JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Jun Yang,Jieqiong Lin,Weidong Zhang,Mingyue Shen,Yuanxing Wang,Jianhua Xie
BACKGROUND Resveratrol (Res) is promising food functional factor with favorable antioxidant and anti-inflammatory properties, although its poor water solubility and low bioavailability limit extensive application. Therefore, in combination with another promising polysaccharide ( Mesona chinensis polysaccharides, MCP), Res-loaded food nanocarriers (ResNPs) were developed to increase its water solubility, bioactivity and targeting properties. ResNPs were then applied to alleviate dextran sulfate sodium (DSS)-induced ulcerative colitis. RESULTS Resveratrol can be well encapsulated in MCP-based nanoparticles in an amorphous state, improving its water solubility. ResNPs showed pH-response controlled release behavior in the gastrointestinal tract and increased the enrichment of Res in the colon. In vivo experiments of ResNPs against DSS-induced ulcerative colitis (UC) revealed that ResNPs significantly improved UC symptoms, modulated intestinal inflammation and down-regulated oxidative stress levels compared to free Res. ResNPs also play an positive role with respect to inhibiting the mitogen-activated protein kinase pathway and promoting the expression of tight junction proteins. In addition, ResNPs improved the species composition and relative abundance of intestinal flora in UC mice, which effectively regulated the balance of intestinal flora and promoted the production of short-chain fatty acids. CONCLUSION These results suggest that MCP-based nanoparticles can effectively improve the solubility of resveratrol and enhance its in vivo bioactivity. Moreover, the present study also provides a new strategy for the prevention and treatment of UC with food polyphenol. © 2024 Society of Chemical Industry.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2024
·
IF 3.9
Linrong Shi,Mingzhi Zhu,Ruimin Long,Shibin Wang,Pei Wang,Yuangang Liu
In recent years, there has been growing interest in size-transformable nanoplatforms that exhibit active responses to acidic microenvironments, presenting promising prospects in the field of nanomedicine for tumor therapy. However, the design and fabrication of such size-adjustable nanotherapeutics pose significant challenges compared to size-fixed nanocomposites, primarily due to their distinct pH-responsive requirements. In this study, we developed pH-activated-aggregating nanosystems to integrate chemotherapy and photothermal therapy by creating size-transformable nanoparticles based on Prussian blue nanoparticles (PB NPs) anchored with acid-responsive polyoxometalates (POMs) quantum dots via electrostatic interactions (PPP NPs). Subsequently, we utilized doxorubicin (DOX) as a representative drug to formulate PPPD NPs. Notably, PPPD NPs exhibited a significant response to acidic conditions, resulting in changes in surface charge and rapid aggregation of PPP NPs. Furthermore, the aggregated PPP NPs demonstrated excellent photothermal properties under near-infrared laser irradiation. Importantly, PPPD NPs prolonged their retention time in tumor cells via a size-transformation approach. In vitro cellular assays revealed that the anticancer efficacy of PPPD NPs was significantly enhanced. The IC50 values for the PPPD NPs groupand the PPPD NPs + NIR group were 50.11 μg/mL and 30.9 μg/mL. Overall, this study introduces a novel strategy for cancer therapy, by developing size-aggregating nano-drugs with stimuli-responsive properties, holding promise for improved therapeutic outcomes in future combination approaches involving photothermal therapy and chemotherapy.
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BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2025
·Vol. 115
·期 3
·1-7
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IF 2.2
Wang Yun,Shan Ning,Habibul Zumrat,Habibul Nuzahat
This study investigated the impact of pH on the combined toxicity of polystyrene microplastics (MPs) and ciprofloxacin (CIP) on ryegrass growth and CIP uptake. Two different sizes of PS-COOH (200 and 500 nm) were utilized. The findings indicated that neutral and alkaline pH conditions (pH 7.0 and 8.8) significantly reduced CIP accumulation in ryegrass roots, regardless of MP presence. Conversely, CIP content in shoots (including leaves) increased with rising pH, showing 24.7-fold and 29.5-fold enhancements under CIP + 200 nm MPs and CIP + 500 nm MPs treatments at pH 8.8 compared to pH 5.3. Additionally, pH significantly influenced growth inhibition, with more pronounced effects at pH 7.0 and 8.8 than at pH 5.3. Under neutral and alkaline conditions, shoot lengths in 500 nm MPs + CIP and 200 nm MPs + CIP treatments were reduced to 50.3%, 69.7% and 86.8%, 81.3% of those at pH 5.3, respectively. These findings highlight the critical role of pH in modulating CIP uptake and toxicity in ryegrass, particularly in MP co-contaminated environments. Moreover, the relatively high CIP removal efficiency suggests the potential of ryegrass for CIP remediation in MP-polluted systems.
