COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
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IF 5.268
Qiang Li,Dongsheng Fu,Jie Zhang,Hong Yan,Huifang Wang,Baolong Niu,Ruijie Guo,Yiming Liu
In this study, a new type of polypeptide, crosslinked methoxy poly(ethylene glycol)-g-poly(aspartic acid)-g-tyrosine (CPPT), was synthesized via a green and simple one-pot polymerization method. With the disulfide-crosslinked interlayer and the CaP shell, the pH and redox dual-sensitive polypeptide-based organic-inorganic hybrid nanoparticles encapsulated curcumin (Cur) into the hydrophobic core of micelles and loaded doxorubicin hydrochloride (DOX) on the hydrophilic segment of micelles as well as CaP shell. The spherical Cur- and DOX-loaded nanoparticles (CPPT@CaP-CD) showed a hydrodynamics size of about 157.9 ± 3.9 nm. The premature leakage of drugs from the nanoparticles at physiological pH was efficiently restrained because of the enhanced structure integrity, whereas at acidic and hypoxia microenvironment the release of both drugs was promoted due to the rapid dissolution of the CaP shell and the break of the disulfide crosslinked network, facilitating the stimuli-responsive controllable drugs release. In vitro anticancer activity evaluation revealed that the co-loaded nanoparticles presented higher cytotoxicity against A549 cells compared with that of the free combination of Cur + DOX. Confocal laser scanning microscopy observation indicated that more DOX and Cur were released into the nucleus triggered by the up-regulated intracellular glutathione (GSH) concentration and decreased pH, displaying enhanced cell uptake. The self-assembling polypeptide-based dual-sensitive drug co-delivery system could be a promising platform for efficient chemotherapy.
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JOURNAL OF BIOMATERIALS APPLICATIONS
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2021
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IF 2.646
Qiang Li,Dongsheng Fu,Jie Zhang,Tianyang Li,Huifang Wang,Wenjuan Hou,Baolong Niu,Ruijie Guo,Yiming Liu
Encapsulation of therapeutic molecules into nanocarrier is an extensively explored strategy to treat cancer more effectively. In this study, pH-responsive targeting dual-agent delivery nanoparticles were prepared, into which hydrophilic doxorubicin hydrochloride (DOX) and hydrophobic curcumin (CUR) were entrapped. Tyrosine (Tyr) was grafted onto poly(aspartic acid) (PASP) to produce PASP-Tyr, the following reaction between hyaluronic acid (HA) and ethylenediamine (EDA) modified PASP-Tyr formed the nanocarrier HA-EDA-PASP-Tyr (HEPT), and the loading capacity was up to 50.9 ± 4.3% for CUR and 26.0 ± 1.9% for DOX. The spherical HEPT with the mean particle size of 142.9 ± 11.4 nm expanded and deformed into petaloid pattern with an increased size of about 2 µm when triggered by the acidic microenvironment. In vitro anticancer activity evaluation revealed that the co-loaded (DOX+CUR)@HEPT nanoparticles presented higher cytotoxicity against HCT-116 cells compared with that of the free combination of (DOX+CUR). Confocal laser scanning microscopy observation indicated that HEPT carrier promoted cellular uptake of drugs by means of active targeting capacity of HA ligand. With high loading capacity and tailored carrier structure, the nanoparticles formulations may offer a new strategy for cancer treatment.
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DRUG DEVELOPMENT RESEARCH
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2020
·Vol. 82
·期 3
·404-411
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IF 1.902
Xiaolong Sun,Xu Wang,Zhenyu Zhao,Jing Chen,Cheng Li,Gang Zhao
Diabetic foot ulcer (DFU) is an invariably common complication of diabetes, characterized by delayed wound healing process and increased inflammation. Evidence has indicated that paeoniflorin exerts an anti-inflammatory effect in diabetic retinopathy. The current work was aimed to investigate the effect of paeoniflorin on inflammation and wound healing in DFU. DFU rat models by streptozotocin and skin biopsy punch, as well as high glucose-treated human immortalized keratinocytes (HaCaT) were established. Levels of blood glucose, wound contraction and proinflammatory cytokine were detected after paeoniflorin administration. Several essential targets associated with the NF-κB and Nod-like receptor protein-3 (NLRP3) signaling pathways were examined. Results showed markedly down-regulation of interleukin (IL)-1β, IL-18 and tumor necrosis factor-alpha in paeoniflorin-treated DFU rats. Paeoniflorin decreased the expression levels of chemokine receptor CXCR2, nuclear NF-κB and p-IκB (Ser36), as well as increased IκB level. Histological analysis and immunostaining showed lower inflammatory cells with decreased NLRP3 and cleaved caspase-1 levels following paeoniflorin treatment. Further in vitro evidence confirmed that paeoniflorin efficiently inhibited NLRP3 and NF-κB-mediated inflammation in DFU by inhibiting CXCR2. These findings are suggestive of greatly attenuated wound inflammation and better wound healing in paeoniflorin-treated DFU rats. Our study demonstrates that paeoniflorin is a potential therapeutic agent for DFU.
