CELL BIOCHEMISTRY AND BIOPHYSICS
·
2014
·Vol. 71
·期 1
·367-373
·
IF 2.38
Gan Maozhou,Yin Xiaodong
This research designed to explore the antitumor activity of puerarin against human mantle cell lymphoma (MCL). Cell proliferation and apoptosis were assessed by MTS and flow cytometry. Caspase-3, -8, and -9 activities were assessed with the colorimetric caspase protease assay. Apoptotic proteins like PARP, cyclin D1, Bcl-2 family, XIAP, and cIAP I were researched by western blot. The PI3K inhibitor LY294002 was used to investigate the possible mechanism relating the PI3K/Akt signaling pathway. Puerarin in vitro inhibited proliferation and induced apoptosis of Z138 cells. Expressions of PI3K and p-Akt were downregulated by puerarin. Puerarin negatively regulated NF-κB activity by inhibiting NF-κB phosphorylation with nuclear translocation inhibition. This kind of effects was correlated with the suppression of expression of cyclin D1, BAX, Bcl-2, XIAP etc. This function was modulated by the PI3K inhibitor. Our results demonstrated that puerarin can induce growth arrest and apoptosis in MCL cells and that the mechanism may involve the NF-κB signaling pathway. Puerarin may have therapeutic applications in the treatment of MCL.
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JOURNAL OF POLYMER RESEARCH
·
2014
·Vol. 21
·期 9
·1-8
·
IF 1.897
Liu Xin,Cheng Jue,Zhang Junying
A facile method is described for preparing thermal plastic polyurethane-polystyrene (PUPS) block copolymer. 2-Hydroxy-2-methyl-1-phenyl-1-propanone (HMPP)-capped polyurethane was synthesized and used as macrophotointiator to prepare PUPS by ultraviolet (UV) irradiation polymerization of styrene. The polymerization process of PUPS block copolymer was monitored by real-time FTIR, 1 H NMR, and GPC, which confirmed the conversion of styrene and formation of PUPS block copolymer. The influence of reaction temperature on styrene conversion was investigated and the results demonstrated the feasibility of preparation of PUPS block copolymer by UV polymerization at ambient temperature. The DSC and TG data revealed a new glass transition temperature and degradable step respectively for PUPS block copolymer induced by polystyrene block compared with neat polyurethane and polyurethane macrophotoinitiator, which further clarified the formation of PUPS block copolymer. The properties measurements showed that the prepared PUPS block copolymer exhibited better tensile strength, hardness and water-resistance than neat polyurethane.
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AMINO ACIDS
·
2014
·Vol. 46
·期 12
·2681-2691
·
IF 3.653
Ruan Zheng,Yang Yuhui,Wen Yanmei,Zhou Yan,Fu Xiaofang,Ding Sheng,Liu Gang,Yao Kang,Wu Xin,Deng Zeyuan,Wu Guoyao,Yin Yulong
Tryptophan (TRP) is an important precursor for several neurotransmitters and metabolic regulators, which play a vital role in regulating nutrient metabolism. The purpose of this study was to investigate the effects of tryptophan supplementation on the biochemical profiles, intestinal structure, liver structure and serum metabolome in rats. Rats received daily intragastric administration of either tryptophan at doses of 200 mg/kg body weight per day or saline (control group) for 7 days. TRP supplementation had a tendency to decrease the body weight of rats ( P > 0.05). The levels of urea and CHO in serum were decreased in the TRP-supplemented group rats compared with control group rats ( P < 0.05). TRP supplementation increased the villus height and the ratio of villus height to crypt depth in the jejunum compared to control group rats ( P < 0.05). Metabolic effects of tryptophan supplementation include: (1) increases in the serum concentrations of lysine, glycine, alanine, glutamate, glutamine, citrulline, methionine, tyrosine, 1-methylhistidine, and albumin, and decreases in the concentrations of serum branched-chain amino acid (isoleucine, valine and leucine); (2) decreases in the serum concentrations of formate and nitrogenous products (trimethylamine, TMAO, methylamine and dimethylamine), and in the contraction of trimethylamine in feces; (3) decreases in serum levels of lipids, low density lipoprotein, very low density lipoprotein, together with the elevated ratio of acetoacetate to β-hydroxybutyrate. The results indicate that tryptophan supplementation reduced the catabolism of dietary amino acids and promoted protein synthesis in rats, promoted the oxidation of fatty acid and reduced fat deposition in the body of rats.
