CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Lingli Wang,Yi Tang,Pu Wang,Yu Fu,Chunxiao Xu,Zhaohui Wang
Although thermally activated peroxydisulfate (PDS) is often regarded as a radical-dominant oxidation process, the accurate temperature threshold necessary for substantial radical production remains a subject of debate, despite its critical relevance for mechanism interpretations and process design optimization. Through a series of radical identification experiments (i.e., electron paramagnetic resonance (EPR) and fluorescence assays), we demonstrate that hydroxyl radical ( OH) and sulfate radical (SO 4 − ) are not genuinely produced at lower temperatures (25–47 °C). The detection of the 5,5-dimethyl-1-pyrroline N -oxide (DMPO) adduct of CH 2 OH (indicative of the H abstraction of methanol by free radicals) and hydroxylated product of the fluorescence probe coumarin, provides preliminary evidence that the temperature threshold for radical generation in the heat/PDS system is 48 °C. This threshold is further refined to 45 °C, as evidenced by the detection of hydrogen signals corresponding to the heptet of 5,5-dimethylpyrrolidin-2-one-1-oxyl (DMPOX) at this temperature, using 1 H nuclear magnetic resonance (NMR) technique. At a temperature of 80 °C, SO 4 − is initially generated in the heat/PDS system, resulting in the formation of the heptet DMPOX. In conclusion, this study clarifies the identity of SO 4 − and establishes the temperature threshold for its generation, which has significant implications for the process optimization and cost saving.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Si Mengyao,Han Guo,Liu Tianwen,Wang Xiaoying
Aberrant overexpression of c-Myc protein (c-Myc) serves as a critical biomarker for aggressive lymphomas, yet its trace-level quantification remains challenging due to insufficient sensitivity in conventional assays. Here, we present an electrochemical immunosensor leveraging homogeneous gold–palladium (AuPd) nanohybrids for dual amplification, achieving both simplified fabrication and enhanced analytical performance. A chitosan–graphene oxide–AuPd nanorod composite (CS-GO-AuPd) was engineered as an electrode modifier, offering high conductivity, biocompatibility, and abundant antibody immobilization sites. Prussian blue-doped AuPd nanoparticles (PB-AuPd@Ab 2 ) were simultaneously designed as catalytic signal probes, synergistically amplifying responses through electron mediation and nanozyme activity. This homogeneous material strategy eliminated heterogeneous nanomaterial interfaces, streamlining sensor assembly while ensuring signal reproducibility. The sandwich-type immunocomplex formation triggered a PB-derived differential pulse voltammetry signal proportional to c-Myc concentration, yielding a 0.5 pg mL −1 –1 ng mL −1 linear range, with a detection limit of 0.25 pg mL −1 . Comparative validation against clinical methods confirmed robust accuracy, highlighting the platform’s potential for dynamic monitoring of lymphoma progression and therapy response. This work establishes a paradigm for unifying material homogeneity with dual-amplification mechanisms in biosensing, extensible to diverse low-abundance biomarkers. Graphical
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Turkish Journal of Gastroenterology
·
2025
·Vol. 36
·期 9
·573
·
IF 1.6
Hailiang Liu,Xile Wei,Ye Nie,Jianshan Liu,Ming Fan
Background/Aims: The function and mechanism of ubiquitin-specific protease 34 (USP34) in hepatocellular carcinoma (HCC) were explored to provide new molecular targets for treating HCC. Materials and Methods: In the present study, bioinformatics techniques, quantitative real-time polymerase chain reaction, and western blot were used to detect the level of USP34 in HCC tissues and cell lines. Small interfering RNA of USP34 was transfected into HCC cells, and then Cell Counting Kit-8 (CCK-8) assay, wound healing assay, and transwell assay were performed to verify the proliferation, migration and invasion of HCC cells. Enzyme-linked immunosorbent assay was utilized to assess the glycolysis level in HCC cells. Co-immunoprecipitation was used to evaluate the ubiquitination level of cellular Myc (c-Myc). Results: The expression of USP34 was upregulated in HCC patients and strongly associated with worse outcomes. Meanwhile, interference with USP34 suppressed the proliferation, migration, and invasion of HCC cells. In addition, silencing of USP34 reduced the glucose uptake, lactate production and ATP content in HCC cells, as well as downregulated the expression levels of glycolysis-related proteins (hexokinase 2, glucose transporter 1, pyruvate kinase M2, and lactate dehydrogenase A). Furthermore, the knockdown of USP34 enhanced the ubiquitination level of c-Myc and increased the degradation of c-Myc. Overexpression of c-Myc reverted the inhibitory effects of si-USP34 on the malignant biological behavior in HCC cells. Conclusion: TheUSP34 regulates aerobic glycolysis and inhibits the progression of HCC by accelerating c-Myc ubiquitinated degradation.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Martin C. Koo,Yu Zhang,Bo Cai,Chen-Ming Liang,Shao-Hang Shi,Hua-Long Peng,Shu-Hao Yang,Xiao-Bo Sun,Guang-Sheng Wang
