Journal of Translational Medicine
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2024
·Vol. 22
·期 1
·1-17
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IF 6.1
Gong Sisi,Huang Rongfu,Wang Meie,Lian Fen,Wang Qingshui,Liao Zhijun,Fan Chunmei
Triple-negative breast cancer (TNBC) is known for its aggressive nature, lack of effective diagnostic tools and treatments, and generally poor prognosis. The objective of this study was to investigate metabolic changes in TNBC using metabolomics approaches and explore the underlying mechanisms through integrated analysis with transcriptomics. In this study, serum untargeted metabolic profiles were first examined between 18 TNBC patients and 21 healthy control (HC) subjects using liquid chromatography-mass spectrometry (LC-MS), identifying a total of 22 significantly differential metabolites (DMs). Subsequently, receiver operating characteristic analysis revealed that 7-methylguanine could serve as a potential biomarker for TNBC in both the discovery and validation sets. Additionally, transcriptomic datasets were retrieved from the GEO database to identify differentially expressed genes (DEGs) between TNBC and normal tissues. An integrative analysis of the DMs and DEGs was conducted, uncovering potential molecular mechanisms underlying TNBC. Notably, three pathways—tyrosine metabolism, phenylalanine metabolism, and glycolysis/gluconeogenesis—were enriched, providing insight into the energy metabolism disorders in TNBC. Within these pathways, two DMs (4-hydroxyphenylacetaldehyde and oxaloacetic acid) and six DEGs (MAOA, ADH1B, ADH1C, AOC3, TAT, and PCK1) were identified as key components. In summary, this study highlights metabolic biomarkers that could potentially be used for the diagnosis and screening of TNBC. The comprehensive analysis of metabolomics and transcriptomics data offers a validated and in-depth understanding of TNBC metabolism.
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Scientific Reports
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2024
·Vol. 14
·期 1
·1-15
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IF 3.8
Wu Ying,Wu Zhiyi,Gao Yinyin,Fan Yixuan,Dong Yao,Zhang Yinan,Gai Zhonghui,Gu Shaobin
This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, and cell adhesion and tolerance studies, along with acute and subacute oral toxicity studies in animal models. BC99 was isolated from a well-characterized collection originating from the feces of a healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates and broad antibiotic sensitivity, including to gentamicin, ampicillin, chloramphenicol, ciprofloxacin, and others. Cytotoxicity testing confirmed no adverse effects on HT-29 cells and significant adhesive properties to intestinal epithelial cells. Tolerance tests demonstrated over 90% viability of BC99 under simulated gastrointestinal conditions. In vivo studies in mice and rats confirmed the absence of adverse effects following oral administration. Collectively, these findings support BC99’s robust tolerance to gastrointestinal environments, strong adhesion capabilities, and a broad spectrum of antibiotic resistance, underlining its potential as a safe and effective agent for gut microbiota modulation and host health enhancement.
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Journal of Inorganic and Organometallic Polymers and Materials
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2024
·
IF 3.9
Yang Jie,Yan Zhuo,Dai Jiangdong
With the rapid industrial development worldwide, the volume of oily wastewater, including petroleum spills and industrial effluents, has increased dramatically, posing a threat to ecosystems and human health. Membrane separation technology, known for its advantages of phase-transition-free operation, low energy consumption, and simplicity, has gained rapid popularity in wastewater treatment. This study focused on modifying PVDF membranes using PVA-TA hydrogels with two distinct coordination strategies: TA-Fe 3+ coordination and APTMS covalent cross-linking. The resulting composite membranes exhibited superhydrophilic/underwater superoleophobic properties with high emulsion flux (3800–6900 and 4200–12,000 Lm − 2 h − 1 bar − 1 ) and separation efficiency (> 98.9% and > 99.3%) for various oil-water emulsions. Notably, the stability of the hydrogel network allowed the composite membranes to maintain elevated fluxes (6847.2 and 10266.7 Lm − 2 h − 1 bar − 1 ) and separation efficiencies (97.8% and 99.3%) even after 10 cycles for dichloroethylene-water emulsion. The addition of Fe 3+ and APTMS enhances hydrogel stability and roughness, with APTMS contributing to superior wetting and separation performance. This work provides valuable insights into the impact of different coordination mechanisms on hydrogel-coated membranes, offering a foundation for the development of advanced materials in efficient oil-water separation.
