Frontiers in Microbiology
·
2024
·
IF 4
Yinyin Gao,Cheng Li,Junfei Li,Mengyao Duan,Xuan Li,Lina Zhao,Ying Wu,Shaobin Gu
Introduction: Hyperuricemia (HUA) refers to the presence of excess uric acid (UA) in the blood, which increases the risk of chronic kidney disease and gout. Probiotics have the potential to alleviate HUA.Methods: This study established a hyperuricemia model using Caenorhabditis elegans (C. elegans), and studied the anti-hyperuricemia activity and potential mechanisms of Weizmannella coagulans BC99 (W. coagulans) at different concentrations (107 CFU/mL BC99, 108 CFU/mL BC99). Subsequently, we utilized UPLC-Q-TOF/MS to investigate the impact of BC99 on endogenous metabolites in C. elegans and identified pathways and biomarkers through differential metabolomics analysis.Results: The results of this study showed that BC99 treatment significantly reduced the expression of P151.2 and T22F3.3 (p < 0.05), reduced the levels of UA and xanthine oxidase (XOD) in nematodes (p < 0.05), while extending their lifespan and movement ability (p < 0.05). Mechanistically, BC99 activates the transcription factors DAF-16 and SKN-1, thereby inducing the expression of stress response genes, enhancing the activity of antioxidant enzymes and tolerance to heat stress in the body, and reducing the production of ROS (p < 0.001). This effect was most significant in the H-BC99 group. Furthermore, non-targeted metabolomics indicated that BC99 predominantly regulated pathways associated with amino acid metabolism (Carnosine), glycerophospholipid metabolism, and purine metabolism.Discussion: These results underscore BC99 as an effective and economical adjunct therapeutic agent for hyperuricemia, providing a scientific basis for further development and application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yao Ji,Tiangang Ma,Xianwang Yang,Renshuo Wang,Ke Wang,Xiyu Liu,Xiaolong Wang,Quan Jin
SO 2 sensors generally face challenges such as gas corrosion, poor selectivity, competition from water molecules, and high recovery costs. Metal-organic frameworks (MOFs), which offer excellent chemical stability, structural diversity, and low-cost reactivation, have emerged as ideal candidates for sensing materials. However, MOFs typically exhibit poor conductivity, making them unsuitable for traditional gas detection devices. In order to solve this problem, in this study, SIFSIX-1-Cu with directionally ordered interconnected nanochannels was synthesized. By optimizing the synergistic effects of mass transfer, chemical recognition and pore-size sieving, the performance limitations of traditional adsorption materials have been overcome. Furthermore, by integrating SIFSIX-1-Cu with a microwave circuit, the microwave gas sensor (MGS) detects target gases by monitoring changes in the electromagnetic properties—such as dielectric constant or resonance frequency—of the sensing material induced by gas adsorption. The unique detection mechanism allows the MOFs to operate without the need for conductivity optimization, effectively preventing the loss of active sites during the material modification process. The results showed that SIFSIX-1-Cu microwave gas sensor has a low limit of detection of 8.9 ppb at room temperature, and can achieve high selectivity (selectivity coefficient SO 2 /CO 2 > 11.87) detection of SO 2 in a wide concentration detection range of 10 ppb to 1000 ppm. Meanwhile, the sensor also exhibits excellent moisture resistance and repeatability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioengineering
·
2025
·Vol. 4
·期 2
·166-181
·
IF 2.3
Baoming Tian,Xue Zhou,Shuo Pan,Wei Liu
Tea polysaccharides have attracted widespread attention in regulating gut microbiota. A highly efficient enzymolysis and ultrasonic-assisted extraction method was developed and optimized for the extraction of polysaccharides from Zhonghuang No. 1 etiolated-green tea (GTP) with a-single factor and response surface methodology, i.e, with a pH of 5.5, an enzyme concentration of 7%, an ultrasonic time of 2.5 h, a solid-liquid ratio of 1:20 g/mL, and an ultrasonic temperature of 80°C. GTP exhibited a triple helix structure and demonstrated the ability to bind with Congo red. Its surface was observed to be spherical with slender chain fragments. GTP remained intact throughout the simulated in vitro digestive process, which encompassed the oral, gastric, and small intestinal phases. The relative abundance of Bacteroidota was significantly increased, while the Firmicutes/Bacteroidota ratio was reduced following the administration of GTP. The contents of Faecalibacterium and Bacteroides were increased, while the contents of Escherichia_Shigella and Dorea were reduced after GTP treatment. Furthermore, GTP were degraded and utilized by gut microbiota to produce SCFAs and reducing pH value. GTP regulated the composition of the gut microbiota and has the potential to act as a prebiotic, with the possibility of its use as a functional food.
