Nano Today
·
2026
·
IF 10.7
Jingjiao Li,Mingjia Wu,Jia Dong,Xuanxuan Li,Sujia Liu,Ge Mu,Xuefeng Duan,Jiaqi Lin,Xueguang Lu,Yuhai Bi
Seasonal influenza causes a significant global disease burden every year. Currently licensed injectable influenza vaccines mainly elicit humoral immune responses with minimal mucosal immunity in the respiratory tract, limiting their ability to block viral infection and transmission. Messenger RNA (mRNA) vaccines formulated with lipid nanoparticles (LNPs) offer rapid design and flexibility and are highly immunogenic, yet share this limitation of mucosal immunity. Here, we developed influenza A and B mRNA vaccines (Am80-LNP/IFA and Am80-LNP/IFB) using Am80-loaded LNP (Am80-LNP) encoding viral hemagglutinin (HA). Intramuscular immunization of mice with Am80-LNP/IFB (encoding influenza B/Victoria HA) at 1 and 10 μg doses induced systemic HA-specific IgG responses comparable to those elicited by a commercial LNP formulation (SM102-LNP/IFB) and a licensed split vaccine. Notably, Am80-LNP/IFB markedly enhanced respiratory mucosal immunity, inducing 5.4-fold (1 μg) and 11.8 -fold (10 μg) higher virus-specific IgA in bronchoalveolar lavage fluid relative to SM102-LNP. In addition, Am80-LNP/IFB-10 increased lung CD4⁺ tissue-resident memory T (T RM ) cells by 1.4-fold. Following lethal challenge of the influenza B virus, all mRNA-LNP/IFB vaccinated mice survived without weight loss, whereas the split-vaccinated mice experienced a transient decline to 88% of their initial body weight. Am80-LNP/IFB significantly reduced viral loads, rendering viral RNA undetectable in the lungs and nasal turbinates by 14 days post-infection (dpi) at a 10 μg dose. In addition, the Am80-LNP/IFA vaccine (encoding A/H1N1 HA) achieved complete viral clearance in nasal turbinates by 3 dpi, outperforming the split vaccine. These findings indicate that Am80-LNP enables injectable influenza mRNA vaccines to induce robust systemic immunity together with potent respiratory mucosal IgA and T RM responses, overcoming a major limitation of current inactivated vaccines.
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RSC Advances
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2026
·Vol. 16
·期 33
·30145-30157
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IF 6.1
Mingchen Zhang,Wei Zhao,Yang Zhou,Ruiting Shen,Yiqiong Sun,Lei Jiang,Jiantao Zhang
Diabetic wounds are a serious and increasingly prevalent complication in patients with diabetes, characterized by impaired skin regeneration. A major challenge for effective healing is recurrent bacterial infection, which often resists conventional treatment strategies. In this study, we grafted polymyxin B onto hyaluronic acid and employed Fe3+–EDTA as a cross-linking agent to fabricate HA-B@Fe hydrogels via ionic interactions. Based on the reversible and dynamic coordination between Fe3+ ions and the carboxyl groups of the hyaluronic acid, the resulting hydrogels exhibited shear-thinning behavior and excellent injectability. Based on the combined effects of polymyxin B and Fe3+, the HA-B@Fe hydrogel exhibited potent antimicrobial activity against a broad spectrum of bacteria, achieving >99% bactericidal rates against Escherichia coli (E. coli) and >94% against Staphylococcus aureus (S. aureus). In addition, the HA-B@Fe hydrogel demonstrated great biocompatibility, and showed significant therapeutic efficacy in a diabetic mouse wound model. The mice treated with HA-B@Fe showed a marked reduction in wound size (down to 19%), compared to a residual wound size of 32% in group treated with commercial dressings at day 7. Histopathological analysis further confirmed that HA-B@Fe treatment promoted epidermal regeneration and collagen fiber deposition, suggesting its potential as a promiseing strategy for the clinical treatment of diabetic wounds.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Dan Zu,Jun Yao,Xiao Li,Yixing Huang,Can Hu,Mingcong Deng,Qimei Bao,Yin Shi,Zu Ye,Xiangdong Cheng
