PLoS Neglected Tropical Diseases
·
2025
·Vol. 19
·期 1
·e0012769
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IF 3.4
Wenmin Qi,Fei Shen,Chuyue Wang,Juan Wen,Xi Pan,Zhongying Zhao,Lihua Xiao,Yaoyu Feng,Dongjuan Yuan
Fatty acid and retinol binding proteins (FARs) are lipid-binding protein that may be associated with modulating nematode pathogenicity to their hosts. However, the functional mechanism of FARs remains elusive. We attempt to study the function of a certain FAR that may be important in the development of Nippostrongylus brasiliensis . Nb -FAR-1 was highly expressed throughout developmental stages by RNA-seq data and qPCR analyses, and Nb -FAR-1 was a secretory protein and abundant in the excretory-secretory products. Nb -FAR-1 could bind fatty acids and retinol. Fatty acid pattern of parasitic adults was more similar to rat intestine than to free-living L3s, indicating that N . brasiliensis may be dependent on the host to obtain fatty acids. Lentivirus-mediated RNAi was performed on L3s, resulting in a reduction in the expression of Nb - far-1 gene. Furthermore, these RNAi effects could be maintained in several generations. The offspring L3s in Nb - far-1 RNAi group had a reduction in lipid droplets within the subcuticle and the swelling of the perioral epidermis, accompanied with down-regulated expression of enzymes in amino acid and glycerolipid metabolism and glycometabolism for growth by RNA-seq data. Adults in Nb - far-1 RNAi group had the crumpled epidermis loosely attached to the basal membrane of body surface and the breakage of mouth epidermis, accompanied with a decrease in adult egg-shedding and an appearance of abnormal eggs. In vitro culture of eggs showed decreased efficiency of egg hatchability and larval development in the Nb - far-1 RNAi group. Transcriptomic analysis showed that interference with Nb - far-1 expression induced downregulated expression of major sperm protein and serpin for reproduction, and collagen for epidermis formation in adults, most of which were relatively high expression in adults but low expression in L3s in the WT group. Thus, Nb -FAR-1 may affect the reproduction, growth, and development of N . brasiliensis by regulating the level of lipids.
Fatty acid and retinol-binding protein 1 (FAR-1) is lipid-binding protein that may be associated with modulation of host immune responses and provide insights into nematode pathogenicity to their hosts. However, the role of FAR-1 protein on parasitic nematodes remains unclear. Nippostrongylus brasiliensis was employed to identify the characteristics of Nb - far-1 gene, its expression patterns, ligand binding properties and the functions associated with it. The secretory Nb -FAR-1 protein was found to be highly expressed in N . brasiliensis ESPs and demonstrated affinity for fatty acids and retinol. Interference with Nb - far-1 gene expression resulted in a reduction in the formation of lipid droplets in L3s, a decrease in a rate of adult egg-shedding, egg hatchability, and larval development. Therefore, we proposed that N . brasiliensis Nb -FAR-1 protein may serve as a causative agent in nematode growth, development, and reproduction by regulating the level of lipids.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Xiaolin Ma,Xuan Zhao,Yulong Yang,Jinjin Yan,Xiaoyan Shi,Hongfei Wu,Yarong Liu,Min Dai
Atherosclerosis is a chronic inflammatory disease characterized by lipid streaks, which are produced by aggregates of lipid-rich foam cells. Foam cells intensify atherosclerosis by secreting a range of inflammatory mediators. Neutrophil extracellular traps produced by activated neutrophils, which are abundantly present in lipid-accumulating plaques. However, the relationship between neutrophil extracellular traps and foam cells inflammation is still unclear. Paeonol is well known for its anti-inflammatory effects in atherosclerosis. Nevertheless, the exact pharmacological mechanisms by which paeonol affects atherosclerosis are not fully understood which require further investigation. The purpose of this study is to investigate the effects of paeonol on the neutrophil extracellular traps' formation and foam cell inflammation caused by neutrophil extracellular traps, and to explore the potential mechanisms. A high-fat diet was administered to ApoE −/− mice for a period of 12 weeks to induce an atherosclerosis model. Our findings demonstrated that paeonol notably suppressed the advancement of atherosclerosis in ApoE −/− mice, curtailed the formation of neutrophil extracellular