COLLOIDS AND SURFACES B-BIOINTERFACES
·
2013
·
IF 3.554
Jinfeng Dou,Haiqun Zhang,Xiuju Liu,Mengyu Zhang,Guangxi Zhai
A mixed micelle that comprised of monomethylol poly(ethylene glycol)-poly( d , l -lactic acid) (MPP), d -α-tocopheryl polyethylene glycol 1000 succinate (TPGS) and stearic acid grafted chitosan oligosaccharide(CSO-SA) copolymers was developed to enhance the oral absorption of docetaxel (DTX). DTX-loaded MPP/TPGS/CSO-SA mixed polymeric micelles (MPMs) were prepared with thin film hydration method and characterized in terms of morphology, size, zeta potential, encapsulation efficiency, critical micellization concentration, and in vitro stability in media modeling physiological conditions. The in vitro release of docetaxel from the mixed micelles was studied with dialysis method. The oral bioavailability studies were conducted in rats and the pharmacokinetic parameters were evaluated. The results showed that DTX-loaded MPP/TPGS/CSO-SA MPMs had a mean diameter of 34.96 nm and exhibited spherical shape under transmission electron microscopy. The drug loading of DTX in the mixed micelles was 19.15%. The critical micellization concentration of MPP/TPGS/CSO-SA copolymer was 2.11 × 10 −5 M, and the size of mixed micelles in gastric fluid (pH 1.6) for 2 h and simulated intestinal fluid (pH 6.5) for 6 h showed no significant change. The in vitro release study showed that DTX-loaded MPP/TPGS/CSO-SA MPMs exhibited slower release characteristics compared to DTX solution. The oral bioavailability of the DTX-loaded MPP/TPGS/CSO-SA MPMs was increased by 2.52 times compared to that of DTX solution. The current results encourage further development of DTX mixed polymeric micelles as the oral drug delivery system.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Haiyan Wang,Ximei Zhang,Peng Kang,Xiuxiu Cui,Guo Hao,Zhongfu Wang,Bei Han,Xin Lv,Jing Zhang,Wupeng Ge
Oligosaccharides and N/O-glycans are abundant in human milk and have numerous biological functions (for instance sialylated glycans provide sialic acid for the growth of infant brains), but their variation trends during lactation need further exploration. Qualitative and quantitative analyses of oligosaccharides and N/O-glycans in human milk at different lactation stages (from 7 days to 8 months) were performed using UHPLC-ESI-MS/MS. Thirty-four oligosaccharides, twenty-three N-glycans, and six O-glycans were identified. Oligosaccharides showed the highest abundance in human colostrum and decreased with the progression of lactation, and the abundance of N/O-glycans fluctuated as lactation progressed, while a high abundance of sialylated oligosaccharides and sialylated N/O-glycans was observed in human colostrum. These findings provide evidence for breastfeeding support and contribute to the development of infant formula supplemented with human milk glycans.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Yang Lu,Juan Liu,Anqi Tong,Yongling Lu,Lishuang Lv
People are at high risk of exposure to endogenous and exogenous acrolein (ACR). ACR can cause a multitude of illnesses, including cardiovascular disease, Alzheimer’s disease, and diabetes. In this study, we investigated the reaction pathway of cardamonin (CAR) or alpinetin (ALP) with ACR and the interconversion of CAR and ALP in vitro at 37 °C in phosphate-buffered saline using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Subsequently, ACR adducts of CAR, ALP, and their metabolites, for example, CAR-ACR-1, ALP-ACR, mono-ACR-pinocembrin chalcone (PIN-ACR), and mono- and di-ACR-naringenin (NAR-ACR and NAR-2ACR), were detected in urine samples, but only CAR-ACR-1 and ALP-ACR were detected in fecal samples from the CAR- and ALP-treated mouse groups using ultraperformance liquid chromatography–MS/MS, respectively. Quantitative analyses showed that CAR, ALP, and their metabolites markedly scavenged ACR in a dose-dependent manner in vivo. Furthermore, we also found that the metabolites of CAR or ALP remained and promoted the ACR-trapping ability.