Gastric cancer (GC) is one of the most common malignant tumors in the world, which is resistant to conventional chemotherapy drugs. Although polyphyllin B (PB) exhibits antitumor activity against GC, its clinical application is limited by systemic toxicity and poor target specificity. Here, we developed a dual-targeting nanoparticle (Ma/HA@NP) coated with a gastric cancer cell membrane (Ma) and hyaluronic acid (HA) to enhance biocompatibility, immune evasion, and homologous targeting. Ma/HA@NPs preserve the immune evasion and homologous targeting properties of both components. Upon endocytosis, Ma/HA@NPs react with cysteine to release PB, deplete glutathione (GSH), and reduce the enzymatic activity of glutathione peroxidase 4 (GPX4), leading to ferroptosis activation. Compared to single PB, Ma/HA@NPs not only enhance PB release but also trigger immunogenic cell death, promoting dendritic cell maturation and cytotoxic T lymphocyte activation. Moreover, interferon-γ release further suppresses cysteine uptake, synergistically amplifying ferroptosis. In vivo, intravenous administration of Ma/HA@NPs-PB in an orthotopic GC model achieved an 85.4% tumor inhibition rate with reduced systemic toxicity compared to free PB. Together, this dual-targeting platform enhances PB delivery and synergizes ferroptosis with immunogenic cell death (ICD) activation, offering a promising strategy for the treatment of gastric cancer.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiaoya Kou,Shiyi Yang,Jing Zou,Siying Chen,Jianying Wu,Bo Sun,Zhongda Yan,Dezhao Liu,Haoqiang Tan
Recently, increasing attention had been paid for using peracetic acid (PAA) as an efficient disinfectant or a strong oxidant in wastewater treatment. In this study, a rapid and simple spectrophotometric method for the selective determination of PAA in aqueous solution was established, based on the oxidative decolorization of rhodamine B (RhB) by reactive species generated from the combination of PAA and Co(II). Under the optimized conditions (20 μmol L −1 RhB, 1.0 mmol L −1 Co(II), pH = 5.0 buffered with 10 mM acetate acid buffer, and reaction time = 40 s), the extent of RhB decolorization at the characteristic wavelength of 552 nm was found to be proportional to the concentration of PAA in the aqueous solution. A strong linear correlation was observed between the consumption of RhB and the concentration of PAA in the range of 0.25–25 μmol L −1 (R 2 = 0.9991), and the sensitivity for PAA was as high as 2.99 × 10 4 M −1 cm −1 . The RhB spectrophotometric method is effectively tolerant to interference the coexistent H 2 O 2 in PAA solution, the common coexisting species in practical water samples (KCl, MgSO 4 , NaNO 3 , NaHCO 3 , DCF and humic acid) and even the added reducing substances such as hydroxylamine and ascorbic acid. As a selective colorimetric agent, RhB is resistant to interference from reducing substances and water bodies, enabling accurate determination of PAA concentration in actual water environments. Overall, the RhB spectrophotometric method could be used as an alternative method for the cheap, rapid and selective determination of PAA in water samples.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2025
·
IF 6
Jingwen Xu,Yijie Li,Ning Guo,Guangwen Luo,Le Wang,Jiaqi Wang,Jiayi Gao,Wanyang Qin,Lishan Yao,Guoliang Li
Oxidative stress and amyloid- β ( Aβ ) deposition are closely linked to the occurrence and progression of Alzheimer’s disease (AD). Herein, a strategy for alleviating AD by simultaneously inhibiting Aβ aggregation and scavenging excess ROS was proposed. An antioxidant amphiphilic polymer, DSe-Cys-(PEG) 2 , containing diselenide linkages was synthesized to load hydrophilic Schisandrin B (Sch B), which has antioxidant and heat shock response-promoting properties. DSe-Cys-(PEG) 2 self-assembled with Sch B to form nanosized M@Sch B micelles, demonstrating significant scavenging effects on H 2 O 2 , Fe 3+ , ·DPPH, ·ABTS + , and improvement the aging-related phenotypes in Caenorhabditis elegans ( C. elegans ) CL4176, including a 33.33% increase in maximum lifespan, a 51.08% enhancement in locomotion ability, a 38.10% rise in body bending frequency, delayed egg-laying, and paralysis prevention compared to the control group. Furthermore, M@Sch B significantly retarded aging by reducing lipofuscin accumulation and decreasing ROS accumulation by enhancing antioxidases activity and reducing malondialdehyde content. M@Sch B upregulated the expression of anti-aging genes daf-16 , antioxidant stress genes ( skn-1 , sod-3 ), and key heat shock factors ( hsf-1 , hsp-16.2 ), while downregulating aging genes ( daf-2 , age-1 ) and Aβ mRNA expression based on the synergistic antioxidant effect of M and Sch B. M@Sch B can simultaneously mitigates multiple AD-related factors, including synergistic antioxidant effects and inhibition of Aβ aggregation, which are of great significance for delaying aging and treating AD.