traps, and lowered inflammatory factor levels within the plaque. In vitro studies have shown that neutrophil extracellular traps could enhance the inflammation in foam cells. CitH3 played a role in the cellular communication between neutrophil extracellular traps and foam cells. Concurrently, NLRP3 acted as a key receptor in the inflammation mediated by this interaction. Paeonol is capable of regulating NE, thereby affecting the formation of neutrophil extracellular traps. Most notably, the foam cell inflammation caused by neutrophil extracellular traps was significantly mitigated by the inclusion of paeonol. Our findings suggested that paeonol inhibited foam cell inflammation which induced by neutrophil extracellular traps through the CitH3/NLRP3/caspase-1 signaling pathway, shedding new lights on its anti-atherosclerotic pharmacological mechanism.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2018
·
IF 2.196
Xiangyu Zhang,Neng Gao,Yongping Wu,Guangming Chen
To improve the performance of an absorption system, ionic liquids (ILs) have been chosen as additives to conventional working fluid (lithium bromide aqueous solution) in recent years. In this paper, 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) was added to lithium bromide aqueous solution (mass ratio LiBr/[Emim]Ac = 3) to measure the vapor–liquid equilibrium (VLE) data of the ternary system. The experiment was conducted by means of boiling point method, at temperatures between 303.94 K and 405.41 K and mass fractions of absorbent between 0.20 and 0.60. The experimental data were regressed using an Antoine-type equation, and the average absolute relative deviation (AARD%) between the experimental and calculated values was 0.34%, which showed excellent accuracy and consistency. The experimental ternary system was compared with the conventional LiBr + H2O system and two other ternary systems from the literature; the result revealed that the experimental ternary system had lower vapor pressures, which will benefit the process of releasing latent heat in absorption cycles.
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MOLECULES
·
2024
·Vol. 29
·期 13
·3046
·
IF 4.2
Jingyi Zhang,Congmei Lin,Feng Li,Xinhao Wei,Yusen Chen,Yanyong Fu,Xiaoping Yu,Biao Zhang,Zihong Ye
Cancer is one of the major public health challenges in the world, which is characterized by rapid progression and high mortality. Immunotherapy, represented by PD-1 monoclonal antibody, has significantly improved the efficacy of malignant tumors and has become one of the most popular immunotherapy methods at present. Therefore, there is an increasing demand for novel detection methods for PD-1 monoclonal antibodies. The aim of this work was to establish a rapid, simple, and sensitive immunochromatographic test strip (ICTS) based on the AuNPs enlargement for both visual and instrumental detection of the PD-1 monoclonal antibody concentration. The mixed solution of NH2OH·HCl and HAuCl4was used as an enhancement solution to lower the detection limit and achieve higher sensitivity. A test strip reader was used to construct a visualized quantitative detection standard curve for the PD-1 monoclonal antibody concentration. The LOD was 1.58 ng/mL through a triple signal-to-noise ratio. The detection time was within 10 min. The constructed test strips can rapidly, accurately, and efficiently detect the concentration of PD-1 monoclonal antibody in real samples.
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BIORESOURCE TECHNOLOGY
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2012
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IF 4.98
Yongshui Qu,Chongpin Huang,Yanlei Song,Jie Zhang,Biaohua Chen
The dehydration of fructose or glucose to 5-hydroxymethylfurfural (5-HMF) using room temperature ionic liquids (ILs) as a solvent is a promising method for producing liquid fuels from renewable resources . The IL, 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ([C 2 OHMIM]BF 4 ), was used a catalyst—rather than as a solvent—in the conversion of fructose or glucose to 5-HMF. With glucose, the yield of 5-HMF reached as high as 67.3% after 1 h at 180 °C in dimethylsulfoxide (DMSO) as solvent. The catalyst was separated from the reaction mixture by distilling solvent and reused six times without loss of activity. Furthermore, a kinetic analysis was carried out to illustrate the formation of 5-HMF, and the values of the activation energy and the pre-exponential factor for the reaction were 55.77 kJ mol –1 and 1.6 × 10 4 min –1 respectively.