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Nature Protocols
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2024
·
IF 13.1
Wang Aoxue,Zou Yejun,Liu Shuning,Zhang Xiuze,Li Ting,Zhang Lijuan,Wang Ruwen,Xia Yale,Li Xie,Zhang Zhuo,Liu Tiemin,Ju Zhenyu,Wang Ru,Loscalzo Joseph,Yang Yi,Zhao Yuzheng
As a key glycolytic metabolite, lactate has a central role in diverse physiological and pathological processes. However, comprehensive multiscale analysis of lactate metabolic dynamics in vitro and in vivo has remained an unsolved problem until now owing to the lack of a high-performance tool. We recently developed a series of genetically encoded fluorescent sensors for lactate, named FiLa, which illuminate lactate metabolism in cells, subcellular organelles, animals, and human serum and urine. In this protocol, we first describe the FiLa sensor-based strategies for real-time subcellular bioenergetic flux analysis by profiling the lactate metabolic response to different nutritional and pharmacological conditions, which provides a systematic-level view of cellular metabolic function at the subcellular scale for the first time. We also report detailed procedures for imaging lactate dynamics in live mice through a cell microcapsule system or recombinant adeno-associated virus and for the rapid and simple assay of lactate in human body fluids. This comprehensive multiscale metabolic analysis strategy may also be applied to other metabolite biosensors using various analytic platforms, further expanding its usability. The protocol is suited for users with expertise in biochemistry, molecular biology and cell biology. Typically, the preparation of FiLa-expressing cells or mice takes 2 days to 4 weeks, and live-cell and in vivo imaging can be performed within 1–2 hours. For the FiLa-based assay of body fluids, the whole measuring procedure generally takes ~1 min for one sample in a manual assay or ~3 min for 96 samples in an automatic microplate assay.
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FIBERS AND POLYMERS
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2024
·
IF 2.2
He Xia,Liu Qingchun,Pan Lianjun,Zhou Ying,Xu Le,Zhou Shiyu,Ma Yixin
Wearable electronics based on natural biocompatible fibers have attracted considerable interests due to the promising use for healthcare monitoring, human–machine interactions, and smart clothing. However, crucial challenges to design robust, flexible, and highly sensitive fiber sensors remain, to meet various requirements for practical application. Herein, Fischer esterification and in situ polymerization technologies were employed to produce conductive nanocelluloses (CNC–PEDOT), which possessed excellent mechanical stiffness and high conductivity (238 μS/cm). Then, silk yarns as supporting materials were functionalized by as-prepared conductive units to construct all-in-one fiber sensor (SCP) for multiple signals monitoring. Surprisingly, the resulted SCP fiber showed impressive mechanical performances (422.86 MPa at 18.44%), due to the strong hydrogen-bond interaction between SY substrate and CNC–PEDOT conductive units. More importantly, SCP fiber was employed a dual-function sensor for real-time monitoring of strain and temperature, illustrating remarkable sensitivities, i.e., gauge factor = 4.28 in a large strain range of 0–16%, and sensitivity = 1.55%/℃ in a broad temperature range of 25–60 ℃. The SCP fiber sensor with impressive mechanical performances and high sensitivity, can be applied for real-time health monitoring in wearable biosensors, smart healthcare, and on-demand therapy.