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Chinese Journal of Integrative Medicine
·
2025
·
IF 2.5
Liu Shu-yan,Zhang Xiao-wei,Gao Gai,Liu Chang-xin,Chen Hui,Fu Zhong-xue,Xu Jiang-yan,Wang Zhen-zhen,Zhang Zhen-qiang,Xie Zhi-shen
Objective To investigate the therapeutic potential of pseudolaric acid B (PAB) on non-alcoholic fatty liver disease (NAFLD) and its underlying molecular mechanism in vitro and in vivo . Methods Eight-week-old male C57BL/6J mice ( n =32) were fed either a normal chow diet (NCD) or a high-fat diet (HFD) for 8 weeks. The HFD mice were divided into 3 groups according to a simple random method, including HFD, PAB low-dose [10 mg/(kg·d), PAB-L], and PAB high-dose [20 mg/(kg·d), PAB-H] groups. After 8 weeks of treatment, glucose metabolism and insulin resistance were assessed by oral glucose tolerance test (OGTT) and insulin tolerance test (ITT). Biochemical assays were used to measure the serum and cellular levels of total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). White adipose tissue (WAT), brown adipose tissue (BAT) and liver tissue were subjected to hematoxylin and eosin (H&E) staining or Oil Red O staining to observe the alterations in adipose tissue and liver injury. PharmMapper and DisGeNet were used to predict the NAFLD-related PAB targets. Peroxisome proliferator-activated receptor alpha (PPARα) pathway involvement was suggested by Kyoto Encyclopedia of Genes and Genomes (KEGG) and search tool Retrieval of Interacting Genes (STRING) analyses. Luciferase reporter assay, cellular thermal shift assay (CETSA), and drug affinity responsive target stability assay (DARTS) were conducted to confirm direct binding of PAB with PPARα. Molecular dynamics simulations were applied to further validate target engagement. RT-qPCR and Western blot were performed to assess the downstream genes and proteins expression, and validated by PPARα inhibitor MK886. Results PAB significantly reduced serum TC, TG, LDL-C, AST, and ALT levels, and increased HDL-C level in HFD mice ( P <0.01). Target prediction analysis indicated a significant correlation between PAB and PPARα pathway. PAB direct target binding with PPARα was confirmed through luciferase reporter assay, CETSA, and DARTS ( P <0.05 or P <0.01). The target engagement between PAB and PPARα protein was further confirmed by molecular dynamics simulations and the top 3 amino acid residues, LEU321, MET355, and PHE273 showed the most significant changes in mutational energy. Subsequently, PAB upregulated the genes expressions involved in lipid metabolism and mitochondrial biogenesis downstream of PPARα ( P <0.05 or P <0.01). Significantly, the PPARα inhibitor MK886 effectively reversed the lipid-lowering and PPARα activation properties of PAB ( P <0.05 or P <0.01). Conclusion PAB mitigates lipid accumulation, ameliorates liver damage, and improves mitochondrial biogenesis by binding with PPARα, thus presenting a potential candidate for pharmaceutical development in the treatment of NAFLD.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xia Jiang,Ziheng Ding,Wenhua Zhang,Zhicheng Jiang,Bi Shi
Electrocatalytic production of 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF) is a sustainable and clean pathway for harvesting both value-added chemicals and green hydrogen (H 2 ). Herein, B, N doped porous carbon supporting Co (Co/BN-C) with abundant pyridinic N-B species was constructed via a one-pot method using chitosan as precursors, which was further applied for the paired electrocatalysis of an HMF oxidation reaction (HMFOR) and H 2 evolution reaction (HER). H 3 BO 4 as a templating agent could increase the specific surface area of the catalyst and expose more active sites for the HMFOR. Meanwhile, H 3 BO 4 also acted as a B source to dope heteroatoms in the carbon matrix, creating surface defects and thus improving electrical conductivity. Besides, B, N-doped carbon substrate induced the electron deficiency of Co nanoparticle, resulting in the strong adsorption of HMF on the catalyst surface via the interactions between the aldehyde group/furan ring of HMF and the catalyst. Consequently, the Co/BN-C catalyst exhibited remarkable performance with a high HMF conversion (> 99%) and selectivity to FDCA (> 98%) and remained high activity after 8 cycles.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Hamed Veisi,Simzar Hosseinzadeh,Seyed Mahdi Rezayat
