BIOSENSORS & BIOELECTRONICS
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2022
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IF 12.545
Zhichao Yu,Hexiang Gong,Meijin Li,Dianping Tang
Multi-signal output biosensor technologies based on optical visualization and electrochemical or other sophisticated signal transduction are flourishing. However, sensors with multiple signal outputs still exhibit some limitations, such as the additional requirement for multiple regression equation construction and control of results. Herein, we developed a sensitive cascade of colorimetric-photothermal biosensor models for prognostic management of patients with myocardial infarction with the assistance of an artificial neural network (ANN) normalization process. A cascade enzymatic reaction device based on hollow prussian blue nanoparticles (h-PB NPs), and a portable smartphone-adapted signal visualization platform were integrated into the all-in-one 3D printed assay device. Specifically, liposomes encapsulated with h-PB were confined to the test cell using a classical immunoassay . Based on the peroxidase-like activity of h-PB, the h-PB obtained by the immunization process was further transferred to the TMB-H 2 O 2 system and used as a cascade of signal amplification for sensitive determination of cTnI protein. The target concentration was converted into a measurable temperature signal readout under 808 nm NIR laser excitation , and the absorbance of the TMB (ox-TMB) system at 650 nm was recorded simultaneously as a reference during this process. Interestingly, a parallel 3-layer, 64-neuron ANN learning model was built for bimodal signal processing and regression. Under optimal conditions, the bimodal machine learning-assisted co-immunoassay exhibited an ultra-wide dynamic range of 0.02–20 ng mL −1 and a detection limit of 10.8 pg mL −1 . This work creatively presents a theoretical study of machine learning-assisted multimodal biosensors, providing new insights for the development of ultrasensitive non-enzymatic biosensors.
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JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-19
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IF 9.429
Jia Tao,Li Dan,Du Jiarui,Fang Xikui,Gerasimov Valeriy,Ågren Hans,Chen Guanying
Nanozymes are promising for precise cancer treatment, but are typically limited in terms of the low catalytic efficiency and the complexity in tumor microenvironment (TME). Herein, we describe a bimodal type of AgPd plasmonic blackbody (AgPd PB) nanozyme of compact sizes (< 30 nm), which presents not only boosted enzyme efficacy but also efficient photothermal therapy (PTT) for synergized therapy through tissue-penetrating light in the second biological window (1000–1700 nm). The synthesized hyperbranched AgPd PB nanozymes possess intense and broadband localized surface plasmonic resonance absorption of 400–1300 nm, entailing prominent photothermal efficiency (η = 45.1% at 1064 nm) for PTT. Importantly, PTT was found to significantly boost the nanozyme efficacy of both catalase (CAT) and peroxidase (POD) processes, which correspondingly decompose H2O2 to into O2 to relieve tumor hypoxia, and activate H2O2 to generate oxidative •OH radical. While the generated •OH was found to be able to minimize heat shock proteins (HSPs), which plays a vital role to counterbalance PTT effect both in vitro and in vivo. As compared to control ground without treatment, the synergized nanozyme and PTT activities resulted in about 7-fold reduction of tumor volume, thus elevating the survival rate from 0 to 80% at 30 days posttreatment. Besides the synergistic therapy, the AgPd PB nanozyme were shown to own fluorescence, computed tomography (CT), and photoacoustic (PA) imaging abilities, thus having implications for uses in imaging-guided precise cancer therapy. This study provides a paradigm of TME responsive theranostics under NIR-II light irradiation.Graphical Abstract
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 17
·9698
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IF 6.208
Dong Wan,Yujun Liu,Xinhao Guo,Jianxin Zhang,Jie Pan
