Su Kaiyue,Peng Xinli,Zheng Yuze,Qiao Xuguang,Waterhouse Geoffrey I.N.,Xu Zhixiang
In this paper, a molecularly imprinted polymer with good benzopyrene adsorption performance and a PDA@Mn-ZIF-8 nanozyme with high catalytic activity were synthesized. The molecularly imprinted polymer was used as the biomimetic antibody, while the PDA@Mn-ZIF-8 was used as the marker. A novel direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method was established for the sensitive detection of benzopyrene. Under the optimal conditions, the detection limit (LOD and IC 15 ) and sensitivity (IC 50 ) of the BELISA method for benzopyrene were 0.52 µg L −1 and 65.64 µg L −1 , respectively. This method was successfully used to detect benzopyrene in chicken and pork samples, with recovery rates ranging from 89.12 to 105.26% for chicken and 92.83 to 112.31% for pork. The contents of benzopyrene in sausages and grilled lamb samples were determined by BELISA and HPLC, and no significant differences found between the results of the two methods. This work offers a new, rapid, and accurate method for the detection of benzopyrene in foods.
Fang He,Gang Chen,Yansong Zhou,Yaoguang Yu,Laiquan Li,Sue Hao,Bin Liu
A ZIF-8 derived carbon (C-ZIF)/g-C3N4 composite was constructed for the first time through facile thermal condensation of a zeolitic imidazolate framework (ZIF-8) and melamine. The obtained C-ZIF/g-C3N4 composite exhibited an obviously enhanced photocatalytic H2 production rate compared to pure g-C3N4 under visible light irradiation. The 1 wt% C-ZIF/g-C3N4 composite without loading the Pt co-catalyst showed 36.2 times higher H2 evolution rate than that of pure g-C3N4, which is even 2.8 times higher than that of Pt/g-C3N4 (the state-of-the-art g-C3N4-based photocatalyst). It was revealed by photoluminescence spectroscopy, time-resolved fluorescence spectroscopy and electrochemical impedance spectroscopy that the formed C-ZIF and g-C3N4 junction could promote quick charge carrier separation and transfer. The C-ZIF not only acted as an effective electron acceptor, but also functioned as an efficient hydrogen evolution reaction (HER) cocatalyst to promote photocatalytic hydrogen evolution. Our work provides an effective way for the development of metal-free, all carbon-based photocatalysts for H2 evolution.
A novel and simple ratio fluorescence detection method for H2O2 and glucose was proposed on the basis of the Aggregation-induced luminescence effect of the Aggregation-induced emission (AIE) molecule 4-formyl-3-hydroxybenzoic acid (FHBA) in ZIF-8 and the mechanism of Fluorescence resonance energy transfer (FRET) between the complex FHBA@ZIF-8 and Diallyl phthalate (DAP). First, glucose was oxidized to H2O2 under the catalysis of glucose oxidase. The latter further oxidized o-phenylenediamine in the presence of horseradish peroxidase to generate DAP yellow fluorescent compound (560 nm). The fluorescence of FHBA@ZIF-8 at 445 nm can be effectively quenched because the ultraviolet absorption spectrum of DAP overlaps well with the emission spectrum of FHBA@ZIF-8. Therefore, a highly sensitive ratiometric fluorescence detection method for detecting H2O2 and glucose was constructed using the change of the ratio of fluorescence intensity at two wavelengths (F560/F445). The linear range of H2O2 detection by this method is 1–150 μM, and the detection limit is 0.21 μM. The linear range of glucose detection is 1–100 μM, and the detection limit is 0.17 μM. The method was successfully applied to the detection of glucose in human serum samples, and it has broad potential in clinical diagnosis and research
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