NEUROCHEMICAL RESEARCH
·
2025
·Vol. 50
·期 2
·1-13
·
IF 3.8
Wang Jun,Zhang Nan,Liu Hong-Zheng,Wang Jin-Liang,Zhang Yong-Bo,Su Dong-Dong,Zhang Li-Min,Li Bao-Dong,Miao Hui-Tao,Miao Jun
Neuropathic pain (NP) imposes a significant burden on individuals, manifesting as nociceptive anaphylaxis, hypersensitivity, and spontaneous pain. Previous studies have shown that traumatic stress in the nervous system can lead to excessive production of hydrogen sulfide (H 2 S) in the gut. As a toxic gas, it can damage the nervous system through the gut-brain axis. However, whether traumatic stress in the nervous system leading to excessive production of H 2 S in the gut can ultimately cause neuropathic pain through the gut-brain axis remains to be investigated. This study established a model of chronic constriction injury (CCI) in mice to determine its effects on gut H 2 S production, the associated damage via the gut-brain axis, the potential neuropathic pain, as well as the probable mechanism. A CCI mouse model was developed using a spinal nerve ligation approach. Subsequently, the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were used to determine the mice’s pain thresholds. A variety of assays were performed, including immunofluorescence, western blotting, real-time quantitative Polymerase Chain Reaction (PCR), and membrane clamp whole-cell recordings. Mice subjected to CCI showed decreased MWT and TWL, decreased ZO-1 staining, decreased HuD staining, increased Glial fibrillary acidic protein (GFAP) staining, increased expression of tumor necrosis factor-alpha (TNF-α) protein and interleukin-6 (IL-6) protein, increased expression of NMDAR2B (NR2B) protein and NR2B mRNA, increased colocalization of vGlut2- and c-fos-positive cells, and a higher amplitude of evoked excitatory postsynaptic potential (EPSP) compared to Sham group. These changes were significantly reversed by H 2 S inhibitor treatment, and the specific NMDA receptor inhibitor MK-801 effectively restored the neurotoxicity of H 2 S. H 2 S is involved in CCI-induced neuropathic pain in mice, which might be mediated by the activation of the NMDA signaling pathway.
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CELLULOSE
·
2024
·
IF 4.9
Zhao Wenjing,Sui Zhihui,Zhang Qi,Sun Lijian,Zu Bin
Cellulosic-based materials such as hemp fabric and paper have combined characteristics of renewability, biodegradability, flexibility, and recyclability. However, regular products are not suitable for use in unconventional applications due to inherent limitations, and accordingly, enabling new capabilities is a necessity. To this end, the conversion of regular hemp fabric and paper into high-strength products with hydrophobic function via surface engineering was explored. The fluorinated waterborne polyurethane (FWPU) emulsions were first synthesized as functional coatings by in-situ polymerization. The structure and properties of the FWPU emulsion and film were characterized by FTIR, XRD, SEM, TG, and XPS. The results showed that the grafting of perfluorooctanol imparts WPU film excellent hydrophobicity, and the water contact angle increases from 71.2° to 105.9°. After the FWPU coating, the water contact angles of the hemp fabric and paper increase from 0° to 117.1° and 112.8°, respectively. Quite encouragingly, the coating generation of waterborne polyurethane-derived functional additives led to the increase of mechanical strength by more than 23% and 82%, in the case of qualitative filter paper and hemp fabric. Meanwhile, the folding strength of filter paper is increased by 132 times. Overall, this functionalized waterborne polyurethane coating would direct paper and hemp fabric toward diversified applications.
