SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Meng-Meng Liu,Shan-Hong Li,Dan-Dan Huang,Zhi-Wei Xu,Ya-Wei Wu,Yun Lei,Ai-Lin Liu
Diagnostic assays made of microfluidic paper-based analytical devices have become the focus of considerable attention in developing countries because of simplicity, low cost, user friendliness, and no requirement for complex equipment, which is suitable to point-of-care testing. Considering the intrinsic shortcomings of nature enzyme, synthesis of artificial peroxidase mimics possessing improved catalytic activities relative to native enzymes is of considerable interest for the development of highly sensitive and stable sensor. In this paper, water-soluble molybdenum oxide quantum dots (MoOx QDs) were developed as highly effective biomimetic catalysts which were prepared by a facile ultrasonic-assisted hydrothermal method. The resulted MoOx QDs displayed peroxidase-like activity with high sensitivity and specificity, and were constructed for efficient colorimetric quantitative detection of H 2 O 2 based on microfluidic paper-based device. As low as 0.175 μmol/L H 2 O 2 could be determined at a linear range from 1 to 20 μmol/L. Moreover, this biosensing device was successfully applied for visual detection of H 2 O 2 released from PC12 cells with the advantages of low cost, rapid response and portability, indicating that the designed sensor of H 2 O 2 has great potential in biotechnology and clinical diagnosis.
研究领域:
生物传感
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医学诊断
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材料科学
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DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
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2022
·Vol. 51
·期 8
·4493-4508
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IF 2.047
Chen Yuan Tao,Wang Fang,Zhang Min,Zeng Han
2-Methyl imidazole and Zn(NO 3 ) 2 were proposed as raw materials for the hemoglobin (Hb) accommodation matrix ZIF-8, and the encapsulation of Hb molecules onto ZIF-8 was achieved by hydrogen bond interaction and electrostatic attraction. The electrochemical sensing performance of H 2 O 2 for Hb integration into metal–organic framework (MOF) material was analyzed systematically and evaluated quantitatively to identify the key factor in limiting the sensing efficiency. The influence of mutual interactions between Hb and ZIF-8 on the spectroscopic features and sensing efficiency for complexes with Hb incorporation was estimated by multiple techniques to explore the impact of those interactions on the relevant steps involved in the catalysis. The results from experiments revealed that the hydrogen bond between Hb and ZIF-8 led to non-oriented aggregation of enzyme molecules onto the MOF with reduced active surface area and inhibited catalytic activity. The relatively weak interaction between heme protein and MOF material did not result in serious distortion in the structure or chemical nature of Hb entrapment into ZIF-8. The presence of a mutual interaction between Hb and ZIF-8 did not impose a serious negative effect on heterogeneous electron shuttle (28.2–91.2 s −1 ) for the heme site in Hb entrapment into ZIF-8, which was classified into a typical quasi-reversible and thin-film confining mode with the involvement of four electrons and two H + ions. The results showed that hb-induced electrocatalysis was limited by the step of substrate diffusion (1.3 × 10 −3 s −1 ). The Hb-based electrode demonstrated excellent affinity to dissolved H 2 O 2 (Michaelis constant: 28.0 μM) and desirable sensing performance (low detection limitation: 3.7 μM). Graphical Abstract Illustration of the procedure for preparing the electrode based on MOF material (ZIF-8) with hemoglobin accommodation via physical adsorption and electrostatic attraction, which gives rise to non-oriented distribution of hemoglobin attachment onto the surface and inner cavity of ZIF-8.