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RARE METALS
·
2025
·
IF 11
Wang Ding,Qin Rui-Jie,Yu Jie-Jie,Hu Jin-Wu,Zhang Wen-Hui,Xu Hui,Xu Jing-Cheng,Liao Qiao-Bo,Li Hui-Jun,Wang Xu-Hui
The semiconductor gas sensors used for xylene gas detection in real time has been restricted by the inadequate sensitivity and selectivity. Constructing a bilayer cascade sensor with the catalysis-gas sensitivity synergistic is considered as an effective solution. Herein, the Ag@CeO 2 nanosheets are synthesized by heat treating the Ag@Ce-MOF, which synthesized via solvothermal method. The morphological evolution of cerium metal–organic framework (Ce-MOF), regulated by Ag ions, is investigated, and the transformation mechanism is proposed. The bilayer sensors were constructed by using WO 3 nanofibers, prepared via the electrospinning method, as the sensitive layer and the Ag@CeO 2 nanosheets as the catalytic layer, respectively. The bilayer sensors exhibit remarkable performance in response to xylene. The response value ( R a / R g ) of WO 3 /Ag@CeO 2 sensor to 10 ppm xylene gas reaches 32.13 at the operating temperature of 160 °C. Additionally, the sensor displays an exceptional response to even trace amounts of xylene, as low as parts per billion (ppb). The catalysis-gas sensitivity synergistic mechanism was elucidated by capturing catalytic intermediates using online mass spectrometry. These findings provide a novel strategy for benzene series (BTEX) sensor and offer a novel approach to prepare two-dimensional Ce-MOF and its derived materials with tailored properties. Graphical abstract
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-9
·
IF 5.3
Zhang Qijia,Wang Yuting,Ma Dandan,Pan Xing,Sheng Enze,Shen Jiachen,Liang Dongbing,Wang Chao,Qian Chen,Qian Wenhui,Zhu Dong
A highly sensitive dual-recognition fluorescence amplification method is presented for lipopolysaccharide (LPS) detection based on boronic functionalized aptamer macroarrays with dual-recognition and isothermal amplification. The surface of the polystyrene microplate was firstly carboxylated, and then, 3-aminophenylboronic acid was conjugated to the carboxyl groups through EDC/NHS reaction, creating boronic acid groups as the capture moiety for LPS. A recognition DNA aptamer labeled with the fluorescent dye 6-FAM, which exhibits specificity towards LPS, was selected as the signal reporting moiety. By introducing primers and Klenow enzyme, the fluorescent-labeled aptamers are released from the microplate bottom, and double-stranded structures were formed via isothermal amplification. The addition of SYBR Green I, which strongly fluoresces upon binding to the double-stranded structures, enables signal amplification and detection. This detection method exhibits a linear range of 1–10,000 ng/mL and has a detection limit as low as 401.93 pg/mL. This analytical approach shows high selectivity and sensitivity and may serve as a universal platform in lipopolysaccharide detection.