Invasive Fungal pulmonary infections caused by opportunistic pathogens pose as significant therapeutic challenges due to the limited efficacy and systemic toxicity of conventional antifungal therapies. This study presents a novel approach for targeted pulmonary drug delivery on Candida albicans and Aspergillus fumigatus as lung infection model. Magnetic quantum dots (Zn0.5 Co0.5 Fe2O4) synthesized by co-precipitation chemical method in hydrothermal condition at rang of quantum dots nearly less than 10 nm. nanoparticles (15 w/w), low molecular weight alginate salt (80 w/w) and amphotericin B (5 w/w); Then suspension was sprayed under a high voltage of 25 kV at a flow rate of 0.4 mL/min. reach a powder in 1–2 µm size is key factor in pulmonary drug delivery. This carriers leverage the dual benefits of controlled drug release and targeting to enhance therapeutic efficacy and reduce systemic side effects. Comprehensive physicochemical characterization revealed the carriers’ spherical morphology, crystalline integrity, and superparamagnetic behavior, as confirmed by TEM, SEM, XRD, and VSM analyses. The carriers demonstrated sustained AmB release over 72 h, as evidenced by UV–Vis spectroscopy, achieving first explosive therapeutic drug availability. Antifungal evaluations showed that the system effectively inhibited the growth with a minimum inhibitory concentration (MIC) of 30 µg/mL: according to clinical and laboratory standards institute (CLSI). Cytotoxicity assays indicated significantly enhanced biocompatibility compared to free AmB, attributed to the protective alginate matrix. In vivo analysis using ICP-OES demonstrated superior localization of magnetic nanoparticles in magnetically targeted lung tissues, underscoring the platform’s potential for precision medicine applications. The integration of biocompatible alginate and magnetic nanoparticles offers a transformative solution for addressing the challenges of antifungal therapy. This platform not only enhances drug targeting and therapeutic efficacy but also aligns with the principles of precision medicine, paving the way for safer and more effective treatments for pulmonary fungal infections.
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Cell Death & Disease
·
2025
·Vol. 16
·期 1
·1-16
·
IF 9.6
Jing Yukai,Zhang Yunfei,Wang Xiaocui,Wang Yufen,Hu Ying,Wen Bin,Dai Xin,Duan Xuemei,Li Haonan,Dong Shumin,Yan Ze,Fan Yufeng,Wang Cong-yi,Liu Xiansheng,Zhang Ruiping
Systemic lupus erythematosus (SLE) is a typical autoimmune disease characterized by the overproduction of autoantibodies and type I interferon, which damages its own tissues, causing multiple organ damage. B cells are thought to play a major role in the pathogenesis of SLE. As a DNA methylation reader, Methyl-CpG-binding domain protein 2 (MBD2) has been extensively studied in the contexts of innate immunity, adaptive immunity, and autoimmune diseases. However, its specific role in B cells and SLE remains unexamined. Herein, we found that MBD2 was highly expressed in B cells of SLE patients and positively correlated with disease activity. Knockout of MBD2 in B cells disturbed B-cell differentiation, dampened B-cell activation, B-cell receptor (BCR) signaling, and T-cell-dependent humoral immune responses in mice. What’s more, MBD2 deficiency effectively attenuated lupus-like symptoms, reduced the germinal center responses, and decreased anti-dsDNA antibodies in lupus model mice. Mechanistically, MBD2 selectively bound to the methylated CpG of Lef-1 induced by IFN-α, inhibiting the transcription and expression of Lef-1 , which repressed Pten transcription and expression, thereby promoting PI3K-Akt-mTOR signaling. This study first demonstrated the role of MBD2 in the pathogenesis of SLE and provided a new target for SLE.
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ANTIVIRAL RESEARCH
·
2026
·
IF 4
Yarong Song,Yitong Zeng,Han Zheng,Shu Shi,Zhongqing Li,Mingchen Liu,Sijin Shao,Ziheng Luo,Yurong Li,Jie Li,Kuanhui Xiang,Jie Wang
Chronic hepatitis B virus (HBV) infection is a major global health problem. Currently, existing antiviral drugs are difficult to achieve a functional cure for chronic hepatitis B (CHB). Therefore, it is necessary to develop new antiviral targets, especially those that can directly target HBV covalently closed circular DNA (cccDNA) in hepatocytes. Here, we identified a conserved SOX6 binding site in the enhancer I (ENI) region of the HBV genome, and found that SOX6 promotes the replication of multiple genotypes HBV through its HMG domain. Mechanistically, SOX6 binds to the conserved binding site located in the HBV ENI region through its HMG domain, thereby promoting HBV replication by enhancing the transcriptional activity of ENI. Moreover, cisplatin and doxorubicin not only promote HBV replication but also promote SOX6 expression. Knocking down the expression of endogenous SOX6 or mutating the SOX6 binding site in the HBV ENI region significantly weakens the direct promoting effect of cisplatin and doxorubicin on HBV replication. In summary, SOX6 promotes HBV replication by enhancing the transcriptional activity of HBV ENI through its HMG domain, suggesting that it can serve as a potential target for regulating HBV cccDNA transcription. In addition, SOX6 participates in the direct promotion of HBV replication by cisplatin and doxorubicin, providing new insights into the molecular mechanisms of tumor chemotherapy related HBV reactivation (HBVr) and potential targets for the prevention of HBVr during tumor chemotherapy.