Microwave-absorbing materials (MAMs) play an important role in reducing electromagnetic pollution. However, achieving a balance between low filler loading, low minimum reflection loss (RL min ) and wide effective absorption bandwidth (EAB) for efficient absorption of microwaves remains challenging. In this work, an innovative method of using CO 2 atmosphere annealed Ti 3 C 2 T x MXene (C/TiO 2 ) combined with nickel nanowires (NiNW) via ultrasonic treatment was performed to yield C/TiO 2 /NiNW (CTN) composites with abundant NiNW/TiO 2 contacts. The electromagnetic (EM) parameters of the as-synthesized composites were closely studied through a series of experiments and computed theoretical calculations. Through the establishment of built-in electric fields (BIEFs) at the NiNW/TiO 2 interface, the derived CTN composite demonstrated improved dielectric properties and impedance matching characteristics that are clearly reflected on the microwave absorption performance of our as-synthesized MAMs, with RL min improving by 127.9% from −31.93 dB to −72.77 dB, and widest EAB increasing by 25.9% from 4.25 GHz to 5.35 GHz, at a low filler loading of 10 wt.%. This work investigates the mechanisms by which BIEFs influence EM parameters, providing critical insights into improving microwave absorption performance via BIEF structural design.
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Molecular Catalysis
·
2025
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IF 4.9
Qiuju Qin,Chaolian Zhu,Donghai Mo,Zhengjun Chen,Lihui Dong,Bin Li,Liya Zhou
MnO x exhibits excellent low temperature activity for degradation NO x , but its poor SO 2 tolerance restrains its application. Herein, an in situ construction method for MnO x loading on Mn-doped ZrO 2 carrier with carbon (MnO x /Mn-ZrO 2 -C) derived from Mn(NO 3 ) 2 /UIO-66 by in situ doped pyrolysis–oxidation, in which Mn replaces Zr to modify the electronic structure of the ZrO 2 . The MnO x /Mn-ZrO 2 -C with abundant surface oxygen and acid sites showed excellent activity and better SO 2 resistance. A series of characterization results indicated that Mn doping modulates the electron structure of carrier ZrO 2 and enhances the interactions among MnO x , carbon, and Mn-ZrO 2 , resulting in increasing the electron cloud density around Mn and Zr, and consequently, the Mn and Zr on the MnO x /Mn-ZrO 2 -C exhibit poorer sulfiphilic. These findings emphasize the benefits of utilizing a multipronged effect to fabricate highly active and sulfur-resistant NH 3 -SCR catalysts.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Liping Sun,Chao Li,Yong Yan,Yue Yu,Hao Zhao,Zijue Zhou,Feng Wang,Yi Feng
Single atom nanozymes (SAzymes) represent the state-of-the-art technology in nanomaterial-based catalysis, which have attracted attentions in catalysis, cancer treatment, disinfection and biosensing fields. However, numerous SAzymes suffered from low aqueous dispersion and without recognition capacity, which impeded their applications in bioanalysis . Herein, we engineered DNA onto SAzymes to obtain the DNA/SAzymes conjugates , which significantly improved the aqueous dispersion and recognition ability of SAzymes. We synthesized iron SAzymes (Fe–N–C SAzymes) as the catalytic nanomaterials, and investigated the interactions between Fe–N–C SAzymes and DNA. We compared A 15 , T 15 and C 15 adsorption of Fe–N–C SAzymes in HEPES containing 2 mM MgCl 2 . We found that 50 μg mL −1 Fe–N–C SAzymes produced nearly 100% A 15 adsorption, 90% T 15 adsorption and only 69% C 15 adsorption, indicating that adenine and thymine had higher adsorption affinity on Fe–N–C SAzymes. More importantly, DNA modification did not affect the peroxidase-like activity of Fe–N–C SAzymes and the bioactivity of the adsorbed DNA. Taking the advantage of the diblock DNA with one DNA sequence (adenine) binding to Fe–N–C SAzymes and the other DNA sequence (i.e., aptamer) binding to cancer cells, we designed Apt/Fe–N–C SAzymes for colorimetric detection of cancer cells, which offered new insights for the use of SAzymes in biomedicine .
