Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Yansheng Liu,Zhenle Qin,Xiaobo Jia,Jin Zhou,Hongli Li,Xiaohong Wang,Guofu Wang
Plastics as very important materials have been extensively utilized, and they make people’s lives comparative and economic. However, recent studies find that the micro/nano-plastic particles degenerating from plastic materials especially for food containers may lead to some unknown diseases when they enter into the human lung or stomach. The methods of detecting micro/nano-plastic particles are urgently required, and conventional methods necessitate intricate pre-processing steps and specialized equipment. Hence, this paper introduces a highly sensitive and direct detection method based on Surface-Enhanced Raman Spectroscopy (SERS) in quantitatively and qualitatively analyzing polystyrene (PS) nanoplastics that are drop cast on substrate. The mechanism of SERS performance of the proposed Ag core embedded Au film (Ag@AuFilm) SERS substrate is studied by using the finite-different time-domain (FDTD) method. By optimizing the gap distance between the Ag core and the Au film, PS nanoplastics with three diameters were used to validate the effectiveness of the SERS substrate. The limits of detection (LOD) for PS nanoparticles with diameters of 50 nm, 70 nm, and 310 nm in DI water and real-river water were the same and the corresponding LODs were 50, 50, and 25 μg/mL, respectively. Thus, this study proposes an easily fabricated SERS substrate and provides a rapid, quantitative, and qualitative method for detecting PS nanoplastics. This new way of addressing the pervasive issue of nanoplastics pollution provided an accurate, efficient, and rapid detection of the nanoplastics.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Tianxing Fang,Qingyi Wei,Erwen Wu,Hongbin Pu
Malachite green (MG)-contaminated aquatic products pose a serious threat to animal and human health. Hence, a novel recyclable surface-enhanced Raman scattering (SERS) substrate based on AuNPs/TiO 2 /Ti 3 C 2 heterostructures was developed for the detection and degradation of MG in aquatic products. Specifically, AuNPs/TiO 2 /Ti 3 C 2 heterostructures were synthesized by in situ oxidation and electrostatic adsorption based on Ti 3 C 2 nanosheets. The excellent photocatalytic and SERS performance of the AuNPs/TiO 2 /Ti 3 C 2 was demonstrated by Density functional theory (DFT) calculations and experimental results, which was attributed to the enhancement of charge transfer (CT) after the formation of heterostructures. The results demonstrate that AuNPs/TiO 2 /Ti 3 C 2 is highly sensitive and recyclable. The detection limit of the sensor for MG is 8.91 × 10 −5 mg/L. The sensor can be recycled for five times under the condition of light, and shows satisfactory self-cleaning performance in the food matrix, providing a possible alternative solution for the recyclable detection of MG.
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Food Analytical Methods
·
2024
·
IF 2.6
Ma Xiaoyuan,Wei Jinxiang,Jiang Caiyun,Wang Zhouping
Herein, an aptasensor with stable surface-enhanced Raman scattering (SERS) and fluorescence dual-signal output had been developed for the detection of aflatoxin B 1 (AFB 1 ). The quantum dots were encapsulated within silica to ensure the stability of fluorescence signal. The gold nanoparticles (AuNPs) were modified with SERS signal molecule DTNB and the complementary sequence (cDNA) of AFB 1 aptamer and then connected on the surface of silica to obtain the signal probes. In the meantime, Fe 3 O 4 was coated with silica which was modified with aptamer to gain capture probes. The two probes could bind specifically based on the base complementary pairing principle. Then, the mixture was separated and gathered by an external magnet. At this time, the maximum SERS signal could be detected in the precipitate, and there was no fluorescence signal in the supernatant. Upon introduction of AFB 1 into the system, the signal probes would be released, resulting in fluorescence recovery in the supernatant while SERS signal weakened in the precipitate. The developed method showed a linear relationship of AFB 1 for the SERS signal in the range of 10 −4 –10 3 ng/mL, with a detection limit of 0.087 pg/mL. The linear range for the fluorescence signal was from 10 −4 to 10 2 ng/mL, with a detection limit of 0.100 pg/mL. The proposed technique was highly sensitive and accurate, which showed promising application prospects in food safety detection.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wei Zhang,Qiao Tang,Mengmeng Wang,Pei Liang,Xuejiao Zhang,Kun Pang,Qiang Chen,Xiaoping Yu
