FISH & SHELLFISH IMMUNOLOGY
·
2020
·
IF 0
Hairong Wu,Liangliang Mu,Xiaoxue Yin,Kailiang Han,Fangfang Yan,Enxu Zhou,Biao Han,Zheng Guo,Jianmin Ye
Microfibril-associated glycoprotein 4 (MFAP4), a pattern recognition-like molecule with a fibrinogen-like domain (FBG), has the ability to combine and agglutinate pathogens, playing an essential role in the first line of innate immune defense. In this study, the sequence of Nile tilapia (Oreochromis niloticus) microfibril-associated glycoprotein 4 (OnMFAP4) open reading frame (ORF) was amplified and identified. The ORF of OnMFAP4 is 720 bp of nucleotides and codes for 239 amino acids. Spatial mRNA encoding analysis indicated that OnMFAP4 was highly produced in liver, intestine and head kidney in healthy tilapia, and with the lowest expression in muscle. After challenges with Streptococcus agalactiae ( S. agalactiae ) and Aeromonas hydrophila ( A. hydrophila ), the expression of OnMFAP4 mRNA was prominently produced in the liver, spleen and head kidney. The up-regulation of OnMFAP4 expression was also presented in head kidney monocytes/macrophages (MO/MΦ) and hepatocytes. Recombinant OnMFAP4 ((r)OnMFAP4) could bind and agglutinate both bacterial pathogens. Moreover, (r)OnMFAP4 could take part in the modulation of inflammation and phagocytosis . In conclusion, this study revealed that OnMFAP4 might take effect in host defense against bacterial infection in Nile tilapia , with agglutination and opsonization capability to bacterial pathogens.
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ARCHIVES OF MICROBIOLOGY
·
2025
·Vol. 208
·期 1
·1
·
IF 2.6
Zhong Huichang,Chen Liyi,Liu Weifeng,Tao Yong
Xanthophyllomyces dendrorhous (anamorph form Phaffia rhodozyma ) is a key microbial host for industrial astaxanthin production, where yield depends on both the efficiency of the biosynthetic pathway and overall metabolic robustness. To develop superior industrial strains, we established an efficient evolutionary-enrichment system and an accelerated evolutionary workflow that couples astaxanthin production with cell growth under optimized oxidative stress conditions. This strategy enabled the enrichment of mutants exhibiting both high astaxanthin production and strong industrial fitness. Using this approach, we employed a high astaxanthin-producing X. dendrorhous strain A06 as the parental strain for evolution. After several rounds of screening over 2,000 colonies, we successfully identified the top-performing mutant, TP-4. TP-4 demonstrated a stable 20% increase in astaxanthin production relative to the parental strain, along with faster growth and enhanced fermentation performance. The genome of TP-4 was sequenced, and comparative genomic analysis against the parent strain A06 and a low-astaxanthin-producing control strain, C01, revealed multiple mutations potentially underlying its superior phenotype. These findings provide new insights into the metabolic and genetic basis of astaxanthin overproduction. This work establishes an efficient evolutionary platform for strain improvement and provides a mechanistic understanding of astaxanthin overproduction in X. dendrorhous , laying the groundwork for further optimization and industrial-scale application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiang Pu,Jiahua Zhang,Jinwei He,Zhihui Ai,Xiaoxue He,Xiaojun Zhou,Shiyuan Tong,Xinyue Dai,Qiqi Wu,Jiayu Hu,Jingshu He,Hanguang Wang,Wei Wang,Jinqiu Liao,Li Zhang
The biosynthetic route for flavonol in Camptotheca acuminata has been recently elucidated from a chemical point of view. However, the genes involved in flavonol methylation remain unclear. It is a critical step for fully uncovering the flavonol metabolism in this ancient plant. In this study, the multi-omics resource of this plant was utilized to perform flavonol O -methyltransferase-oriented mining and screening. Two genes, CaFOMT1 and CaFOMT2 are identified, and their recombinant CaFOMT proteins are purified to homogeneity. CaFOMT1 exhibits strict substrate and catalytic position specificity for quercetin, and selectively methylates only the 4′-OH group. CaFOMT2 possesses sequential O -methyltransferase activity for the 4′-OH and 7-OH of quercetin. These CaFOMT genes are enriched in the leaf and root tissues. The catalytic dyad and critical substrate-binding sites of the CaFOMTs are determined by molecular docking and further verified through site-mutation experiments. PHE181 and MET185 are designated as the critical sites for flavonol substrate selectivity. Genomic environment analysis indicates that CaFOMT s evolved independently and that their ancestral genes are different from that of the known Ca10OMT . This study provides molecular insights into the substrate-binding pockets of two new CaFOMTs responsible for flavonol metabolism in C. acuminata .
