DIAMOND AND RELATED MATERIALS
·
2025
·
IF 5.1
Jun Liu,Jiayin Li,Yao Chen,Xin Tan,Chun Yang
The abnormal contain of uric acid (UA) and tryptophan (Trp) in body could cause the liver and kidney function decline. The simultaneous detection of UA and Trp for early prevention of these liver and kidney diseases is significant. This study had developed the α-Fe 2 O 3 /reduced graphene oxide (rGO) for modification of the glassy carbon electrode (GCE) in (UA) and (Trp) detection. α-Fe 2 O 3 /rGO composite was synthesized through hydrothermal-calcination method. The optimal electrochemical conditions, including the pH values, scan rate, and accumulative parameters, were discussed for demonstrating the feasibility. Moreover, this α-Fe 2 O 3 /rGO/GCE presented wide linear relationships (0.01–900 μM) and low detection limits (UA (3.56 nM) and Trp (16.4 nM)). The DFT theoretical calculation revealed that the enhanced performance is mainly due to the high electrocatalytic property of α-Fe 2 O 3 nanotube, high conductivity of rGO, and interaction effect between target biomolecule and modified electrode surface. Finally, the approving results were also acquired in detection of practical UA and Trp samples by α-Fe 2 O 3 /rGO modified GCE. The results indicate that α-Fe 2 O 3 /rGO could provide a new and highly advantageous approach for detection of UA and Trp.
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ELECTROCHEMISTRY
·
2025
·
IF 1.7
Hongyu CHEN,Ru TIAN,Jianke LI,Beibei HAN,Guoku LIU,Shiyu YIN,Guiying XU,Kun WANG,Fukuan WANG,Baigang AN,Dongying JU,Weimin ZHOU
TiO2 as a semiconductor photocatalyst has been extensively utilized in the purifications of water and air. However, the wide band gap of TiO2 necessitates its activation by ultraviolet irradiation, when it is utilized as a photocatalyst. To enhance the photocatalyst performance of TiO2, constructing the heterojunction containing TiO2 is considered as a highly effective method. To unfold the actual application, iron metal oxides are firstly utilized to construct the heterojunction of TiO2/α-Fe2O3, and the synthesized TiO2/α-Fe2O3 heterojunction was successfully coated on the surface of coal tar pitch based spherical activated carbons (CTP-SACs). The structures of TiO2/α-Fe2O3/CTP-SACs are verified by the X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The results of UV-vis absorption spectroscopy supports that TiO2/α-Fe2O3/CTP-SACs are promising candidates for the natural light photoncatalysis. It is believed that the exceptional conductivity of CTP-SACs enhances the activity of the photocatalyst, resulting in the TiO2/α-Fe2O3/CTP-SACs exhibiting remarkable sterilization effects under natural light. For instance, the TiO2/α-Fe2O3-60-CTP-SAC shows an excellent sterilization rate (89 %) for the fish culture water under the irradiation of natural light for 20 min. Associating with the excellent liquidity of CTP-SACs, it is considerable that TiO2/α-Fe2O3/CTP-SACs possess the captivating application prospects in a field of the feeding water treatment.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Yu Wang,Qilin Zhao,Qi Fang,Jian Sun,Yan Du,Haji Akber Aisa
Enzyme cascades have attracted widespread attention owing to the exceptional specificity and efficient signals transduction, however, constrained by the high cost and limited stability of bio-enzymes. In this study, a novel mimic multienzyme nanoreactor (GOx@MAF-7(Fe), i.e. GMF) was developed through a one-step encapsulation of glucose oxidase (GOx) into a metal azolate framework, MAF-7(Fe). Benefiting from the synergistic effect of GOx and the exceptional peroxidase-like (POD) activity of MAF-7(Fe), GMF enabled a robust cascade catalytic reaction for colorimetric sensing. The unique structural and functional properties of MAF-7(Fe) not only facilitated efficient enzyme immobilization but also enhanced the stability of GOx, outperforming free enzymes in terms of storage and thermal tolerance. The GMF-based platform demonstrated high sensitivity and selectivity in glucose response. More importantly, by integrating α-glucosidase (α-Glu) into a three-enzyme cascade system, a colorimetric assay was successfully developed for α-Glu activity with a detection limit of 0.25 mU/mL, surpassing most existing methods. This platform was further applied for α-Glu inhibitor screening, with acarbose as a model inhibitor, and achieved precise quantification of inhibition efficiency (IC 50 = 60.06 nM). This work not only establishes a versatile and efficient sensing platform for diabetes-related biomolecule detection but also pioneers a novel strategy for enzyme immobilization and multienzyme cascade construction, opening new avenues for multifunctional material design in biomedical research.