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Advanced Healthcare Materials
·
2019
·Vol. 9
·期 1
·1901203
·
IF 6.27
Haiying Ji,Guangxi Wu,Yongyong Li,Kun Wang,Xiaomei Xue,Shasha You,Shengming Wu,Tianbin Ren,Bin He,Xueyin Shi
Elevated low-density lipoprotein cholesterol (LDL-C) increases the risk of atherosclerotic cardiovascular disease. Peptide-based PCSK9 vaccines have shown a promising prospect of reducing LDL-C. In peptide vaccine (pVax) design, the peptide antigens need to conjugate with carrier protein (CP). However, CP incorporation can induce undesirable anti-CP antibodies, which sterically mask peptide epitopes from being recognized by specific B cells and impair subsequent therapeutically antibody production. This epitopic suppression has posed a barrier in clinical translation of conjugate vaccines all along. A model CP (keyhole limpet hemocyanin, KLH) is herein camouflaged with serum albumin (SA) into hybrid nanocarriers (SA@N), with PCSK9 peptide being anchored onto the surface to form nanovaccine (SA@NVax). Such camouflage of KLH via high “self” SA coverage is able to inhibit KLH from extracellular immune recognition and prevent detectable anti-KLH antibody production. Furthermore, the nanovaccine around 70 nm stabilized by intermolecular disulfide network is ideal for internalization and biodegradation by antigen presenting cells as well as better retention in draining lymph nodes and spleen. As expected, the SA@NVax efficiently primes higher anti-PCSK9 IgG antibody titer than PCSK9 pVax.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
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IF 3.9
Mengxue Li,Juanjuan Wang,Nana Zhu,Yang Zong,Rui Jiao,Zhaoqi Zhu,Hanxue Sun,An Li
Fe–N–C catalysts are some of the most promising catalysts for the oxygen reduction reaction (ORR). However, there are still some problems in the pyrolysis of precursors, such as the destruction of the carbon skeleton and low utilization of active sites. In this work, we synthesized hollow pyridine N-containing conjugated microporous polymers (CMPs) by coupling polymerization. The bipyridine units in CMPs’ skeleton can anchor Fe3+ to form a stable coordination structure, which was effectively converted into Fe–N–C catalysts by one-step pyrolysis. Due to the rigid skeleton of precursors, the hollow structure was preserved in catalysts, which is favorable to O2 diffusion and mass transport of intermediates. X-ray photoelectron spectroscopy and Fe K-edge X-ray near-edge spectroscopy revealed approximate FeN4 structures within the framework of the catalysts. As a result, the resulting CMP-derived catalysts exhibited satisfactory ORR activity in alkaline media with a limiting current density of 4.40 mA cm–2. Furthermore, the Fe,N–C1–900 catalyst demonstrated exceptional methanol immunity with a mere negative shift of 0.01 mA cm–2 in limiting current density compared with the commercial Pt/C catalyst, indicating remarkable stability.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yingying Zheng,Weihao Dai,Jian Wang,Yifei Zhang,Biyun Peng,Fangfang Wu,Yunchuan Xie,Sen Liang,Di Zhou
The fatal rise in electrical conduction at elevated temperatures (> 150 °C) fundamentally limits polymer dielectrics, collapsing both energy density ( U d ) and efficiency ( η ) through thermally activated π-electron delocalization. While conventional crosslinking stiffens matrices, it neglects the electronic-structural origin of leakage: continuous π-orbital overlap/π-conjugation pathways along polymer backbones. Here, we report a spatial-crosslinked molecular-level strategy to surgically disrupt conjugation pathways in polyimides. By incorporating phenylethynylphthalic anhydride (PEPA) as a spatial-conformational modulator, we force critical dihedral rotations between phenyl rings through steric repulsion. This intentionally distorts chain geometry, fragmenting extended π-networks into isolated segments, concurrently improving mechanical stiffness and intrinsically suppressing conduction loss (maintaining high η ). The synergy enables outstanding U d (4.89 J cm −3 at 150 °C; 2.11 J cm −3 at 200 °C) with η > 90 %, exceeding conventional PIs' limits at 200 °C. These films also show exceptional stability (> 10 4 cycles) and solution processability for scalable fabrication. By addressing the electronic-structural root of conduction loss, this work establishes spatial-crosslinking as a general design paradigm for next-generation high-temperature dielectric polymers.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Youyou Feng,Jing Qin,Yu Zhou,Qin Yue,Jing Wei