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高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2024
·
IF 2
Guofang Jiang,Can Zhang,Xiaohong Xing,Jinbo Ouyang,Hong Yang,Zongbo Xie,Zhanggao Le
A novel and highly efficient method for the synthesis of 1-(4-hydroxybutyl)-2-benzoyl indoles from 2-pyrrolidine benzaldehyde and α -bromoacetophenone in a choline chloride (ChCl)/ZnCl 2 eutectic mixture was developed. This method, which does not require any catalyst, additive, or alkali, is useful for realizing tandem cyclization reactions. Several indoles were produced after cyclization and ring opening processes. Not all the compounds obtained are reported in the literature. This synthetic strategy proves that deep eutectic solvents can replace catalysts and organic solvents. Consequently, this is an environmentally friendly strategy that has significant potential in the development of green and sustainable chemistry.
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高影响因子
Crystals
·
2019
·Vol. 9
·期 9
·474
·
IF 2.061
Xunan Shen,Chengchun Tang,Chao Zhang,Guanda Li,Yue Zhao,Wei Li,Guifeng Chen,Tie Yang
In current work, Cu2ZnSn(S,Se)4thin films have been prepared by the sol-gel method based on dimethyl sulfoxide solution followed by a modified three-step selenization process. The key process of this method is to divide the Se evaporation and annealing into two different stages: employ a thermal cracking Se source in the Se evaporation stage and an above-atmospheric pressure in the annealing process. The morphological, structural, elemental distributional, and photovoltaic properties of Cu2ZnSn(S,Se)4thin films prepared with the three-step selenization process were systematically investigated. It was found that through this modified selenization process, the formations of secondary phases (ZnSe, CuSnSe3) and a fine-grain bottom layer, which usually exists in the traditional one-step selenization process, were effectively suppressed. These improvements could further reduce the carrier recombination and improve the solar cell performance. The best solar cell is obtained with a short-circuit current density of 28.16 mA/cm2, open-circuit voltage of 404.91 mV, fill factor of 62.91%, and a power conversion efficiency of 7.17% under air mass 1.5 (100 mW/cm2) illumination.Keywords:CZTSSe solar cell;sol-gel method;selenization;thermal cracking Se;above-atmospheric pressure;secondary phases
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高影响因子
RSC Advances
·
2021
·Vol. 11
·期 6
·3470-3475
·
IF 3.361
Ye Meng,Weiwei Zhao,Jun Zheng,Daofa Jiang,Jie Gao,Yanmei Jin,Peihua Ma
Since the first reportal on decamethylcucurbit[5]uril (Me10Q[5]) in 1992, substituted cucurbit[n]urils have attracted considerable research interest. In this study, the host–guest modes between the tetramethyl cucurbit[6]uril (TMeQ[6]) as a host and 4-chloroaniline and 4,4′-diaminostilbene (G1 and G2) as guests were investigated by single-crystal X-ray diffraction, NMR, ITC, UV-Vis spectrum, and MALDI-TOF mass spectrometry analyses. The experimental results showed that TMeQ[6] formed a 1 : 1 inclusion compound with G1, and the carbonyl portal of TMeQ[6] formed a 1 : 1 self-assembly with G2. Further, multi-dimensional supramolecular frameworks were formed driven by weak interaction forces in the system (hydrogen bonding, C–H⋯π interactions, ion–dipole interactions, and dipole–dipole interactions).