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JOURNAL OF MEMBRANE SCIENCE
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2025
·
IF 9
Hongjin Li,Yongchao Sun,Jianyu Guan,Lu Bai,Tianyou Li,Fake Sun,Yijun Liu,Gaohong He,Canghai Ma
Mixed matrix membranes (MMMs), composed of polymers and fillers, capitalize on the complementary strengths of both materials, offering a promising approach for developing high-performance membranes for C 3 H 6 /C 3 H 8 separation. However, the effectiveness of MMMs is often hindered by poor interfacial compatibility between the polymer and the filler. To address this challenge, we present an effective strategy that simultaneously enhances both interfacial compatibility and gas separation performance in MMMs. By etching ZIF-8 with cyanuric acid (CA), we successfully synthesized defect-engineered ZIF-8 (DZIF-8), a hollow nanoframe structure that features open metal sites. These sites not only coordinate with the amidoxime groups of amidoxime-functionalized PIM-1 (AO-PIM-1) to improve interfacial compatibility but also significantly enhance the adsorption capacity for propylene. The resulting MMMs exhibited excellent interfacial compatibility with no observable filler agglomeration. Remarkably, the 10 % DZIF-8-based MMM enhances C 3 H 6 permeability to 492 and boosts C 3 H 6 /C 3 H 8 selectivity to 24. The defect-engineered MOF-based MMM (DMMM) surpassed the 2003 upper bound for C 3 H 6 /C 3 H 8 separation and demonstrated outstanding resistance to plasticization. Furthermore, the DMMM exceeded the 2008 Robeson upper bound for H 2 /CH 4 (N 2 ) separation performance, highlighting its exceptional versatility. These results showcase the potential of defect-engineered MMMs in gas separation applications, particularly in high-performance C 3 H 6 /C 3 H 8 separation.
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Shaojie Ma,Yuchen Zhu,Boxin Chen,Bin Zhao,Fei Feng
Background The micro gas chromatography column (μGCC) is one of the key components of the miniaturized gas chromatography system. However, light alkanes are difficult to be separated by a micro gas chromatography column , especially for methane and ethane, because the length of μGCC is limited by the area of a silicon substrate. More importantly, the heterogeneous microchannel surface formed by silicon glass bonding causes uneven stationary phase coating and the forces between the untreated microchannel surfaces and the stationary phase materials are weak, which will prevent the improvement of separation performance. Results In this paper, a micro gas chromatography column (μGCC) with uniform HKUST-1 stationary phase is reported. Significantly, an alumina film prepared by the atomic layer deposition (ALD) technique is used to homogenize the heterogeneous microchannels. The alumina is a hydrophilic material and the alumina made by the ALD technique is uniform. The forces between hydrophilic alumina film and HKUST-1 are strong, which can greatly improve the coating uniformity of the hydrophilic stationary phase HKUST-1. The test results show that the μGCC could baseline separate the light alkane mixtures (CH 4 , C 2 H 6 , C 3 H 8 , and C 4 H 10 ) at the high testing temperature of 120 °C. The maximum resolution of the difficult-separated methane and ethane reached 19.2, which is 108 % higher than the μGCC using the same stationary phase without homogenizing the microchannel inner surface. Significance The μGCC uses ALD alumina film to homogenize the microchannel inner surface; meanwhile, hydrophilic ALD alumina has a strong electrostatic attraction with the hydrophilic stationary phase HKUST-1. Homogeneous microchannel surface and strong electrostatic attraction are favorable to obtain uniform stationary phase which greatly improves the separation performance, resulting in a large resolution for methane and ethane. The μGCC has broad application prospects in light alkane separation.