In this work, an aptamer sensor (aptasensor) for SERS detection of Vibrio parahaemolyticus was prepared by utilizing flower-shaped silver nanoparticles coated with graphene oxide (Ag@GO) and core–shell Au@Ag nanoparticles embedded with the Raman reporter 4-mercaptobenzoic acid (4MBA). Graphene oxide (GO) was synthesized using an improved Hummers method and wrapped around thiol-functionalized Ag nanoflowers to form the Ag@GO composite. The SERS aptamer sensor with the strongest Raman signal of 4MBA was developed by combining Ag@GO with Au@4MBA@Ag NPs modified by aptamer through hydrogen bonding and π-π stacking interactions. The aptasensor exhibited good repeatability and stability, and demonstrated high specificity. And it showed a good linear relationship for Vibrio parahaemolyticus detection within the concentration range of 10 2 cfu/mL–10 7 cfu/mL. Introducing GO as an internal standard improved the linear regression coefficient (R 2 ) of detection from 0.9885 to 0.9956. Additionally, spiked real samples of fresh shrimp were tested, yielding recovery rates between 94.1 % and 104.7 %, which demonstrate the feasibility and practicality of this method.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jing Wu,Ying Zhang,Jiuchuan Wang,Zhuangzhuang Ling,Xuanhua Yan,Xiaoming Lyu,Jinghuai Fang,Mingfei Cheng,Min Zhao,Tao Ban,Yu Liu,Yang Li
In the realm of disease diagnostics, particularly for conditions such as proteinuria and hemoglobinuria, the quest for a method that combines accurate, label-free detection of protein compositions and their conformational changes remains a formidable challenge. In this study, we introduce an innovative Ag/Au plasmonic hybrid coupling nanoarray (Ag/Au PHCN) architecture marked by sub-10 nm interparticle gaps. These nanoarrays, leveraging plasmonic hybrid coupling and synergistic enhancement mechanisms, create a plethora of uniform surface-enhanced Raman spectroscopy (SERS) hotspots. The Ag/Au PHCN substrates demonstrated unparalleled sensitivity in the unmarked detection of hemoglobin (HGB), bovine serum albumin (BSA), and cytochrome C (Cyt.C) in bodily fluids, incorporating the advantages of high sensitivity, high reproducibility, durability, recyclability, and biocompatibility. Notably, the detection limits for BSA and HGB are unprecedented at 0.5 and 5 ng/mL, respectively. This achievement sets a new benchmark for label-free protein detection using two-dimensional nanostructures. Crucially, the Ag/Au PHCNs possess the novel capability to discern protein conformational changes post denaturation, underscoring their potential in probing protein functionalities. Most importantly, these nanoarrays can differentiate between normal and proteinuria-affected urine samples and monitor protein content variations over time, heralding a new era in clinical diagnostics with particular relevance to proteinuria and hemoglobinuria detection.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Lan Zhang,Tingting Zhang,Shiying Wu,Lujie Li,Wei Li,Lingling Wang,Weihua Li,Xianhuai Huang,Jiansheng Li,Rui Lu
Although surface-enhanced Raman scattering (SERS) is a powerful spectral analysis technique, its practical application is restricted due to the poor chemical stability of some noble metal nanoparticles. In this work, Ag@ZIF-8 nanocomposites with different ZIF-8 core-shell thicknesses were prepared by coating AgNP surfaces with polyvinylpyrrolidone and adjusting the dosage of zinc nitrate and 2-methylimidazole. Several characterizations have confirmed that the Ag@ZIF-8 nanocomposites was successfully prepared. Based on transmission electron microscopy images and SERS spectral intensity, it was determined that Ag@ZIF-8 nanocomposites with a ZIF-8 shell thickness of 30 nm exhibited optimal SERS performance. As a result of evaluating the uniformity and stability of the composite with 4-ATP as the probe molecule, it showed a relative standard deviation of 6.91 % and a decrease in SERS intensity of 4.5 % after 28 days. Based on the results obtained with Ag@ZIF-8 nanocomposites as the SERS substrate, the detection limits of 4-mercaptopyridine and thiram were as low as 5.87×10 −9 M and 6.61×10 −8 M, respectively. Furthermore, the finite-difference time-domain simulation reveals an enhancement factor of 2.6×10 6 , which is in good agreement with the experimental value of 1.8×10 6 , confirming its effectiveness as a SERS substrate.