Nanozyme has been regarded as an efficient antibiotic to kill bacteria using the reactive oxygen species (ROS) generated by Fenton-like reaction. However, its activity is still unsatisfied and requires large amount of hydrogen peroxide with side effects toward normal tissues. Herein, spherical mesoporous Fe-N-C single-atom nanozyme (SAzyme) is designed for antibacterial therapy via photothermal treatment enhanced Fenton-like catalysis process. Due to the large pore size (4.0 nm), high specific surface area (413.9 m 2 g −1 ) and uniform diameter (100 nm), the catalytic performance of Fe-N-C SAzyme is greatly improved. The Michaelis-Menten constant ( K m ) is 4.84 mmol L −1 , which is similar with that of horseradish peroxidase (3.7 mmol L −1 ). Moreover, mesoporous Fe-N-C SAzyme shows high photothermal conversion efficiency (23.3 %) owing to the carbon framework. The catalytic activity can be enhanced under light irradiation due to the elevated reaction temperature. The bacteria can also be killed via physical heat effect. Due to the synergistic effect of nanozyme catalysis and photothermal treatment, the antibacterial performance is much higher than that using single antibacterial method. This work provides an alternative for combined antibacterial treatment via photothermal treatment assisted catalytic process using spherical mesoporous single-atom nanozyme as an antibiotic.
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MOLECULES
·
2025
·Vol. 30
·期 23
·4583
·
IF 4.6
Ting Zhang,Jiale Song,Zhenzhen Peng,Mengjiao Wu,Zhi Li,Fei Li,Yuxi Wei
Polysaccharides from marine organisms have been extensively studied and utilized as functional food ingredients due to their excellent immunomodulatory properties. However, the immunomodulatory potential of fermentedRuditapes philippinarumpolysaccharide (RPP) has not been systematically explored. This study investigated the effects of RPP on immune function in cyclophosphamide (CTX)-induced immunosuppressedBALB/cmice. These results revealed that RPP alleviated CTX-induced weight loss and restored appetite. Moreover, RPP can promote the morphology and indices of immune organs, as well as increased the number of white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb). Serum levels of interleukin-6 (IL-6) and immunoglobulin E (IgE) were significantly elevated following RPP treatment. Additionally, RPP improved colonic morphology by upregulating the expression of E-cadherin and ZO-1 and promoting the secretion of secretory IgA (sIgA). These results indicated that RPP exerted an immune protective effect inBALB/cmice and justified its further potential as a bioactive ingredient for functional foods derived from marine shellfish.
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Nanomaterials
·
2025
·Vol. 16
·期 1
·35
·
IF 4.3
Wenjing Wu,Zichao Yang,Min Zhang,Zhongjie Guan,Jianjun Yang
Photocatalytic reduction of carbon dioxide is a very effective strategy to address the energy crisis and greenhouse effect. TiO2is a widely used semiconductor photocatalyst, which has excellent catalytic activity, excellent chemical stability and low toxicity. Nevertheless, TiO2still has some inherent limitations, such as: wide band gap, high carrier recombination rate, and low adsorption activation ability for carbon dioxide. These drawbacks severely restrict its further application in the photocatalytic reduction of CO2. In this study, cotton-like blue C/TiO2NTs are successfully synthesized through the in situ growth of TiO2nanotubes on the MIL-125(Ti)-derived C/TiO2precursor. The experimental results revealed that the CO production rate of the cotton-like blue C/TiO2NTs was 1.84 times that of C/TiO2and 3.78 times that of TiO2nanotubes. These results clearly demonstrate that the cotton-like blue C/TiO2NTs exhibit a broad spectral response, a large specific surface area, and an abundance of oxygen vacancies. This research provides new insights into the design of titanium dioxide-based photocatalytic materials and opens up a promising avenue for enhancing the performance of titanium dioxide in the photocatalytic reduction of carbon dioxide.
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