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JOURNAL OF POWER SOURCES
·
2021
·
IF 9.127
Zhaoyu Sun,Hebing Zhou,Xuehuan Luo,Yanxia Che,Weishan Li,Mengqing Xu
A novel electrolyte additive designed as a cathode interface formation enhancer for high voltage lithium-ion battery (LIB) systems is investigated. Lithium 4-benzonitrile trimethyl borate (LBTB) is synthesized by a two-step reaction, and the results show that the cycling performance of Li/LiCoO 2 cell at 4.4 V is significantly improved from 15.08% to 73.21% by adding 2.0 wt% of LBTB to the electrolyte (1.0 M LiPF 6 in EC/DEC/EMC = 3/2/5, wt.%). A combination of electrochemical methods , ex-situ spectroscopy characterization techniques and computational calculations are employed to investigate the mechanisms of action for this additive. Cyclic voltammograms indicate the additive is preferably oxidized on the LiCoO 2 surface prior to the electrolyte oxidation and results in a more stable and robust passivation layer than the electrolyte alone. Computational calculations support the observed sacrificial oxidation of LBTB prior to the electrolyte solvents decomposition. The CEI formed by LBTB decomposition mitigates the dissolution of transition metal and subsequently improves the cycling stability of the LiCoO 2 cathodes at high voltages. Surface characterization techniques reveal the presence of -B-O- products derived from LBTB decomposition on the surface of the LCO cathode.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Hanxun Qiu,Feilong Qiu,Xuebin Han,Jing Li,Junhe Yang
Here we report an environmentally friendly, one-pot strategy toward preparation of nickel nanoparticle-decorated reduced graphene-oxide (Ni-RGO) nanostructures, by employing Ni(AC) 2 as nickel source and ethylene glycol as both solvent and reducing agent via a facile microwave irradiation heating approach. The results show that Ni nanoparticles with an average diameter of around 40 nm are homogeneously anchored onto the surface of RGO sheets. As compared to the pure Ni nanoparticles and RGO sheets, Ni-RGO composites with over 64 wt% loading of Ni nanoparticles possess superior catalytic activities and selectivity toward the reduction of 4-nitrophenol. The corresponding kinetic reaction rate constant (defined as κ ) is even four-fold compared to pure Ni nanoparticles. Such promising composites show great potential for friendly treatment of industrial waste containing nitrophenol in a simple, sustainable and green way.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2010
·
IF 3.083
Jiadong Huang,Xiuming Zhang,Su Liu,Qing Lin,Xiaorui He,Xianrong Xing,Wenjing Lian,Di Tang
4-Nonylphenol (4-NP) was reported to affect the health of wildlife and humans through altering endocrine function. A novel electrochemical sensor for sensitive and fast determination of 4-NP was developed. Titanium oxide (TiO 2 ) nanoparticles and gold nanoparticles (AuNPs) were introduced for the enhancement of electron conduction and sensitivity. 4-NP-imprinted functionalized AuNPs composites with specific binding sites for 4-NP was modified on electrode. The resulting electrodes were characterized by cyclic voltammetry (CV). Rebinding experiments were carried out to determine the specific binding capacity and selective recognition. The linear range was over the range from 4.80 × 10 −4 to 9.50 × 10 −7 mol L −1 , with the detection limit of 3.20 × 10 −7 mol L −1 ( S / N = 3). The sensor was successfully employed to detect 4-NP in real samples.
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高影响因子
DALTON TRANSACTIONS
·
2024
·Vol. 53
·期 9
·4325-4341
·
IF 3.5
P. J. Xia,X. Z. Zheng,L. Yue,Y. F. Lei,M. Xu,W. B. Dai
For further development of light sources, white light-emitting diodes (wLEDs) have attracted widespread attention as promising next-generation light sources fabricated via the combination of phosphors and LED chips. However, latent defects, such as chemical/thermal instability, low color rendering index (CRI) and high correlated color temperature (CCT), of current mainstream wLEDs seriously hinder their further large-scale implementation. Herein, in order to overcome these limitations, single-phase color-tunable gaudefroyite (Ca3Y(GaO)3(BO3)4 (CYGB)) tridoped with Bi3+/Tb3+/Eu3+ ions was synthesized for the first time and detailed characterisation was performed via high-temperature solid-state reaction and structural/spectral analyses, respectively. Radius difference percentage calculations and Rietveld refinements indicate that dopants occupy both Y3+ and Ca2+ sites but preferably the Y3+ site over the Ca2+ site due to the same valence state. Through subtly