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Xiangxuan Feng,Yaohui Wang,Yifan Wang,Jun Ren
The dysregulated overproduction of pro-inflammatory cytokines like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) is a hallmark of severe inflammatory diseases. Extracorporeal blood purification offers a promising therapeutic strategy, but current adsorbents often lack the specificity required for precise intervention. This study reports the development of a bispecific nanobody (bsNb)-based immunoadsorbent for the simultaneous and specific removal of IL-6 and TNF-α. The functional bsNb, produced in a prokaryotic system via tandem fusion, was covalently immobilized on Sepharose CL-6B matrix to prepare the affinity adsorbent. Comprehensive characterization confirmed its high saturation adsorption capacity (6.9 mg/mL for IL-6 and 2.2 mg/mL for TNF-α) and rapid binding kinetics. In a simulated hemoperfusion system using human serum, the adsorbent achieved removal efficiencies exceeding 85% for both cytokines across a range of pathophysiological concentrations. Crucially, the bsNb adsorbent effectively captured both targets simultaneously from a complex serum matrix. This work highlights the significant potential of bispecific nanobodies as robust and selective ligands for preparing targeted immunoadsorbents. The developed platform represents a superior alternative to non-specific adsorbents, paving the way for more precise and effective extracorporeal therapies against cytokine-driven inflammatory disorders.
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PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Huabiao Miao,Rui Jiang,Nanyu Han,Yu Ma,Qian Wu,Yuelin Mu,Zunxi Huang
Amylases have been widely applied for starch saccharification in the food, paper, pharmaceutical, and textile industries. In the present study, α-amylase DL-3-4−1 from B. amyloliquefaciens was successfully expressed in B. subtilis. The enzymatic activity exhibited high stability in a pH range from 4.0 to 11.0 and moderate thermostability. Furthermore, excellent stability was observed when incubated with metal ions, detergents, inhibitors, and organic solvents. Resistance against ethanol, urea, and guanidine−HCl was also observed. More importantly, a strategy to enhance the extracellular expression of α-amylase DL-3-4−1 was developed using 173 Sec-type signal peptides (SPs) as a library based on a fast and sequence-independent method. Fourteen SPs were identified with significantly increased yields of DL-3-4−1 via a high-throughput assay. Specifically, the enzymatic activity of the α-amylase-producing strain containing the signal peptide SP5D10 (YomL) was 3.74-fold higher than that of the strain with the wild type signal peptide (87.30 U/OD). In brief, the maximum specific activity of an α-amylase-producing strain containing the YomL signal peptide was 326.45 U/OD. This is the first report of an α-amylase that exhibits ethanol, urea, and guanidine−HCl tolerance. Hence, this α-amylase represents a promising candidate for future use in industry.
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高影响因子
Molecular Medicine Reports
·
2019
·Vol. 20
·期 1
·332-340
·
IF 1.851
Lina Liu,Jing Yan,Fei Ge,Xiangtao Xu,Jun Lu,Huilian Shi,Shuihong Li,Yue Zhao,Changzheng Zhang
Saikosaponin‑D (SSD), which is the main bioactive component in the traditional Chinese medicine Chai Hu (Bupleurum falcatum L), possesses estrogen‑like properties and is widely used in treating estrogen‑related neurological disorders. The current study aimed to investigate the protective effects of SSD on the fear memory deficit in ovariectomized (OVX) rats and the potential underlying mechanism. SSD treatment significantly prolonged freezing time in OVX rats in a manner similar to that of estradiol (E2), whereas this effect was markedly suppressed by co‑administration of ICI182780, a non‑selective estrogen receptor (ER) inhibitor. The expression of ERα in the hippocampus of OVX rats was significantly elevated by SSD; however, Erβ expression and E2 synthesis were not markedly affected by SSD treatment. Collectively, this study demonstrated that SSD‑mediated fear memory improvement in OVX rats may be attributed not to E2 levels or ERβ activity, but to ERα activation in the hippocampus.