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PEDIATRIC HEMATOLOGY AND ONCOLOGY
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2020
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IF 1.232
Ju Yang,Ping Liu,Dan Ma,Peng Zhao,Yan Zhang,Yinghao Lu,Yanju Li,Yi Huang,Ying Chen,Jishi Wang
Development of chemo‑resistance is ultimately responsible for treatment failure and relapse in B-cell acute lymphoblastic leukemia (B-ALL). However, the mechanism underlying glucocorticoid (GC) resistance remains unclear. This study was performed to identify GC resistance-related genes using the transcriptome chip from the GEO database, and preliminarily analyze drug resistance mechanism in B-ALL. Here, we found that ANXA5 expression was upregulated in B-ALL cells and high-level ANXA5 was associated with dexamethasone (DEX) resistance. Then, small interfering RNA (siRNA) was designed to silence ANXA5 expression in the B-ALL cell lines, and the apoptotic rate of cells treated with DEX was detected by flow cytometry. As a result, cell apoptosis was dramatically promoted in B-ALL cells following silencing of ANXA5 and DEX administration versus that in ANXA5-silenced alone or DEX-treated alone cells. It was further found that down-regulation of ANXA5 in B-ALL cells significantly increased the relative amount of cleaved Caspase 3 and Caspase 9 induced by DEX. Collectively, inhibition of ANXA5 gene expression may represent a novel method to restore the sensitivity of treatment-resistant B-ALL tumors to GC-induced cell death, which is of important clinical significance to overcome drug resistance associated with B-ALL.
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Journal of Food Measurement and Characterization
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2020
·Vol. 14
·期 4
·2087-2098
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IF 1.648
Ntakatsane Moeketsi,Chen Ping,Liu Jingsheng,Xu Linlin,Dong Na,Mosebi Poloko,Adoko George,Matebesi Puleng
Antibiotic residues in animal-derived food products such as milk pose a potential health risk to consumers. A portable antimicrobial screening test plate was fabricated particularly for on-farm testing of milk. A cold-water soluble compound gelling agent composed of guar gum (GG) and konjac glucomannan (KG) was prepared and its properties were optimized for enhancing performance of the test plate. A ratio of 4:6 (GG:KG) showed improved gel strength, cohesiveness, transmittance, and water diffusion of the compound gel. Microbial colony number and size were also increased. The test plate displayed a minimum inhibition concentration of 4 µg/L penicillin G and clearer zone of inhibition. Detection time was also significantly reduced to approximately 9–15 h compared to the conventional agar plate method. The plastic film test plate with optimized compound gel demonstrated tremendous potential for detection of antibiotics in milk.
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JOURNAL OF SEPARATION SCIENCE
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2020
·Vol. 43
·期 11
·2105-2114
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IF 2.878
Can Wu,Nani Wang,Pingcui Xu,Xuping Wang,Dan Shou,Yan Zhu
Cell membrane chromatography is a powerful tool for screening active components from complex matrices. New cell membrane carriers need to be developed to increase the coverage of cell membranes on the surface of stationary phases, thereby improving cell membrane chromatographic retention. In this work, we prepared polyvinyl alcohol-poly dimethyl diallyl ammonium chloride modified silica gel as a cell membrane carrier. Osteoblast cell was used as cell membrane source, which was widely used to evaluate the osteogenic activity of drugs. The new cell membrane chromatographic stationary phase was used to screen anti-osteoporosis components in Liuwei Dihuang decoction-containing serum. The chemical structures of the new modified materials were characterized by scanning electronic microscopy, Fourier transform infrared spectroscopy, and elemental analysis characterization. Compared with the common cell membrane column, the cell membrane coverage of this modified material was increased by 30%, and thus provided a stronger retention effect in positive drugs. Nineteen metabolites in rat serum samples were retained on the cell membrane chromatography and identified by ultra-high-performance liquid chromatography/time-of-flight mass spectrometry. Among those, four components (morroniside, catalpol, loganin, and acteoside) were selected for in vitro pharmacodynamics validation. They significantly increased the osteoblast proliferation. The new modified material was successfully applied to screen anti-osteoporosis components from Liuwei Dihuang Decoction-containing serum.
