ACS Nano
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2024
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IF 15.8
Tao Sun,Huaishu Huo,Yingying Zhang,Yaru Xie,Yize Li,Kungang Pan,Fenfang Zhang,Jing Liu,Yindong Tong,Weiwen Zhang,Lei Chen
The pollution caused by heavy metals (HMs) represents a global concern due to their serious environmental threat. Photosynthetic cyanobacteria have a natural niche and the ability to remediate HMs such as cadmium. However, their practical application is hindered by a low tolerance to HMs and issues related to recycling. In response to these challenges, this study focuses on the development and evaluation of engineered cyanobacteria-based living materials for HMs bioremediation. Genes encoding phytochelatins (PCSs) and metallothioneins (MTs) were introduced into the model cyanobacterium Synechocystis sp. PCC 6803, creating PM/6803. The strain exhibited improved tolerance to multiple HMs and effectively removed a combination of Cd2+, Zn2+, and Cu2+. Using Cd2+ as a representative, PM/6803 achieved a bioremediation rate of approximately 21 μg of Cd2+/OD750 under the given test conditions. To facilitate its controllable application, PM/6803 was encapsulated using sodium alginate-based hydrogels (PM/6803@SA) to create “living materials” with different shapes. This system was feasible, biocompatible, and effective for removing Cd2+ under simulated conditions of zebrafish and mice models. Briefly, in vitro application of PM/6803@SA efficiently rescued zebrafish from polluted water containing Cd2+, while in vivo use of PM/6803@SA significantly decreased the Cd2+ content in mice bodies and restored their active behavior. The study offers feasible strategies for HMs bioremediation using the interesting biomaterials of engineered cyanobacteria both in vitro and in vivo.
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Journal of Energy Storage
·
2025
·
IF 9.8
Jin-hua Zhang,Peng-jie Zhu,Yan-zhen Liu,Xiang-ru Zhu,Yong-feng Li,Cheng-Meng Chen
The construction of heterostructured energy storage and conversion with special morphology has the potential to be an up-and-coming area of research. In this study, we designed and synthesized a Co₃O₄/g-C₃N₄ heterostructured electrode material with a distinctive morphology using a multistep fabrication approach. The heterojunction formed between Co₃O₄ and g-C₃N₄ induces interfacial charge redistribution, resulting in a built-in electric field that facilitates efficient electron transfer and enhances the electronic structure. Furthermore, the engineered morphology, comprising nanocages and hollow tubes, significantly increases the number of electrochemically active sites. The optimized electrode achieved an impressive specific capacity of 921.8 F g −1 at a current density of 1 A g −1 , along with excellent cycling stability, retaining 82.88 % of its initial capacity after 10,000 cycles. Moreover, an asymmetric supercapacitor constructed with Co₃O₄/g-C₃N₄ as the positive electrode and activated carbon as the negative electrode demonstrated a stable and high energy density of 36.3 Wh kg −1 .
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Deyang Ning,Junshan Zhang,Arun Murali,Yuanpei Lan,Chaoyi Chen,Shaoyan Yang,Wei Zhang,Junqi Li
In this work, a novel nitrogen-doped reduced graphene oxide-ceria (N-doped rGO-CeO 2 ) photocatalyst was successfully synthesized and exhibited a notable capability in degrading various organic pollutants . Among the synthesized samples, rGO-CeO 2 -900 N 2 demonstrated the most efficient photocatalytic activity , achieving a 77.43% degradation rate for tetracycline (TC) fitting the modified Elovich model ( R 2 = 0.99). The CeO 2 addition can increase defects and amorphous carbon species in rGO in Raman spectra . The rGO adding and N doping can contribute to more effective separation of photo-excited electrons/holes ( e - /h + ) and a faster rate of charge transfer via the results of photochemical tests. With the combination between rGO and CeO 2 , the N doped rGO-CeO 2 catalyst gains certain anti-H + ability in TC degradation and more·O 2 - can be generated under light, while the N doping can form an impurity level N 2p in CeO 2 and create additional reactive sites on the rGO. This N doped rGO-CeO 2 photocatalyst is well-suited for the harmless degradation and offers significant potential in wastewater treatment and pollution control.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
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IF 4.9
Huiyuan Xie,Yi Tang,Lidan Xie,Xueqin Chen,Mude Zhu,Yi Liu,Jiabin Wang,Xiaoyun Fan