To endow the polymeric prodrug with smart properties through a safe and simple method, matrix metalloproteinase (MMPs) responsive peptide GPLGVRGDG was introduced into the block copolymer to prepare TPGS3350-GPLGVRGDG-DOX&DOX micelles, where TPGS3350is D-α-tocopheryl polyethylene glycol 3350 succinate. During the doxorubicin delivery, the cleavage of the peptide chain triggers de-PEGylation, and the remaining VRGDG sequence was retained on the surface of the micelles, which can act as a ligand to facilitate cell uptake. Moreover, the cytotoxicity of TPGS3350-GPLGVRGDG-DOX&DOX micelles against 4T1 cells was significantly improved, compared with TPGS3350-GPLGVRG-DOX&DOX micelles and TPGS3350-DOX&DOX micelles. During in vivo studies, TPGS3350-GPLGVRGDG-DOX&DOX micelles exhibited good anticancer efficacy with long circulation in the body and more efficient accumulation at the tumor site. Therefore, TPGS3350-GPLGVRGDG-DOX&DOX micelles have improved antitumor activity and reduced toxic side effects. This work opens new potential for exploring the strategy of drug delivery in clinical applications.Keywords:drug delivery;peptide;micelles
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Bioactive Materials
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2022
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IF 16.874
Sunfan Li,Fangzhou Li,Dong Wan,Zuqin Chen,Jie Pan,Xing-Jie Liang
Chemotherapy remains the mainstay of cancer treatment, benefiting millions of patients each year, but the side effects of chemotherapy drugs severely limit their clinical use. Doxorubicin (DOX) can cause various side effects such as heart damage and treatment-related tumors. The effective use of active and passive targeting will improve the clinical application of DOX. Here, TPGS 3350 and bioactive peptides were utilized to construct a micelle-based stage-by-stage impelled efficient system (missiles) for DOX delivery (DOX missiles). By taking advantage of the EPR effect, DOX missiles are efficiently enriched at the tumor site. After being cleaved by matrix metalloproteinase2 (MMP2), the peptide (VRGD) targets tumor cells to facilitate uptake of the missiles by the tumor cells via receptor-mediated endocytosis. The intracellular activated caspase-3-catalyzed explosion of DOX missiles further enables efficient tumor killing. This study provides an efficient approach for DOX delivery and toxicity reduction.
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Shenglan Hu,Dongmiao Qin,Shuo Meng,Yusheng Wu,Zhi Luo,Biyang Deng
Herein, an ultrasensitive electrochemiluminescence (ECL) sensor was established for the determination of dopamine using sulfur quantum dots (SQDs) as the luminophore. The detection mechanism highlights how dopamine can be oxidized into dopamine quinone (DQ) and quenches the ECL signal of SQDs. Under the optimized experimental conditions, the ECL signal of SQDs was used for the detection of dopamine in the linear range of 1 × 10 −10 to 1 × 10 −3 M with a detection limit of 2.5 × 10 −11 M (S/N = 3). The proposed method has excellent specificity, a low detection limit, and high sensitivity when applied in the analysis of real samples and is applicable in the development of novel ECL luminophores as well as the improvement of human health.
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DESALINATION
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2022
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IF 11.211
Zhongbao Ma,Long-Fei Ren,Diwen Ying,Jinping Jia,Jiahui Shao
Thin-film composite polyamide (TFC-PA) nanofiltration membrane is promising in heavy metal removal due to its excellent performance and high economic benefits. However, the common interfacial polymerization (IP) fabrication method requires abundant toxic and harmful solvents, causing harm to the environment and humans. Therefore, novel green, low-carbon and sustainable fabrication methods are urgently needed. Here, we reported a sustainable electrospray polymerization (SEP) technique for TFC-PA membrane preparation via electrospray featuring no toxic solvent, near-complete monomer conversion , and zero waste liquid discharge. In this process, 2-methyl-tetrahydrofuran and ethanol were used as green solvents, and its greenness was assessed by green metrics and toxicity bioassays. Physical features and physicochemical properties of SEP membranes were characterized by SEM, TEM, AFM and XPS. Membranes with different surface charges and pore sizes could be tailored by varying the monomer concentration . The optimal membrane exhibited excellent removal efficiency of toxic heavy metal ions , in which the rejection rates of Cu 2+ and Ni 2+ were higher than 97.0 %. In addition, the permeability and salt rejection of SEP membranes were better than those of IP membranes. Our sustainable electrospray polymerization method provides a versatile green platform for fabricating TFC membranes and may facilitate the development of sustainable nanofiltration membranes.