研究领域:
生物化学
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电化学
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材料科学
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DOI
高影响因子
Frontiers of Chemical Science and Engineering
·
2024
·Vol. 18
·期 5
·1-12
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IF 4.3
Meng Yu,Wang Yitong,Li Guozhu,Liu Guozhu,Wang Li
Primary, secondary and tertiary amino-functionalized zirconia (ZrO 2 −NH 2 , ZrO 2 −NH and ZrO 2 −N) was synthesized by the postgrafting method for the adsorption removal of typical metallic ions, phosphate and total oxidizable carbon from a real H 2 O 2 solution. ZrO 2 −NH 2 , ZrO 2 −NH and ZrO 2 −N exhibited similar pore sizes and sequentially increased zeta potentials. The adsorption results of single and binary simulated solutions showed that the removal efficiency increased in the order of Fe 3+ > Al 3+ > Ca 2+ > Na + . There is competitive adsorption between metallic ions, and Fe 3+ has an advantage over the other metals, with a removal efficiency of 90.7%. The coexisting phosphate could promote the adsorption of metallic ions, while total oxidizable carbon had no effect on adsorption. The adsorption results of the real H 2 O 2 solution showed that ZrO 2 −NH 2 exhibited the best adsorption affinity for metallic ions, as did phosphate and total oxidizable carbon, with a total adsorption capacity of 120.9 mg·g −1 . Density functional theory calculations revealed that the adsorption process of metallic ions involves electron transfer from N atoms to metals and the formation of N-metal bonds.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2017
·
IF 6.216
Lele Cui,Peipei Ding,Ming Zhou,Wenheng Jing
Electrochemical technology offers the possibility of in situ hydrogen peroxide generation whose productivity greatly depends on the cathode properties and the reactor configuration. Herein, cerium oxide modified graphite felt is prepared and used as cathode. Ceramic membrane is integrated dividing the cell to two compartments and preventing decomposition of cathode-generated H 2 O 2 at the anode. H 2 O 2 is found almost impermeable through the membrane as concentration difference <0.5 M. The double-cell reactor performs higher energy efficiency than the conventional electrolytic cell. And H 2 O 2 accumulation rate has been enhanced in the double-cell in comparison with the undivided one, no matter using either bared or modified cathode. Operation parameters including flow rate of O 2 ( F O 2 ), flow rate of electrolyte solution (F solution ), applied current (I) and pH were optimized. Accumulated concentration of H 2 O 2 on the modified cathode reached 260.7 mg/L with the current efficiency up to 75.7% (working conditions C Na 2 SO 4 = 0.05 M, pH = 2, I = 50 mA, F O 2 = 0.2 L/min and F solution = 800 μL/min). The yield was 3.8 times higher than using the unmodified cathode. Energy consumption was reduced to 4.8 kWh/kg in the double-cell system (22.1 kWh/kg in the single-cell). In a conclusion, the double-compartment flow reactor is an improved configuration for energy effectiveness and increased productivity of H 2 O 2 for E-Fenton process.
研究领域:
环境工程
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电化学工程
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材料科学
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能源技术
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水处理技术
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反应器设计
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催化材料
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DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
·
IF 14.229
Can Ma,Shuo Feng,Jinming Zhou,Rufen Chen,Yu Wei,Hui Liu,Sen Wang
The greatest problem in the homogeneous Fenton reaction is the low production of HO owing to the slow Fe III /Fe II cycle. In this study, we demonstrate a new co-catalyst of FeP and trace Fe 2+ that can greatly enhance the decomposition efficiency of H 2 O 2 and accelerate the degradation of methylene blue (MB). This co-catalyst is applicable in a broad pH range (pH = 3.0−9.0) and capable of degrading 95.74% and 89.01% of MB with 100 ppm in concentration within 30 s at pH 3 and 5, and 99.65% and 90.06% within 3 min at pH 7 and 9. The coexistence of FeP with Fe II accelerates the Fe III /Fe II cycle by electron transfer between Fe δ+ in FeP and Fe III . Mössbauer spectra and density functional theory calculations indicate that the unique structure of FeP plays an important role in accelerating the Fe III /Fe II cycle.
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DOI
高影响因子
ELECTROANALYSIS
·
2018
·Vol. 30
·期 6
·1047-1053
·
IF 2.851
Shuai Li,Yang Tian
The serious damage of ischemia to brain function has attracted extensive attention. As two of vital signal molecules, development of biosensors for monitoring of hydrogen peroxide (H 2 O 2 ) and pH in vivo is significant to understand the roles of H 2 O 2 and pH playing in rat brain followed by ischemia. Herein, a selective and accuracy ratiometric electrochemical biosensor was developed for quantifying H 2 O 2 and pH through both current and potential outputs in a rat brain upon ischemia. Catalase (Cat) was first employed as a specific recognition element for both H 2 O 2 and pH. Meanwhile, an inner reference molecule, ferrocene (Fc), independent of H 2 O 2 and pH, was used as a built-in correction to improve the accuracy. The reduction peak current of Cat increased with increasing concentration of H 2 O 2 from 1.0 to 230 μM. Meanwhile, the peak potential negatively shifted with increasing pH from 5.91 to 7.81, resulting in determination of H 2 O 2 and pH. Thus, combined with the remarkable analytical performance of the developed biosensor and the desirable biocompatibility of carbon fibre microelectrode (CFME), a direct and reliable approach was established to monitoring H 2 O 2 and pH in the microdialysates collected from rat brain followed by cerebral ischemia.