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Food Chemistry-X
·
2024
·
IF 6.5
Hongbin Yang,Fuqiang Wang,Yingqin Li,Yake Guo,Xiuhua Tang,Shuailei Gu,Haihong Chen,Chaohai Pang,Yanxia Li,Jiali Zhang,Weihong Ma,Jiashui Wang
Avocado is widely grown in tropical and subtropical regions of China. Diverse germplasms have been generated through natural hybridization and selective breeding. Here, to screen high-quality avocado germplasms, we characterized the nutritional quality of 95 avocado germplasms grown in China based on metabolomics. The oil content of the 95 avocado germplasms was 2.18 %–16.60 % and followed a normal distribution. We further profiled nine fatty acids, 16 hydrolyzed amino acids, and eight mineral elements in avocado fruit, which varied widely among different germplasms. Correlation analysis revealed significant positive correlations between fatty acid components, as well as between amino acid components and between mineral elements. Clustering analysis and evaluation of the 95 avocado germplasms identified 14 germplasms with high nutritional quality. These findings provide a basis for evaluation of avocado fruit quality and utilization of high-quality avocado germplasms, as well as important implications for the breeding of avocado.
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MOLECULAR THERAPY
·
2024
·
IF 12.1
Xiaozhen Kang,Yifeng Han,Mengdi Wu,Yuxin Li,Peng Qian,Chuning Xu,Zhengyun Zou,Jie Dong,Jiwu Wei
Tumor necrosis factor (TNF) has been recognized as an immune activation factor in tumor immunotherapy. Our study demonstrated that TNF blockade markedly enhanced the antitumor efficacy of oncolytic adenovirus (AdV) therapy. To minimize systemic side effects, we engineered a recombinant oncolytic AdV encoding a TNF inhibitor (AdV-TNFi) to confine TNF blockade within the tumor microenvironment (TME). AdV-TNFi significantly improved therapeutic outcomes across various solid tumor models, including four murine and two golden hamster cancers. Immune cell profiling identified CD8 + T cells as the primary mediators of AdV-TNFi-induced antitumor effects, rather than CD4 + T or NK cells. Additionally, AdV-TNFi significantly decreased the infiltration of suppressive myeloid-derived immune cells within the TME and promoted long-term antitumor immune surveillance. Further investigation indicated that TNFR2, more than TNFR1, is pertinent to the immunosuppressive TME, with a recombinant AdV-encoding anti-TNFR2 demonstrating comparable antitumor efficacy to AdV-TNFi. Moreover, AdV-TNFi enhanced the antitumor efficacy of gemcitabine and immune checkpoint blockades (ICBs), such as anti-PD-L1 and anti-TIGIT antibodies, in pancreatic carcinoma and the anti-EGFR antibody in colon carcinoma. In conclusion, intratumoral blockade of the TNF/TNFR2 axis using AdV augments cancer immunotherapy efficacy while mitigating the risks associated with systemic TNF or TNFR2 suppression, warranting further clinical investigation.
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JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2012
·Vol. 65
·期 1
·149-155
·
IF 2.175
Zhang Yan-Hui,Zhang You-Jin,Guo Yan-Lei,Li Wen-Juan,Yu Chao
ObjectivesWith the growing popularity of herbal and natural medicinal products, attention has turned to possible interactions between these products and pharmaceutical drugs. In this study, we examined whether astragaloside IV (AGS-IV) could inhibit the activity of CYP1A2 in rat liver microsomes in vitro and in vivo.MethodsThe effect of AGS-IV on CYP1A2 activity was investigated using probe substrates: phenacetin in vitro and theophylline in vivo. Phenacetin was incubated in rat liver microsomes with or without AGS-IV, and the mechanism, kinetics and type of inhibition were determined. The inhibitory effect of AGS-IV on CYP1A2 activity in rats was also determined using theophylline in vivo. The pharmacokinetics of theophylline were observed after a single or week-long treatment with AGS-IV.Key findingsAGS-IV was found to be a competitive inhibitor with a Ki value of 6.29 μm in vitro. In the multiple-pretreatment rat group, it was found to have a significantly higher area under the concentration–time curve (AUC) for theophylline, as well as a lower apparent oral total body clearance value (CL/F). In contrast, no significant difference in metabolism of theophylline was found for the single pretreatment group.ConclusionsThese findings suggest that AGS-IV is a potent inhibitor of CYP1A2. This work offers a useful reference for the reasonable and safe use of clinically prescribed herbal or natural products to avoid unnecessary herb–drug interactions.