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Microbiome
·
2024
·Vol. 12
·期 1
·1-24
·
IF 13.8
Lyu Zhengtian,Yuan Gaoxiang,Zhang Yuying,Zhang Fengwenhui,Liu Yan,Li Yifan,Li Guang,Wang Ying,Zhang Ming,Hu Yongfei,Guo Yuming,Liu Dan
Background The gut microbiota is a key regulator of bone metabolism. Investigating the relationship between the gut microbiota and bone remodeling has revealed new avenues for the treatment of bone-related disorders. Despite significant progress in understanding gut microbiota-bone interactions in mammals, research on avian species remains limited. Birds have unique bone anatomy and physiology to support egg-laying. However, whether and how the gut microbiota affects bone physiology in birds is still unknown. In this study, we utilized laying hens as a research model to analyze bone development patterns, elucidate the relationships between bone and the gut microbiota, and mine probiotics with osteomodulatory effects. Results Aging led to a continuous increase in bone mineral density in the femur of laying hens. The continuous deposition of medullary bone in the bone marrow cavity of aged laying hens led to significant trabecular bone loss and weakened bone metabolism. The cecal microbial composition significantly shifted before and after sexual maturity, with some genera within the class Clostridia potentially linked to postnatal bone development in laying hens. Four bacterial strains associated with bone development, namely Blautia coccoides CML164, Fournierella sp002159185 CML151, Anaerostipes caccae CML199 (ANA), and Romboutsia lituseburensis CML137, were identified and assessed in chicks with low bacterial loads and chicken primary osteoblasts. Among these, ANA demonstrated the most significant promotion of bone formation both in vivo and in vitro , primarily attributed to butyrate in its fermentation products. A long-term feeding experiment of up to 72 weeks confirmed that ANA enhanced bone development during sexual maturity by improving the immune microenvironment of the bone marrow in laying hens. Dietary supplementation of ANA for 50 weeks prevented excessive medullary bone deposition and mitigated aging-induced trabecular bone loss. Conclusions These findings highlight the beneficial effects of ANA on bone physiology, offering new perspectives for microbial-based interventions for bone-related disorders in both poultry and possibly extending to human health.
Video Abstract Graphical Abstract
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Chinese Medicine
·
2025
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IF 5.7
Ruiyu Wu,Runping Liu,Ranyun Chen,Yijie Li,Xiaoyong Xue,Yinhao Zhang,Fanghong Li,Jiaorong Qu,Lingling Qin,Chen Wang,Xiaojiaoyang Li
Background Obesity is frequently linked to chronic systamic inflammation and presents significant challenges to public health. Aurantio-obtusin (AO) boosted the brown adipose tissue (BAT) thermogenesis in diet-induced obesity. However, the specific mechanisms by which injured mitochondria-related damage signals derived from overwhelmed BAT can transmit to liver and exacerbate metabolic disorders and whether AO can reverse this process remain unknown. Materials and methods After applying high-fat diet and glucose-fructose water (HFHS)-induced obesity mice, different BAT transplant procedures and primary BAT adipocytes, we investigated the anti-obesity effects and mechanism of AO through RNA sequencing and biology techniques. Results AO improved whole-body lipid accumulation, mitochondrial metabolism in BAT and hepatic inflammation in HFHS-induced obesity mice. Interscapular transplant of BAT-derived from obese donor mice triggered hepatic inflammation of chow diet-fed recipient mice, which was protected by AO. Furthermore, the transplantation of BAT-derived from AO-treated mice protected hepatic inflammation in obese mice. In vivo and in lipid-challenged primary BAT adipocytes, AO decreased kexin type 9 (PCSK9), prevented mPTP opening and mitochondrial DNA (mtDNA) release in extracellular vesicles (EVs) manner by inhibiting the acetylation of cyclophilin D associated with adenine nucleotide translocase, suppressing oligomerization of voltage-dependent anion channel 1 and activating mitophagy. Ultimately, AO inhibited mtDNA-containing EVs-induced cyclic GMP-AMP synthase/stimulator of interferon genes (STING) activation and hepatic inflammation, which was confirmed by Sting −/− mice. Conclusion AO not only improves thermogenesis and mitochondrial function of BAT but also prevents liver inflammation by repairing mitochondrial function and blocking the transfer of mtDNA from BAT to the liver. Graphic abstract Supplementary Information The online version contains supplementary material available at 10.1186/s13020-025-01097-y.
