Food Chemistry-X
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2026
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IF 9.2
Haijun Chang,Xin Wen,Yu Huang,Yu Hu,Yuanwei Shi
The research aimed to elucidate the interaction and mechanism between the Portulaca oleracea L. extract (POE) and the pork myofibrillar proteins (MPs) from the perspectives of fluorescence spectroscopy and molecular docking. The results showed that POE significantly increased intermolecular hydrogen bonds and hydrophobic interactions in MPs ( P < 0.05). Among the non-covalent forces between POE and MPs, hydrophobic interactions were dominant followed by hydrogen bonds. POE would cause a decrease in the endogenous fluorescence intensity of MPs, and the quenching type was static quenching. The binding molar ratio of POE to MPs was 1:1, and the formed complex exhibited high stability. The thermodynamic constants ∆G < 0, ∆H > 0, and ∆S > 0 indicated that the binding process between POE and MPs was spontaneous, with hydrophobic interactions being the primary driving force. Additionally, the nine most abundant compounds in POE were selected for molecular docking with myosin, and the binding energies were all negative, confirming that POE could spontaneously interact with MPs. The molecular docking results further demonstrated that hydrophobic interactions and hydrogen bonding were the main binding forces, with Leu270 potentially serving as a key amino acid residue in the interaction between POE and MPs. This study provides valuable insights into the molecular interactions between Portulaca oleracea L. extract and pork myofibrillar proteins, offering a theoretical basis for phenolic small molecule substances in regulating protein oxidation and potential applications in improving meat product quality.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2013
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IF 2.672
Yinfeng Li,Lina Zou,Ge Song,Kunjing Li,Baoxian Ye
The electro-polymerization of l -Theanine was investigated for the first time on glassy carbon electrode and the polymeric conditions and mechanism were discussed in detail. Hereafter, a simple and sensitive electrochemical sensor based on the poly( l -Theanine) film was established for sophoridine sensing. The electrode process of sophoridine was then studied by cyclic voltammetry and its determination was achieved by amperometry . The results revealed that the poly( l -Theanine) membrane effectively decreased the oxidation potential of sophoridine and greatly increased its response current. And this sensor, fabricated easily and simply as well as very easy surface update, showed good response for sophoridine with a wide linear range from 1.0 × 10 −6 to 1.4 × 10 −4 mol L −1 and a low detection limit of 5 × 10 −7 mol L −1 . The application of proposed method in analysis of real sample was also evaluated with good performance. This work promoted the potential applications of amino acid materials in electrochemical sensors.
研究领域:
分析化学
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电化学
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药物分析
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生物传感
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分子诊断
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药物代谢
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.5
Xinkai Sun,Xinying Jiang,Fei Xie,Pengju Yao,Yi Zhang,Hui Yang,Carla Bittencourt,Wenjiang Li
In the context of sustainable development, the exploration of renewable, biodegradable, and environmentally friendly polymer aerogels have emerged as a significant area of research. Herein, we report a facile synthesis of fully degradable polylactic acid (PLLA)/poly (butylene adipate- co -terephthalate) (PBAT) composite aerogels via 1,4-dioxane-assisted co-dissolution, followed by freeze-drying and phase separation techniques. The resulting aerogels feature a hierarchically porous structure with 98.6% porosity and vertically aligned channels, formed by controlled solvent crystallization during the freezing process. This unique architectural configuration integrates PLLA's oil-retention capability with PBAT's elasticity, yielding adsorption capacities of 13.6 g/g for n-hexane and 31.5 g/g for carbon tetrachloride. Notably, the aerogel retains ~97.5% stress and height recovery after 100 cycles of 50% strain compression, overcoming pure PLLA's inherent brittleness. It also maintains 96.5% the oil-water separation efficiency after 20 c centrifugal desorption cycles. 1,4-Dioxane promotes the compatibility of PLLA and PBAT, improving the interfacial compatibility and effectively avoiding the structural defects usually caused by phase separation. This research provides a scalable, filler-free approach to fabricate the high-performance biodegradable aerogels, bridging environmental sustainability and practical functionality for oily wastewater remediation.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2026
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IF 4.7