✓ 已验证
产品关联
DOI
高影响因子
Phytochemistry Letters
·
2025
·
IF 1.4
Yan-Peng Liang,Qian Gao,Hong Chen,Rui Ma,Xiao-Bo Zhao,Xiao-Jie Jin,Jian Zhang,Yan-Ping Shi,Wei Ha
Alzheimer’s disease, a progressive neurodegenerative disorder of the central nervous system, presents a complex pathological mechanism that limits the efficacy of conventional single-target drugs. Utilizing the unique advantages of natural products in multi-target intervention, this study employed the active component 1- O -acetylbritannilactone (ABL) from Inula britannica L. as the parent structure. Guided by the multi-target directed ligands (MTDLs) strategy, four novel derivatives (ABL-1 to ABL-4) were successfully designed and synthesized by introducing acetylcholinesterase (AChE) inhibitory pharmacophores into the ABL scaffold. In vitro pharmacological evaluation demonstrated that ABL-2, ABL-3, and ABL-4 not only preserved the anti-neuroinflammatory activity of the parent compound but also endowed compound ABL with AChE inhibitory potency. Notably, ABL-4 displayed the most promising dual efficacy profile, achieving an AChE inhibition rate of 40.09 ± 0.73 % at 30 μM and an anti-neuroinflammatory activity (EC 50 = 8.43 ± 0.5 μM). Molecular docking simulations revealed that the dual-target synergistic mechanism of ABL derivatives arises from hydrogen bonding and π-π stacking interactions with key residues of both AChE and inducible nitric oxide synthase (iNOS). This work not only validates the feasibility of the MTDLs strategy in optimizing natural product scaffolds but also provides a structurally novel lead compound with significant potential for developing multi-target anti-AD therapeutics.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhiyue Wang,Yaru Wang,Shihong Xin,Xueqing Yong,Qixin Wang,Yunlin Chen
Hydrogen (H 2 ) leakage poses critical safety hazards and environmental risks in clean energy systems, driving urgent demands for cost-effective hydrogen sensors with high sensitivity, high selectivity, and rapid response time. Herein, high-performance hydrogen sensor based on photonic crystals (PCs) assembled by CAU-1, nickel (Ni) -doped CAU-1, and TiO 2 are prepared. Comparing with the CAU-1/TiO 2 and CAU-1Ni 15% /TiO 2 PC sensors, CAU-1Ni 25% /TiO 2 PC sensor shows a high sensitivity of hydrogen response to be 0.083 nm/ppm with a good linear relationship in the concentration range of 9–90 ppm hydrogen. Density-functional theory (DFT) adsorption energy calculations quantitatively validate the enhanced hydrogen adsorption capacity through Ni-induced electronic structure modulation. The CAU-1Ni 25% /TiO 2 PC sensor exhibits an ultrafast optical response time of 3 s and a recovery time of 12 s with exceptional repeatability and reliability. Moreover, the CAU-1Ni 25% /TiO 2 PC sensor displays exceptional selectivity toward H 2 by comparing with CO 2 , NH 3 , and O 2 , which is important for practical hydrogen detection applications. This work presents incorporating Ni into CAU-1 enhances hydrogen adsorption energy through electronic structure modulation, enabling the CAU-1Ni 25% /TiO 2 PC to achieve rapid optical sensing response to hydrogen, which establish a new pathway for developing high-efficiency photonic crystal-based optical hydrogen sensors.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2025
·
IF 2.1
Guangjie Chen,Guangying Chen,Ge Gao,Li Sze Lai,Swee Pin Yeap,Wee Horng Tay,Helei Liu
The utilization of biphasic absorbents for CO2 capture can substantially reduce energy consumption. This study investigated the mass transfer kinetics and thermodynamics of the phase separation absorption process using 1-amino-2-propanol (MIPA)/sulfolane (TMS) biphasic solvents. 13C NMR spectroscopy was employed to analyze the species in different CO2-loaded solutions. The direction and extent of species transfer during the absorption process were elucidated and the phase separation mechanism was explored. CO2 absorption kinetics were studied in a wetted-wall column, and the physicochemical properties and the absorption reaction kinetics data under different experimental conditions were obtained. Results showed the overall mass transfer coefficient (KG) was 1.6 times higher than 30 wt % MEA and twice that of DETA/TMS solution. NMR and kinetic data analysis revealed that phase separation is mainly contributing to the deterioration of the mass transfer process in biphasic solution, reducing the liquid-film mass transfer coefficient (KL) by 63.2% during the phase separation stage. Furthermore, the desorption heats of different biphasic solvents with identical amine concentrations were measured using a microcalorimeter. Findings indicated that TMS did not alter the thermodynamic properties of the solution. Since only the CO2-rich phase is regenerated, the energy consumption is significantly reduced compared to that of MEA.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Tingting Wang,Xinhua Zhao,Zhenxu Li,Yi Zhang,Zhu Zhang,Dachi Yang