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GLYCOCONJUGATE JOURNAL
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2020
·Vol. 37
·期 3
·361-371
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IF 2.197
Zhang Wuxia,Hu Yihua,Zhao Jinzhong,Zhang Yongpo,Guo Dongdong,Gao Chunyan,Duan Jinyou,Li Peng
Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, immunomodulatory, cancer, and other diseases. In this paper, a novel acidic polysaccharide RPAPS purified from Radix Paeoniae Alba was evaluated for its structural features and potential of immunomodulatory and antioxidant activities. RPAPS (molecular weight: 1.0× 10 5 Da) was mainly composed of α-(1 → 4)-Glc p , α-Ara p , α-Gal p , α-Rha p , β-D-Glc p , α-(1 → 6)-linked Glc p and GalA. Immunological tests indicated that RPAPS could improve RAW264.7 phagocytic activity and LPS-induced splenocyte proliferation. For antioxidant activities, RPAPS showed reducing power and DPPH scavenging activity in dose dependent. Moreover, RPAPS could significantly protect the PC12 cells from H 2 O 2 damage. These data implied polysaccharides RPAPS had the potential to be novel natural antioxidative and immunopotentiating agents for using in functional foods or medicine.
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SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Di Zhu,Zhenyuan Bai,Huiyuan Ma,Lichao Tan,Haijun Pang,Xinming Wang
Developing a high-efficient and low-cost nanocomposite, featured by abundant active sites with excellent transfer ability for electrons and high conductivity, for the simultaneous detection of biomolecules belonging to different systems is critically important and challenged. Herein, a nanocomposite composed of ferroferric oxide nanoparticles (Fe 3 O 4 ) functionalized polyoxometalates (H 6 PMo 9 V 3 O 40 ·nH 2 O) was synthesized, using one-step hydrothermal doped method. It exhibited high electrocatalytic activity for oxidation of β-nicotinamide adenine dinucleotide (NADH) and l -tryptophan ( l -trp) with well separated signals (Δ =180 mV) between them coming from the combination of PMoV and Fe 3 O 4 . The PMoV@Fe 3 O 4 /GCE-based sensor exhibited outstanding sensing capability for NADH and l -trp simultaneously with low detection limit of 53 nM and 17 nM, respectively, and excellent selectivity over various possible interfering species, which originated from the synergistic effect of PMoV and Fe 3 O 4 . Moreover, the practical application potential of the PMoV@Fe 3 O 4 sensor was successfully demonstrated by determining NADH and l -trp in real samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Jian Li,Debao Niu,Ying Zhang,Xin-An Zeng
The influences of different extraction methods on the yields, physicochemical properties, antioxidant activities and antiproliferative abilities of polysaccharides from Morinda citrifolia L. (Noni) were investigated and compared. Three extraction methods were applied to extract Noni polysaccharides (NP), which include hot water extraction (HWE), pulsed electric field-assisted extraction (PEFAE) and ultrasonic-assisted extraction (UAE). The extraction yields, sugar contents, uronic acid contents, molecular weights, monosaccharide proportions, antioxidant activities and antiproliferative abilities of three NP samples were significantly different. However, their preliminary structural characteristics and monosaccharide types were similar. More specifically, UAE-NP had the highest extraction yield, the smallest molecular weight and the best antioxidant activities. Especially, UAE-NP showed excellent antiproliferative abilities. Compared with HWE-NP and PEFAE-NP, the IC 50 value of UAE-NP on HepG2 cells reduced by 45.45% and 33.14%, respectively. The highest antioxidant activities of the UAE-NP might be attributed to its smaller molecular weight, lower Gal content and higher Fuc content. The strongest antiproliferative effects of the UAE-NP might be related to the low molecular weight. Overall, UAE could be a potential technique for the extraction of high-quality NP due to its high yield, high efficiency and notable bioactivities.