Li Zhou,Xianwei Jiao,Yuting Zhang,Changling Yan
L-selectin, a leukocyte adhesion receptor, is essential in the early step of the adhesion cascade, facilitating the migration of leukocytes into surrounding tissues during inflammation and immune surveillance. Clinical studies have identified L-selectin as a prospective treatment option for many both acute and ongoing inflammatory conditions. Clarifying the cellular signaling pathways that function in the immediate vicinity of L-selectin may yield unique treatments tailored to certain cell types or illnesses. The study describes the development of a precise and sensitive fluorescence biosensor designed for the detection of L-selectin using a split aptamer specific to L-selectin. This biosensor utilizes nicking enzyme-assisted DNA walking together CRISPR-Cas12a for dual-signal multiplication. The calibration curve for L-selectin demonstrates a detection limit of 0.45 ng/mL, with an extensive linear range spanning from 10 to 1280 ng/mL. The fluorometric sensors effectively identified L-selectin in human blood samples, producing excellent outcomes. The fluorometric sensors effectively identified L-selectin in human blood samples, producing excellent outcomes. The original appearance not only validates the concept but also possesses substantial possibilities for identifying diverse targets.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Yantong Lu,Yue Li,Ji Zhang,Qianhong Shen,Hui Yang
Integrating robust physical barrier properties with efficient self-healing capability is widely recognized as an effective strategy for developing long-term anticorrosive coatings. In this study, a pH-responsive multifunctional nanocontainer, denoted as ZIF-L-BTA@PDA (ZBP), was rationally designed through in situ encapsulation of benzotriazole (BTA) within two-dimensional leaf-like ZIF-L, followed by dopamine (DA) self-polymerization to form a polydopamine (PDA) shell on the ZIF-L-BTA surface. The resulting ZBP nanocontainers were uniformly dispersed in a waterborne epoxy (WEP) matrix to fabricate a composite coating with enhanced barrier performance, intelligent self-healing behavior, and improved interfacial compatibility. In this system, ZIF-L serves a dual function as both an inhibitor reservoir and a high-aspect-ratio barrier filler, effectively prolonging diffusion pathways for corrosive species. The PDA shell endows the nanocontainer with pH-triggered BTA release behavior, additional self-healing capability, and the improved compatibility with the epoxy matrix. Release studies confirmed the pH-responsive characteristics of the ZBP nanocontainers. After 40 days of immersion in a corrosive medium, the ZBP/WEP coating maintained a low-frequency impedance modulus (|Z| 0.01Hz ) value of 1.08 × 10 10 Ω·cm 2 , approximately three orders of magnitude higher than that of pure WEP (1.34 × 10 7 Ω·cm 2 ), corresponding to an anticorrosion efficiency of 99.99%. The self-healing performance of ZBP/WEP was further validated by artificial scratch test. This work provides a promising strategy for designing high-performance, intelligent self-healing coatings for long-term corrosion protection.
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Zhuoshuo Zhou,Weirong Sun,Liye Yang,Yue Li,Hongpeng Guo,Ximing Cui,Yanli Zhao,Jinlian Li,Gang Wang,Dongmei Wu
Ethnopharmacological relevance Physalis alkekengi L. ( P. alkekengi ) is widely used in traditional medicine and functional foods for its various medicinal properties. However, its toxicological profile has not been comprehensively assessed. Aim of the study This study aimed to characterize the developmental toxicity and cardiotoxicity of P. alkekengi using a Danio rerio ( D. rerio ) embryo model and to identify key toxic constituents to support safety optimization. Materials and methods D. rerio embryos were exposed to the fruit and calyx extracts of P. alkekengi as well as membrane-fractionated preparations to assess toxic phenotypes. Chemical profiling was performed using ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-Exactive Orbitrap-MS), and mechanistic insights were explored using apoptosis-related assays and network toxicology analysis. Results The fruit extract of P. alkekengi exhibited stronger developmental toxicity and cardiotoxicity effects compared to the calyx extract. The 5–10 kDa non-polysaccharide fraction of the fruit was identified as the primary toxic source, with Glycidyl stearate (GS) being the key cardiotoxic component in this fraction. Removal of the most toxic 5–10 kDa fraction during the extraction process significantly reduced the toxicity of the fruit extract. Conclusion The toxicity of P. alkekengi fruit extract is concentrated in the non-polysaccharide 5–10 kDa fraction, with GS being the main contributor to the toxicity in this fraction. Membrane-based separation technology to remove this fraction can enhance the safety of the extract. This study provides a framework for the safety evaluation and optimization of traditional medicinal plants.