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Biochar
·
2024
·Vol. 6
·期 1
·1-14
·
IF 13.1
Chen Shanshuai,Tian Liu,Feng Xiaoqiang,Bao Huanyu,Wang Hongliang
Chitin and phytic acid are abundant sustainable resources commonly found in shrimp shells, crab shells, and various plants. However, they are underutilized, and their biomass value is frequently underestimated. To address this, the current study developed a strategy to synthesize efficient separator coatings for zinc-iodine (Zn-I) batteries using chitin and phytic acid as carbon sources (biochar). First, nanosheets of porous carbon doped with N and P (NP-PC), featuring multiple active sites, are synthesized through in situ carbonization of chitin and phytic acid. Subsequently, the synthesized materials are coated onto the surfaces of glass fiber (GF) separators, effectively addressing several persistent challenges associated with Zn-I batteries. Notably, the modified separator (NP-PC@GF) enhanced the rapid-discharge capability of the involved battery, effectively inhibited the diffusion of intermediate polyiodide species, stabilized the electrolyte interface, suppressed irreversible dendrite formation, and increased I utilization efficiency. Consequently, the battery demonstrated high electrochemical performance, with an initial capacity of 7.8 mAh cm –2 at 20 mA cm –2 , an initial specific capacity of 8.9 mAh cm –2 under high I loading, and a retention rate of 56% (5.1 mAh cm –2 ) at the 174th cycle. Overall, this research demonstrates the effective use of biochar for separator modification, facilitating the development of high-performance Zn-I batteries. Graphical
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Nano Research
·
2024
·
IF 9.5
Tang Jianfu,Fan Xueqin,Huang Haozhou,Dong Xiaofei,Li Xueqi,Wang Peiru,Yin Ran,Xie Yanjun,Li Jian,Tan Gang,Pang Zhenqian,Gan Wentao
Using biodegradable material derived from renewable resources as petroleum-based plastics replacement is a promising way towards sustainable development. However, the insufficient mechanical properties and complex manufacturing process of bioplastics still need to be improved for high-quality food packages. Herein, we report a top-down strategy to transform natural wood into a clear wood packaging film through scalable delignification and polyvinyl alcohol (PVA) infiltration. The wood packaging film demonstrates a laminated structure with completely collapsed cell walls and PVA intertwined together after energy-saving air drying, resulting in high light transmittance with low haze, good mechanical performance, and high barrier performance for oxygen and water vapor. Molecular dynamics simulations reveal the underlying fracture mechanism between cellulose and PVA, which effectively enhances the Young’s modulus and strength of the wood packaging film. These findings contribute to the development of biodegradable and strong packaging materials, as well as other food-related applications, using sustainable wood.
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Nano Research
·
2024
·
IF 9.5
Wang Yong,Guo Wenting,Wang Bo,Zhou Ya,Hu Ping,Ren Jiangtao,Wang Erkang,Jin Yongdong
Nanofluidic devices have turned out to be exemplary systems for investigating fluidic transport properties in a highly restricted area, where the electrostatic interactions or chemical reactions between nanochannel and flowing species strongly dominate the ions and flow transport. Numerous nanofluidic devices have recently been explored to manipulate ion currents and construct electronic devices. Enlightened by electronic field effect transistors, utilizing the electric field effect of nanopore nanochannels has also been adopted to develop versatile nanofluidic devices. Here, we report a nanopore-based nanofluidic unijunction transistor composed of a conical glass nanopipette with the biomaterial polydopamine (PDA) coated at its outer surface. The asfabricated nanofluidic device exhibited negative differential resistance (NDR) and ion current oscillation (ICO) in ionic transport. The pre-doped copper ions in the PDA moved toward the tip as increasing the potential, having a robust shielding effect on the charge of the tip, thus affecting the surface charge density of the nanopore in the working zone. Finite element simulation based on a continuum model coupled with Stokes-Brinkman and Poisson-Nernst-Planck (PNP) equations revealed that the fluctuations in charge density remarkably affect the transport of ionic current in the nanofluidic device. The as-prepared nanofluidic semiconductor device was a ready-to-use equipment that required no additional external conditions. Our work provides a versatile and convenient way to construct nanofluidic electronic components; we believe by taking advantage of advanced surface modification methods, the oscillation frequency of the unijunction transistors could be controlled on demand, and more nanofluidic devices with resourceful functions would be exploited.
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