How to promote the free charges of piezomaterials to take the redox is now a challenging work in environmental remediation. Construction of built internal electric fields is crucial to realize efficient charge separation for charge-induced redox reaction. Herein, PbB 4 O 7 (PBO) with lone pair electrons firstly realizes a high piezocatalytic degradation efficiency for removal of dye & Cr(VI) under ultrasound activation. PBO shows more excellent degradation performance than traditional BaTiO 3 (BTO), which is 2.08 times, 2.47 times, 2.28 times, and 3.02 times than that of BTO for RhB in the unary system, RhB & Cr(VI) in binary systems, MB in the unary system, and MB & Cr(VI) in binary systems, respectively. Piezoresponse force microscopy confirmed that PBO had excellent piezoelectric property. The varied degradation performance can be assigned to systems exposed to different ions (negative or positive) environment that influence shielding effect. Additionally, the easily polarization of lone pair electrons of the Pb-contained piezomaterial forming a strong alternating built-in electric field under ultrasound that can offer a strong driven force to separate electron and hole pairs, thus producing the superior piezocatalytic activities. The main active species in the unary system and the binary system were determined, and the piezocatalytic degradation mechanism of PBO was analyzed.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Yan Zhang,Yuyang Liu,Wannan Li,Weiwei Han
Antioxidant peptides can be obtained from diverse dietary protein sources with high safety. The Kelch-like ECH-associated protein 1(Keap1) is a key protein in the cellular oxidative stress signaling pathway and it plays an important role in the field of antioxidant. Soybeans are a vital source for the production of bioactive peptides. In this study, firstly, we screened 468 antioxidant peptides from soybean protein with low molecular weight, high hydrophobicity and non-toxicity. The top three antioxidant peptides were isolated and extracted from the fractions using UHPLC-QQQ/MS analysis. The free radical scavenging rate demonstrated significant antioxidant activity of PHHADS (EC 50 = 4.26 mmol/L). In addition, peptide PHHADS was demonstrated significantly protects HepG2 cells from oxidative stress-induced damage. Lastly, 200 ns molecular dynamics simulations were performed between the peptides and Keap1. The results revealed that PHHADS closely interacts with the active site of Keap1. Moreover, the interaction of the antioxidant peptides with Keap1 led to the disruption of the β-sheet structure of Keap1 in residues380-390, transforming into an irregularly curved structure. This particular region in Keap1 is crucial for substrates binding, suggesting a potential inhibitory mechanism by the peptides. These findings contribute to the understanding of antioxidant peptide interactions and their potential application in developing functional foods for health benefits.
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ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Jun-Yi Zhou,Yan-Qing Chen,Guang Hu,Haiyu Zhao,Jian-Bo Wan
Background N-acylethanolamines (NAEs) are a class of naturally occurring bioactive lipids that play crucial roles in various physiological processes, particularly exhibiting neuroprotective and anti-inflammatory properties. However, the comprehensive profiling of endogenous NAEs in complex biological matrices is challenging due to their low abundance, structural similarity and the limited availability of commercial standards. Here, we propose an integrated strategy for comprehensive profiling of NAEs that combines chemical derivatization and a three-dimensional (3D) prediction model based on quantitative structure-retention time relationship (QSRR) using liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-HRMS). Results After acetyl chloride (ACC) derivatization, the detection sensitivity of NAEs was significantly improved. We developed a QSRR prediction model to construct an in-house database for 141 NAEs, encompassing information on RT, MS 1 ( m/z ), and MS/MS spectra. Propargylamine-labeled fatty acids were synthesized as RT calibrants across various analytical conditions to enhance the robustness of the RT prediction model. NAEs in biological samples were then in-depth profiled using parallel reaction monitoring (PRM) acquisition. This integrated strategy identified and annotated a total of 50 NAEs across serum, hippocampus and cortex tissues from a 5xFAD mouse model of Alzheimer's disease (AD). Notably, the levels of polyunsaturated NAEs, particularly NAE 20:5 and NAE 22:6, were significantly decreased in 5xFAD mice compared to WT mice, as confirmed by accurate quantitation using ACC- d 0 / d 3 derivatization. Significance Our integrated strategy exhibits great potential for the in-depth profiling of NAEs in complex biological samples, facilitating the elucidation of NAE functions in diverse physiological and pathological processes.