regulating the (co)doping contents and skillfully utilizing the energy transfer (ET) strategy from the allowed transition of blue light-emitting Bi to the forbidden transition of green/red light-emitting Tb/Eu, the color hue (including white light) of highly efficient PL can be easily tuned according to the need. Meanwhile, composition/content-optimized white light-emitting CYGB:2%Bi/10%Tb/12%Eu also shows splendid chemical/thermal stability. Finally, as a proof-of-concept experiment, the CYGB:2%Bi/10%Tb/12%Eu phosphor-converted wLED (pc-wLED) was fabricated and encapsulated via the up-to-date remote ‘capping’ method, which imparted attractive performances. Altogether, the stable CYGB:Bi/Tb/Eu phosphor is a promising candidate for application in lighting/display fields.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Wanyu Gao,Yuan Gao,Ziping Yin,Yanfei Sun,Yu Zhang,Longyi Zhu,Jun Luo
The solubility of 3,4-bis(4′-aminofurazan-3′-yl)furoxan (BAFF) was determined in ten pure solvents (dichloromethane, 1,2-dichloroethane, cyclohexane, water, methanol, ethanol, n-propanol, isopropanol, n-butanol, and ethyl acetate) as well as three binary solvent mixtures (methanol/water, ethanol/water, ethyl acetate/1,2-dichloroethane) over various temperature ranges (283.15 to 353.15 K). The measurements were conducted by using a static analytical method under atmospheric pressure. During the temperature ranges investigated, it was observed that the solubility of BAFF increased with the increase in the temperature in all selected solvents. Among the ten pure solvents, ethyl acetate has the highest solubility, while methanol, ethanol, n-butanol, and isopropanol have moderate solubilities. Water, 1,2-dichloroethane, and dichloromethane have relatively low solubilities, while in the binary solvent systems, the solubility of BAFF decreased with increasing the mole fraction of antisolvent (water or 1,2-dichloroethane) at given temperature. Moreover, the experimental solubility data in pure solvents were correlated using the modified Apelblat equation, the λh equation, and two local composition models (Wilson model and NRTL model). Additionally, the modified Apelblat equation, CNIBS/R-K model, Jouyban-Acree model, and multicomponent NRTL model were successfully employed to correlate the experimental solubility in mixed solvent systems. Furthermore, based on the experimental data and the NRTL model, the dissolution thermodynamic properties of BAFF in both pure and binary solvents were calculated and analyzed. These properties include the dissolution Gibbs energy, enthalpy, and entropy. These data indicate that the dissolution process of BAFF is endothermic and spontaneous.
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Nanoscale
·
2024
·Vol. 16
·期 36
·16933-16941
·
IF 5.8
Xinyu Feng,Siwen Zhang,Jiazhuo Li,Yingfang Hu,Rongyuan Ge,Yaowen Shi,Yali Yao,Bosi Yin,Tianyi Ma
Ni3(NO3)2(OH)4 has a high theoretical specific capacitance, low cost, and environmental friendliness, making it a promising electrode material. Specifically, Ni3(NO3)2(OH)4 electrodes have a larger layer spacing (c = 6.898 Å) than Ni(OH)2 electrodes since NO3− has a much larger ionic radius than OH−. The larger layer spacing stores more electrolyte ions, significantly improving the electrochemical activity of the electrodes. Additionally, the interlayer NO3− can enhance the structural stability of Ni3(NO3)2(OH)4. However, since Ni3(NO3)2(OH)4 has a higher molar mass than Ni(OH)2, it has a lower theoretical specific capacity. Consequently, Ni3(NO3)2(OH)4 has not been used in zinc-based alkaline batteries. Studies showed that doping could enhance the electrochemical performance of electrode materials. Therefore, this study used a simple solvothermal reaction to synthesize yttrium-doped Ni3(NO3)2(OH)4 (Y-Ni3(NO3)2(OH)4), assembling a Y-Ni3(NO3)2(OH)4//Zn battery for electrochemical testing. Y-Ni3(NO3)2(OH)4 served as the cathode in the battery. The analysis of Y-Ni3(NO3)2(OH)4 showed that yttrium (Y) doping increased the specific surface area and pore size of Ni3(NO3)2(OH)4 significantly. The increased specific surface area improved the active material utilization, and the abundant mesopores facilitated OH− transport, substantially enhancing the battery's specific capacity and energy density. Ultimately, the specific discharge capacity of the advanced Y-Ni3(NO3)2(OH)4//Zn battery reached 177.97 mA h g−1 at a current density of 4 A g−1, nearly doubling the capacity of the earlier Ni3(NO3)2(OH)4//Zn battery (103.59 mA h g−1).
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高影响因子
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