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PubMed
高影响因子
Toxins
·
2025
·Vol. 18
·期 1
·7
·
IF 4
Cheng Chen,Lu Chen,Hongyuan Zhou,Xiao Li Shen,Liang Ma
α-Zearalenol (α-ZOL), the primary metabolite of zearalenone (ZEN), is a prevalent mycotoxin in agricultural products (e.g., corn, wheat) and poses health risks due to its toxicity. However, strategies to mitigate its toxicity are needed. Therefore, this study aims to determine whether selected polyphenols (quercetin, baicalin, rosmarinic acid, naringenin) can competitively displace α-ZOL from human serum albumin (HSA) and to clarify the interaction mechanisms. The results showed that competitive interactions between α-ZOL, HSA, and the polyphenols were observed. The polyphenols bound HSA more tightly than α-ZOL (higherKa) and significantly reduced α-ZOL’sKa, indicating direct competition. Moreover, as evidenced by synchronous fluorescence, the polyphenols altered the microenvironments of tyrosine and tryptophan residues, directly impacting α-ZOL binding. The HPLC-ultrafiltration results revealed that the polyphenols tested competitively displaced α-ZOL from HSA, with the relative potency of quercetin ≈ baicalin > rosmarinic acid > naringenin. Collectively, our competitive binding assays demonstrate that quercetin, baicalin, rosmarinic acid, and naringenin competitively displace α-ZOL from its binding site(s) on HSA. Thus, our study not only suggests a novel mechanism to alleviate the toxicity of ZEN and α-ZOL but also provides a scientific basis for developing dietary interventions against these mycotoxins.
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高影响因子
ChemCatChem
·
2021
·Vol. 14
·期 3
·e202101489
·
IF 5.686
Lingling Peng,Wansen Tan,Yuting Lu,Ayan Yao,Dayuan Zheng,Le Li,Jingran Xiao,Lijun Li,Qingbiao Li,Shu-feng Zhou,Guowu Zhan
Graphical Immobilized Rha on MOFs : Herein, we explored five typical metal-organic frameworks (MOFs) as porous carriers to immobilize Rha, and the obtained Rha@Ce-BTC showed the highest enzymatic activity. Also, Rha@Ce-BTC manifested enhanced enzyme-substrate affinity, great reusability/storage stability, and catalytic efficiency compared to free Rha. Significantly, the as-prepared Rha@Ce-BTC might be assembled as a membrane reactor for efficient biocatalysis reactions. The α-L-Rhamnosidase (Rha) is a useful glycoside hydrolase for selectively hydrolyzing the terminal L-rhamnose residues in flavonoids, being vital to food and pharmaceutical industries. However, Rha suffers from low recyclability and poor stability in harsh environments. Herein, we explored five typical metal-organic frameworks (MOFs) as porous carriers to immobilize Rha, and the activities of the resultant Rha@MOF composites were compared with the free enzyme. The locations of the enzyme in MOFs were proved by a series of characterization techniques. It was found that Rha@Ce-BTC (with enzyme immobilization efficiency of 23 % and enzyme loading content of 8.8 %) showed the highest enzymatic activity. The immobilized Rha@Ce-BTC showed 80 % residual activity after five consecutive cycles, suggesting a limited leaching effect. Also, Rha@Ce-BTC manifested markedly enhanced enzyme-substrate affinity and catalytic efficiency compared to free Rha, as supported by Michaelis-Menten kinetic studies. Accordingly, the Ce-BTC would be an appealing carrier for enzyme immobilization, and the as-designed enzyme/Ce-BTC composites are promising candidates for industrial use with remarkably high activity, recyclability, and storage stability.