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Analytical Methods
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2026
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IF 3
Huili Luo,Yuhang Xiang,Senbin Qin,Xiaopeng Pan,Anxin Li,Jia Zhao,Qiang Fu,Dengting Yang
Thiamethoxam (TMX), a widely used neonicotinoid insecticide, is electrochemically detected based on the reducibility of the nitro group. However, complex electrodes and matrix interferences limit the application. Due to the response attenuation of the graphite paper electrode (GPE) toward TMX detection, Nafion solution (NF) and L-ornithine hydrochloride (LO) were adopted to improve the measurement stability of TMX and enhance the resistance to matrix interferences. After drip-coating modification, a new electron-conducting layer was formed, accompanied by a scaly film with stacked interlayer gaps of approximately 100 nm. LO acted as a bridge, which bonded with NF via a sulfonylation reaction and linked to TMX through C–O–NO2 during the electro-sorption and enrichment process. This binding to TMX corresponded to a reversible electrochemical process involving one-electron transfer. Accordingly, the analytical stability was greatly improved. The modified NF–LO/GPE also exhibited excellent anti-interference ability against extractable organic acids from celery, such as chlorogenic acid. This was mainly attributed to the negative charge repulsion of NF and acid-base neutralization under moderate alkaline activation. This work demonstrates good modifiability of NF–LO based on the sheet-like graphite electrodes and conducts a valuable exploration of matrix interference in real samples. Enhancing the adsorption capacity of the electrode surface by constructing unsaturated C–O sites may be the primary issue for the detection of organic pesticides.
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Journal of Essential Oil Bearing Plants
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2026
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IF 2.1
Boyang Zhang,Xuyang Hei,Wei Zhang
Microreactor technology has demonstrated significant advantages in natural product extraction due to its superior mass transfer
efficiency. This study optimized the extraction of essential oil from L. cladonioides leaves using a continuous flow microreactor
through Box-Behnken experimental design. An extraction yield of 1.81% was achieved under optimal conditions (74 μm powder,
43°C, 2.5 h, ethanol solvent). GC-MS analysis identified 29 compounds accounting for 97.59% of total content, predominantly
terpenoids (91.33%), with α-pinene (24.88%), β-pinene (19.65%), and p-mentha-1,8-diene (16.97%) as major constituents. Eight
compounds, previously unreported, were detected in this study. The essential oil demonstrated potent antibacterial activity against
Gram positive (S. aureus MIC = 1.4 μL/mL), Gram negative (E. coli MIC = 1.5 μL/mL) bacteria, and fungi (C. albicans MIC
= 1.7 μL/mL), exhibiting superior antifungal efficacy compared to amoxicillin. Antioxidant assays revealed moderate DPPH
radical scavenging capacity (IC50 = 37.69 μg/mL) and β-carotene/linoleic acid system inhibition (IC50 = 0.77 mg/mL). Antibacterial
mechanisms involved disruption of cell membrane integrity, as evidenced by extracellular nucleic acid leakage. Cytotoxicity testing
confirmed safety for HEK293T cells at concentrations ≤ 50 μg/mL. These findings demonstrate that microreactor extracted L.
cladonioides essential oil represents a natural antimicrobial and antioxidant agent with low cytotoxicity, demonstrating promising
applications.
Keywords: Lethariella cladonioides (L. cladonioides), Essential oil, Composition identification, Antibacterial activity, Continuous
flow microreactor
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COLLOID AND POLYMER SCIENCE
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2026
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IF 2.9
Qi Hexu,He Qinglin,Guo Yunfeng,Sun Xin,Zhao Zhihe
Achieving simultaneous mechanical robustness and cytocompatibility in polymer-based scaffolds requires precise interfacial regulation at the nanoscale. Herein, graphene oxide-hydroxyapatite (GO-HA) nanohybrids were engineered via three distinct surface modification strategies—tannic acid (TA) adsorption, polydopamine (PDA) deposition, and poly(D-lactide) (PDLA) grafting—to elucidate how interfacial chemistry governs structural evolution and composite performance in Poly(L-lactic acid) (PLLA) matrices. While all modifications preserved hydroxyapatite crystallinity, they induced differentiated interphase architectures: TA introduced molecular-level surface functionalization with minimal aggregation, PDA formed a conformal interphase layer, and PDLA generated polymer-rich encapsulated structures with enlarged dispersion units. Thermal analysis revealed that PDLA grafting maximized crystallinity and thermal stability due to molecular compatibility with PLLA. However, mechanical evaluation demonstrated that GO-HA-TA achieved superior reinforcement, highlighting the importance of balancing interfacial adhesion with polymer chain mobility. Excessive interphase thickening, despite enhancing crystallization, may constrain deformation adaptability. Furthermore, TA-modified scaffolds exhibited improved wettability and uniform surface morphology. Cytocompatibility assays confirmed high cell viability and negligible cytotoxicity across all groups. This study establishes that optimizing cytocompatible composite scaffolds requires not merely maximizing interfacial compatibility, but strategically balancing adhesion and structural discreteness, identifying surface modification strategies as the most effective route for high-performance PLLA/GO-HA scaffolds.