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LANGMUIR
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2019
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IF 3.683
Yawen Li,Angelina Angelova,Fangzhou Hu,Vasil M. Garamus,Changjun Peng,Na Li,Jianwen Liu,Dan Liu,Aihua Zou
We report pH-responsive liquid crystalline lipid nanoparticles, which are dual-loaded by Brucea javanica oil (BJO) and doxorubicin hydrochloride (DOX) and display a pH-induced inverted hexagonal (pH = 7.4) to cubic (pH = 6.8) to emulsified microemulsion (pH = 5.3) phase transition with a therapeutic application in cancer inhibition. BJO is a traditional herbal medicine that strongly inhibits the proliferation and metastasis of various cancers. Doxorubicin is an antitumor drug, which prevents DNA replication and hampers protein synthesis through intercalation between the base pairs of the DNA helices. Its dose-dependent cardiotoxicity imposes the need for safe delivery carriers. Here, pH-induced changes in the structural and interfacial properties of designed multicomponent drug delivery (monoolein–oleic acid–BJO–DOX) systems are determined by synchrotron small-angle X-ray scattering and the Langmuir film balance technique. The nanocarrier assemblies display good physical stability in the studied pH range and adequate particle sizes and ζ-potentials. Their interaction with model lipid membrane interfaces is enhanced under acidic pH conditions, which mimic the microenvironment around tumor cells. In vitro cytotoxicity and apoptosis studies with BJO–DOX dual-loaded pH-switchable liquid crystalline nanoparticles are performed on the human breast cancer Michigan Cancer Foundation-7 (MCF-7) cell line and MCF-7 cells with doxorubicin resistance (MCF-7/DOX), respectively. The obtained pH-sensitive nanomedicines exhibit enhanced antitumor efficacy. The performed preliminary studies suggest a potential reversal of the resistance of the MCF-7/DOX cells to DOX. These results highlight the necessity for further understanding the link between the established pH-dependent drug release profiles of the nanocarriers and the role of their pH-switchable inverted hexagonal, bicontinuous cubic, and emulsified microemulsion inner organizations for therapeutic outcomes.
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Journal of Materials Chemistry B
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2022
·Vol. 10
·期 17
·3375-3385
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IF 7.571
Jie Chang,Liufang Mo,Jinfeng Song,Xiaochen Wang,Hanhan Liu,Chenchen Meng,Yijun Wu
In order to make the drug specifically aggregate at the tumor site, we had developed a targeted drug delivery system based on pH responsive mesoporous silica nanoparticles. Mesoporous silica nanoparticles (MSN-COOH) were prepared and doxorubicin (DOX) was loaded into the pores of MSN-COOH, and then polyethyleneimine (PEI) and anisamide (AA) were modified on the surface of mesoporous silica, named DOX@MSN-PEI-AA(DMPA). DMPA specifically entered tumor cells through AA-mediated receptor endocytosis; PEI dissociated from the surface of the MSN in the acidic environment of cellular lysosomes/endosomes due to protonation of PEI, resulting in steady release of the encapsulated DOX from the pores of MSN in the cytoplasm of the target cells. In vitro and in vivo anti-tumor experiments and hemolytic experiments indicated that DMPA can accurately target breast cancer cells and show excellent safety at the same time, showing great potential for tumor therapy.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2019
·Vol. 38
·期 6
·587-592
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IF 2.804
Gao Si-Jia,Li Zhuang,Sun Zhi-Cheng,Wen Jin-Yue,Li Fu-Rong,Du Xiao-Yang,Liu Yan,Tian Ye,Niu Zhong-Wei
As a typical plant virus which has biocompatibility and high transfection efficiency, tobacco mosaic virus (TMV) has shown broad application potential in drug or gene delivery field. Elucidating its intracellular trafficking is of great importance in investigation of its cytotoxicity, targeting site, and delivery efficiency, and is advantageous to designing new TMV-based drug delivery systems with different targets. By taking advantage of the regulated pH value of different organelles in a mammalian cell, we exploit a pH detection strategy to investigate the intracellular trafficking pathway of TMV. Here, we report a single-wavelength excited ratiometric fluorescent pH probe. This probe is constructed by simultaneously coupling pH-sensitive fluorescein isothiocyanate (FITC) and pH-insensitive rhodamine B isothiocyanate (RBIRC) onto the inner surface of TMV. The fluorescence intensity ratio of FITC to RBITC excited at 488 nm responds specifically towards pH value over other interferential agents. By taking use of this single-wavelength excited ratiometric pH probe and confocal laser scanning microscopy, it is shown that the endocytosed TMV is located in a pH decreasing microenvironment and eventually enters lysosomes. This work may provide important guidance on construction of TMV-based nano carriers.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2019