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JOURNAL OF SOLID STATE CHEMISTRY
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2020
·
IF 2.726
Yumei Xiao,Dongning Liu,Cheng Liu,Yongchao Wang,Cuijuan Wang
Curcumin (CCM) is a natural ingredient with anti-cancer activity. However, the application of CCM is limited in clinical medicine due to its high hydrophobicity and low bioavailability. Hence, it is necessary for development of a feasible strategy for efficient drug delivery and cancer cell-selective targeting. In recent years, nano-scaled metal-organic frameworks (NanoMOFs) have received much attention as an attractive drug delivery carrier due to their unique features of their various sizes, adjustable pore structures , high stability, and easy surface modification. Here, we report a novel composite nanomaterial (denoted as ZIF-8&α-lip) for CCM loading by one-pot method. This synthetic method is simple and rapid, and the final product (CCM@ZIF-8&α-lip) can be obtained within 20 min. In addition, the obtained CCM@ZIF-8&α-lip shows greater growth inhibition of HepG2 cells in comparison with CCM@ZIF-8. Therefore, ZIF-8&α-lip can be used as an ideal drug carrier and propose a new strategy to improve nanoMOFs performance for efficient drug delivery.
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Pharmaceutics
·
2020
·Vol. 12
·期 7
·675
·
IF 4.421
Chun Chu,Zhihong Bao,Meng Sun,Xiaowei Wang,Hongyan Zhang,Weiguo Chen,Yang Sui,Ji Li,Yuanyuan Zhuang,Dongkai Wang
The combination of chemotherapy and phototherapy has attracted increasing attention for cancer treatment in recent years. In the current study, porous PdPt bimetallic nanoparticles (NPs) were synthesized and used as delivery carriers for the anti-cancer drug doxorubicin (DOX).[email protected]NPs were modified with thiol functionalized hyaluronic acid (HA-SH) to generate[email protected]@HA NPs with an average size of 105.2 ± 6.7 nm. Characterization and in vivo and in vitro assessment of anti-tumor effects of[email protected]@HA NPs were further performed. The prepared[email protected]@HA NPs presented a high photothermal conversion efficiency of 49.1% under the irradiation of a single 808 nm near-infrared (NIR) laser. Moreover, NIR laser irradiation-induced photothermal effect triggered the release of DOX from[email protected]@HA NPs. The combined chemo-photothermal treatment of NIR-irradiated[email protected]@HA NPs exerted a stronger inhibitory effect on cell viability than that of DOX or NIR-irradiated[email protected]NPs in mouse mammary carcinoma 4T1 cells in vitro. Further, the in vivo combination therapy, which used NIR-irradiated[email protected]@HA NPs in a mouse tumor model established by subcutaneous inoculation of 4T1 cells, was demonstrated to achieve a remarkable tumor-growth inhibition in comparison with chemotherapy or photothermal therapy alone. Results of immunohistochemical staining for caspase-3 and Ki-67 indicated the increased apoptosis and decreased proliferation of tumor cells contributed to the anti-tumor effect of chemo-photothermal treatment. In addition,[email protected]@HA NPs induced negligible toxicity in vivo. Hence, the developed nanoplatform demonstrates great potential for applications in photothermal therapy, drug delivery and controlled release.Keywords:PdPt bimetallic nanoparticles;drug delivery;chemo-photothermal therapy;anti-tumor effect
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Food Bioscience
·
2020
·
IF 3.067
Xiao-Hui Yao,Yu-Shi Shen,Run-Ze Hu,Ming Xu,Jia-Xing Huang,Chen-Xi He,Fu-Liang Cao,Yu-Jie Fu,Dong-Yang Zhang,Wei-Guo Zhao,Li Liu,Tao Chen
Mulberry is a traditional crop that has been grown in China for thousands of years. While leaves and fruits of the mulberry have been consumed, mulberry seed use has been less extensive. The goal of this study was to investigate the value of the seeds. Seven cultivars of mulberry were investigated. Oil was extracted from seeds, and the oil content was determined. The primary components of the oils were analyzed using gas chromatography . It was found that all the mulberry cultivars contained high linoleic acid contents, suggesting that mulberry seed may be a potential resource of linoleic acid. The total phenolic and flavonoid contents were also compared among different mulberry seed oils . The physical and chemical parameters, as well as the antioxidant activity of the mulberry seed, were determined. Principal component analysis indicated that the ferric ion reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity (DPPH) of mulberry seed oil may be due to high phenolic compounds and linoleic acid. The cultivars Dongsheng, Fengtian, and Guisang had the highest antioxidant activities (FRAP, DPPH), and it was proposed that these cultivars would produce the highest quality oil for use as an ingredient for healthcare or functional food use.
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