研究领域:
电分析化学
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神经化学
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生物传感器
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生物医学工程
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DOI
高影响因子
Catalysis Science & Technology
·
2024
·Vol. 14
·期 4
·990-998
·
IF 4.4
Huichao He,Jinyu Wen,Qiwen Chen,Hai Yu,Kun Liu,Tao Han,Xiaoyan Liu,Gaili Ke,Yutong Li,Huaming Yang
The catalytic activation of HCO3−/H2O2 to generate reactive oxygen species could treat neutral or weak alkaline organic wastewater effectively. Based on the activation principle of HCO3−/H2O2 and the advantages of natural tourmaline, four types of common tourmaline powders were investigated as catalysts to activate HCO3−/H2O2 for the degradation of typical anionic (methyl orange: MO) and cationic (methylene blue: MB; rhodamine B: RhB) dyes in aqueous solution. Compared to magnesium-rich tourmaline, lithium-rich tourmaline and low Fe content tourmaline, higher activity was observed for high Fe content tourmaline (high-Fe TM) in the catalytic activation of HCO3−/H2O2 to achieve dye degradation. Furthermore, high-Fe TM exhibited acceptable stability for activating HCO3−/H2O2 for the degradation of dyes, and the co-existing ions in municipal tap water, temperature and pH had a weak influence on the catalytic activity of high-Fe TM. The degradation of dyes in the high-Fe TM/HCO3−/H2O2 system was found to be initiated by typical reactive oxygen species, which are mainly originated from the catalytic activation of HCO3−/H2O2 with high-Fe TM. Specifically, the Fe-based sites of high-Fe TM and the Fe ions dissolved with high-Fe TM co-activated HCO3−/H2O2 through heterogeneous and homogeneous catalysis, respectively. The degradation of RhB was driven by ˙OH and ˙O2−, and the degradation of MB and MO was initiated by 1O2.
研究领域:
环境化学
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催化科学
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水处理技术
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材料科学
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污染控制
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DOI
高影响因子
Chemical & Biomedical Imaging
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2024
·
IF 0
Xing Tian,Jiayi Cheng,Lei Yang,Zhanxian Li,Mingming Yu
Hydrogen peroxide and polarity are closely related to many physiological activities and pathological processes. However, near-infrared fluorescent probes that are sensitive to both H2O2 and polarity are still scarce. Herein, we developed the first dual-channel near-infrared fluorescent probe NBO, with an AIE effect, enabling simultaneous monitoring of H2O2 and polarity. The probe presented high sensitivity, high selectivity, and low detection limit for H2O2. It also had high sensitivity to polarity, independent of pH and viscosity, with large Stokes shifts, good photostability, and low cytotoxicity. Moreover, NBO was able to detect both endogenous and exogenous H2O2 as well as polarity fluctuations in vivo as a method to effectively differentiate between cancer cells and normal cells. Importantly, it also could monitor the therapeutic effects of drugs in inflammation and iron-dead cells and mice. Based on NIR emission, NBO could be used as an imaging tool and a way to evaluate the therapeutic effect of drugs for inflammation and ferroptosis.
研究领域:
生物化学
·
细胞生物学
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药理学
·
分子成像
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DOI
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Dandan Fang,Xiangqin Zheng,Huan Yi,Hong Dai,Zhensheng Hong,Yiru Peng,Yanyu Lin
A highly sensitive electrochemiluminescence (ECL) immunosensor was fabricated for the quantitative analysis of deoxynivalenol (DON) based on an effective all-in-one bioprobe that composed of TiO 2 -B with Fluoro-coumarin silicon phthalocyanine (F-couSiPc) and luminol. Herein, TiO 2 -B with large specific area was served as a favorable carrier, greatly increased the loading of enhancer F-couSiPc and luminophore luminol, resulting in the tremendous enhancement of ECL signal. Specifically, F-couSiPc as coreaction accelerator could facilitate the decomposition of dissolved O 2 to generate substantial reactive oxygen species (ROS) to participate in the ECL reaction of luminol. Additionally, the prepared PEDOT/HA@mp MnO 2 was employed as the sensing platform of biosensor, which can not only capture substantial antibody to improve the sensitivity, but also could expedite the electronic transmission rate to make the ECL intensity further amplified. Ultimately, the proposed immunosensor presented favorable performance for the determination of DON, such as high sensitivity, good stability, wide liner range (from 1.0*10 −6 ng/mL to 10 ng/mL) and a low detection limit of 3.3*10 -7 ng/mL.