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Foods
·
2025
·Vol. 14
·期 5
·763
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IF 5.1
Junye Zhu,Yuebin Zhou,Haitao Wen
At present, there are few studies on seasonal differences in the aroma quality and volatile components of Rucheng Baimao (Camellia pubescens) black tea. In this study, sensory evaluation and volatile component analysis were carried out on one sample of Rucheng Baimao black tea corresponding to spring, summer, and autumn, respectively. The results of sensory evaluation showed that the black teas of all three seasons had floral aromas. However, the aroma quality of spring black tea was the best, followed by that of autumn black tea, and summer black tea was the worst. The analysis of volatile components showed that alcohols, esters, and alkanes were the main substance categories. In addition, the results of the aroma index were consistent with those of the sensory evaluation, indicating that spring black tea had the best aroma quality, followed by autumn black tea and then summer black tea. Eleven key differential volatile components were screened by combining PLS-DA analysis (VIP > 1,p< 0.05) and rOAV > 1. Among them, geraniol, methyl salicylate, nonanal, and (E)-citral accumulated the most in spring black tea, linalool, phenylacetaldehyde, benzaldehyde, phenethyl alcohol, benzyl alcohol, andβ-ionone accumulated the most in summer black tea, andtrans-nerolidol accumulated the most in autumn black tea. This study aims to provide a theoretical reference for the regulation of the aroma quality of Rucheng Baimao black tea.
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Journal of Materials Chemistry A
·
2024
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IF 10.7
Ling Lu,Rundong Zhao,Huang Chen,Liang Tan,Dongxu Chen,Le Liu,Jingyu Xi
The nitrate reduction reaction (NO3RR) is a promising method for ammonia production, but detecting intermediate NO2− in situ during the reaction is challenging. We propose a nitrite concentration sensor based on equivalent absorbance change, comprising a light, absorption, and imaging module, with a linear detection range of 0–1.4 mg L−1. After optimizing the absorption module parameters and applying machine learning noise reduction, the detection limit is 0.0116 mg L−1. Using foam copper as the working electrode, we achieved real-time monitoring of nitrite concentration on the electrode surface during NO3RR, validating the sensor's design. When applied to Co Base/Cu electrodes synthesized via localized electrodeposition, the sensor shows significantly lower nitrite concentration in the electrodeposition area compared to the non-electrodeposition area during NO3RR, indicating superior nitrite utilization by cobalt-based materials. This sensor offers a new approach for investigating NO3RR mechanisms and designing catalysts.
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DRUG DELIVERY
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2012
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IF 1.456
Ning Li,Chun-Yang Zhuang,Mi Wang,Cheng-Guang Sui,Wei-San Pan
In this study, low molecular weight chitosan coated liposomes (LCHL) were designed and prepared for ocular drug delivery, the coating mechanism was studied, and in vitro and in vivo characterization was conducted. The effects of molecular weight and concentration of low molecular weight chitosan on the liposomal coating were studied. The numeric relations between coating variables and coating efficiency were established using a mathematical model. Morphology of LCHL was examined by transmission electron microscopy (TEM). Cytotoxicity and cell internalization of FITC-BSA labeled LCHL in a rabbit conjunctival epithelium (RCE) cell line were studied. Cyclosporin A (CsA) was encapsulated as a model drug, and in vitro drug release and in vivo drug absorption were investigated. LCHL demonstrated low toxicity to RCE cells. In vitro drug release measurement showed that LCHL had a delayed release profile compared with non-coated liposomes. In vivo study in rabbits showed that the concentrations of CsA in cornea, conjunctiva, and sclera were remarkably increased by LCHL. In conclusion, LCHL might be a potential ocular drug carrier with characteristics such as prolonged drug retention, enhanced drug permeation, and biocompatibility.