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高影响因子
FUEL
·
2022
·
IF 8.035
Jin Zhang,Mengting Gao,Pengqi Zhu,Yunwei Wang,Ruiyi Wang,Zhanfeng Zheng
The design of efficient photocatalyst for selective hydrogenation of α,β -unsaturated aldehydes to unsaturated alcohols is changeling. In this study, oxygen vacancy enriched MgAl-layered double hydroxide (MgAl-LDH) supported Co 3 O 4 nanoparticle photocatalysts are developed taking the advantage of the semi-memory effect of CoMgAl-LDH. Excellent photocatalytic performance was achieved with a 99 % cinnamaldehyde conversion and 98 % cinnamyl alcohol selectivity at 100 °C under visible light irradiation. The electron enriched Co 3 O 4 nanoparticles facilitated the activation of isopropanol to provide active H species under light irradiation. In situ DRIFTS and DFT calculation results suggest the oxygen vacancies of MgAl-LDH were beneficial to the terminal O adsorption of –C O group in cinnamaldehyde and ensure the high selectivity of cinnamyl alcohol. This study demonstrated a novel synthesis strategy of Co 3 O 4 nano-photocatalyst for the efficient selective hydrogenation under visible light irradiation.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Qin Hu,Shengtao Zhang,Feng Chen,Wenpo Li,Hao Jiangyu,Xinyue Liang,Bin Xiang,Chao Chen,Xuefeng Zou
Nickel sulfide possesses ultra-high theoretical energy storage capacity. Though it is easily obtained, it is very difficult to exert its intrinsic strong capacity. In this work, a new strategy based on a binary synergy of sulfur sources is introduced. By regulating the molar ratio of two sulfur sources, a high-performance α-NiS/Ni 3 S 4 binary hybrid is successfully synthesized. Interestingly, it is found that changing the molar ratio of two sulfur sources in hydrothermal process can efficiently regulate the components of product but cannot visibly affect its morphology. The electrochemical results indicate that this strategy is highly effective for improving the performance of nickel sulfide. As a result, a highest specific capacity of 214.9 mAh g −1 at 2 A g −1 was reached. In addition, the fabricated S3//rGO hybrid supercapacitor displays a highest energy density of 41.9 Wh kg −1 at a power density of 799.0 kW kg −1 . Moreover, the device delivers an excellent cycle stability with 103% capacity retention rate after 10,000 cycles. These findings open a new avenue for the controlled synthesis of high-performance nickel sulfides or other metal sulfides.
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JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2013
·
IF 2.823
Long-Hua Zhou,Na Wang,Wei Zhang,Zong-Bo Xie,Xiao-Qi Yu
An facile and green one-pot route has been developed for the synthesis of chromenes using salicylaldehyde and α,β-unsaturated ketones. α-Amylase from Bacillus subtilis shows excellent catalytic activity and exerts good adaptability to different substrates in the reaction. This promiscuous enzyme-catalyzed domino reaction not only extends the application of α-amylase from B. subtilis for new chemical transformations, but also provided an alternative synthetic method for 2 H -chromene derivatives.
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高影响因子
ACS Omega
·
2021
·
IF 3.512
Lin-Xi Wan,Shi-Xing Miao,Zhen-Xiang He,Xiaohuan Li,Xian-Li Zhou,Feng Gao
Palladium/BuAd2P efficiently catalyzed the direct α-arylation of ketone in the anti-Alzheimer’s disease drug donepezil, leading to 15 aryldonepezil analogues exhibiting high selective inhibition of acetylcholinesterase (AChE). The cell-based assays revealed that the 3-methylpridinyl analogue (12) shows significantly lower toxicity compared to donepezil and remarkable neuroprotective activity against H2O2-induced damage in SH-SY5Y cells. Docking results of compound 12 also interpreted the possible mechanism of the selective inhibition between AChE and butyrylcholinesterase (BuChE).
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