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Science China-Chemistry
·
2014
·Vol. 57
·期 4
·624-632
·
IF 1.516
Zhang Yu,Xiao ChunSheng,Li MingQiang,Ding JianXun,Yang ChenGuang,Zhuang XiuLi,Chen XueSi
A series of well-defined amphiphilic linear-dendritic block copolymers (telodendrimers, MPEG- b -PAMAM-cholesterol) with 1,2,4 or 8 cholesteryl groups (named as P1 , P2 , P4 , P8 , respectively) were synthesized. Their chemical structures were characterized with 1 H NMR and mass spectrum (MALDI-TOF MS). The telodendrimers could self-assemble into micelles in aqueous solution, and encapsulate chemotherapeutic drug doxorubicin (DOX) and paclitaxel (PTX) for combination therapy. All the telodendrimers could encapsulate DOX with similar capability. However, their drug-loading capability of PTX is increased with the increasing number of cholesteryl groups. P8 exhibited much higher PTX loading efficiency than its counterparts. Thus, P8 was selected for further application of drug delivery in the paper. The drug-loading micellar nanoparticles (NPs) of P8 were spherical in shape and their diameters were less than 150 nm which were determined by dynamic light scattering measurements (DLS) and transmission electron microscope (TEM). In vitro drug release experiment demonstrated that P8 exhibited a controlled release manner for both DOX and PTX, and the two drugs were released simultaneously. In vitro cytotoxicity experiment further demonstrated that the co-delivery of DOX and PTX in P8 exhibited better anti-cancer efficiency than the delivery systems encapsulated with single drug (DOX or PTX). This indicates a synergistic effect. The co-delivery system showed potential in future anti-cancer treatment.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2015
·Vol. 20
·期 1
·29-36
·
IF 2.446
Meng Ling,Liu Yingru,Zhang Jingbo,Bai Shouli,Luo Ruixian,Chen Aifan,Lin Yuan
A novel two-phase method was employed to directly deposit PbS quantum dots (QDs) on nanocrystalline SnO 2 thin films. In the two-phase method, the nanocrystalline SnO 2 thin film with adsorption of Pb 2+ ions was placed in an aqueous solution, and S 2− ions were dissolved in an oil solution. As two solutions were contacted, S 2− ions prefer to transfer to the aqueous solution and diffuse to the thin film surface with the adsorbed Pb 2+ ions to homogeneously form monodispersed PbS QDs on the nanocrystalline thin film. The homogeneous monodispersed QDs-sensitized thin film was used as a photoelectrode to fabricate QDs-sensitized solar cell. The loaded amount of PbS QDs and the thickness of ZnS passivation layer were optimized to obtain the highest light-to-electric conversion efficiency of 1.01 % under the simulated AM 1.5 illumination, which is increased by 61 % compared with that of the PbS QDs-sensitized solar cell employing the successive ionic layer adsorption and reaction (SILAR). The solar cell with homogeneous sensitization of QDs generates a photocurrent density of 11.09 mA·cm −2 , which is 2.4 times higher than that of the unhomogeneous one prepared by SILAR. The conversion efficiency enhancement mechanism due to the two-phase method was discussed in detail through the measurements of intensity-modulated photovoltage spectrum and electrochemical impedance spectroscopy.
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MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 15
·2395-2401
·
IF 3.741
Mei He,Sheng Qu,Wu Huimin,Zhang Xiuhua,Wang Shengfu,Xia Qinghua
Alloy nanoparticles of the type Pt x Fe (where x is 1, 2 or 3) were synthesized by coreduction with sodium borohydride in the presence of carbon acting as a chemical support. The resulting nanocomposites were characterized by scanning electron microscopy and X-ray diffraction. The nanocomposite was placed on a glassy carbon electrode, and electrochemical measurements indicated an excellent catalytic activity for the oxidation of glucose even a near-neutral pH values and at a working voltage as low as 50 mV (vs. SCE). Under optimized conditions, the sensor responds to glucose in the 10.0 μM to 18.9 mM concentration range and with a 3.0 μM detection limit (at an S/N ratio of 3). Interferences by ascorbic acid, uric acid, fructose, acetamidophenol and chloride ions are negligible.