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Journal of Materials Research and Technology-JMR&T
·
2024
·
IF 6.2
Xinyue Mao,Qingnan Meng,Sifan Wang,Shiyin Huang,Mu Yuan,Yuting Qiu
To effectively inhibit the thermal oxidation and thermal failure of diamonds when working in a high–temperature aerobic environment, a Mo–B–C coating was designed and prepared to protect diamonds from oxidation. A two–step synthesis method was used to form coatings with different B contents on diamonds by adjusting the reaction temperature, and the oxidation kinetics and oxidation mechanism of the coated diamonds were investigated in the temperature range of 700°C–1200 °C. The mechanism of diamond protection in this study was the preferential oxidative sacrifice of Mo–B–C coatings to form a stable oxide layer on the diamond surface. For low–B coatings, the four stages of coating oxidation, low-temperature volatilization of MoO 3 , stable protection by B 2 O 3 , and rapid evaporation of B 2 O 3 were experienced sequentially with an increase in temperature. For high–B coatings, the preferential self-healing flow of B 2 O 3 not only inhibited the volatilization of MoO 3 but also provided a reducing environment for MoO 3 to generate high melting–point MoO 2 and Mo 2 C, which resulted in the formation of a double synergistic protective oxide layer and greatly enhanced the oxidation resistance of diamonds. Meanwhile, the protection of the oxide layer also maintained the compressive strength of diamonds under a high–temperature oxidizing atmosphere, indicating its excellent high temperature applicability. This study provides an effective method for enhancing the high–temperature oxidation resistance and strength of diamonds, which can be effectively applied to the extreme environmental use of diamond tools.
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BIOMACROMOLECULES
·
2012
·
IF 5.479
Longlong Chang,Liandong Deng,Weiwei Wang,Zesheng Lv,Fuqiang Hu,Anjie Dong,Jianhua Zhang
A novel biodegradable amphiphilic diblock copolymer methoxy poly(ethylene glycol)-b-poly(ε-caprolactone-co-γ-hydroxyl-ε-caprolactone) (mPEG-b-P(CL-co-HCL)) bearing pendant hydroxyl groups on the PCL block was prepared. The hydroxyl groups were formed through the reduction of ketones by sodium borohydride without protection and deprotection. The obtained polymers were well characterized by 1H NMR, Fourier transform infrared (FT-IR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and contact angle measurement. mPEG-b-P(CL-co-HCL) could self-assemble into stable nanoparticles (NPs) with critical micellar concentrations (CMC) of 6.3 × 10–4 ∼ 8.1 × 10–4 mg/mL. The NPs prepared from mPEG-b-P(CL-co-HCL) were spherical in shape with diameters about 100 to 140 nm. The hydrophobic doxorubicin (DOX) was chosen as a drug model and successfully encapsulated into the NPs. The encapsulation efficiency and release kinetics of DOX were investigated. The results indicated that the introduction of hydroxyl groups onto the core-forming block could decrease the hydrophobicity of copolymers, thus improving the storage stability of NPs in aqueous solution. Moreover, higher loading capacity and slower in vitro release of DOX were observed, which was due to the hydrogen-bonding formation between DOX and hydroxyl groups. Meanwhile, the MTT assay demonstrated that the blank NPs were biocompatible to HepG2 cell,s while free DOX and DOX-loaded NPs showed significant cytotoxicity against the cells. Moreover, Compared to the free DOX, the DOX-loaded NPs were more efficiently internalized by HepG2 cells. In sum, the introduction of hydroxyl groups on the polyester block in mPEG-b-P(CL-co-HCL) exhibited great potentials for modifications in the stability, drug solubilization, and release properties of NPs.
研究领域:
高分子科学
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药物递送
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纳米医学
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生物材料
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制药技术
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危险品化学品经营许可证（带存储）