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INORGANIC CHEMISTRY
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2021
·
IF 5.165
Shen-Jing Ji,Dong Zhang,Nian-Tzu Suen
Transition metal-based intermetallics are promising electrocatalysts for replacing the commercial Pt metal in the hydrogen evolution reaction (HER). In this work, RENi2 and RERu0.25Ni1.75 (RE = Pr, Tb, and Er) were synthesized and their electrocatalytic HER activities were explored. Among undoped compounds, PrNi2 exhibits the best performance and requires an overpotential of 55 mV, while partially replacing Ni with Ru element (PrRu0.25Ni1.75) can greatly reduce the overpotential to 20 mV at a current density of 10 mA/cm2. Such enhancement was recognized that belongs to their extrinsic property, and their intrinsic HER activities were similar after normalizing the electrocatalytic surface area. Further investigation on ScM2 and ScRu0.25M1.75 (M = Co and Ni) suggests that doping Ru element in ScCo2 will significantly enhance antibonding character around the Fermi level (EF) and weaken hydrogen adsorption energy. On the other hand, the antibonding population for ScNi2 and ScRu0.25Ni1.75 is similar at EF, which accounts for their close intrinsic HER activities.
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LANGMUIR
·
2022
·
IF 4.331
Ying Li,Maosen Wang,Yinghui An,Kaijun Li,Zhaojie Wei,Kun Bo,Pinlu Cao,Mingyi Guo
Wettability alteration of the shale surface is a potential strategy to address wellbore instability issues arising from shale hydration. In this study, we have explored an oil-in-water (o/w) nanoemulsion, in which soluble silicate (lithium silicate and potassium methyl silicate) as the aqueous phase and organosilanes (3-methacryloxypropyltrimethoxysilane (KH570) and n-octyltriethoxysilane (n-OTES)) as the oil phase, as a shale inhibitor via forming a hydrophobic “artificial borehole shield” in situ on shale surfaces to maintain wellbore stability in high-temperature drilling operations. The shale dispersion test showed the highest shale recovery of nanoemulsion was up to 106.4% compared to that of water (20%), and recovered shale cuttings remained at the original integrity after hot rolling at 180 °C, indicating superior inhibition performance and resistance to elevated temperatures. Moreover, recovered shale cuttings manifested water repellency upon reimmersion in water, ascribed to the hydrophobic film, preventing water from permeating into the shale. The results of the contact angle measurement elucidated that the film wettability, from hydrophilic to superhydrophobic (ranging from 9.6–154°), can be achieved by altering the n-OTES-to-KH570 weight ratio from 0.2 to 2.25, and the film with the highest hydrophobicity (154°) and the lowest surface energy (3.17 mJ·m–2) can be obtained at a ratio of 1.3. Scanning electron microscopy images demonstrated that the superhydrophobic film was composed of tightly stacked reticulate nanofilaments with a diameter of 7–17 nm and several micrometers in length and overlapped well-distributed nanospheres with a diameter of 30 nm. X-ray diffraction and Fourier transform infrared spectroscopy confirmed the film was crystalline silica grafted with long-chain alkylsiloxane. It is assumed that the unique micronanostructure combined with the siloxane modification contributed to the hydrophobicity. Consequently, this study provides a potential alternative solution for wellbore stabilization in deep well drilling engineering by employing nanoemulsion as a shale hydration inhibitor via forming a protective film with controllable wettability. Furthermore, it can be conferred a practical application due to easily available, less hazardous, and cost-effective materials.