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ACS Applied Materials & Interfaces
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2022
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IF 10.383
Wei Wang,Jinyang Zheng,Hao Zhou,Qiang Liu,Li Jia,Xiuming Zhang,Dongtao Ge,Wei Shi,Yanan Sun
Overproduction of reactive oxygen species (ROS) and cumulative oxidative stress induce the degeneration of neuromelanin-containing dopaminergic neurons in the substantia nigra pars compacta (SNpc) of PD patients. Due to its redox property, melanin-like polydopamine (PDA) has been studied for its ability to remove ROS with a series of antioxidant enzyme mimetic activities including superoxide dismutase (SOD) and catalase (CAT). Glutathione peroxidase (GPx) is important for maintaining ROS metabolic homeostasis, but only a few GPx-like nanozymes have been studied for in vivo therapy. As we know, selenocysteine is essential for the antioxidant activity of GPx. Hence, we co-synthesized PDA with selenocystine (SeCys) to prepare a nanocomposite (PDASeCys) with GPx-like activity. The results showed that the PDASeCys nanocomposite has the same CAT and SOD enzymatic activities as PDA but better free radical scavenging efficiency and additional GPx enzymatic activity than PDA. In the 1-methyl-4-phenyl-pyridine ion (MPP+)-induced PD cell model, PDASeCys could increase intracellular GPx levels effectively and protect SH-SY5Y neuronal cells from oxidative stress caused by MPP+. In vivo, the PDASeCys nanocomposite effectively inhibited 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridinium (MPTP)-induced Parkinson-related symptoms of mice when it was injected into the substantia nigra (SN). This polydopamine-based nanocomposite containing selenocystine with a variety of enzymatic activities including GPx-like activity synthesized by a one-pot method provides convenience and safety in the neuromelanin-like nanozyme-based therapeutic strategy for oxidative stress-induced PD.
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BIOMATERIALS
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2022
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IF 15.304
Haiyan Pan,Quan Zou,Tingting Wang,Dong Li,Shao-Kai Sun
Diverse oxygen generation strategies have been developed to overcome hypoxia in tumors for enhancing the therapeutic efficacy, but inevitably suffering from tedious synthesis process of oxygen generators in vitro before in vivo administration. Herein, we show direct injection of commercially and clinically used KMnO 4 into solid tumors enables in situ formation of MnO 2 as an oxygen depot for cascade oxidation damage and enhanced photodynamic therapy. KMnO 4 can damage tumor tissues by oxidation and generate MnO 2 , and subsequent intravenous injection of Ce6 allows MnO 2 -triggered hypoxia-modulated photodynamic therapy of tumors. Excellent cascade tumor suppression effect is realized both in vitro and in vivo based on the KMnO 4 –Ce6 system without the need of synthesis. The proposed strategy lays down a novel way with unprecedented superiors of no need of synthesis process and ultra-facile administration procedure for tumor hypoxia-modulated cascade therapy.
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DYES AND PIGMENTS
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2022
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IF 5.122
Selvaraj Muthusamy,Siqi Yin,Kanagaraj Rajalakshmi,Suci Meng,Dongwei Zhu,Meng Xie,Jimin Xie,Rathna Silviya Lodi,Yuanguo Xu
Hydrazine is a highly toxic pollutant to the environment and living organisms. Therefore, it is an urgent need to detect hydrazine selectively, sensitively, and successfully in the real samples and biosystems . Thus, we designed a donor-π-acceptor fluorophore quinoline ( Q ) appended 4-formylphenyl 2,4-dinitrobenzoate ( P-DNP ) to form a Schiff base QP-DNP for tracking hydrazine in real samples and biosystems. The probe observes an intramolecular charge transfer and aggregation induced emission sensing mechanisms on cleavage of ester bond upon nucleophilic substitution reaction with hydrazine and exhibited a “turn-on” fluorescence at green channel with the formation of quinoline-phenol. The proposed study was investigated through photophysical properties, 1 H NMR titration, HR-MS experiments, and density-functional theory calculations. The LOD of QP-DNP for hydrazine was calculated to be 55 nM and exhibited a high selectivity among possible competitive analytes. Additionally, the probe showed a remarkable emission colour changes on fabrication of paper sensor strips that can be discriminated in monitoring hydrazine vapor using smartphone and achieved an AND gate operation. Furthermore, QP-DNP was successfully applied to fluorescence imaging of hydrazine in live HeLa cells , monitoring of in situ hydrazine release as a metabolite of isoniazid drug administrated orally and determining hydrazine contamination in real samples.