研究领域:
分析化学
·
食品安全检测
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DOI
高影响因子
ACS Applied Electronic Materials
·
2026
·Vol. 8
·期 15
·6545-6553
·
IF 4.7
Liu Qianzuo,Sun Sifan,Li Jiawei,Yang Haoran,Xiang Xinyue,Hao Ying,Yan Xuehua,Zhang Ting,Li Tie
Hydrogen peroxide (H 2 O 2 ), a key reactive oxygen species in physiological and pathological processes, demands highly sensitive detection for biological and biomedical research, yet developing electrocatalytic materials with high sensitivity, low detection limit, and excellent stability remains a challenge. Herein, a Fe−C@Fe/Ce−C bilayer heterojunction composite was fabricated for H 2 O 2 detection. Benefiting from its unique heterojunction architecture and synergistic Fe/Ce/carbon interactions, the as-prepared nanocomposite exhibits the exceptional H 2 O 2 sensing performance, with a wide linear range of 0.5−6000 µM, a high sensitivity of 490.5 μA·mM −1 ·cm −2 , a low detection limit of 0.12 µM, excellent anti-interference selectivity against common biological interferents, and outstanding long-term stability with no obvious performance variation after 11 days of continuous testing. The robust built-in electric field arising from the heterojunction facilitates interfacial charge transfer and accelerates H 2 O 2 activation, boosting sensing behavior. Furthermore, the flexible electrochemical sensor was fabricated via the screen-printing technology, which enables reliable real-time monitoring of intracellular H 2 O 2 released from HeLa cells, showing great potential for biological sensing. This work provides a promising design strategy for high-performance flexible electrochemical sensors and deepens the understanding of heterojunction and built-in electric field roles in electrocatalytic sensing systems.
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Cai-Yun Li,Xiao-Qian Duan,Xin Yu,Yu-Bin Zhang
500 μmol/L H 2 O 2 significantly enhances beef color stability during postmortem aging. • Moderate H 2 O 2 improves antioxidant capacity and maintains mitochondrial ETC activities. • GFM1 and LDHB coordinate mitochondrial maintenance and glycolysis for color stability. This study investigated the dose-dependent effects of exogenous H 2 O 2 (300, 500, and 1000 μmol/L) on color stability, mitochondrial function, and proteomic profiles in postmortem beef. Among the concentrations tested, 500 μmol/L H 2 O 2 most effectively enhanced color stability, as reflected by increased redness and oxymyoglobin proportion and decreased metmyoglobin formation. This treatment also promoted antioxidant enzyme activities, attenuated pH decline, and maintained mitochondrial electron transport chain complex activities. Quantitative proteomic analysis revealed that these beneficial effects were orchestrated by coordinated upregulation of metabolic pathway and HIF-1 signaling proteins, with mitochondrial translation factor GFM1 and lactate dehydrogenase B (LDHB) emerging as central hubs in a protein-protein interaction network. These findings suggest a previously unrecognized adaptive mechanism coupling mitochondrial maintenance, functional preservation, and glycolytic flux regulation under oxidative stress.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
·
IF 6
Chudong He,Yuanyuan Zhao,Mengdie Mo,Yuhong Su,Zhongquan Qi,Xiao Dong Chen,Weijie Zhou,Fei Yu
Ferroptosis is an iron-dependent programmed cell death modality characterized by the massive accumulation of lipid peroxides (LPO). However, the efficiency of ferroptosis is commonly limited by insufficient supply of iron ions and endogenous hydrogen peroxide (H 2 O 2 ) in the tumor cells. To overcome these drawbacks, MIL-100 NPs were synthesized via a microwave-assisted method to encapsulate β-lapachone (Lap), followed with the hyaluronic acid (HA) surface modification (designated as LMH NPs). LMH NPs exhibited GSH-responsive degradation in the tumor cells to release Lap and Fe 3+ . The released of Lap could generate H 2 O 2 via catalysis by tumor-overexpressed quinone oxidoreductase 1 (NQO1), leading to increased intracellular H 2 O 2 levels. The abundant production of H 2 O 2 could supply substrates for Fe 2+ -mediated Fenton reactions to produce toxic hydroxyl radicals (·OH), thereby promoting LPO accumulation and enhancing ferroptosis. Meanwhile, Fe 3 ⁺ released from LMH NPs was reduced to Fe 2+ through GSH consumption, which could diminish glutathione peroxidase 4 (GPX4) activity. In vitro and in vivo experiments confirmed that LMH NPs possessed effective targeted uptake and exhibited optimal tumor inhibition without significant toxic side effects. Overall, the integrated strategy presents a novel ferroptosis-based clinical approach for enhanced breast cancer therapy. Scheme 1 Preparation process and antitumor mechanisms of LMH NPs. (A) The preparation process of LMH NPs. (B) Antitumor mechanism of LMH NPs through inducing ferroptosis. (C) Lap undergoes a ‘‘futile redox cycling’’ process catalyzed by NQO1. Download: Download high-res image (119KB) Download: Download full-size image
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）