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AMERICAN JOURNAL OF PATHOLOGY
·
2024
·
IF 4.7
Mingxiang Zou,Wenkang Chen,Jing Li,Xin Qi,Xiaobin Wang,Fubing Liu,Jiarui Hu,Qianshi Zhang
This study investigated the role of apoptosis signal-regulated kinase-1 ( ASK1 ) in intervertebral disc degeneration (IDD). The nucleus pulposus (NP) tissues of non-IDD and IDD patients were subjected to hematoxylin and eosin, Safranin O–fast green, and immunohistochemical staining. Quantitative real-time PCR was used to assess the ASK1 mRNA level within NP tissue samples and cells. The Cell Counting Kit-8 assay, senescence-associated β-galactosidase staining, and then flow cytometry were conducted, respectively, to assess the viability, senescence, and apoptosis of NP cells. The extracellular matrix–related factors were detected using Western blot analysis. Furthermore, the effect of ASK1 on the IDD rat model was evaluated through nuclear magnetic resonance imaging analysis, hematoxylin and eosin, Safranin O–fast green staining, and immunohistochemical staining. Finally, c-Jun N-terminal kinase (JNK) inhibitors were used to verify the effect of the JNK/p38 signaling on IDD. ASK1 mRNA and protein were up-regulated within NP tissue samples from the IDD group, IL-1β–stimulated NP cells, and IDD rats. ASK1 inhibition promoted cell viability and repressed the senescence and apoptosis of NP cells; promoted collagen II and aggrecan; inhibited matrix metalloproteinase 3, matrix metalloproteinase 9, a disintegrin and metalloproteinase with thrombospondin motifs 4, and a disintegrin and metalloproteinase with thrombospondin motifs 5 protein levels; and increased NP cells in rat intervertebral disc tissues. ASK1 overexpression exerted the opposite effects of ASK1 inhibition on NP cells. Additionally, JNK/p38 signaling suppression could reverse the ASK1 up-regulation–induced dysfunction. In conclusion, ASK1 facilitated the senescence and apoptosis of NP cells in promoting IDD progression, which may be mediated by the JNK/p38 pathway.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Jianwen Feng,Jiayi Wang,Qiao Gu,Pingting Li,Hongli Xu,Yonghong Deng,Ping Gao
Gel polymer electrolytes (GPEs) hold great promise for lithium (Li) metal batteries (LMBs). Nevertheless, a critical challenge lies in reducing the thickness of GPEs while maintaining their mechanical integrity to achieve high-energy-density LMBs. Additionally, protecting the Li metal anode via electrolyte engineering in GPEs remains demanding. Herein, an innovative ultrathin (1 µm-thick) yet robust GPE developed using an in situ curing technique, featuring a nanofibrous, exceptionally strong polyethylene separator is presented. The unique microstructure, interfacial conformability, and ultrahigh mechanical robustness of the ultrathin polyethylene separator are thoroughly verified. Enhanced ionic association within the GPE is achieved due to the strong affinity of electrolyte solvent with the fluorinated polymer network, as confirmed by large-scale molecular dynamics simulations. The optimized solvation structure with high contact ion pairs and aggregate fractions contributes to forming an anion-derived inorganic-rich solid electrolyte interphase (SEI), thereby protecting the lithium anode. Benefiting from the ultrahigh robustness of GPE and the excellent interfacial stability, the Li metal full cell with a high mass loading LiNi 0.8 Co 0.1 Mn 0.1 O 2 cathode (≈17.3 mg cm −2 ) and thin Li foil anode (50 µm) demonstrates 91% capacity retention after 200 cycles. This design demonstrates a feasible approach toward the practical quasi-solid-state LMBs.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-12
·
IF 3.8
Gao Kexin,Liu Yancui,Sun Cheng,Wang Ying,Bao Hongrong,Liu Guoyang,Ou Jinrui,Sun Ping
Pyroptosis is a gasdermin-mediated pro-inflammatory form of programmed cell death (PCD). Tumor necrosis factor-ɑ (TNF-ɑ) is an inflammatory cytokine, and some studies have shown that TNF-ɑ can cause pyroptosis of cells and exert anti-tumor effects. However, whether TNF-ɑ exerts anti-tumor effects on breast cancer cells by inducing pyroptosis has not been reported. In this study, to explore the impact of TNF-ɑ on pyroptosis in breast cancer cells, we treated MCF-7 cells with TNF-ɑ and found that TNF-ɑ induced cell death. Moreover, we observed that the dead cells were swollen with obvious balloon-like bubbles, which was a typical sign of pyroptosis. Further studies have found that the anti-tumor effect of TNF-ɑ on breast cancer cells in vitro was achieved through the canonical pyroptosis pathway. In addition, TNF-ɑ-induced pyroptosis in MCF-7 cells was associated with mitochondrial dysfunction, in which mitochondrial membrane potential was decreased and mitochondrial ROS production was increased. After inhibiting ROS production, the activation effect of TNF-ɑ on NLRP3/Caspase-1/GSDMD pathway was weakened, and the inhibitory effect of TNF-ɑ on the growth of MCF-7 cells in vitro was also decreased, further confirming the involvement of ROS in TNF-ɑ-induced pyroptosis. Overall, our study revealed a new mechanism by which TNF-ɑ exerts an anti-tumor effect by inducing pyroptosis in MCF-7 cells through the ROS/NLRP3/Caspase-1/GSDMD pathway, which may provide new therapeutic ideas for the treatment of breast cancer.