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DALTON TRANSACTIONS
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2022
·Vol. 51
·期 30
·11325-11334
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IF 4.569
Yale Hong,Qinfeng Tao,Yuan-Yuan Liu,Zhuo Wang,Haifang Wang,Lining Sun
Chemodynamic therapy (CDT) can convert endogenous hydrogen peroxide (H2O2) to highly reactive hydroxyl radical (˙OH) through Fenton or Fenton-like reaction to kill tumor cells, which is a promising anticancer strategy. However, the limited H2O2 and overexpressed glutathione (GSH) in tumor cells make CDT ineffective. Here, an efficient nanocomposite, UCN@CuO2–GOx (UCCuG), was synthesized, realizing both starvation therapy and H2O2 self-supplying CDT in vitro. In this case, the glucose oxidase (GOx) of the nanocomposite could consume glucose for starvation therapy after the UCCuG nanocomposite entered tumor cells. In addition, the acidic environment of the lysosome triggered the release of Cu2+ and H2O2 by the decomposition of UCCu; then, Cu2+ was reduced to Cu+ by GSH in tumor cells; and finally, Cu+ catalyzed the released H2O2 to generate ˙OH for CDT. The in vitro experiments demonstrated starvation-enhanced CDT with remarkable results. Meanwhile, under 980 nm laser irradiation, the upconversion luminescence signal of UCN in the UCCuG nanocomposite was reduced due to the CuO2–GOx coating, while it gradually recovered after the UCCuG nanocomposite reacted with glucose and GSH under the tumor microenvironment (TME). Such a luminescent intensity recovery process is expected to monitor the TME-activated therapeutic effect in real time. This strategy may solve the problem of insufficient CDT efficacy caused by limited endogenous H2O2 and overexpressed GSH in tumor cells. This multifunctional nanocomposite demonstrates the promising application of starvation-enhanced CDT in tumor treatment.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Lixia Han,Ting Liu,Di Cui,Jiaqi Yi,Wei Jiang,Xiaoshuang Li,Na Niu,Ligang Chen
Captopril (CP) is a widely used antihypertensive drug. In this study, a smartphone-assisted sensing platform on the basis of ratiometric fluorescent test strips was developed, which can accomplish visualization for the quantitative detection of captopril. Ratiometric fluorescent probe was constructed from carbon dots (CDs) and gold nanoclusters (Au NCs). After adding Cu 2+ to fluorescent probe, Cu 2+ can complex the amino and carboxyl groups on the surface of Au NCs and aggregate Au NCs, which will quench the fluorescence of Au NCs. Compared with amino and carboxyl groups, -SH in CP has a higher affinity for Cu 2+ and can capture Cu 2+ to restore Au NCs fluorescence. In this process, CDs remained essentially unchanged as background fluorescence. As CP concentration increased, the fluorescence color showed a distinct change from blue to purple to orange. Based on this principle, a sensing platform combining smartphone and fluorescent test strips was constructed to visualize the quantitative detection of CP by RGB values. Under optimal conditions, the wide linear range of CP detection for both fluorescence spectrometer and smartphone paper-based sensing platform was 0.25–50 μM. The limits of detection were as low as 76 nM and 101.3 nM, respectively. Furthermore, it was implemented successfully for the detection of CP in urine. The satisfactory recoveries were 96.0–103.3% and 92.0–108.0% for fluorescence spectrometer and smartphone platform, respectively. This smartphone-assisted platform provided a new approach for visual detection of CP, which showed its great potential in bioanalytical assays.
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BIOSENSORS & BIOELECTRONICS
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2022
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IF 12.545
Qilin Zhao,Yu Wang,Mengqian Zhang,Donghui Wu,Jian Sun,Xiurong Yang
A facile and sensitive method for sensing α-glucosidase (α-glu) and screening its inhibitors based on fluorescence monitoring of water-solute silicon-containing nanoparticles (Si CNPs) was proposed and demonstrated. Such fluorescent nanoparticles can be easily produced by mixing (3-aminopropyl) trimethoxysilane (APTMS) and ascorbate sodium (AS) (both without fluorescence signals) at room temperature and pressure. If the ascorbate sodium was replaced by L-ascorbic acid-2-O-α-D-glucopyranosyl (AA2G), which can be hydrolyzed into the former by α-glu, the fluorescence “turn-on” biosensor for α-glu activity can be established. The sensing platform showegd a linear relationship from 10 to 140 U/L and a low detection limit of 0.42 U/L, which is superior to most approaches that have been reported. However, the hydrolysis procedure and subsequent fluorogenic reaction could be blocked in the presence of α-glu inhibitors (AGIs), giving the possibilities of screening various inhibitors from different compounds. Furthermore, detection in human serum and applications in AGIs screening by using this method were also constructed, and showed satisfying results as well. It is proved that this developed biosensor can provide an alternative strategy for potential clinic diagnosis and medicine development.
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