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CHEMICAL ENGINEERING SCIENCE
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2023
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IF 4.7
Xin Song,Fangjun Shao,Zijiang Zhao,Xiaonian Li,Zhongzhe Wei,Jianguo Wang
Precise regulation of reaction selectivity at high conversions is the key to non-terminated alkyne semi-hydrogenation. Herein, a support modification strategy was employed to incorporate trace amounts of Mg elements into alumina support. The modified support loaded Pd catalyst optimized the activity and selectivity of the non-terminated alkyne semi-hydrogenation reaction. The TEM and XRD characterization of the catalysts demonstrated the entry of Mg elements into the Al 2 O 3 lattice to achieve the modification of the support. For the 2-butyne-1-ol semi-hydrogenation reaction, the Pd 0.5 /Mg 1 @Al 2 O 3 catalyst showed 99 % conversion and 97 % selectivity of the cis product at hydrogen pressure of 0.1 MPa and 25 °C. The introduced Mg element changed the electronic structure of Pd nanoparticles in the catalyst. The precise regulation of the 2-butyne-1-ol semi-hydrogenation reaction was achieved by adjusting the ratio of Pd 0 to Pd 2+ using the synergistic effect of Pd 0 and Pd 2+ .
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THERIOGENOLOGY
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2023
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IF 2.8
Xu-dong Jiang,Yu Liu,Jian-fei Wu,San-ni Gong,Yao Ma,Xiang-dong Zi
Supplementation with acetyl- l -carnitine (ALC) during in vitro maturation significantly improves the rates of oocyte cleavage and morula and blastocyst formation in sheep and buffalo ; however, the mode of action of ALC in improving oocyte competence is not completely understood. Therefore, the aim of this study was to investigate the effects of ALC on proliferation, antioxidant properties, lipid droplet accumulation and steroid hormone secretion in yak ( Bos grunniens ) granulosa cells (GCs). Yak GCs were identified using FSHR immunofluorescence. The cells were treated with different concentrations of ALC, cell proliferation was detected by cell counting kit-8, and the optimal concentration and treatment time were determined for subsequent experiments. Then, reactive oxygen species (ROS) were detected by a DCFH-DA probe, and lipid droplet accumulation was observed by oil red O staining. Estradiol (E2) and progesterone (P4) in the medium were detected by ELISA, and the expression of genes related to cell proliferation, apoptosis, the cell cycle, antioxidants and steroid synthesis was determined by RT‒qPCR. The results showed that 1 mM ALC treatment for 48 h was the optimum treatment. It significantly increased cell viability ( P < 0.05), significantly decreased the amount of ROS and lipid droplet content, and promoted P4 and E2 secretion ( P < 0.05) of yak GCs. RT‒qPCR results verified that GCs treated with 1 mM ALC for 48 h significantly increased the expression of genes related to anti-apoptosis and the cell cycle ( BCL-2 , PCNA , CCND1 and CCNB1 ), antioxidants ( CAT , SOD2 and GPX1 ), and E2 and P4 secretion ( StAR, CYP19A1 and HSD3B1 ) ( P < 0.05), but it significantly decreased the expression of apoptosis genes ( BAX and P53 ) ( P < 0.05). In conclusion, ALC increased the viability of yak GCs, reduced the amount of ROS and lipid droplets, increased P4 and E2 synthesis and affected the expression of related genes in yak GCs.
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ACS Biomaterials Science & Engineering
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2023
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IF 5.8
Shuang Li,Dongyang Niu,Tuhe Shi,Wentao Yun,Shifeng Yan,Guohua Xu,Jingbo Yin
Injectable hydrogels have drawn much attention in the field of tissue engineering because of advantages such as simple operation, strong plasticity, and good biocompatibility and biodegradability. Herein, we propose the novel design of injectable hydrogels via a Schiff base cross-linking reaction between adipic dihydrazide (ADH)-modified poly(l-glutamic acid) (PLGA-ADH) and benzaldehyde-terminated poly(ethylene glycol) (PEG-CHO). The effects of the mass fraction and the molar ratio of −CHO/–NH2 on the gelation time, mechanical properties, equilibrium swelling, and in vitro degradation of the hydrogels were examined. The PLGA/PEG hydrogels cross-linked by dynamic Schiff base linkages exhibited good self-healing ability. Additionally, the PLGA/PEG hydrogels had good biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and could effectively support BMSC proliferation and deposition of glycosaminoglycans and upregulate the expression of cartilage-specific genes. In a rat cartilage defect model, PLGA/PEG hydrogels significantly promoted new cartilage formation. The results suggest the prospect of the PLGA/PEG hydrogels in cartilage tissue engineering.
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