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Nature Energy
·
2024
·
IF 49.7
Jie Yulin,Wang Shiyang,Weng Suting,Liu Yue,Yang Ming,Tang Chao,Li Xinpeng,Zhang Zhengfeng,Zhang Yuchen,Chen Yawei,Huang Fanyang,Xu Yaolin,Li Wanxia,Guo Youzhang,He Zixu,Ren Xiaodi,Lu Yuhao,Yang Ke,Cao Saichao,Lin He,Cao Ruiguo,Yan Pengfei,Cheng Tao,Wang Xuefeng,Jiao Shuhong,Xu Dongsheng
The development of practical lithium metal cells is plagued by their limited lifespan, primarily due to the poor interfacial stability of the electrolytes. Here we present a compact ion-pair aggregate (CIPA) electrolyte that enables high-performance Li metal pouch cells under lean electrolyte conditions. The electrolyte features a unique nanometre-scale solvation structure in which ion pairs are densely packed to form large CIPAs, in contrast to conventional electrolytes that comprise small aggregates. Notably, the CIPAs facilitate fast interfacial reduction kinetics on the Li metal anode via a collective electron-transfer process, leading to the formation of a stable interface. A 505.9 Wh kg −1 Li metal pouch cell with a high-nickel-content cathode (LiNi 0.905 Co 0.06 Mn 0.035 O 2 ) exhibited a 91% energy retention after 130 cycles. This work demonstrates nanostructured electrolyte design for realizing high-performance Li metal batteries. It also showcases the importance of understanding interfacial reaction mechanisms in the design and development of electrolytes.
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ENERGY & FUELS
·
2024
·
IF 5.2
Guangjie Chen,Guangying Chen,Miao Fa,Yuxian Yang,Yu Wang,Fei Li,Li Sze Lai,Swee Pin Yeap
In order to reduce the regeneration energy consumption of CO2 capture in the postcombustion capture process and explore the regulatory characteristics of phase separation, a series of 1-amino-2-propanol (MIPA)/sulfolane biphasic solutions were proposed to capture CO2. The effects of the molar ratio on the phase separation behavior, CO2 equilibrium absorption, and desorption performances of MIPA/solfolane biphasic absorbents as well as three ternary solutions with the extra addition of ethanolamine (MEA) were investigated comprehensively in experiments. The results showed that the 4M/5M MIPA/sulfolane biphasic solution exhibited excellent performance with higher CO2 loadings in the phase separation point for a CO2-rich liquid (0.4530 mol/mol), better desorption efficiency (85.53%), and relatively low regeneration energy consumption (32% lower than 30 wt % MEA). Besides, MEA has a positive effect on promoting the phase separation of the MIPA/sulfolane solution and reducing the desorption energy consumption. Furthermore, 1H and 13C nuclear magnetic resonance (NMR) spectroscopy was used to explore the reaction and phase separation mechanisms. The results indicated that generated carbamate salts can increase the ionic strength of the aqueous solution, leading to the accumulation of reacted MIPA or MEA in the water phase and the exclusion of sulfolane from water to form a new liquid phase. The results of this work can provide theoretical support for the future industrial application of MIPA/sulfolane biphasic absorbents and offer some guidance for the mechanism exploration of other biphasic solvent systems.
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