Nonenzymatic sensing of glucose is demonstrated at neutral pH values and low working potential using a glassy carbon electrode modified with platinum-iron alloy nanoparticles on a carbon support.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2015
·Vol. 27
·期 1
·1-8
·
IF 2.587
Dong Hua,Tang Guannan,Ma Ting,Cao Xiaodong
In this paper, we report one-step fabrication of poly(lactide-co-glycolic acid)/titanium oxide (PLGA/TiO 2 ) hybrid microspheres with tunable surface texture via droplet-based microfluidics. Surface texture of microspheres can be continuously tuned by changing the mass ratio between titanium tetraisopropoxide (TTIP) and PLGA in the dispersed phase. The fast hydrolysis of TTIP on the droplet surface can generate a thin shell membrane, resulting in a wrinkled surface after extraction of organic solvent. In vitro drug release monitoring of tanshinone IIA-loaded PLGA/TiO 2 hybrid microsphere reveals that surface texture can affect the drug release rate to a large extent without sacrificing the drug encapsulation efficiency. Our finding might benefit the sustained drug delivery where variable drug release rate and high drug encapsulation efficiency are both required.
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CHROMATOGRAPHIA
·
2015
·Vol. 78
·期 11
·833-837
·
IF 1.411
Zhang Mingyu,Qiu Danye,Kang Jingwu
A simple, sensitive and environmentally friendly capillary electrophoresis method for the determination of free sulfate anions in heparin and low-molecular-weight heparin with indirect UV detection is reported. The background electrolyte consists of 20 mM nitrate (pH 5.5) containing 0.2 mM CTAB. The use of nitrate as the probe anion is based on the consideration that nitrate has a very close electrophoretic mobility to that of sulfate, leading to a very narrow peak shape. Moreover, the apparent molar absorptivity of nitrate at 202 nm (8413 L mol −1 cm −1 ) is much higher than that of the commonly used probe anion chromate at 254 nm (2400 L mol −1 cm −1 ). The combination of the narrow peak shape with the high apparent molar absorptivity of the probe ion improved the limit of detection by 2.4 times and the limit of quantitation by 3.2 times. The effect of various CE parameters on the separation of sulfate from chloride was investigated and optimized. The method displays linearity in the range from 0.05 to 20 mM. The RSD % for intraday ( n = 6), and interday ( n = 3) repeatability in terms of migration times and peak areas were all less than 3 %. The limit of detection ( S / N = 3) and limit of quantitation ( S / N = 10) for sulfate were determined as 18 and 53 µM, respectively. The method was successfully applied for the quantification of sulfate anions in heparin and low-molecular-weight heparins.
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MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 7
·1387-1393
·
IF 3.741
Zhang Siqi,Sun Ting,Wang Jiahai
A biomimetic ion-responsive single nanopore sensor was constructed by immobilizing zirconium(IV) ion on a film of poly(ethylene terephthalate) whose surface was modified with polyethyleneimine (PEI) to form nanopores. PEI is an excellent metal-chelating ligand that was initially modified on the surface of the membrane through amidation. The Zr-chelated nanopore responds to inorganic phosphate by a change in the ion current–voltage plot. This is due to a reversal of the charge from positive to negative after binding of the phosphate to the tip of the ion channel. Inorganic phosphate can be quantified by measurement of the extent of rectification. The limit of detection of the method is 118 nM, and response is linear in the 0 to 40 μM phosphate concentration range. The formation of the Zr(IV)/phosphate system on the surface was corroborated by X-ray photoelectron spectroscopy and current–voltage characterization. The single synthetic nanopore display excellent selectivity, high sensitivity and a wide response range for phosphate. The accuracy of the method and applicability of the nanopore-based detection scheme was proven by the successful determination of inorganic phosphate in (spiked) lake water and a cola drink.