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Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Chen Fengming,Wu Shu,Wang Xiaoyu,Li Yinghui,He Binsheng,Fu Yafen,Huang Xingguo,Chen Jiayi
Reducing the negative impact of oxidative stress (OS) on weaned piglets is crucial for the further development of the pig industry. Our previous studies found that Lactobacillus delbrueckii (LAB) can alleviate OS and protect intestinal health by activating TLR2 and TLR4 expressions in weaned piglets. This research aimed to verify whether LAB can alleviate OS and intestinal barrier injuries in IPEC-J2 cells by activating TLR2 and TLR4. IPEC-J2 cells were first pretreated with 10 10 CFU/mL LAB for 12 h, and then were induced to OS by 0.8 mmol/L H 2 O 2 for another 12 h. The results showed that LAB significantly alleviated OS induced by H 2 O 2 , indicated by the downregulation of MDA ( P < 0.05), along with upregulation of GSH-Px, CAT, T-SOD, and T-AOC ( P < 0.05). Besides, LAB significantly alleviated intestinal barrier injuries induced by H 2 O 2 , indicated by the upregulation of Claudin-1, Occludin, and ZO-1 expressions. Subsequently, the IPEC-J2 cells were pretreated with inhibitors of TLR2 (TLR2-IN-C29) and TLR4 (MD2-TLR4-IN-1) in the absence of LAB for 12 h, and then treated with 0.8 mmol/L H 2 O 2 for another 12 h. The results showed that both inhibitors significantly prevented the protection effects of LAB on H 2 O 2 -induced OS, indicated by the downregulation of CAT, SOD1, HO-1, and GSH-Px1 expressions ( P < 0.05). Taken together, LAB treatment can alleviate OS and intestinal injuries induced by H 2 O 2 by activating TLR2 and TLR4 expressions in IPEC-J2 cells, thus providing novel theoretical support for LAB in the prevention and treatment of oxidative damage.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-11
·
IF 5.3
Mei Lin,Shi Yanmei,Ding Xueke,Li Jing
Surface functionalization and the combined utilization of zero‐dimensional and two‐dimensional nanomaterials is an effective method to achieve highly sensitive detection for electrochemical analysis. Using an all-in-one strategy, phthalocyanine, gold nanoparticles, and ionic liquid were successively modified on the graphene surface as a highly integrated electrode modification material. Phthalocyanine can repair the defects of reduced graphene oxide by binding to the graphene structure surface through non-covalent functionalization. The combination of ionic liquid on the surface of gold nanoparticles can enhance their physical and chemical activity while preserving their stability. The obtained phthalocyanine-coated graphene nanosheets decorated with ionic liquid-functionalized gold nanoparticles nanocomposites had enhanced electrocatalysis and conductivity ability, and were used for highly sensitive electrochemical detection of ascorbic acid in fruit juices. Excellent results were obtained for the detection of ascorbic acid in the linear range 0.05 to 50 µmol/L with a detection limit of 6.80 nmol/L ( S/N = 3) and a sensitivity of 2.68 μA μM −1 cm −2 , indicated that the proposed sensor strategy with multiple signal amplification can achieve higher detection sensitivity. Furthermore, the sensor was used to quantify ascorbic acid content in grapefruit and orange juice with good selectivity and accuracy. The highly integrated electroactive nanocomposites construction method described may also be used with other electrode modification materials and is anticipated to yield fresh perspectives on the advancement of ultrasensitive detection. Graphical
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-12
·
IF 5.3
Zhou Peipei,Hong Yangluchen,Fan Ru,Wang Huili,Wang Xuedong,Mei He
To realize the reutilization of waste Myrica rubra in the analytical field, we synthesized Myrica rubra –based N-doped carbon dots (MN-CDs) and further anchored them onto the surface of Fe 3 S 4 to fabricate Fe 3 S 4 @MN-CD nanocomposites. The as-fabricated nanocomposites possessed higher peroxidase-mimetic activity than its two precursors, resulting from the synergistic effect between them, and could catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into deep blue oxTMB with a strong 652-nm absorption. Under optimized conditions (initial solution pH, 3.5; incubation temperature, 35 ℃; Fe 3 S 4 @MN-CD concentration, 50 µg mL −1 , and 652-nm absorption), Fe 3 S 4 @MN-CDs were employed for colorimetric assay of p -aminophenol ( p -AP) with wide linear range (LR, 2.9–100 µM), low detection limit (LOD, 0.87 µM), and satisfactory recoveries (86.3–105%) in environmental waters. Encouragingly, this colorimetric assay provided the relative accuracy of 97.0–99.4% as compared with conventional HPLC-UV detection. A portable smartphone-based colorimetric application was developed by combining the Fe 3 S 4 @MN-CD-based visually chromogenic reaction with a “Thing Identify” APP software. Besides, we engineered an image-capturing device feasible for field use, in which the internal-compact sealing prevented external light source from entering photography chamber, thereby reducing light interference, and also the bottom light source enhanced the intensity of blue imaging. This colorimetric platform exhibited satisfactory LR (1–500 µM), low LOD (0.3 µM), and fortification recoveries (86.6–99.6%). In the chromogenic reaction catalyzed by Fe 3 S 4 @MN-CDs, ·O 2 − played a key role in concomitant with the participation of •OH and h + . Both the colorimetric assay and smartphone-based intelligent sensing show great promising in on-site monitoring of p -AP under field conditions. Graphical Abstract
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Nano-Micro Letters
·
2024
·Vol. 16
·期 1
·1-14
·
IF 31.6
Li Cuiping,Li Dan,Zhang Shuai,Ma Long,Zhang Lei,Zhang Jingwei,Gong Chunhong
The boosted heterogeneous interfaces in titanium nitride (TiN) nanotube/polydimethylsiloxane (PDMS) composite contributed to strong polarization loss relaxation ability.