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ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Qi Guo,Xiaofeng Yue,Xin Qi,Xinyao Feng,Xuefang Wang,Xiaofeng Hu,Fei Ma,Liangxiao Zhang,Peiwu Li,Li Yu
The aim of this survey was to evaluate the residue levels, distribution and exposure risk of the 38 most commonly used pesticides in rapeseed samples collected from the main production areas in China over a two-year period. The sampling area covered 12 provinces, including Guizhou, Shaanxi, Yunnan, Hunan, Jiangxi, Sichuan, Chongqing, Anhui, Henan, Hubei, Zhejiang, and Jiangsu provinces. The pesticide residues were determined using a QuEChERS (Quick Easy Cheap Effective Rugged and Safe) method coupled with gas chromatography-tandem mass spectrometry and liquid chromatography-tandem mass spectrometry. 8.4% of the rapeseed samples contained pesticides with a residue level ranging from 0.001 to 0.634 mg/kg. The detected analytes were imidacloprid , quizalofop-P-ethyl, thiamethoxam , paclobutrazol , prochloraz, tebuconazole , difenoconazole, s-metolachlor, carbofuran , and carbendazim . The concentrations of four analytes, including thiamethoxam, difenoconazole, carbendazim and prochloraz, exceeded the maximum residue level set by the Chinese government for rapeseed, with exceedance rates of 0.1%, 0.1%, 0.1%, and 1.1%, respectively. Based on the index of quality for residues (IqR) values, 91.6% of the total rapeseed samples had an IqR category of Excellent (IqR = 0). Only 1.5% of the tested samples were of inadequate quality. Furthermore, the assessment of chronic and acute exposure, as well as health risks associated with pesticide residues in rapeseed, was conducted for different age groups within the Chinese population, including adults (6–14 years), children (15–49 years), and the elderly (50–74 years). The results of this assessment indicated that pesticide residues in rapeseed cultivated in China are not expected to be of short- or long-term risks to the Chinese customers.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
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IF 3.3
Wanyu Yan,Cong Chen,Yuanru Zheng,Jingwen Xu,Yihai Wang,Xiangjiu He
BACKGROUND Apples are among the most nutritionally valuable fruits and have a history of use in traditional Chinese medicine. Triterpenoids, the primary bioactive compounds found in apples, demonstrate significant antitumor activity. RESULTS Following enrichment and optimization, the total content of major triterpenoids in total triterpenoids from apple peels (ATT) reached 5.76 g kg −1 . The growth of MDA-MB-231 xenograft tumors was significantly inhibited after treatment with ATT. Network pharmacology analysis conclusively identified a close association between the antitumor effect of ATT and the phosphatidylinositol 3-kinase–protein kinase B (PI3K-Akt) signaling pathway. Experimental validation using MDA-MB-231 cells and a xenograft nude mouse model confirmed that ATT suppressed tumor cell proliferation effectively by modulating the PI3K-Akt signaling pathway, which was consistent with the findings from network pharmacology. The total triterpenoids from apple peels also induced cell apoptosis by mediating the PI3K-Akt signaling pathway. CONCLUSION The total triterpenoids from apple peels can inhibit tumor cell proliferation and induce cell apoptosis effectively through the PI3K-Akt signaling pathway, suggesting that ATT holds promise as a prospective therapeutic agent for breast cancer treatment. © 2024 Society of Chemical Industry.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-17
·
IF 10.6
Liu Ye,Chen Xu,Chen Xinghao,Chen Jie,Zhang Han,Xu Haonan,Jin Lu,Wang Qiao,Tang Zhan
Sirolimus (SR) is a macrolide with antifungal and antitumor immunosuppressant properties, classified as a selective inhibitor of mammalian target of rapamycin (mTOR). In this study, an ionic in situ gel of SR (SR-SUS-ISG) was formulated using gellan gum, exhibiting stability regardless of temperature and pH variations, causing minimal irritation. Harnessing the physiological conditions of the eye, SR-SUS-ISG underwent gelation upon contact with ions, increasing drug viscosity and prolonging retention on the ocular surface. Concurrently, SR-SUS-ISG displayed favorable shear dilution properties, reducing viscosity at ambient temperature, enhancing fluidity, and facilitating convenient packaging and transport. Biocompatibility assessments on both human corneal epithelial cells and rabbit eyes demonstrated that SR-SUS-ISG could well be tolerated. Pharmacokinetic investigations in rabbit ocular aqueous humor revealed sustained release, improved corneal penetration, and enhanced bioavailability. Additionally, in a rat corneal alkali burn model, SR-SUS-ISG exhibited inhibitory effects on corneal neovascularization, associated with decreased levels of the inflammatory factors VEGF and MMPs. These findings suggested that SR-SUS-ISG held promise as an effective ocular drug delivery system. Graphical Abstract
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危险品化学品经营许可证（带存储）