The newly-emerged gas sensing detection of 1-octen-3-ol biomarker is expected to indirectly monitor foodborne illnesses caused by Salmonella-contaminated meat, which requires high selectivity and a ppb-concentration detection limit. Here, surface-doped ruthenium over tungsten oxide nanocubes (Ru/WO 3 NCBs) have been synthesized by a wet-chemical approach for highly selective and ppb-concentration 1-octen-3-ol sensing. As-prepared Ru/WO 3 NCBs exhibit a polycrystalline structure and consist of NCBs that have an average side length of ∼131 nm, and the Ru/WO 3 NCBs show a high sensing response toward 5 ppm 1-octen-3-ol (R a /R g = 406), excellent selectivity, 50 ppb detection limit, 70% relative humidity tolerance, and 54 days stability at 220 o C. The excellent sensing performance might be attributed to the electronic and chemical sensitization of Ru, which thickens the electron depletion layer and increases adsorbed oxygen to promote interfacial electron transfer and 1-octen-3-ol oxidation. Practically, Ru/WO 3 NCBs have been integrated into a sensing device to simulate detecting 1-octen-3-ol, which has the potential for Salmonella detection in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Songtao Li,Tianxing Lv,Xiaxia Fan,Mangmang Feng,Meng Zhou,Zhuoyu Li
Background Chemotherapy remains the main treatment for colon cancer, but the development of resistance has limited its efficacy. There is an urgent need to exploit a more effective therapeutic strategy. Alpha-linolenic acid (ALA), as an essential nutrient for the human body, has been found to increase the therapeutic effect of chemotherapy drugs. Purpose Exploring the potential mechanism of alpha linolenic acid reversing chemotherapy resistance in colon cancer. Results ALA reverses chemoresistance in colorectal cancer and suppresses stemness in resistant colorectal cancer cells. SPP1 functions as a key mediator in ALA-induced reversal of chemoresistance; its downregulation disrupts the interaction with the downstream receptor CD44, thereby mitigating chemotherapy resistance. Subsequently, ALA inhibits the expression of SOX2, a transcription factor that promotes SPP1 transcription. Further analysis revealed that decreased SPP1 expression leads to reduced levels of FAP, a marker of cancer-associated fibroblasts (CAFs), and that a functional interaction exists between SPP1 and FAP. Interestingly, ALA disrupted the interaction between SPP1 and FAP, thereby preventing CAFs accumulation. In vivo studies confirmed that ALA reverses colorectal cancer chemoresistance via the SOX2/SPP1/FAP signaling axis. Conclusion The study demonstrates that ALA in quinoa depresses the stemness of colorectal cancer and reduces the number of CAFs via SPP1, thereby reversing chemotherapy resistance. These findings highlight ALA as a promising dietary-based adjuvant for enhancing chemotherapy efficacy in colorectal cancer and provide new mechanistic insights into targeting tumor stemness and stromal remodeling.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Jin-Hui Shi,Qi-Long Tang,Jin-Hong Wang,Yan-Lan Long,Sai-Feng Zhao,Zhen Zhao,Wan-Ting Xie,Zi-Ming Li,Hao-Ming Lu,Tian-Xiao Gao,Zhen-Zhen Fang,Ti Zhou,Bo-Xing Li,Xia Yang,Guo-Quan Gao,Wei-Wei Qi
Maintenance of glutamate homeostasis is essential for synaptic plasticity and cognition. Disrupted glutamate-glutamine cycling causes chronic excitotoxicity, a key driver of cognitive deficits in Alzheimer's disease (AD), though regulatory mechanisms remain unclear. Pigment epithelium-derived factor (PEDF), a neuroprotective protein declining with age, is demonstrated here to play a novel role in synaptic glutamate clearance. Analysis of peripheral blood samples from 19 patients with AD and 75 non-dementia control subjects revealed lower levels of PEDF in patients, and loss of PEDF correlates with cognitive decline. PEDF-deficient mice exhibit defective learning and memory, and higher susceptibility to AD. Furthermore, PEDF deficiency impaired synaptic plasticity and dendritic spine morphology. Mechanistically, PEDF inhibits ubiquitin-proteasome-dependent degradation of astrocytic glutamate transporter-1 (GLT-1) and normally guarantees elimination of synaptic glutamate by modulating the protein kinase C signaling pathway. Strikingly, restoring PEDF rescued cognitive deficits in a mouse model of AD, and upregulation of GLT-1 rescued cognitive impairment in PEDF-deficient mice. Collectively, these findings reveal PEDF is a physiologic regulator of synaptic glutamate homeostasis. Targeting PEDF deficiency-induced neural impairment may provide a novel avenue for the development of new therapeutic applications for neurodegenerative diseases associated with glutamate-induced excitotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ning Li,Chang Liu,Li Niu,Xu Li,Jacko Feng,Zhiming Liu