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IF 6.361
Yujuan Chen,Abdur Rauf Khan,Dexin Yu,Yujia Zhai,Jianbo Ji,Yikang Shi,Guangxi Zhai
Traditional cancer therapies carry a risk of serious side effects and toxicity. Developing an alternative treatment modality that is highly effective, has low toxicity and is noninvasive is urgently required. Here, we exploited molybdenum oxide (MoOx) nanosheets as a drug carrier and degradable photothermal agent to provide a chemo-photothermal combination cancer therapy. The MoOx nanosheets were synthesized by a one-pot hydrothermal method and then modified with pluronic F127 to improve physiological stability and biocompatibility. The F127-modified nanosheets (MoOX@F127) showed ultrahigh drug loading efficiency (DLE) of doxorubicin (DOX) (DLE%; 65%, W (load DOX) /[W (load DOX) + W MoOx@F127 ]), strong near-infrared (NIR) absorption and desirable pH-dependent degradability. After intravenous injection, MoOx@F127 nanosheets were degraded at physiological pH and were rapidly excreted from normal organs, while they were effectively accumulated and retained long-term in the more acidic tumor tissue. This simultaneously ensured effective tumor ablation after NIR irradiation and avoided long-term retention and toxicity in vivo . Compared to chemotherapy or photothermal therapy alone, in vitro and in vivo tumor ablation studies have shown a notably improved synergistic effect of the combination therapy. Our study presents a multifunctional nanosystem with a desirable degradability for chemo-photothermal combination cancer therapy that has great potential in biomedical applications.
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ANALYTICAL CHEMISTRY
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2021
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IF 6.986
Hui Dong,Le Zhao,Yanli Zhou,Xiuhua Wei,Cuicui Xu,Yintang Zhang,Maotian Xu
Brain pH has been proven to be a key factor in maintaining normal brain function. The relationship between local pH fluctuation and brain disease has not been extensively studied due to lack of the accurate in situ analysis technology. Herein, we have for the first time proposed a voltammetric pH sensor by measuring the ratio of current signals instead of the previously reported potential based on the Nernst equation. Single-walled carbon nanotubes (CNT) were first self-assembled on the electrode surface of a carbon-fiber nanotip electrode (CFNE). Then, poly-o-phenylenediamine (PoPD) molecules were deposited as pH-responsive molecules through in situ electrochemical polymerization. The compact CFNE/CNT/PoPD exhibited a good redox process with the on–off–on ratiometric electrochemical response to pH ranging from 4.5 to 8.2, providing self-correction for in situ pH detection. Thus, the proposed sensor enabled the accurate measurement of pH with excellent selectivity even in the presence of proteins or electroactive species. In addition, the sensor showed high repeatability, reproducibility, and reversibility in measuring pH and even demonstrated good stability when it was exposed to air for 5 months. Finally, we successfully detected the fluctuation of pH in rat brains with cerebral ischemia and rat whole blood. Overall, this research not only provides a good tool for the detection of rat brain pH but also provides a new strategy for further designing nanosensors for intracellular or subcellular pH.
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BIOMATERIALS
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2013
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IF 7.604
Wenchuan She,Ning Li,Kui Luo,Chunhua Guo,Gang Wang,Yanyan Geng,Zhongwei Gu
Heparin drug conjugates are currently investigated as excellent candidates for drug delivery vehicles. In this study, we report the preparation and characterization of dendronized heparin–doxorubicin (heparin–DOX) conjugate as pH-sensitive drug delivery vehicle by combination of the features of dendrimer and heparin. Dynamic light scattering (DLS) and transmission electron microscope (TEM) studies demonstrated the dendronized heparin–DOX conjugate self–assembled into compact nanoparticles with negatively charged surface . The nanoparticles with 9.0 wt% (weight percent) of doxorubicin (DOX) showed pH-sensitive property due to the faster drug release rate at pH 5.0 and slow release rate at pH 7.4 aqueous. The nanoparticles were shown to effectively kill cancer cells in vitro . Notablely, the nanoparticles resulted in strong antitumor activity , high antiangiogenesis effects and induced apoptosis on the 4T1 breast tumor model due to the evidences from mice weight shifts, tumor weights, tumor growth curves, immunohistochemical assessment and histological analysis . It's also noteworthy that dendronized heparin and its nanoparticle with drug demonstrated no significant toxicity to healthy organs of both tumor–bearing and healthy mice, which was confirmed by histological analysis compared with free drug DOX. The dendronized heparin–DOX conjugate based nanopatilce with high antitumor activity and low side effects may be therefore a potential nanoscale drug delivery vehicle for breast cancer therapy.