The Zr-chelated nanopore responds to inorganic phosphate by a change in the ion current–voltage plot due to a reversal of the charge from positive to negative after binding of the phosphate to the tip of the ion channel. Inorganic phosphate can be quantified by measurement of the extent of rectification. Nanopore-based assay can successful determine inorganic phosphate in (spiked) lake water and a cola drink.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2015
·Vol. 52
·期 12
·8336-8342
·
IF 2.203
Sun Yujing,Zhong Liezhou,Cao Lianfei,Lin Wenwen,Ye Xingqian
Enzyme browning is the main challenge in the preparation of fresh apple juice. The influence of sonication on browning, as well as polyphenols and antioxidant activity of fresh apple juice was investigated. It was found that ultrasound can inhibit the browning of fresh apple ( Malus pumila Mill , cv. Red Fuji ) juice, but decreased the contents of total phenolic content (TPC), total flavonoid content (TFC) and chlorogenic acid and reduced the antioxidant activity. On the whole, ultrasound technology cannot be used to the antibrowning of fresh apple ( Malus pumila Mill , cv. Red Fuji ) juice.
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COLLOID AND POLYMER SCIENCE
·
2015
·Vol. 293
·期 9
·2625-2634
·
IF 1.865
Li Ping,Yang Xiaoyi,Guo Chaohua,Wang Guoyong,Zhang Wei
The adsorption and aggregation behavior of three tetrasiloxane surface active room temperature ionic liquids, N -(2-aminoethyl)-3-aminopropyltetrasiloxane carboxylic acid ammonium, [Si(4)N(2)-2CA(n) ( n = 1, 2, 3)], have been studied on the basis of static/dynamic surface tension, conductivity, dynamic light scattering (DLS), and transmission electron microscopy (TEM). The static surface tension of the Si(4)N(2)-2CA(n) aqueous solutions measured at the critical aggregation concentration (CAC) is observed to be significantly lower than that of amide-functionalized ILs and is comparable to those reported for trisiloxane RT-SAILs, N -(2-aminoethyl)-3-aminopropyltrisiloxane carboxylic acid ammonium and 3-aminopropyltrisiloxane carboxylic acid ammonium. The dynamic surface tension results indicate that adsorption process is a mixed diffusion-kinetic adsorption mechanism. DLS and TEM analysis of Si(4)N(2)-2CA(n) solutions reveal that they can self-assemble into vesicles with an average diameter in the range of 70–500 nm.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 29
·7705-7714
·
IF 3.578
Huang X. Y.,Fang X. W.,Zhang X.,Dai X. M.,Guo X. L.,Chen H. W.,Luo L. P.
Herein, we constructed a platform of neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) for direct and rapid detection of chloramphenicol (CAP) in honey samples diluted with methanol. Under the optimized working conditions, the quantitative information of CAP residues was acquired effectively by EESI-Ion Trap MS n . Using heated methanol-N 2 as spray reagent, we reduced the limit of determination (LOD) from 73.3 ng/mL to 0.3 ng/mL, and the CAP detection is linear in the range of 1–5000 ng/mL ( R = 0.9947). For the honey samples with CAP of 10, 100, and 1000 ng/mL, the recoveries were 133.0, 80.6, and 101.1 %, and the relative standard deviations were 5.96, 8.82, and 8.71 %, respectively. The reproducibility assays showed the stability of this method. Therefore, this ND-EESI-MS method is powerful for direct, rapid, and quantitative CAP analysis in honey samples with high sensitivity, precision, and specificity.
In the current neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) method, N 2 is inlet into samples to desorb chloramphenicol (CAP). We tried to use some organic solvents as the spray reagent to dissolve CAP, and then the best neutral desorption efficiency was obtained when using methanol. We applied this modified ND-EESI-MS method to detect CAP in honey samples only with sample dilution. The limit of CAP detection was then reduced from 73.3 to 0.3 ng/mL, reaching the current EU standard. Therefore, this is a powerful method for direct, rapid, and quantitative CAP analysis in honey samples.
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