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Nanoscale
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2012
·Vol. 4
·期 16
·5210-5216
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IF 5.914
Qiao An,Peng Zhang,Jun-Mei Li,Wan-Fu Ma,Jia Guo,Jun Hu,Chang-Chun Wang
Highly active surface-enhanced Raman scattering (SERS) substrates of Ag nanoparticle (Ag-NP) modified Fe3O4@carbon core–shell microspheres were synthesized and characterized. The carbon coated Fe3O4 microspheres were prepared via a one-pot solvothermal method and were served as the magnetic supporting substrates. The Ag-NPs were deposited by in situ reduction of AgNO3 with butylamine and the thickness of the Ag-NP layer was variable by controlling the AgNO3 concentrations. The structure and integrity of the Fe3O4@C@Ag composite microspheres were confirmed by TEM, XRD, VSM and UV-visible spectroscopy. In particular, the Ag-NP coated Fe3O4@carbon core–shell microspheres were shown to be highly active for SERS detections of pentachlorophenol (PCP), diethylhexyl phthalate (DEHP) and trinitrotoluene (TNT). These analytes are representatives of environmentally persistent organic pollutants with typically low SERS activities. The results suggested that the interactions between the carbon on the microsphere substrates and the aromatic cores of the target molecules contributed to the facile pre-concentration of the analytes near the Ag-NP surfaces.
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Analytical Methods
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2024
·Vol. 16
·期 14
·2085-2092
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IF 2.7
Yue Liu,Guanwen Su,Wei Wang,Hongyuan Wei,Leping Dang
This study proposes a microfluidic platform for rapid enrichment and ultrasensitive SERS detection of bacteria. The platform comprises ZnO nanoflower arrays decorated with silver nanoparticles to enhance the SERS sensitivity. The ZnO nanoflower array substrate with a 3D reticular columnar structure is prepared using the hydrothermal method. SEM analysis depicts the 3.05 μm gap distribution of the substrate array to intercept the most bacteria in the particle sizes range of 0.5 to 3 μm. Then, silver nanoparticles are deposited on the ZnO nano-array surface by liquid evaporation self-assembly. TEM and SEM analysis indicate nanosize of Ag particles, evenly distributed on the substrate, enhancing the SERS efficiency and improving sensing reproducibility. The probe molecules (R6G) are tested to demonstrate the high SERS activity of the proposed microfluidic sensor. Then, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis are selected, demonstrating the sensor's excellent bacterial capture and sensitive recognition capabilities, with a detection limit as low as 102 CFU mL−1. Additionally, the antibacterial properties of ZnO/Ag heterojunction nanostructures are studied, suggesting their ability to inactivate bacteria. Compared with the traditional Au-enhanced chip, the sensor preparation is easy, safe, reliable, and low-cost. Moreover, the ZnO nano-array exhibits a large specific surface area, high interception ability, stronger and uniform SERS performance, and effective and reliable detection of trace pathogens. This work provides potential future ZnO/Ag microfluidic SERS sensor applications for rapid, unlabeled, and trace pathogens detection in clinical and environmental applications, potentially achieving breakthroughs in early detection, prevention, and treatment.
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NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Zhiyuan Sun,Gege Zhao,Ming Guan,Huixian Li,Fang Mi,Xiaobo Ma,Yajun Zhao,Han Song
In this work, boric acid affinity magnetic materials and Au nanorods capped by Ag (AuNRs@Ag) core-shell structure were used to construct the sandwich structure to obtain surface-enhanced Raman scattering (SERS) sensor for detecting glucose. The AuNRs@Ag modified with 4-mercaptophenylboronic acid (4-MPBA) as SERS substrate, illustrating the efficient electromagnetic enhancement. Moreover, the boric acid affinity magnetic materials (FMNPs) were further functionalized with knife bean lectin as “capture probe”, which illustrated the high adsorption capacity to glucose. The maximum adsorption amount can reach 33.3 mg/g. The conditions for preparing SERS platform as well as glucose detection were optimized. Under the optimal experimental conditions, the detection linear range for glucose is 1.0×10-11 ~ 1.0×10-3 mol/L with a limit of detection (LOD) 1.8×10-12 mol/L (S/N = 3). In addition, the developed SERS sensor was further applied for determination of glucose in human urine with recoveries in the range from 97.5% to 101.5%. As expected, the prepared sandwich SERS sensor was successfully employed to recognize and detect glucose with high sensitivity.