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TALANTA
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2019
·
IF 4.916
Lei Wang,Ying Zhou,Yan Zhang,Guomei Zhang,Caihong Zhang,Yujian He,Chuan Dong,Shaomin Shuang
pH regulates the function of many organelles and plays a pivotal role in requiring multitud cellular behaviors. Compared with single fluorescent probes , ratio fluorescent probes have higher sensitivity and immunity to interference. Herein, a novel Janus ratio nanoprobe was developed for intracellular pH detection. Modified rhodamine B probe and fluorescein isothiocyanate (FITC) were individually encapsulated in the independent hemispheres of Janus microparticles fabricated via Pickering emulsion. Moreover, it exhibits a satasified ratiometric detection of pH compared to the previous core-shell structure and organic small molecule probe. Accordingly, the Janus nanoprobe possesses many important features as an attractive sensor, including high anti-jamming capability, excellent stability, good reversibility and low cytotoxicity. Variations of the two fluorescence intensities (Fgreen/Fred) resulted in a ratiometric pH fluorescent sensor, which can respond to wide range of pH values from 3 to 8. To be more specific, with a single excitation wavelength of 488 nm, there are dual emission bands centered at 538 nm and 590 nm. Also the Janus nanoprobe displays a excellent linear relationship in the physiologically relevant pH range of 4.0–6.0. Consequently, detecting of pH and imaging was successfully achieved in living cells, which provides a simple and reliable method for detecting intracelluar pH and other similar substances.
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MOLECULES
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2022
·Vol. 27
·期 2
·425
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IF 4.927
Jia Luo,Zongyu Guan,Weijie Gao,Chen Wang,Zhongyuan Xu,Chi Meng,Yun Liu,Yuquan Zhang,Qingsong Guo,Yong Ling
Theranostic agents for concurrent cancer therapy and diagnosis have begun attracting attention as a promising modality. However, accurate imaging and identification remains a great challenge for theranostic agents. Here, we designed and synthesized a novel theranostic agentH6Mbased on the “double-locked” strategy by introducing an electron-withdrawing nitro group into 1-position of a pH-responsive 3-amino-β-carboline and further covalently linking the hydroxamic acid group, a zinc-binding group (ZBG), to the 3-position of β-carboline to obtain histone deacetylase (HDAC) inhibitory effect for combined HDAC-targeted therapy. We found thatH6Mcan be specifically reduced under overexpressed nitroreductase (NTR) to produceH6AQ, which emits bright fluorescence at low pH. Notably,H6Mdemonstrated a selective fluorescence imaging via successive reactions with NTR (first “key”) and pH (second “key”), and precisely identified tumor margins with a high S/N ratio to guide tumor resection. Finally,H6Mexerted robust HDAC1/cancer cell inhibitory activities compared with a known HDAC inhibitor SAHA. Therefore, the NTR/pH-activated theranostic agent provided a novel tool for precise diagnosis and efficient tumor therapy.Keywords:β-Carboline;nitroreductase;NTR/pH dual-responsive;HDAC inhibitor;theranostic agent
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Wei-Yi Zhang,Chun-Yan Zhang,Hang-Yu Zhou,Tao Tian,Hua Chen,Hao Zhang,Feng-Qing Yang
Depending on the pH sensitivity of Prussian blue nanoparticles (PB NPs) and the specificity of urease-catalyzed urea, a paper-based colorimetric sensor was developed using urease, PB NPs, and cellulose filter paper. With the assistance of a smartphone, the rapid detection of urea can be realized. Under the optimal conditions, the linear range of the paper-based sensor for detection of urea is 0.5–5.0 mM, and the limit of detection is 0.27 mM. The RSD values of within-batch and between-batch repeatability are 2.5 % and 0.9 %, respectively. The method was successfully applied to the detection of urea in spiked milk samples and artificial urine sample, and the recoveries are in the range of 91.8–107.9%. The developed paper-based colorimetric sensor is a simple, economic, and rapid method that can be used for the determination of urea in real samples, particularly in resource-poor regions.
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