In this study, we examined the plasticizing effects of 1-butyl-3-Methylimidazolium Chloride (BmimCl), glycerol, and their combination on chitosan (CS) films. Additionally, we examined the effect of plasticizers for chitosan films structure and physicochemical properties of CS films by FTIR, XRD, SEM and mechanism of action of plasticizers on the structure of CS films. The results indicated that the interaction between BmimCl and chitosan is mainly ionic interaction and hydrogen bonding, while the interaction between glycerol and CS is mainly hydrogen bonding. The utilization of a 1:1 mixture of BmimCl and glycerol as a plasticizer exhibits a synergistic impact on the film samples. In dry conditions, the elongation at break of the CS/BmimCl 0.5 /Gly 0.5 film reached 17.47 %, representing a 33 % and 78 % increase compared to the CS/BmimCl and CS/Gly films, respectively. In moist environments, the mechanical strength of CS/BmimCl 0.5 /Gly 0.5 film was significantly enhanced to 35.09 MPa compared to the CS/BmimCl and CS/Gly films. Notably, the elongation at break of the films remained at 37.05 %, without compromising their flexibility properties.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
·Vol. 37
·期 20
·2405686
·
IF 26.8
Heng Zhang,Yiying Zhang,Yong Zhang,Bo Chen,Jingwen Guo,Yu Du,Jiajun Li,Fuwei Zhou,Fengyi Guo,Yongxin Zhang,Zixiang Zhao,Hangkai Xie,Zhixin Zhang,Tianqi Wang,Wuyi Qi,You Song,Fucong Fei,Xuefeng Wang,Fengqi Song
MnBi 2n Te 3n+1 is a representative family of intrinsic magnetic topological insulators, in which numerous exotic phenomena such as the quantum anomalous Hall effect are expected. The high-quality crystal growth and magnetism manipulation are the most essential processes. Here a modified chemical vapor transport method using a feedback-regulated strategy is developed, which provides the closed-loop control of growth temperature within ± 0.1 °C. Single crystals of MnBi 2 Te 4 , MnBi 4 Te 7 , and MnBi 6 Te 10 are obtained under different temperature intervals respectively, and show variable tunability on magnetism by finely tuning the growth temperatures. Specifically, the cold-end temperatures not only vary the strength of antiferromagnetic coupling in MnBi 2 Te 4 , but also induce magnetic ground state transitions from antiferromagnetism to ferromagnetism in MnBi 4 Te 7 and MnBi 6 Te 10 . In MnBi 2 Te 4 with optimized magnetism, quantized transport with Chern insulator state can be easily replicated. These results provide a systematic picture for the crystal growth and the rich magnetic tunability of MnBi 2n Te 3n+1 family, providing richer platforms for the related researches combining magnetism and topological physics.
✓ 已验证
DOI
PubMed
高影响因子
IMMUNITY
·
2024
·
IF 25.5
Jiping Sun,Youqin Zhang,Qingbing Zhang,Lin Hu,Linfeng Zhao,Hongdong Wang,Yue Yuan,Hongshen Niu,Dongdi Wang,Huasheng Zhang,Jianyue Liu,Xujiao Feng,Xiaohui Su,Ju Qiu,Jing Sun,Heping Xu,Catherine Zhang,Kathleen Wang,Yan Bi,Edgar G. Engleman,Lei Shen
Adipose tissue group 2 innate lymphoid cells (ILC2s) help maintain metabolic homeostasis by sustaining type 2 immunity and promoting adipose beiging. Although impairment of the ILC2 compartment contributes to obesity-associated insulin resistance, the underlying mechanisms have not been elucidated. Here, we found that ILC2s in obese mice and humans exhibited impaired liver kinase B1 (LKB1) activation. Genetic ablation of LKB1 disrupted ILC2 mitochondrial metabolism and suppressed ILC2 responses, resulting in exacerbated insulin resistance. Mechanistically, LKB1 deficiency induced aberrant PD-1 expression through activation of NFAT, which in turn enhanced mitophagy by suppressing Bcl-xL expression. Blockade of PD-1 restored the normal functions of ILC2s and reversed obesity-induced insulin resistance in mice. Collectively, these data present the LKB1-PD-1 axis as a promising therapeutic target for the treatment of metabolic disease.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）