The TiN nanotubes/PDMS composite possessed both good impedance matching behavior and strong dielectric loss ability in wide temperature spectrum.
The TiN nanotubes/PDMS composite exhibited excellent EMWA performances (effective absorption bandwidth value of 3.23 GHz and minimum reflection loss value of − 44.15 dB) at the varied temperature from 298 to 573 K.
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Waste and Biomass Valorization
·
2024
·
IF 2.6
Li Yalin,Liu Lei,Zhang Xing,Dong Tingting,Wang Zhaojun,Xiong Manxin,Zhao Jingyi
The increasing amount of food waste and its sustainable management are gradually becoming global issues. The sustainable management of food waste has great potential benefits in terms of emission reduction and economic benefits. This study reports a novel environmentally friendly kitchen waste hydrogel synthesized from kitchen waste prepared from acrylamide, N,N ′-methylene bisacrylamide, Urea, and sodium persulfate by free radical cross-linking polymerization. Further, the effects of single factors on the solubility and mechanical properties of the hydrogel were investigated. Additionally, using a response surface methodology, the combined effects of the three key experimental parameters-cross-linking agent, temperature, and initiator were investigated on the mechanical properties of the hydrogel. The results revealed that at 64.83 ℃, the elastic modulus of the hydrogel reached 277.61 Kpa. The cross-linking agent and modifier amounts were determined to be 0.122 and 4.669 g, respectively. Subsequently, the mechanical properties of the kitchen hydrogels were researched by investigating the experimental mode of interaction between the experiment and molecular dynamics simulation. The simulation results were consistent with the experimental data, acting as a reference for the in-depth study of other properties of the kitchen hydrogel system. Graphical
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MEDICINAL CHEMISTRY RESEARCH
·
2024
·
IF 2.6
Cheng Wenhui,Zhang Yuejuan,Chen Cheng,Gao Lei,Lv Yuwei,Yu Dian,Chen Bin,Wan Yi
The production of carbapenemases is a significant mechanism contributing to carbapenem resistance in Enterobacteriaceae . Among these carbapenemases, OXA-48 presents a distinct challenge and threat, as most β-lactamase inhibitors do not effectively inhibit its activity. In this study, we investigated the inhibitory potential of α-mangostin against OXA-48 with an IC 50 value of 0.52 μM. Enzyme activity assays demonstrated that α-mangostin inhibited OXA-48 reversibly through a non-competitive and dose-dependent inhibition mode. The docking analysis revealed that the 7-hydroxyl group of α-mangostin formed hydrogen bonds with Thr197 and Trp222, whereas the 5-hydroxyl group and the 4-carbonyl group interacted with Lys116 and Met115. Our study indicates that α-mangostin exhibits significant inhibition against OXA-48 and holds promise as a potential compound for the development of β-lactamase inhibitors.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2024
·
IF 3.5
Liu Jiale,Zhao Shufan,Wei Wan,Yu Shupeng,Wang Zhao,Zheng Jianyong
Lipase is one of the most widely studied and applied biocatalysts. Due to the high enzyme leakage rate of the immobilization method of physical adsorption, we propose a new lipase immobilization method, based on the combination of macroporous resin adsorption and organic polymer coating. The immobilized Candida antarctica lipase B (CALB@resin-CAB) was prepared by combining the macroporous resin adsorption with cellulose acetate butyrate coating, and its structure was characterized by various analytic methods. Immobilized lipase was applied for biodiesel production using acidified palm oil as the starting material, the conversion rate achieved as high as 98.5% in two steps. Furthermore, the immobilized lipase displayed satisfactory stability and reusability in biodiesel production. When the aforementioned reaction was carried out in a continuous flow packed bed system, the yield of biodiesel was 94.8% and space–time yield was 2.88 g/(mL∙h). The immobilized lipase CALB@resin-CAB showed high catalytic activity and stability, which has good potential for industrial application in the field of oil processing.