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Chemosensors
·
2024
·Vol. 12
·期 9
·187
·
IF 3.7
Yuyu Peng,Chunyan Wang,Gen Li,Jianguo Cui,Yina Jiang,Xiwang Li,Zhengjie Wang,Xiaofeng Zhou
In this study, a MOF-199/Ag@Au SERS sensing structure was successfully synthesized by combining metal–organic frameworks (MOFs) with surface-enhanced Raman scattering (SERS) technology for the efficient detection of dopamine (DA), a biomarker for neurological diseases, in serum. Using electrochemical methods, a copper-based MOF (MOF-199) was synthesized in situ on copper substrates and further deposited with silver nanoparticles (AgNPs). Subsequently, gold nanoshells were encapsulated around these silver cores by in situ chemical deposition. This preparation process is simple, controllable, and inexpensive. Furthermore, a novel Azo reaction-based DA SERS method was proposed to detect 1 pM DA, which represents an improvement in sensitivity by two orders of magnitude compared to previous unlabeled SERS detection methods and by four orders of magnitude compared to another SERS approach proposed in this work. There was an excellent linear relationship (R2= 0.976) between the SERS signal at 1140 cm−1and the DA concentration (0.001 M~1 pM). The results indicate that the MOF-199/Ag@Au sensor structure can successfully achieve both the qualitative and quantitative detection of DA in serum, thus providing a robust technical basis for the application of SERS technology in the field of clinical neurological disease screening.
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ACS Sensors
·
2025
·
IF 9.1
Chunyan Zhu,Xiaodong Lin,Ruyun Jin,Caini Long,Chunnan Zhu,Dongyun Zheng,Xiaofei Yang,Pan Zhang,Chao Liu
The clinical translation of exosome-based diagnostics and therapies is hindered by significant molecular heterogeneity. Current exosome phenotyping methods suffer from insufficient sensitivity, limited multiplexing capability, and poor scalability. To address these challenges, we developed a digital wafer-level micropillar array (WLM-Exo) platform. The chip features a high-surface-area micropillar array functionalized with a DSPE-PEG2000 lipid layer via Au–S bonding, enabling lipid-mediated membrane fusion for universal exosome enrichment and a uniform spatial distribution. Following capture, aptamer-based SERS nanotags bind specifically to target proteins on exosomes, generating distinct Raman signals upon laser excitation. Exosomal proteins are digitally quantified by measuring the activated area in the Raman images, exhibiting a log–log linear correlation with exosome concentration. The WLM-Exo platform demonstrates ultrasensitive detection (LOD: 2.11 particles/μL for CD63, 1.17 particles/μL for EpCAM, and 18.3 particles/μL for dual-biomarkers) and simultaneous tetra-color exosomal phenotyping capability. By enabling precise, scalable, and digital protein profiling, this technology offers significant potential for exosomes subtyping in personalized cancer diagnostics and therapeutics monitoring.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingting Hu,Haimei Zeng,Hao Peng,Guanchao Yin,Jihong Zhang,Jun Xie,Yang Jiang
With the increasing discharge of pollutants into air, water, and soil, the rapid and sensitive detection and treatment of chemical pollutants, including toxic organic dyes and heavy metal ions, have become critical for minimizing potential threats to the ecological cycle and human health. In the current research, an in-situ degradation approach for toxic substances is proposed, combined with surface-enhanced Raman scattering (SERS). Fiber probes based on a silver nanoparticles/ silver phosphate tetrapod (Ag/ Ag 3 PO 4 ) composite were fabricated using a sequence of ion exchange, chemical reduction, and subsequent coating processes. The fiber probes were deployed for ultra-sensitive SERS detection and in situ photocatalytic degradation of dyes (specifically crystal violet (CV)) and heavy metal ions (particularly Hg 2+ ). The probes were functionalized with 4-mercaptopyridine (4-MPY) to facilitate the detection of Hg 2+ . Notably, the probes demonstrated detection limits as low as 10 −14 M for crystal violet (CV) and 4-aminothiophenol (PATP), and 10 −12 M of Hg 2+ . Under light exposure, the substrate degraded 93.2 % of CV and 98.0 % of PATP within 60 min, and 82.3 % of Hg 2+ within 30 min, as monitored in situs. Finite element method analysis suggested that the high electromagnetic field intensity generated by Ag/ Ag 3 PO 4 resulted from the close packing of Ag nanoparticles. Furthermore, the SERS fiber probes exhibited outstanding stability, selectivity, and reusability. This research suggest that the recyclable Ag/ Ag 3 PO 4 fiber probes hold significant potential for the detection and in situ monitoring of the degradation of trace environmental contaminants.
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危险品化学品经营许可证（带存储）