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MATERIALS & DESIGN
·
2020
·
IF 6.289
Yuefeng Gao,Xinxiang Pan,Sai Xu,Zhijian Liu,Junsheng Wang,Kezhen Yu,Chengfa Wang,Haichao Yuan,Sen Wu
Sensitive on-line detection of Cu 2+ in lubricating oil of marine power plant is important for displaying equipment wear status and fault pre-judgment in real time. However, there are few reports on in-situ, portable and highly sensitive Cu 2+ detection devices in organic media before. In this report, CsPbBr 3 perovskite quantum dots (PQDs) are employed as fluorescence probe for Cu 2+ detection in microfluidic chip. The size effect of the CsPbBr 3 QDs on the detection performances is firstly investigated. To obtain preferable sensitivity and detection limit, polymethyl methacrylate opal photonic crystals (PMMA OPCs) film is developed as microfluidic sensor substrate, which can tremendously improve the fluorescence intensity of PQDs as high as 26-fold. The enhancement effect can be attributed to the coupling between photonic crystal stopband effect and the excitation light field. Moreover, the use of photomultiplier module for fluorescence signal measurement enables the detection device to be portable. The novel strategy offers the advantages of higher sensitivity (8.62 μM –1 ) and lower detection limit (0.40 nM). Otherwise, this sensor also provides wide detection range and good selectivity, thus, it will have potential in oil quality in-situ detection and mechanical wear prediction in the future.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Qidan Cai,Huaidong Peng,Jiang Meng,Youshao Yan,Yu Zeng,Paul C.H. Li,Yue Sun
In this study, a three-phase laminar flow microfluidic chip (TPL chip) combined with HPLC was developed for monitoring free and total concentrations of paclitaxel (PTX) in blood simultaneously. A diluted whole blood sample (aqueous phase) was introduced into the chip, ethyl acetate (organic phase) was introduced into the chip for extraction, and an interphase was used to prevent the blood sample from coming into direct contact with the organic phase. Because only free drug can quantitatively diffuse into the organic extraction phase and the free drug fraction has a linear relationship with the dilution factor of blood, both the free and total drug concentrations can be obtained by detecting the concentration of paclitaxel in the organic extraction phase. The governing factor such as flow rate for extraction was optimized. Docetaxel was used as an internal standard. The reliability of the quantitative diffusion of molecules in the TPL chip was proved by the methodological investigation of PTX in PBS sample, which showed a good linearity in the concentration range of 0.5 - 100 µg/mL and a detection limit of 7 ng/mL. Good repeatibilities for retention time (RSD of PTX is 1.23%, docetaxel is 1.14%, n = 5) and peak area ratio of PTX to docetaxel (RSD is 4.38%) were obtained. For blood sample analysis, only 100 µL of sample was needed and whole pretreatment was finished in 35 min, and a recovery of 94~117% were obtained. The provided method showed advantages in fast analysis speed, minimum sample handing, and potential ability of automation, and integration.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Yaru Wang,Jianjun Zhao,Xianqiang Xiong,Shengwei Liu,Yiming Xu
Anatase TiO 2 is the most studied environmental photocatalyst, but its efficiency is still not high enough to enable application. Herein we report a positive effect of Ni 2+ ions on the photocatalytic reaction in neutral aqueous solution. On addition of 1 mM Ni(ClO 4 ) 2 , the rates of phenol oxidation on TiO 2 and 0.5 wt.% Pt/TiO 2 increased by factors of 2.1 and 8.0, respectively. Meanwhile, the formation of organic intermediate was reduced, and the removal of total organic content was enhanced. Interestingly, Ni 2+ concentration did not change with irradiation time. Moreover, a maximum rate of phenol degradation was observed with Ni 2+ ions, occupying half the surface of TiO 2 or Pt/TiO 2 , where Ni(OH) + was more active than Ni(OH) 2 . X-ray photoelectron spectroscopy identified a Ni 3+ species produced in absence of phenol. A (photo)electrochemical study revealed that Ni 2+ oxidation was favored over H 2 O oxidation, whereas Ni 2+ and Pt inhibited and facilitated O 2 reduction, respectively. It is proposed that the adsorbed Ni(OH) + on TiO 2 is oxidized to Ni 3+ by the photogenerated holes of TiO 2 , followed by regeneration through phenol oxidation. The Ni 2+ -catalyzed organic oxidation then promotes the Pt-catalyzed O 2 reduction, and vice visa, resulting into the greatly improved efficiency of charge separation. This work highlights the possibility of Ni 2+ ions as the hole mediator of organic oxidation over a semiconductor.
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Journal of Materials Chemistry A
·
2019
·Vol. 7
·期 40
·23055-23063
·
IF 10.733
Fangxin Mao,Yan-Huan Jin,Peng Fei Liu,Pengfei Yang,Le Zhang,Luyang Chen,Xiao-Ming Cao,Jinlou Gu,Hua Gui Yang
Metal–organic frameworks (MOFs) have exhibited huge potential in the field of CO2 electroreduction but highly stable and active ones are still scarce. This study reports a (5,10,15,20-tetrakis(4-carboxyphenyl)porphyrinato)-Fe(III) chloride (FeTCPPCl) co-building UiO-66 electrocatalyst for CO2 reduction in an aqueous solution. In situ X-ray absorption spectroscopy (XAS) measurements characterized its robust structure under catalysis. The unique framework of UiO-66 provides the available proton facilitator for improving CO2 reduction activity on iron porphyrin, and a concerted proton–electron transfer (CPET) mechanism might be considered for the catalytic pathway, achieving the highest faradaic efficiency of nearly 100% at an overpotential of 450 mV for turning CO2 to CO in the reported MOFs.
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危险品化学品经营许可证（带存储）