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METALLURGICAL AND MATERIALS TRANSACTIONS A-PHYSICAL METALLURGY AND MATERIALS SCIENCE
·
2024
·
IF 2.2
Tang Qin,Li Xiaoping,Liu Guangyin,Zhai Dajun,Shen Jun
Magnesium (Mg) and its alloys face challenges in clinical applications due to their high degradation rates and low antibacterial properties. To overcome these challenges, this study employed an electrolyte containing elements such as F, Ca, P, Si, Sr, and Ag to perform soft spark micro-arc oxidation (MAO) on ZrO 2 –AZ91D Mg alloy. The research encompassed analysis of the MAO process, characterization of the composite coating's phase composition and elemental valence states, measurement of the physicochemical properties of the coating, as well as evaluation of biological properties including cell adhesion, protein adsorption, cytotoxicity, and antibacterial activity. This study investigated the mechanisms underlying osteogenesis and antibacterial effects of co-doped coatings, and examined the influence of bioactive elements on the performance of MAO coatings. The results demonstrated that the co-doped F/Ca/Sr/Ag particle-containing MAO composite coating exhibited excellent wettability and corrosion resistance. Additionally, the coating displayed remarkable protein adsorption capacity, superior adhesion to MC3T3-E1 osteoblast cells, and excellent antibacterial efficacy against Staphylococcus aureus, all while showing negligible toxicity.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-20
·
IF 10.6
Yao Xueting,Gong Zehua,Yin Wenyan,Li Hanbing,Douroumis Dennis,Huang Lijiang,Li Huaqiong
The primary issues in treating type 1 diabetes mellitus (T1DM) through the transplantation of healthy islets or islet β-cells are graft rejection and a lack of available donors. Currently, the majority of approaches use cell encapsulation technology and transplant replacement cells that can release insulin to address transplant rejection and donor shortages. However, existing encapsulation materials merely serve as carriers for islet cell growth. A new treatment approach for T1DM could be developed by creating a smart responsive material that encourages the formation of islet cell spheroids to replicate their 3D connections in vivo and controls the release of insulin aggregates. In this study, we used microfluidics to create thermally sensitive porous scaffolds made of poly(N-isopropyl acrylamide)/graphene oxide ( P NIPAM/GO). The material was carefully shrunk under near-infrared light, enriched with mouse insulinoma pancreatic β cells (β-TC-6 cells), encapsulated, and cultivated to form 3D cell spheroids. The controlled contraction of the thermally responsive porous scaffold regulated insulin release from the spheroids, demonstrated using the glucose-stimulated insulin release assay (GSIS), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assay. Eventually, implantation of the spheroids into C57BL/6 N diabetic mice enhanced the therapeutic effect, potentially offering a novel approach to the management of T1DM. Graphical Abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 27
·1-12
·
IF 2.8
Zeng Shifan,Zhang Cong,Tian Haiming,Yao Guizhi,Shi Huan,Zhu Yong,Chi Shangpeng,Kan Xucai,Liu Xiansong
The magnetic powder Sm 2 Fe 17 N x has recently garnered considerable interest due to its competitive pricing. In this study, the preparation of Sm 2 Fe 17 N x magnetic powder by melt quenching and nitriding process was successfully completed. In order to obtain a higher coercivity, the particle size of the magnetic powder was reduced to a diameter of less than 2 μm through ball milling. It was observed that as the ball milling time was extended, the coercivity of the magnetic powder exhibited a decline that was premature in comparison to the expected results. Additionally, the presence of minute particles of magnetic powder may appear spontaneous combustion phenomenon. Accordingly, Sm 2 Fe 17 N x magnetic powder with high coercivity ( H cj = 842.8 kA/m) was prepared by phosphoric acid passivation process. Through XRD patterns, it was found that the phosphoric acid passivation process markedly enhanced the antioxidant capacity of the magnetic powder. Through SEM images, it is found that the fine magnetic powder exhibits a sheet structure and has strong shape anisotropy. The research indicate that the phosphoric acid passivation process is effective in enhancing the stability of Sm 2 Fe 17 N x magnetic powder and inhibiting the oxidation reaction of magnetic powder during use. This has a beneficial impact on the service life of permanent magnet devices in industrial production.
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