and p-nitrophenol (PNP). Leveraging the synergistic electrocatalytic effects of bimetallic nanoparticles, the modified electrode demonstrated exceptional performance, achieving wide linear ranges (0.04–100 µmol L−1 for ACOP, 1–10 µmol L−1 for PAP and PNP) and remarkably low detection limits (6.192 nmol L−1 for ACOP, 0.19 µmol L−1 for PAP, and 0.015 µmol L−1 for PNP). The sensor exhibited outstanding stability, anti-interference capability, and successful application in human serum samples with recoveries of 95.9–100.5%. Furthermore, the study explored the genotoxic interaction between ACOP and double-stranded DNA (dsDNA) using electroanalytical, UV spectrophotometric, and molecular docking techniques. Results revealed that ACOP intercalates into dsDNA via hydrogen bonding and π–π stacking, providing mechanistic insights into its hepatotoxicity at high concentrations. The proposed sensor not only offers a robust platform for multiplexed drug monitoring but also advances understanding of ACOP-induced DNA damage, highlighting its potential in pharmacokinetic studies and toxicity assessment. This work underscores the significance of nanomaterial-enhanced electrochemical sensors for pharmaceutical quality control and toxicity assessment.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Ming Wang,Xingliang Cheng,Yuling Hu,Gongke Li
Conventional meat products are susceptible to bacterial toxin contamination, posing serious risks to food safety. This study presents a portable surface-enhanced Raman scattering (SERS) platform integrated with stimuli-responsive DNA hydrogels and trimetallic nanozymes for the detection of botulinum neurotoxin type A (BoNT/A). The system employs Cu/Au/Pt trimetallic nanozymes immobilized on a steel mesh alongside DNA anchor strands that initiate hydrogel film formation. Through sequential assembly of BoNT/A aptamers and crosslinked DNA strands, a stimuli-responsive hydrogel encapsulating the nanozymes is constructed. In the presence of BoNT/A, the aptamer triggers hydrogel cleavage facilitated by exonuclease I, releasing the nanozymes to catalyze the oxidation of TMB. This reaction generates a visible colorimetric change for rapid visual screening, while the concurrently enhanced SERS signals provide the basis for highly sensitive and quantitative detection. The platform achieves an exceptionally low detection limit of 1 ng/L for BoNT/A within 45 minutes, with a linear SERS response between 5.0 and 400.0 ng/L. Successful application in real meat samples confirms its practicality as a robust, on-site tool for food safety monitoring.
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Cell Death & Disease
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2026
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IF 12.2
Qiu Quanpeng,Ding Yi,Han Jing,Guo Xiaolong,Zhang Jiaqi,Chen Yue,Xue Xinzhu,Hu Xiake,Yu Tianyu,Liu Gaixia,Zhang Haowei,Li Qixin,Li Xiang,She Junjun,Chen Yinnan
The malignant transition of colorectal cancer (CRC) is caused by the combined action of epigenetic dysregulation and genomic instability. It is well known that EZH2-directed H3K27me3 modification is one of the main contributors to tumorigenesis; however, how it affects the DNA mismatch repair (MMR) system and the genesis of extrachromosomal circular DNA (eccDNA) remains unknown. Here, we demonstrate that EZH2 epigenetically represses critical MMR genes, which results in genomic instability and abnormal eccDNA accumulation, ultimately accelerating CRC progression. On the molecular level, the H3K27me3 mark catalyzed by EZH2 is the main cause of the downregulation of MMR genes, which affects the stability of the genome and leads to an increase in eccDNA that makes the tumor more aggressive. Our study reveals an unprecedented role of EZH2 in controlling chromosomal instability and eccDNA production through the MMR pathway and offers a conceptual framework for the development of CRC-targeted epigenetic therapies.
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PubMed
高影响因子
TALANTA
·
2026
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IF 6.7
Mingqi Yang,Jiali Hu,Zhenlin Zhang,Liting Huang,Yahan Yu,Dexing Qiu,Yexin Zu,Yanyan Liu,Zhenyu Lin
Infection with hepatitis B virus (HBV) represents a major challenge to global health, especially in areas of inadequate healthcare infrastructure, rapid and on-site detection plays a crucial role in effective disease management and control. Consequently, it is necessary to develop some simple but sensitive HBV screening techniques. In this work, a homogeneous electrochemical biosensor was constructed for sensitive and specific HBV DNA detection through the integration of CRISPR/Cas12a target recognition and magnetic bead-based separation. The sensor exploits the collateral cleavage activity of CRISPR/Cas12a protein upon recognition of target double-stranded DNA, enabling the degradation of methylene blue-labeled single-stranded DNA captured on magnetic beads (MBs). Then the MBs are separated and redissolved in the solution and the electrochemical response of the system can be tested in homogeneous solution. The electrochemical signal has relationship with the concentration of HBV DNA within a range of 10 fM - 10 nM, and the LOD is 3.74 fM. The sensor demonstrates excellent selectivity and biological stability, which has potential application in clinical diagnosis, especially in resource-limited environments.
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